The increase in microbial sequenced genomes from pure cultures and metagenomic samples reflects the current attainability of whole-genome and shotgun sequencing methods. However, software for genome visualization still lacks automation, integration of different analyses, and customizable options for non-experienced users. In this study, we introduce GenoVi, a Python command-line tool able to create custom circular genome representations for the analysis and visualization of microbial genomes and sequence elements. It is designed to work with complete or draft genomes, featuring customizable options including 25 different built-in color palettes (including 5 color-blind safe palettes), text formatting options, and automatic scaling for complete genomes or sequence elements with more than one replicon/sequence. Using a Genbank format file as the input file or multiple files within a directory, GenoVi (i) visualizes genomic features from the GenBank annotation file, (ii) integrates a Cluster of Orthologs Group (COG) categories analysis using DeepNOG, (iii) automatically scales the visualization of each replicon of complete genomes or multiple sequence elements, (iv) and generates COG histograms, COG frequency heatmaps and output tables including general stats of each replicon or contig processed. GenoVi’s potential was assessed by analyzing single and multiple genomes of Bacteria and Archaea. Paraburkholderia genomes were analyzed to obtain a fast classification of replicons in large multipartite genomes. GenoVi works as an easy-to-use command-line tool and provides customizable options to automatically generate genomic maps for scientific publications, educational resources, and outreach activities. GenoVi is freely available and can be downloaded from https://github.com/robotoD/GenoVi.

Nowadays, conventional agriculture farms lack high-level automated management due to the limited number of installed sensor nodes and measuring devices. Recent progress of the Internet of Things (IoT) technologies will play an essential role in future smart farming by enabling automated operations with minimum human intervention. The main objective of this work is to design and implement a flexible IoT-based platform for remote monitoring of agriculture farms of different scales, enabling continuous data collection from various IoT devices (sensors, actuators, meteorological masts, and drones). Such data will be available for end-users to improve decision-making and for training and validating advanced prediction algorithms. Unlike related works that concentrate on specific applications or evaluate technical aspects of specific layers of the IoT stack, this work considers a versatile approach and technical aspects at four layers: farm perception layer, sensors and actuators layer, communication layer, and application layer. The proposed solutions have been designed, implemented, and assessed for remote monitoring of plants, soil, and environmental conditions based on LoRaWAN technology. Results collected through both simulation and experimental validation show that the platform can be used to obtain valuable analytics of real-time monitoring that enable decisions and actions such as, for example, controlling the irrigation system or generating alarms. The contribution of this article relies on proposing a flexible hardware and software platform oriented on monitoring agriculture farms of different scales, based on LoRaWAN technology. Even though previous work can be found using similar technologies, they focus on specific applications or evaluate technical aspects of specific layers of the IoT stack.

Internet of Things (IoT) technologies are opening new opportunities and services for different smart grid applications such as advanced meter infrastructures (AMI), distributed energy resources, and electric vehicles. Among these applications, AMIs represent the first step toward future smart grid implementation by enabling the reading and recording of electric power consumption on request or a schedule. In this study, the main focus is designing an IoT-based architecture to support AMIs' daily reporting and billing in a residential grid. First, the network modeling for AMI is discussed. Long range (LoRa) technology, as one of the promising candidates for long range low power wide area networks (LPWANs), has been studied and discussed. A comprehensive analysis of the LoRa-based architecture is given for a case study of an actual residential grid, Puerto Montt, Chile. The results are analyzed and discussed for packet delivery ratio, energy consumption, throughput, number of collisions, and frequency distribution.

Nowadays, legacy electrical distribution systems are migrating to a new modern electric grid with the capability of supporting different applications such as advanced metering infrastructure (AMI), distributed energy resources, and electric vehicles. Among these applications, AMI is playing an important role in delivering data from customers to power utilities, supporting reliable real-time monitoring, and remote operation of power quality data and voltage profile. The AMI consists of smart meters, data aggregation points (DAPs), the utility control center, and communication networks. Appropriate network planning plays an essential role in facilitating the exchange of data between consumers and power utilities as well as accommodating new smart grid applications and future growth. This work proposes an optimal placement of DAPs for AMI based on machine learning clustering techniques in residential grids. Network partitioning is introduced to create sub-networks and graph algorithms generate a deployment topology given optimization constraints. A new measurement metric called coverage density is considered to indicate neighborhood area networks (NAN) zones with the appropriate coverage. Three real scenarios of NAN are considered: urban, suburban, and rural. The proposed algorithm is evaluated and compared with conventional heuristic optimization methods with respect to average and maximum distance between smart meters and DAPs, coverage density, and execution time.

Multi-band elastic optical networks are a promising alternative to meet the bandwidth demand of the ever-growing Internet traffic. In this letter, we propose a family of band allocation algorithms for multi-band elastic optical networks. Employing simulation, we evaluate the blocking performance of 3 algorithms of such a family and compare their performance with the only heuristic proposed to date. Results show that the three new algorithms outperform the previous proposal, with up to one order of magnitude improvement. We expect these results to help advance the area of dynamic resource allocation in multi-band elastic optical networks.

A deep reinforcement learning (DRL) approach is applied, for the first time, to solve the routing, modulation, spectrum, and core allocation (RMSCA) problem in dynamic multicore fiber elastic optical networks (MCF-EONs). To do so, a new environment was designed and implemented to emulate the operation of MCF-EONs - taking into account the modulation format-dependent reach and intercore crosstalk (XT) - and four DRL agents were trained to solve the RMSCA problem. The blocking performance of the trained agents was compared through simulation to 3 baselines RMSCA heuristics. Results obtained for the NSFNet and COST239 network topologies under different traffic loads show that the best-performing agent achieves, on average, up to a four-times decrease in blocking probability with respect to the best-performing baseline heuristic method.