Rafael Alarcon Rivera

Mr. Rafael Alarcon Rivera

Universidad de Lima, Lima, Peru

Research Focus & Metrics

Academic Career Length

7Years (2020-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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15 May 2023 in Foods
Verified
Journal Article
Open access
PDF file
NCNancy Chasquibol
Nancy Chasquibol
Billy Francisco Gonzales GarciaBilly Francisco Gonzales Garcia
Billy Francisco Gonzales Garcia
RRRafael Alarcon Rivera
Rafael Alarcon Rivera
ASAxel Sotelo
Axel Sotelo
JLJose Carlos Marquez Lopez
Jose Carlos Marquez Lopez
JPJusto Pedroche
Justo Pedroche
In this research, scallops (Argopecten purpuratus) visceral meal (SVM) and defatted meal (SVMD) were analysed for their proximal composition, protein solubility, and amino acid profile. Hydrolysed proteins isolated from the scallop’s viscera (SPH) were optimised and characterised using response surface methodology with a Box-Behnken design. The effects of three independent variables were examined: temperature (30–70 °C), time (40–80 min), and enzyme concentration (0.1–0.5 AU/g protein) on the degree of hydrolysis (DH %) as a response variable. The optimised protein hydrolysates were analysed for their proximal composition, yield, DH %, protein solubility, amino acid composition, and molecular profile. This research showed that defatted and isolation protein stages are not necessaries to obtain the hydrolysate protein. The conditions of the optimization process were 57 °C, 62 min and 0.38 AU/g protein. The amino acid composition showed a balanced profile since it conforms to the Food and Agriculture Organisation/World Health Organisation recommendations for healthy nutrition. The predominant amino acids were aspartic acid + asparagine, glutamic acid + Glutamate, Glycine, and Arginine. The protein hydrolysates’ yield and DH % were higher than 90% and close to 20%, respectively, with molecular weight between 1–5 kDa. The results indicate that the protein hydrolysates of scallops (Argopecten purpuratus) visceral by product optimised and characterised was suitable a lab-scale. Further research is necessary to study the bioactivity properties with biologic activity of these hydrolysates.

22 July 2022 in Antioxidants
Verified
Journal Article
Open access
PDF file
NCNancy Chasquibol
Nancy Chasquibol
RRRafael Alarcon Rivera
Rafael Alarcon Rivera
Billy Francisco Gonzales GarciaBilly Francisco Gonzales Garcia
Billy Francisco Gonzales Garcia
ASAxel Sotelo
Axel Sotelo
LLLourdes Nahir Landoni
Lourdes Nahir Landoni
MPM. Carmen Pérez-Camino
M. Carmen Pérez-Camino
Sacha inchi Plukenetia huayllabambana oil (SIPHO) was co-microencapsulated, by spray drying using gum arabic as a coating material, with antioxidant extracts of camu camu (Myrciaria dubia (HBK) McVaugh) (CCSE) and mango (Mangifera indica) (MSE) skins obtained by ultrasound–microwave-assisted extraction (UMAE). The physicochemical characteristics of the microcapsules, such as, particle size, morphology, and moisture, as well as the encapsulation efficiency, the fatty acid composition, and oxidative stability, were determined in order to select the best formulation for the design of functional powdered beverages. The formulation with the highest amounts of ω3 acids and polyphenols was used to prepare a functional powdered beverage that contained ω3 (52.74%), antioxidant activity (324.80 mg AAE/100 g powder), and acceptable sensory attributes.
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25 May 2023 in Foods
Verified
Journal Article
Open access
PDF file
NCNancy Chasquibol
Nancy Chasquibol
Billy Francisco Gonzales GarciaBilly Francisco Gonzales Garcia
Billy Francisco Gonzales Garcia
RRRafael Alarcon Rivera
Rafael Alarcon Rivera
ASAxel Sotelo
Axel Sotelo
GGGabriela Gallardo
Gabriela Gallardo
MPM. Carmen Pérez-Camino
M. Carmen Pérez-Camino
Sacha inchi (Plukenetia huayllabambana) oil was co-microencapsulated with natural antioxidant extracts (NAE), such as camu-camu (Myrciaria dubia (HBK) Mc Vaugh) fruit, Añil variety Andean potato (Solanum tuberosum andigenum, and elderberry fruit (Sambucus peruviana). Gum Arabic and the ternary combination of gum Arabic (GA) + maltodextrin (MD) + whey protein isolate (WPI) at different formulations were used as coating materials for the encapsulation process using spray-drying. The moisture content, particle size distribution and morphology, total phenolic content, antioxidant activity, fatty acid and sterol composition, oxidative stability, and shelf-life were evaluated. Co-microcapsules of sacha inchi (P. huayllabambana) oil with camu camu skin extract (CCSE) at 200 ppm encapsulated with GA + MD + WPI had the highest total polyphenol content (4239.80 µg GAE/g powder), antioxidant activity (12,454.00 µg trolox/g powder), omega-3 content (56.03%), β-sitosterol (62.5%), greater oxidative stability (Oxidation Onset temperature of 189 °C), higher shelf-life (3116 h), and smaller particle sizes (6.42 μm). This research enhances the knowledge to obtain microcapsules containing sacha inchi (P. huayllabambana) oil with natural antioxidant extracts that could be used for the development of functional foods. Further research is needed to study the potential interactions and their influence between the bioactive components of the microcapsules and the challenges that may occur during scale-up to industrial production.

