Carob (Ceratonia siliqua L.) is a tree species native to the Mediterranean region and belongs to the Fabaceae family. The tree is well-known for its sweet and nutritious fruits, which have been used for long time as a nutritious food. In addition to the edible fruits, the carob tree also produces seeds that are highly prized for their ability to produce carob gum (locust bean gum). The carob seed consists of three main components: the shell, the endosperm, and the embryo. The shell is the outermost layer of the seed, followed by the endosperm, which is the largest part of the seed and contains high levels of carbohydrates and proteins. The embryo is the smallest part of the seed and is rich on bioactive compounds. Carob seed constituents have attracted considerable attention due to their exceptional nutritional and therapeutic properties in various industries, including food, medicine, pharmaceuticals, cosmetics, and textiles. The high content of bioactive compounds in carob seeds, such as polyphenols, tannins, and flavonoids, is believed to be responsible for their antioxidant and anti-inflammatory properties. The use of carob seed constituents in the food industry is mainly due to their ability to act as thickeners and stabilizers in various foods. They are used as a substitute for other thickening agents such as guar gum and carrageenan, due to their superior properties. In the pharmaceutical industry, carob seeds have been found to have antidiabetic, antihyperlipidemic, and anticancer properties, among others. The cosmetics industry is also interested in the ingredients of carob seed, as they can improve hydration and elasticity of the skin. They are also used as a natural alternative to synthetic thickeners in cosmetic formulations. The textile industry has also recognized the potential of carob seed constituents, as they can be used as a natural dye and as a sizing agent to improve the strength and durability of textiles. In summary, carob seed constituents offer a wide range of applications in various industries, owing to their high content of bioactive compounds, excellent nutritional and therapeutic profile, and ability to act as thickeners, stabilizers, and antioxidants. This review has highlighted the latest findings on the chemical composition, applications, and health benefits of carob seed constituents.

Ceratonia siliqua L. is a medicinal plant that has long been used in traditional Moroccan medicine to treat many diseases. This study aimed to assess the impact of the stages of the immature phase of carob pulp (M1, M2, M3, M4, and M5) on phytochemical composition, antioxidant activity, and antidiabetic activity of Ceratonia siliqua L. The identification of the phenolic profile by HPLC-UV/MS-MS and the study of the antidiabetic effect by in silico, in vitro, and in vivo studies were carried out for extracts with high contents of phenolic compounds from immature wild carob pulp from the communes of Timoulit (TM), Bin Elouidane (AW), and Ouaouizerth (TG) in the province of Azilal in the Béni Mellal-Khénifra region. The results revealed a gradual increase in total sugar content over the pulp’s ripening period, reaching a value of 2134 ± 56.23 mg GE/100 g fresh weight (FW) for TG. The three locations showed peak values for total polyphenol content (TPC), total flavonoid content (TFC), and total condensed tannin (TCT) at the M2 stage. AW had the highest concentrations of TPC (3819 ± 226.4 mg GAE/100 g FM), TFC (1034 ± 57.08 mg QE/100 g FM), and TCT (1472 ± 28.46 mg CE/100 g FM). The DPPH assay (7892 ± 296.1 mg TE/100 g FM) and the FRAP assay (278.2 ± 7.85 mg TE/100 g FM) both demonstrated that the TG zone is a highly potent antioxidant zone. In contrast, the AW site exhibited a markedly elevated value of 725.4 ± 103.6 mg TE/100 g FM in the ABTS assay. HPLC-UV-MS/MS analysis showed that the methanolic extracts of immature carob pulp (MEICP) from the three areas contained several different chemical compounds. The most prevalent were 3-O-p-coumaroyl-5-O-caffeoylquinic acid, quercetin 3-methyl ether, gallic acid, and galloylquinic acid. Immature carob pulp extract (ICPE) from AW showed the strongest in vitro inhibition of pancreatic α-amylase (IC₅₀ = 0.405 µg/mL) and TG extracts were most potent against intestinal α-glucosidase (IC₅₀ = 0.063 µg/mL). In vivo, AW, TG, and TM extracts significantly reduced postprandial glycemia in rats, with AW having the greatest effect. These results highlight the antidiabetic potential of ICPE. The 3-O-p-Coumaroyl-5-O-caffeoylquinic acid showed better affinity for α-amylase compared to acarbose and interacted significantly with several amino acid residues of the enzyme. Similarly, this molecule and 3,4-Dicaffeoylquinic acid demonstrated a strong affinity for α-glucosidase, suggesting their potential as natural inhibitors of enzymes involved in carbohydrate metabolism. Most of the compounds are not substrates of P-glycoprotein and exhibited high intestinal absorption. Furthermore, the majority of these compounds did not act as inhibitors or substrates of CYP450 enzymes, reinforcing their suitability for development as oral medications. These results underscore the potential of immature carob pulp as a promising antidiabetic agent.

