We aimed in this study utilize environmental indicators as a quantitative method to evaluate and discuss the nitrogen (TN) and phosphorus (TP) flux by a production stage grow-out (termination) of Nile tilapia (Oreochromis niloticus) in fishpond. The TN and TP load, the mass balance, the input of TN and TP via feed and the converted nutrients in fish biomass are the environmental indicators applied in this study. During the production cycle (128 days), the system exported 15,931 g TN and 4,189 g TP that were related to the amount of feed supplied (r Pearson = 0.8825 and r = 0.8523, respectively), corroborated by the feed conversion ratio (1.61:1). The indicators showed that 26% TN and 45% TP were reversed into fish biomass, 62% TN and 40% TP were retained in the fishpond, and 12% TN and 15% TP were exported via effluent. The largest contribution of nutrients generated by the system and exported via effluent was observed in phase III and IV. This result is supported by the feed conversion ratio 2.14 and 2.21:1 obtained at this phase, a fact explained by the amount of feed offered and the fish metabolism. Application of environmental indicators showed to be an efficient tool to quantify flux of TN and TP produced during the grow-out period of Nile tilapia and therefore, guide management practices more sustainable. Concerning the environmental sustainability of the activity the implementation of best management practices such as the better control of the feed amount offered would lead to a smaller loss of TN and TP to the water. Furthermore, the use of better quality feeds would allow greater nutrient assimilation efficiency.

Blooms of cyanobacteria are frequent in Brazilian water reservoirs used for drinking water. The warning for the presence of potential toxin-producing cyanobacteria is typically based on time-consuming microscopy, rather than specific molecular detection of toxic genes in cyanobacteria. In this study, we developed a quantitative PCR assay for the detection of cyanobacteria producing the neurotoxin saxitoxin (STX). The assay targets the sxtA gene in the sxt gene cluster. Potential and dominant STX-producers in the Itupararanga reservoir were the genera Raphidiopsis, Aphanizomenon and Geitlerinema. Numbers of the sxtA gene varied from 6.76 × 10³ to 7.33 × 10⁵ cells mL⁻¹ and correlated positively with SXT concentrations in the water. Concentrations of STX and the sxtA gene also correlated positively with TN:TP ratio and pH, but correlated negatively with inorganic nutrients and turbidity, confirming that regulation of the SXT production was impacted by environmental variables. In contrast, the occurrence of another cyanotoxin, microcystin, did not correlate with any environmental variables. The developed qPCR assay was found to be a rapid and robust approach for the specific quantification of potential STX-producing cyanobacteria and should be considered in future investigations on toxic cyanobacteria to provide an early warning of potential toxin episodes.

INTRODUCTION: Frog farming, if not well managed, may cause environmental damages. The use of antibiotics, the organic discharge and the introduction of exotic species can disseminate risks such as eutrophication, changes in the water quality and organic pollution, factors that affect the human consumption. AIM: Evaluating the water quality of a bullfrog farming system, discussing their relations to production and the environment based on the current legislation. METHODS: Sampling was performed on a monthly basis from November 2006 to March 2007 during growth and fattening phases of bullfrog (Lithobates catesbeianus). Sample sites were distributed according to the water flow: upstream from the mixing zone, affluent (supply water), bay, effluent, mixing zone and downstream from the mixing zone. In the field, pH, electrical conductivity, dissolved oxygen, temperature and turbidity were measured. In laboratory, nitrogen, phosphorus and chlorophyll a concentrations were analyzed. RESULTS: The concentration of nutrients was determiner for water quality in the bay and its effluent. According to the current legislation, the effluent exceeded the limits for total phosphorus (> 0.030 mg L-1) and total nitrogen (> 1.27 mg L-1). Other variables presented acceptable values in light of the current laws. CONCLUSION: The high values of nutrients and other factors such as conductivity and turbidity are proportional to the animal growth due to the inadequate management practices evidenced by feed conversion rate. The following management options are proposed: maintaining the flow and decreased density of animals; maintaining the flow and density storage with adequate control of the food supply.

Freshwater reservoirs constitute an important source of drinking water, but eutrophication and higher temperatures increase the risk of more frequent blooms of cyanobacteria, including species that produce toxins. To improve the prediction of cyanotoxin episodes, we studied the annual occurrence of potential microcystin (MC) and saxitoxin (STX)-producing cyanobacteria in the Lobo reservoir, São Paulo State, Brazil. Relationships among environmental variables, cyanobacterial biomass, numbers of the mcyE and sxtA genotypes (genes encoding production of MC and STX, respectively), and concentrations of MC and STX were determined to address variables applicable for monitoring and predicting the dynamics of cyanobacteria and cyanotoxins in the reservoir. Microscopy confirmed the presence of potentially toxin-producing cyanobacteria at all sampling times, and qPCR detection showed the occurrence of both mcyE and sxtA in most samples. Concentrations of MC and STX were generally low (MC 0–1.54 µg L⁻¹; STX 0.03–0.21 µg L⁻¹). The highest MC level exceeded the recommended limit for human intake of 1 µg L⁻¹. The abundance of the mcyE and sxtA genes, as well as the toxin concentrations, were positively correlated with the biomass of Phormidium and Raphidiopsis. Among environmental variables, the abundance of potential toxic cyanobacteria was mainly affected by P limitation (high TN:TP ratios). Our data show that detection of the mcyE and sxtA genotypes serves as a useful and reliable predictor of toxin episodes but might be combined with chemical toxin detection to form an environmental toolbox for cyanotoxin monitoring.

The phosphorus and nitrogen discharge via effluent of intensive trout farming system was quantified through the use of environmental indicators. The nutrient loads, the mass balance, the estimated amount of nutrients in feed and the amount of nutrients converted in fish biomass were calculated based on the concentrations of phosphorus (P) and nitrogen (N) in the feed and in the water. Of the offered feed, 24.75 kg were available as P and 99.00 kg as N, of these, 9.32 kg P (38%) and 29.12 kg N (25%) were converted into fish biomass and 15.43 kg P (62%) and 69.88 kg N (75%) were exported via effluent. The loads and the mass balance show the excessive discharge of nutrients via effluent, corroborated by the feed conversion ratio (2.12:1) due to the low efficiency of feed utilization, therefore, it is proposed the use of this zootechnical parameter as environmental indicator. In addition, feed management practices are not adequate, highlighting the low frequency of feeding during the day, excessive amount and low quality of feed offered. These results demonstrate the need for adequate feed management and the need for careful monitoring of effluent.