The personalized approach in sports genetics implies considering the allelic variants of genes in polymorphic loci when adjusting the training process of athletes. The personalized approach is used both in sports genetics and in medicine to identify the influence of genotype on the manifestations of human physical qualities that allow to achieve high sports results or to assess the impact of genotype on the development and course of diseases. The impact of genes of the renin-angiotensin and kinin-bradykinin systems in the development of cardiovascular disease in athletes has not been defined. This study aims to determine the polymorphisms of four genes (ACE, BDKRB2, PPARGC1A and NOS3) and the total genetic score to reveal the predisposition to the formation of physical qualities in martial arts athletes with different athletic abilities. The products of these four genes are involved in the control of blood pressure. The allelic variants of these genes are associated with the development of the physical quality “endurance” and have an indirect influence on the formation of speed and power qualities. The total genetic score (TGS: from 0 to 100 arbitrary units) was calculated from the genotype score in each polymorphism. The athletes were divided into Group I with high and Group II with low qualifications depending on their sports success. Single nucleotide polymorphisms (SNPs) are identified through restriction endonucleases cleavage for PCR amplicons for discriminating between alleles of the target genes ACE (rs4646994), BDKRB2 (rs5810761), PPARGC1A (rs8192673) and NOS3 (rs1799983). Significant differences between the allelic variants of target genes and athletic ability were found between Group I and Group II for genotype G/G of NOS3 gene and genotypes Gly/Gly and Gly/Ser of PPARGC1A gene. The data obtained confirm that athletes with unfavourable genotypes are excluded in the screening phase because their endurance is not fully developed to the required level in martial arts. Martial arts athletes with the highest TGS have the highest skill level. Polymorphic loci of four genes whose products are involved in blood pressure control (ACE, BDKRB2, NOS3 and PPARGC1A) can be used in martial arts not only to determine predisposition to cardiovascular disease but also to predispose to the development of speed and power qualities and endurance. The total genetic score can serve as a tool for predicting athletic success.

Establishing sustainable plantations with genetic diversity equivalent to that of natural populations is vital for successful reforestation efforts. In this study, we present an innovative approach for selecting populations suitable for reforestation, taking into account their genetic uniqueness using Inter Simple Sequence Repeats (ISSR) markers. Our investigation focused on six populations of Picea obovata Ledeb and six populations of Larix sibirica Ledeb, collected from the Northern and Middle Urals. We found that the indicators of genetic diversity were significantly higher in L. sibirica compared to P. obovata, while the number of rare alleles was greater in Siberian spruce (R = 19). Among the P. obovata populations, the Cherdyn’s forestry exhibited notably high genetic diversity, and for L. sibirica, the Gainy’s forestry stood out in this regard. Moreover, the genetic subdivision of the six P. obovata populations (G_ST = 0.331) was higher than that of the six L. sibirica populations (G_ST = 0.177). To ensure optimal seed selection considering the genetic originality coefficient (GOC) and population differentiation, we recommend utilizing the P. obovata population from Gainy’s forestry with a GOC of 0.554 and the L. sibirica population from Cherdyn’s forestry with a GOC of 0.372. These populations harbor typical alleles characteristic of the research region, making them ideal candidates for seed selection. Furthermore, the specific alleles identified can serve as valuable markers for determining the geographic origin of P. obovata and L. sibirica wood, aiding in efforts to trace the sources of these species in forestry and trade practices.

Monitoring of potato varieties phytosanitary status is a necessary measure of its stable yield, since due to the vegetative propagation method, the accumulation of viruses increases over generations. Fourteen varieties of Solanum tuberosum L. grown in the Perm Territory were studied for infection with the potato viruses PVX (X) and PVY (Y). Among them six (Rosara, Impala, Aladdin, Lady Claire, Gala, Madeira) are varieties of foreign selection, and 8 varieties (Golubizna, Udacha, Krasavchik, Charodei, Nevsky, Otrada, Tescha, Elizaveta) — domestic selection. Real-time PCR method was used for material investigation, since it allows quick and efficient evaluation of plant material for the presence of viral infection. During real-time PCR Y potato virus was detected in all 90 samples of 14 studied potato varieties from three storage locations. Potato X virus was detected in 54 samples of 11 studied potato varieties from three different storage locations. It was established that the studied planting material of all 14 varieties of potatoes is infected with the Y virus, which affects crop yields to a greater extent. Private households are most susceptible to infection of planting material with X and Y potato viruses. Recommendations are given on reducing the viral diseases of potato planting material.

