Environmental factors and climate are the primary factors influencing the microbial colonization and deterioration of cultural heritage in outdoor environments. Hence, it is imperative to investigate seasonal variations in microbial communities and the biodeterioration they cause. This study investigated the surfaces of sandstone sculptures at Wat Umong Suan Phutthatham, Chiang Mai, Thailand, during wet and dry seasons using culture-dependent and culture-independent approaches. The fungi isolated from the sandstone sculptures were assessed for biodeterioration attributes including drought tolerance, acid production, calcium crystal formation, and calcium precipitation. The results show that most of the fungal isolates exhibited significant potential for biodeterioration activities. Furthermore, a culture-independent approach was employed to investigate the fungal communities and assess their diversity, interrelationship, and predicted function. The fungal diversity and the communities varied seasonally. The functional prediction indicated that pathotroph–saprotroph fungi comprised the main fungal guild in the dry season, and pathotroph–saprotroph–symbiotroph fungi comprised the dominant guild in the wet season. Remarkably, a network analysis revealed numerous positive correlations among fungal taxa within each season, suggesting a potential synergy that promotes the biodeterioration of sandstone. These findings offer valuable insights into seasonal variations in fungal communities and their impacts on the biodeterioration of sandstone sculptures. This information can be utilized for monitoring, management, and maintenance strategies aimed at preserving this valuable cultural heritage.

Phi Man Long Long Rak Cave, located in Mae Hong Son Province, northern Thailand, is a prehistoric burial site containing ancient wooden coffins that have undergone biodeterioration, likely due to fungal activity. Both culture-dependent and culture-independent approaches were employed to characterize fungal communities and assess their roles in wood degradation. Culture-dependent analysis identified five Aspergillus isolates from the wooden coffins, most of which produced cellulolytic and hemicellulolytic enzymes; some isolates also produced organic acids, indicating significant degradative potential. Culture-independent analysis revealed a community dominated by Aspergillus, together with additional taxa such as Penicillium and Ceriporia that were not detected by cultivation, highlighting greater community diversity and demonstrating the complementarity of the two methods. Functional prediction indicated a predominance of saprotrophic fungi. The presence of shared dominant taxa between soil and coffin-associated substrates suggests ecological connectivity at the soil–coffin interface, although the direction of dispersal cannot be determined from the present data. All tested fungicides inhibited fungal growth, with the highest efficacy observed in the formulation containing the highest proportion of active components. Taken together, these findings provide insights into fungal biodeterioration processes and inform conservation strategies.

Paraquat is a widely used herbicide known to adversely affect honeybee physiology; however, its impact on gut-associated biological processes remains poorly understood. Therefore, intricate effects of paraquat on honeybees highlight the need for a comprehensive understanding of its specific biological consequences. This study aimed to evaluate the gut microbiome composition and metabolomic profiles of honeybees following oral exposure to sublethal (LD₂₅) and toxic (LD₅₀) doses of paraquat, assessed 48 h post-exposure to represent acute exposure. Survival rate and sucrose consumption were monitored to assess toxicity. Honeybee gut samples were analyzed using 16S rRNA gene amplicon sequencing and metabolomic approaches. Both paraquat treatments resulted in increased mortality (p < 0.0001) and reduced sucrose consumption (p = 0.00503). In contrast, gut bacterial community composition remained largely unchanged (p > 0.05). Metabolomic analysis revealed dose-dependent alterations, particularly in metabolites associated with carbohydrate and energy metabolism, including oxaloacetic acid and pyruvic acid. This study provides the first integration of 16S rRNA gene amplicon sequencing and metabolomic analyses to evaluate the effects of paraquat exposure in honeybees. The findings indicate that paraquat-induced stress primarily exhibits as host metabolic reprogramming rather than changes in microbiome composition. These results provide insights into the mechanisms underlying honeybee responses to herbicide stress and may contribute to the development of strategies for honeybee protection.