The aim of this study was to develop the procedure for the varietal discrimination of date palm fruit using image analysis and traditional machine learning techniques. The fruit images of ‘Mejhoul’, ‘Boufeggous’, ‘Aziza’, ‘Assiane’, and ‘Bousthammi’ date varieties, converted to individual color channels, were processed to extract the texture parameters. After performing the attribute selection, the textures were used to build models intended for the discrimination of different varieties of date palm fruit using machine learning algorithms from Functions, Bayes, Lazy, Meta, and Trees groups. Models were developed for combining image textures selected from a set of all color channels and for sets of textures selected for individual color spaces and color channels. The models, including combined textures selected from all color channels, distinguished all five varieties with an average accuracy reaching 98%, and ‘Bousthammi’ and ‘Mejhoul’ were completely correctly discriminated for the SMO (Functions) and IBk (Lazy) machine learning algorithms. By reducing the number of varieties, the correctness of the date palm fruit classification increased. The models developed for the three most different date palm fruit varieties ‘Boufeggous’, ‘Bousthammi’, and ‘Mejhoul’ revealed an average discrimination accuracy of 100% for each algorithm used (SMO, Naive Bayes (Bayes), IBk, LogitBoost (Meta), and LMT (Trees)). In the case of individual color spaces and channels, the accuracies were lower, reaching 97.3% for color space RGB and SMO and LMT algorithms for all five varieties and 99.63% for Naive Bayes and IBk for the ‘Boufeggous’, ‘Bousthammi’, and ‘Mejhoul’ date palm fruits. The results can be used in practice to develop vision systems for sorting and distinguishing the varieties of date palm fruit to authenticate the variety of the fruit intended for further processing.

An in-depth determination of date fruit properties belonging to a given variety can have an impact on their consumption, processing, and storage. The objective of this study was to characterize date fruits of the ‘Mejhoul’ variety using (i) objective and non-destructive image-analysis features and (ii) measurements of physicochemical parameters. Based on images acquired using a digital camera, more than 1600 texture parameters from the individual color channels L, a, b, R, G, B, X, Y, and Z, and 40 geometric characteristics (including linear dimensions and shape factors for each fruit), were determined. Additionally, pomological features, water content, water activity, color parameters (L*, a*, b*), total soluble solids (TSS), reducing sugars, and total sugars were measured. As a main result, the application of machine vision allowed for the correct detection of ‘Mejhoul’ dates and the determination of the image features. The differences in the values of the histogram’s mean (HMean texture) for individual color channels were determined. The ‘Mejhoul’ date fruit images in color channel a (aHMean equal to 145.88) and color channel b (bHMean: 145.49) were the brightest, and in channel Z they were the darkest (ZHMean: 4.23). Due to the determination of the elliptic shape factor (W₁) of 1.000 and the circular shape factor (W₂) of 0.110, the elliptical shape of the fruit was confirmed. On the other hand, ‘Mejhoul’ dates were characterized by a length of 47.3 mm, a diameter of 26.4 mm, flesh thickness of 6.25 mm, total soluble solids of 62.1%, water content of 28.0%, water activity of 0.652, hardness of 694 g, reducing sugars of 13.8%, and total sugars of 58.8%. Due to the determination of many image features and other parameters, this paper presents the first comprehensive characterization of ‘Mejhoul’ date fruits using a non-destructive imaging technique linked to some physicochemical quality attributes.