Background/Objectives: The spruces of the Picea abies–P. obovata complex have a total range that is the most extensive in the world flora of woody conifers. Hybridization between the nominative species has led to the formation of a wide introgression zone, which probably increases the adaptive potential of the entire species complex. This study aimed to search the genes associated with drought resistance, develop primers for the informative loci of these genes, identify and analyze SNPs, and establish the parameters of nucleotide diversity in the studied populations. Methods: The objects of this study were eight natural populations of the spruce complex in the Urals. Nucleotide sequences related to drought resistance spruce genes with pronounced single-nucleotide substitutions were selected, based on which 16 pairs of primers to their loci were developed and tested. Results: Based on the developed primers, six pairs of primers were chosen to identify SNPs and assess the nucleotide diversity of the studied populations. All selected loci were highly polymorphic (6 to 27 SNPs per locus). It was found that the Pic01 locus is the most variable (Hd = 0.947; π = 0.011) and selectively neutral, and the Pic06 locus is the most conservative (Hd = 0.516; π = 0.002) and has the most significant adaptive value. Conclusions: The nucleotide diversity data for the studied populations reveal similar values among the populations and are consistent with the literature data. The discovered SNPs can be used to identify adaptive genetic changes in spruce populations, which is essential for predicting the effects of climate change.

Background: Scots Pine is one of the main forest-forming species in boreal forests; it has great economic and ecological significance. This study aimed to develop and test primers for detecting nucleotide polymorphisms in genes that are promising for detecting adaptive genetic variability in populations of Pinus sylvestris in the Urals and adjacent territories. Objectives: The objects of the study were 13 populations of Scots Pine located in the Perm Territory, Chelyabinsk Region, and the Republic of Bashkortostan. Results: Sixteen pairs of primers to loci of potentially adaptively significant genes were developed, from which three pairs of primers were selected to detect the nucleotide diversity of the studied populations. The indicator of total haplotype diversity determined in the three studied loci varied from 0.620 (Pinus-12 locus) to 0.737 (Pinus-11 locus) and, on average, amounted to 0.662. The nucleotide diversity indicators in P. sylvestris in the study region were, on average, low (π = 0.004, θ_W = 0.013). Their highest values were found at the Pinus-12 locus (π = 0.005; θ_W = 0.032), and the lowest were found at the Pinus-15 locus (π = 0.003; θ_W = 0.002). This indicates that Pinus-15 is the most conserved of the three studied loci. In the three studied P. sylvestris loci associated with adaptation to environmental factors, 97 polymorphic positions were identified. The 13 populations of P. sylvestris are characterized by an average level of genetic diversity (Hd = 0.662; π = 0.004; θ = 0.013). Conclusions: The polymorphic loci of adaptively significant genes of P. sylvestris can help identify the adaptive potential of pine forests in conditions of increasing ambient temperatures.

Рожь озимая (Secale cereale L.) является важной продовольственной культурой России. Изучение генетического разнообразия зерновых культур проводится с использованием молекулярных маркеров. Для определения полиморфизма ДНК был использован метод выявления эффективных праймеров ISSR (Inter Simple Sequence Repeats). ISSR-праймеры отбирались по следующим параметрам: 1) наибольшее число полиморфных фрагментов ДНК; 2) четкая амплификация; 3) воспроизводимость при повторных ПЦР. Всего проанализировано 22 ISSR-праймера с тотальной ДНК S. cereale. Из них высокую эффективность продемонстрировали пять ISSR-праймеров для изученных пяти озимых сортов ржи посевной. Для молекулярно-генетического анализа S. cereale были отобраны следующие четыре динуклеотидных ISSR-праймера (M1 (AC)8CG, ISSR-5 (AG)8CA, CR-215 (CT)6TG, CR-218 (GA)6CC) и один тринуклеотидный ISSR-праймер X10 (AGC)6C. Выявленные ISSR-праймеры позволят определить генетическое разнообразие сортов озимой ржи, выращиваемых в условиях Среднего Урала.