4 December 2020 in Grasas y Aceites
Verified
Journal Article
Open access
PDF file
LLL. Landoni
L. Landoni
RRRafael Alarcon Rivera
Rafael Alarcon Rivera
LVL. Vilca
L. Vilca
NCN. Chasquibol
N. Chasquibol
MPM. Carmen Pérez-Camino
M. Carmen Pérez-Camino
GGG. Gallardo
G. Gallardo
The aim of this work was to obtain sacha inchi oil (SIO) microcapsules from two different species, Plukenetia volubilis L. (SIVO) and Plukenetia huayllabambana L. (SIHO), using different biopolymers as wall materials and spray drying technology. The physicochemical characteristics such as encapsulation efficiency, particle size, morphology and oxidative stability were analyzed in order to select the best formulation that could potentially be used as an ingredient in the development of functional food. Bulk SIO and four formulations were tested for each oil ecotype, using different encapsulating agents: maltodextrin (MD), Arabic gum (AG), whey protein concentrate (WPC) and modified starch HI-CAP®-100 (H). Microcapsules made of H presented the highest oxidative stability and encapsulation efficiency compared to AG, AG:MD or AG:MD:WPC formulations.

31 May 2024 in Antioxidants
Verified
Journal Article
Open access
PDF file
NCNancy Chasquibol
Nancy Chasquibol
ACAxel Alexandre Sotelo Contreras
Axel Alexandre Sotelo Contreras
MTMateo Tapia
Mateo Tapia
RRRafael Alarcon Rivera
Rafael Alarcon Rivera
Francisco M. GoycooleaFrancisco M. Goycoolea
Francisco M. Goycoolea
MPM. Carmen Pérez-Camino
M. Carmen Pérez-Camino
Cushuro (Nostoc sphaericum) polysaccharide was used to co-microencapsulate sacha inchi oil, natural antioxidant extracts from the oleoresin of charapita chili peppers (Capsicum frutescens L.) and grape orujo (Vitis vinifera L.). Encapsulation efficiency, moisture, particle size, morphology, oxidative stability, shelf-life, solubility, essential fatty acid profile, sterol content and antioxidant capacity were evaluated. The formulations with grape orujo extract showed higher oxidative stability (4908 ± 184 h), antioxidant capacity (4835.33 ± 40.02 µg Trolox/g ms), higher phenolic contents (960.11 ± 53.59 µg AGE/g ms) and a smaller particle size (7.55 µm) than the other formulations, as well as good solubility and a low moisture content. Therefore, grape orujo extracts can be used as natural antioxidants. The fatty acid composition (ω-3) remained quite stable in all the formulations carried out, which also occurred for sterols and tocopherols. In combination with gum arabic, grape orujo extract offered oxidative protection to sacha inchi oil during the first week of storage.

28 September 2023 in Biology and Life Sciences Forum
Verified
Conference Paper
Open access
PDF file
NCNancy Chasquibol
Nancy Chasquibol
ACAxel Alexandre Sotelo Contreras
Axel Alexandre Sotelo Contreras
RRRafael Alarcon Rivera
Rafael Alarcon Rivera
The trend in protein consumption is towards plant-based foods, for this reason a powdered beverage with a balanced profile of essential amino acids was developed, combining two different sources of protein, cushuro (Nostoc commune) and quinoa (Chenopodium quinoa). The cushuro is an ancient Andean cyanobacteria that grows over 3000 and 4000 m above sea level, and quinoa is an important Andean seed, and they are part of the basic diet of the Andean Peruvian population. The cushuro obtained from the region of Ancash-Peru was dried (60 °C, 18 h) and the flour was dissolved in 0.1 N HCl at 90 °C for 20 min to obtain a protein concentrate with 6.79% moisture, 0.16% fat, 71.03% protein, and good balance of essential amino acids. Quinoa flour presented 8.52% moisture, 4.84% fat, 12.90% protein, and essential amino acids such as phenylalanine, and lysine. Quinoa flour (65.45%), cushuro concentrated protein (4.76%), cocoa (17.85%), panela (11.90%), and stevia (0.05%), were mixed to obtain the powdered beverage. The results showed that powdered beverage presented an adequate balance of amino acids according to FAO/WHO, with 14.36% proteins, 72.53% carbohydrate, 5.19% fat, 716.6 mg/kg potassium, 319.8 mg/kg phosphorus, 139.2 mg/kg magnesium, 82.69 mg/100 g vitamin C, 1.49 µg/100 g vitamin B12, heavy metals below the detection evel (<0.050 mg/kg). The powdered beverage complied with the Peruvian legislation of the Healthy Law No. 30021 and with the microbiological requirements.