Sustainable Development (SD) is a fundamental objective in the European Union (EU) and transport is considered one of the key challenges necessary to achieve it. Although transport is mostly contested from the environmental dimension, an investigation of peer-reviewed literature along with EU policy documents suggests that the transport and accessibility (T&A) criteria of infrastructure, accessibility distance, and multimodality can positively contribute to SD. However, despite this synergetic relation between T&A and SD, a practical analysis of such enablers is unknown at the regional European level. Therefore, this study investigates the Mediterranean as a study area by analyzing 79 identified passenger ports as passenger transport land-sea interaction points. Based on open access data, port infrastructure and ship accessibility, hinterland accessibility, and multimodality are evaluated as the passenger T&A enablers for SD. Comparative geo-spatial analyses are also carried out among the passenger ports’ levels of enablers by using the data normalization method. These data driven comprehensive analytical results can bring added value to SD policy and planning initiatives in the Mediterranean. This study may also contribute to the development of relevant passenger port performance indicators for boosting port or regional competition and attractiveness towards SD.