Lots of experimental studies have revealed the significant associations between lncRNAs and diseases. Identifying accurate associations will provide a new perspective for disease therapy. Calculation-based methods have been developed to solve these problems, but these methods have some limitations. In this paper, we proposed an accurate method, named MLGCNET, to discover potential lncRNA-disease associations. Firstly, we reconstructed similarity networks for both lncRNAs and diseases using top k similar information, and constructed a lncRNA-disease heterogeneous network (LDN). Then, we applied Multi-Layer Graph Convolutional Network on LDN to obtain latent feature representations of nodes. Finally, the Extra Trees was used to calculate the probability of association between disease and lncRNA. The results of extensive 5-fold cross-validation experiments show that MLGCNET has superior prediction performance compared to the state-of-the-art methods. Case studies confirm the performance of our model on specific diseases. All the experiment results prove the effectiveness and practicality of MLGCNET in predicting potential lncRNA-disease associations.

An emerging type of therapeutic agent, anticancer peptides (ACPs), has attracted attention because of its lower risk of toxic side effects. However process of identifying ACPs using experimental methods is both time-consuming and laborious. In this study, we developed a new and efficient algorithm that predicts ACPs by fusing multi-view features based on dual-channel deep neural network ensemble model. In the model, one channel used the convolutional neural network CNN to automatically extract the potential spatial features of a sequence. Another channel was used to process and extract more effective features from handcrafted features. Additionally, an effective feature fusion method was explored for the mutual fusion of different features. Finally, we adopted the neural network to predict ACPs based on the fusion features. The performance comparisons across the single and fusion features showed that the fusion of multi-view features could effectively improve the model’s predictive ability. Among these, the fusion of the features extracted by the CNN and composition of k-spaced amino acid group pairs achieved the best performance. To further validate the performance of our model, we compared it with other existing methods using two independent test sets. The results showed that our model’s area under curve was 0.90, which was higher than that of the other existing methods on the first test set and higher than most of the other existing methods on the second test set. The source code and datasets are available at https://github.com/wame-ng/DLFF-ACP.

Circular RNAs (circRNAs) are covalently closed single-stranded RNA molecules, which have many biological functions. Previous experiments have shown that circRNAs are involved in numerous biological processes, especially regulatory functions. It has also been found that circRNAs are associated with complex diseases of human beings. Therefore, predicting the associations of circRNA with disease (called circRNA-disease associations) is useful for disease prevention, diagnosis and treatment. In this work, we propose a novel computational approach called GGCDA based on the Graph Attention Network (GAT) and Graph Convolutional Network (GCN) to predict circRNA-disease associations. Firstly, GGCDA combines circRNA sequence similarity, disease semantic similarity and corresponding Gaussian interaction profile kernel similarity, and then a random walk with restart algorithm (RWR) is used to obtain the preliminary features of circRNA and disease. Secondly, a heterogeneous graph is constructed from the known circRNA-disease association network and the calculated similarity of circRNAs and diseases. Thirdly, the multi-head Graph Attention Network (GAT) is adopted to obtain different weights of circRNA and disease features, and then GCN is employed to aggregate the features of adjacent nodes in the network and the features of the nodes themselves, so as to obtain multi-view circRNA and disease features. Finally, we combined a multi-layer fully connected neural network to predict the associations of circRNAs with diseases. In comparison with state-of-the-art methods, GGCDA can achieve AUC values of 0.9625 and 0.9485 under the results of fivefold cross-validation on two datasets, and AUC of 0.8227 on the independent test set. Case studies further demonstrate that our approach is promising for discovering potential circRNA-disease associations.

