Anisomeles indica (L.) kuntze is widely used in folk medicine against various disorders including allergy, sores, inflammation, and fever. This research investigated the antinociceptive, anxiolytic and sedative effects of A. indica methanol extract. The antinociceptive activity was assessed with the acetic acid-induced writhing test and formalin-induced flicking test while sedative effects with open field and hole cross tests and anxiolytic effects with elevated plus maze (EPM) and thiopental-induced sleeping time tests were assayed. Computer aided (pass prediction, docking) analyses were undertaken to find out the best-fit phytoconstituent of total 14 isolated compounds of this plant for aforesaid effects. Acetic acid treated mice taking different concentrations of extract (50, 100, and 200 mg/kg, intraperitoneal) displayed reduced the writhing number. In the formalin-induced test, extract minimized the paw licking time of mice during the first phase and the second phase significantly. The open field and hole-cross tests were noticed with a dose-dependent reduction of locomotor activity. The EPM test demonstrated an increase of time spent percentage in open arms. Methanol extract potentiated the effect of thiopental-induced hypnosis in lesser extent comparing with Diazepam. The results may account for the use of A. indica as an alternative treatment of antinociception and neuropharmacological abnormalities with further intensive studies. The compound, 3,4-dihydroxybenzoic acid was found to be most effective in computer aided models.

Objective: Present study aims to investigate both qualitative and quantitative phytochemicals presence, antioxidant, thrombolytic, α-amylase and cytotoxic activity of a Bangladeshi plant Steudnera colocasiifolia K. Koch leaf ethanolic extract. Methods: Phytochemical screening was accomplished by using established methods. Thrombolytic assay was conducted through clot lysis method. Alpha amylase inhibition assay was made by modified enzyme inhibitory action and cytotoxicity was studied by brine shrimp lethality test. Data was analyzed by One Way Analysis of Variance (ANOVA) using statistical software Statistical Package for Social Science (SPSS, Version 22.0, IBM corporation, NY) followed by student ‘t’ test with GraphPad Prism Data Editor for Windows, Version 6.0 (GraphPad software Inc., San Diego, CA). Results: Primary screening shows the presence of major metabolites such as alkaloid, flavonoid, tannin, saponins, steroid, quinone, cellulose and glycosides while the quantitative analysis elicits the presence of magnificent amount of flavonoid (70.60 ± 0.23), proanthocyanidin (64.46 ± 0.58), flavonol (71.22 ± 0.07) and total antioxidant (66.40 ± 0.26) in the extract. The thrombolytic effect of S. colocasiifolia was found to be 35.16% and this extract had good α-amylase inhibitory activity (IC₅₀=2.16 ± 0.06 mg/ml) as compared to Acarbose. The extract was found to have a LC₅₀ value of 305.2 μg/ml in Brine shrimp lethality bioassay. Conclusion: Results demonstrate the Steudnera colocasiifolia has a very good prospect to be studied further for its extended antioxidative and α-amylase inhibitory effects. Key words: Steudnera colocasiifolia, Antioxidant, Thrombolytic, α-amylase, Cytotoxic activity.

The presence of ¹⁴ N nucleus in the scalar coupling network results in a 3-fold decrease of ¹⁵ N T ₁ and polarization values for all ¹⁵ N sites in ¹⁵ N ₂ -metronidazole versus ¹⁵ N ₃ -metronidazole in SABRE hyperpolarization in microtesla fields.