A scientific justification, focused on the development of the sustainability of feed ingredients and farm animals’ ecosystems, is required. Thus, it is crucial to search for alternative feed materials from natural sources for potential applications. The aim of this study was to evaluate the prospective utilization of freshwater Cladophora glomerata (C. glomerata) as an alternative source of protein and other essential nutrients in animals’ feed. For this purpose, chemical analysis was performed on collected biomass samples from the Lithuanian rivers, Dubysa (B1), Šventoji (B2), Nevėžis (B3), and Jūra (B4). Microelements (Ca > K > N > P > Mg), trace elements (Zn > Cu), and heavy metals (Cr > Ni > Pb > Cd) have not exceeded permissible levels. The crude protein content of C. glomerata biomass ranged from 16% to 21.5% DM. The essential amino acid profile excelled, with the highest total amino acid amount of 140.99 g/kg in B4. The highest total presence of polyunsaturated fatty acids (11.71%) as well as the ratio of polyunsaturated to saturated fatty acids (0.22) was observed in B1. The lowest ratio of omega-6/omega-3 was in B1 (1.30). As a result of bioaccumulation, C. glomerata could serve as a source of proteins, as well as amino and fatty acids, implying that biomass could be an alternative and a beneficial component of animal feed.

This study was aimed to investigate the effect of feed supplemented with betaine on broiler chickens’ growth and slaughter performance, breast muscle histomorphometric and physicochemical properties, oxidative status and amino acid content. A total of 1000 1-day-old Ross 308 broiler chickens were divided into four treatments. Control group chickens were fed with standard compound diet (SCD), the chickens from experimental groups B1, B2 and B3 receiving SCD supplemented with 1 g/kg (B1), 2 g/kg (B2) and 3 g/kg (B3) betaine anhydrous, respectively. Each treatment had five replicate pens. Feeding test results showed that betaine reduced broilers’ mortality but increased feed conversion ratio (p < .05). Forty broiler chickens (5 weeks old) were slaughtered and slaughter performance showed that 2 g/kg betaine inclusion improved breast muscle percentage and yield (p < .05). Betaine dosage of 1 g/kg into feed increased breast muscle fibre areas (p < .05). Betaine affected some physicochemical properties: higher a* and the highest drip loss in B2; the highest cooking losses in B1, B2; the highest shear force and fat content in SCD; the highest amounts of ashes in B1, B2 (p < .05). Lower malondialdehyde levels were observed in all betaine-treated groups (p < .05), except B1 fresh meat samples. The highest total amino acid content and a greater amount of essential amino acids were obtained in SCD breast muscles (p < .05), except equally highest amounts of methionine were found in both SCD and B3 samples (p < .05). However, according to our study results, betaine, as a methyl group donor, in broiler chicken diets cannot replace methionine as an essential amino acid.

Freshwater macroalgae produces a wide range of bioactive compounds, and interest in utilizing its biomass is growing rapidly. Meanwhile, exploiting renewable sources for biomass collection could lead to more sustainable biotechnological chains. The aim of this study was to investigate Cladophora glomerata biomass from Lithuanian rivers as a potential raw material for biotechnology. For this reason, phenolic compound profiles, antioxidant activity and pigment concentrations were determined in macroalgal biomass samples collected from the following four Lithuanian rivers: Dubysa (B1), Šventoji (B2), Nevėžis (B3) and Jūra (B4). The highest total phenolic compound content was determined in B3 (1.32 mg GAE/g). Three phenolic acids were identified, namely gallic (12.94–35.13 µg/g), p-hydroxybenzoic (23.97–29.05 µg/g) and p-coumaric (1.79–6.46 µg/g). The results indicate significant C. glomerata antioxidant activity; the highest reducing power reached 0.737 AU (B3), the total antioxidant content was 1.47 mg Trolox/g (B3), DPPH and ABTS radical scavenging was 11.09% (B3) and 97.86% (B1) and FRAP activity 20.86 µmol/L (B3). The content of pigments ranged from 0.56-0.74, 0.39–0.57, 0.17–0.23 to 0.11–0.17 mg/g in chlorophyll a, b, carotenoids, and lutein, respectively. To conclude, C. glomerata macroalgal biomass may have the potential to act as a functional raw material, as several groups of bioactive compounds and antioxidant activities were observed.

Today’s challenges in the animal husbandry sector, with customers’ demand for more beneficial products, encourage the development of strategies that not only provide more sustainable production from the field to the table but also ensure final product functionality. Thus, the current research was aimed at replacing some traditional feed raw materials in rabbit diets with C. glomerata biomass to improve the functionality of meat. For this purpose, thirty weaned (52-d-old) Californian rabbits were assigned to 3 dietary treatments: standard compound diet (SCD), SCD + 4% C. glomerata (CG4), and SCD + 8% C. glomerata (CG8). At the end of the feeding trial, 122-d-old rabbits were slaughtered, longissimus dorsi (LD) and hind leg (HL) muscles were dissected post-mortem, and moisture, protein, and lipid profiles were determined. Results revealed that CG4 treatment can increase protein (22.17 g/kg), total (192.16 g/kg) and essential (threonine, valine, methionine, lysine, and isoleucine) amino acid levels in rabbit muscles. Both inclusions gradually reduced fat accumulation in muscles (CG8 < CG4 < SCD) but improved the lipid profile’s nutritional value by decreasing saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) and increasing polyunsaturated fatty acids (PUFA). As the dose of C. glomerata increased, the level of lipid oxidation decreased. Biomass supplementation enhanced PUFA/SFA and h/H levels while decreasing thrombogenicity index (TI) and atherogenic index (AI) levels in rabbit muscles, potentially contributing to the prevention of heart disease. Overall, dietary supplementation with C. glomerata biomass may be a more beneficial and sustainable nutritional approach to functionally enhancing rabbit meat.

Maintaining meat quality is essential to sustainable livestock management. Therefore, identifying alternative feed materials while considering consumer acceptance is crucial. So, the aim of this study was to evaluate the effect of C. glomerata-biomass-supplemented feeds on rabbit muscles’ physical properties, sensory profiles, and evaluators’ emotional responses to them. A total of thirty 52-day-old weaned Californian breed rabbits were randomly allocated to one of three dietary treatments: standard compound diet (SCD), SCD supplemented with 4% C. glomerata (CG4), or SCD supplemented with 8% C. glomerata (CG8). After the 122-day-old rabbits were slaughtered, post-mortem dissection of the rabbit Longissimus dorsi (LD) and hind leg (HL) muscles was conducted. The physical and histomorphometric features, sensory analyses, and emotional responses to the rabbit’s muscles were determined. Study results revealed CG4 and CG8 treatments significantly increased rabbit muscle moisture, while CG8 increased cooking losses in HL muscles (p < 0.05). Moreover, both CG treatments reduced the darkness and redness of fresh and cooked rabbit muscles compared to SCD (p < 0.05). CG8 treatment compared to SCD resulted in longer LD muscle fibers (p < 0.05). Evaluators discovered that the average scores for each sensory description of rabbit meat are acceptable and that consuming CG8-HL muscles can increase happiness based on emotional responses. Consequently, replacing traditional feed materials in rabbit feed with C. glomerata can lead to not only more sustainable production but also more consumer-acceptable rabbit meat.