Radiotherapy plays an important role in controlling the local recurrence of esophageal cancer after radical surgery. Segmentation of the clinical target volume is a key step in radiotherapy treatment planning, but it is time-consuming and operator-dependent. This paper introduces a deep dilated convolutional U-network to achieve fast and accurate clinical target volume auto-segmentation of esophageal cancer after radical surgery. The deep dilated convolutional U-network, which integrates the advantages of dilated convolution and the U-network, is an end-to-end architecture that enables rapid training and testing. A dilated convolution module for extracting multiscale context features containing the original information on fine texture and boundaries is integrated into the U-network architecture to avoid information loss due to down-sampling and improve the segmentation accuracy. In addition, batch normalization is added to the deep dilated convolutional U-network for fast and stable convergence. In the present study, the training and validation loss tended to be stable after 40 training epochs. This deep dilated convolutional U-network model was able to segment the clinical target volume with an overall mean Dice similarity coefficient of 86.7% and a respective 95% Hausdorff distance of 37.4 mm, indicating reasonable volume overlap of the auto-segmented and manual contours. The mean Cohen kappa coefficient was 0.863, indicating that the deep dilated convolutional U-network was robust. Comparisons with the U-network and attention U-network showed that the overall performance of the deep dilated convolutional U-network was best for the Dice similarity coefficient, 95% Hausdorff distance, and Cohen kappa coefficient. The test time for segmentation of the clinical target volume was approximately 25 seconds per patient. This deep dilated convolutional U-network could be applied in the clinical setting to save time in delineation and improve the consistency of contouring.

Medical image segmentation plays a crucial role in clinical diagnosis, treatment planning, and disease monitoring. The automatic segmentation method based on deep learning has developed rapidly, with segmentation results comparable to clinical experts for large objects, but the segmentation accuracy for small objects is still unsatisfactory. Current segmentation methods based on deep learning find it difficult to extract multiple scale features of medical images, leading to an insufficient detection capability for smaller objects. In this paper, we propose a context feature fusion and attention mechanism based network for small target segmentation in medical images called CFANet. CFANet is based on U-Net structure, including the encoder and the decoder, and incorporates two key modules, context feature fusion (CFF) and effective channel spatial attention (ECSA), in order to improve segmentation performance. The CFF module utilizes contextual information from different scales to enhance the representation of small targets. By fusing multi-scale features, the network captures local and global contextual cues, which are critical for accurate segmentation. The ECSA module further enhances the network’s ability to capture long-range dependencies by incorporating attention mechanisms at the spatial and channel levels, which allows the network to focus on information-rich regions while suppressing irrelevant or noisy features. Extensive experiments are conducted on four challenging medical image datasets, namely ADAM, LUNA16, Thoracic OAR, and WORD. Experimental results show that CFANet outperforms state-of-the-art methods in terms of segmentation accuracy and robustness. The proposed method achieves excellent performance in segmenting small targets in medical images, demonstrating its potential in various clinical applications.

Intracranial aneurysm (IA) is a vascular disease of the brain arteries caused by pathological vascular dilation, which can result in subarachnoid hemorrhage if ruptured. Automatically classification and segmentation of intracranial aneurysms are essential for their diagnosis and treatment. However, the majority of current research is focused on two-dimensional images, ignoring the 3D spatial information that is also critical. In this work, we propose a novel dual-branch fusion network called the Point Cloud and Multi-View Medical Neural Network (PMMNet) for IA classification and segmentation. Specifically, one branch based on 3D point clouds serves the purpose of extracting spatial features, whereas the other branch based on multi-view images acquires 2D pixel features. Ultimately, the two types of features are fused for IA classification and segmentation. To extract both local and global features from 3D point clouds, Multilayer Perceptron (MLP) and the attention mechanism are used in parallel. In addition, a SPSA module is proposed for multi-view image feature learning, which extracts more exquisite channel and spatial multi-scale features from 2D images. Experiments conducted on the IntrA dataset outperform other state-of-the-art methods, demonstrating that the proposed PMMNet exhibits strong superiority on the medical 3D dataset. We also obtain competitive results on public datasets, including ModelNet40, ModelNet10, and ShapeNetPart, which further validate the robustness and generality of the PMMNet.