In this study, we used 2-(diethexyphosphoryl)acetic acid as a common template molecule and Fe₃O₄@SiO₂ as support material to prepare a superparamagnetic core/shell molecular imprinting polymer (MIP), Fe₃O₄@SiO₂@MIP, which has multiple recognition sites and increased adsorption capacity. Then, a biomimetic fluorescence immunoassay method was developed for the simultaneous determination of methyl parathion, chlorpyrifos, and trichlorfon using the Fe₃O₄@SiO₂@MIP as biomimetic antibody and quantum dots as label. The factors that influenced the performance of this method were optimized. Under optimal conditions the limits of detection of the method were 0.21 ± 0.021, 0.44 ± 0.069 and 0.32 ± 0.033 μg/L for methyl parathion, chlorpyrifos, and trichlorfon, respectively. To evaluate the accuracy of the method, apple and orange samples spiked with methyl parathion, chlorpyrifos, and trichlorfon were analysed, and the recoveries ranged from 73.1% to 119.3%. Finally, this method was applied to detect three pesticides in pear, carrot, kiwifruit, and banana samples, and the results indicated a good correlation with those obtained from gas chromatography method.