Background: We assessed the effects of a 6-week, low-volume Nordic hamstring exercise (NHE) intervention on hamstring flexibility, muscle mechanical properties and eccentric and isometric isokinetic knee flexion strength in recreationally active adults. Methods: Eighteen recreationally active adults were randomized into an NHE intervention group (IG; n = 9; females/males: 3/6; mean ± SD, age: 24.1 ± 1.3 years) and control group (CG; n = 9; females/males: 5/4; mean ± SD, age: 23.5 ± 1.8 years). The NHE intervention involved a progressive, supplementary training program performed initially one (weeks 1 and 2) and then two times per week over a 6-week period. The number of repetitions per session increased from 15 to 36 repetitions/week. The CG maintained their usual exercise routine over the same period. Standard goniometry, myotonometry, and isokinetic dynamometry (60°/s) were used to measure hamstring flexibility, muscle properties and isometric and eccentric isokinetic strength prior to and five days following the intervention. Results: The Linear Mixed Methods analysis identified a significant group × time interactions for isometric torque (IG: +5% vs. CG: −12%, p = 0.022) and flexibility (IG: +1% vs. CG: +7%, p = 0.023). Peak eccentric torque (IG: +7% vs. CG: −7%, p = 0.053) and muscle mechanical properties remained unchanged over the intervention period. Conclusions: Six weeks of low-volume NHE training marginally improved isometric and eccentric hamstring strength in recreationally active adults without changing hamstring flexibility or mechanical properties. The findings may have important implications for performance enhancement and hamstring injury risk reduction during high-intensity recreational sports.