Cicer arietinum L. is the third greatest widely planted imperative pulse crop worldwide, and it belongs to the Leguminosae family. Drought is the utmost common abiotic factor on plants, distressing their water status and limiting their growth and development. Chickpea genotypes have the natural ability to fight drought stress using certain strategies viz., escape, avoidance and tolerance. Assorted breeding methods, including hybridization, mutation, and marker-aided breeding, genome sequencing along with omics approaches, could be used to improve the chickpea germplasm lines(s) against drought stress. Root features, for instance depth and root biomass, have been recognized as the greatest beneficial morphological factors for managing terminal drought tolerance in the chickpea. Marker-aided selection, for example, is a genomics-assisted breeding (GAB) strategy that can considerably increase crop breeding accuracy and competence. These breeding technologies, notably marker-assisted breeding, omics, and plant physiology knowledge, underlined the importance of chickpea breeding and can be used in future crop improvement programmes to generate drought-tolerant cultivars(s).

Genome editing aims to revolutionise plant breeding and could assist in safeguarding the global food supply. The inclusion of a 12–40 bp recognition site makes mega nucleases the first tools utilized for genome editing and first generation gene-editing tools. Zinc finger nucleases (ZFNs) are the second gene-editing technique, and because they create double-stranded breaks, they are more dependable and effective. ZFNs were the original designed nuclease-based approach of genome editing. The Cys2-His2 zinc finger domain’s discovery made this technique possible. Clustered regularly interspaced short palindromic repeats (CRISPR) are utilized to improve genetics, boost biomass production, increase nutrient usage efficiency, and develop disease resistance. Plant genomes can be effectively modified using genome-editing technologies to enhance characteristics without introducing foreign DNA into the genome. Next-generation plant breeding will soon be defined by these exact breeding methods. There is abroad promise that genome-edited crops will be essential in the years to come for improving the sustainability and climate-change resilience of food systems. This method also has great potential for enhancing crops’ resistance to various abiotic stressors. In this review paper, we summarize the most recent findings about the mechanism of abiotic stress response in crop plants and the use of the CRISPR/Cas mediated gene-editing systems to improve tolerance to stresses including drought, salinity, cold, heat, and heavy metals.

Chickpea is an important leguminous crop with potential to provide dietary proteins to both humans and animals. It also ameliorates soil nitrogen through biological nitrogen fixation. The crop is affected by an array of biotic and abiotic factors. Among different biotic stresses, a major fungal disease called Fusarium wilt, caused by Fusarium oxysporum f. sp. ciceris (FOC), is responsible for low productivity in chickpea. To date, eight pathogenic races of FOC (race 0, 1A, and 1B/C, 2-6) have been reported worldwide. The development of resistant cultivars using different conventional breeding methods is very time consuming and depends upon the environment. Modern technologies can improve conventional methods to solve these major constraints. Understanding the molecular response of chickpea to Fusarium wilt can help to provide effective management strategies. The identification of molecular markers closely linked to genes/QTLs has provided great potential for chickpea improvement programs. Moreover, omics approaches, including transcriptomics, metabolomics, and proteomics give scientists a vast viewpoint of functional genomics. In this review, we will discuss the integration of all available strategies and provide comprehensive knowledge about chickpea plant defense against Fusarium wilt.

Flax, or linseed, is considered a “superfood”, which means that it is a food with diverse health benefits and potentially useful bioactive ingredients. It is a multi-purpose crop that is prized for its seed oil, fibre, nutraceutical, and probiotic qualities. It is suited to various habitats and agro-ecological conditions. Numerous abiotic and biotic stressors that can either have a direct or indirect impact on plant health are experienced by flax plants as a result of changing environmental circumstances. Research on the impact of various stresses and their possible ameliorators is prompted by such expectations. By inducing the loss of specific alleles and using a limited number of selected varieties, modern breeding techniques have decreased the overall genetic variability required for climate-smart agriculture. However, gene banks have well-managed collectionns of landraces, wild linseed accessions, and auxiliary Linum species that serve as an important source of novel alleles. In the past, flax-breeding techniques were prioritised, preserving high yield with other essential traits. Applications of molecular markers in modern breeding have made it easy to identify quantitative trait loci (QTLs) for various agronomic characteristics. The genetic diversity of linseed species and the evaluation of their tolerance to abiotic stresses, including drought, salinity, heavy metal tolerance, and temperature, as well as resistance to biotic stress factors, viz., rust, wilt, powdery mildew, and alternaria blight, despite addressing various morphotypes and the value of linseed as a supplement, are the primary topics of this review.

Drought stress is a universal crisis in sustaining the growth and production of major legumes, including the chickpea. Drought severely reduces the biomass of chickpea plants, with the effect on leaves appearing the most apparent. The aim of this study was to investigate, using various physiological and biochemical markers throughout the pod filling stage, how 78 desi chickpea genotypes tolerated drought stress. Most of the evaluated characteristics showed significant variations between control and drought treatments. The mean performance of most of the investigated parameters significantly decreased under moisture-stressed conditions. RWC, SWD, MSI, and CTD were investigated under terminal drought-stressed conditions. Except for saturated water deficit (SWD), all remaining characteristics declined with increasing stress. Genotypes SAGL152210, SAGL152252, SAGL152347, SAGL22-115, and JG11 were recognized as drought-tolerant based on physiological characteristics. Biochemical markers viz., protein content, total soluble sugar, lipid peroxidation, and proline content, had an impact on osmotic adjustment. Based on non-enzymatic biochemical traits, genotypes SAGL22-115, ICC4958, ICCV201108, ICCV201107, SAGL152252, and JG11 were identified for their capability to survive under drought-stressed conditions. H₂O₂ content, CAT, SOD, POD, APX, and DPPH were considered antioxidant agents. Genotypes SAGL152208, SAGL22-105, SAGL22-112, ICC201108, SAGL152278, SAGL152252, SAGL162371, SAGL162390, ICC 4958, and JG315 may be considered drought-tolerant based on antioxidant activities. These genotypes are believed to be better equipped with physio-biochemical mechanisms and antioxidant defense systems at the cellular level and can be used in breeding programs to breed drought-tolerant cultivar(s). They can also be screened in the future, allowing the line(s) that have remained consistent over time to be recognized and registered as drought-tolerant donors.

Background: Drought is the foremost environmental restraint that effects the growth and yield of chickpea. The mechanism of response to drought differs with genotype and growth stages of individual crop species. The activation of antioxidant enzymes is an alternate defensive system against oxidative stress that ultimately decide development of putative drought tolerant mechanism. Methods: Eighty-three chickpea genotypes were grown under normal and drought stress conditions and leaf samples were used to estimate different biochemical parameters including total sugar, lipid peroxidation (MDA), chlorophyll, proline and protein along with estimation of activities of different antioxidant enzymes viz., catalase (CAT) (EC 1.11.1.6), ascorbate peroxidase (APX) (EC 1.11.1.11), superoxide dismutase (SOD) and peroxidase (POX). Result: Positively significant correlation was found among proline under control with proline underwent stress (r=0.441), MDA under stress with proline under control (r=0.365) and MDA under control (r=0.336) at 1% level of significance. Positively significant correlation was also investigated between SOD under stressed condition with SOD under control (r=0.665), POX (0.449) and APX under stress (0.423), CAT under control (0.471) and CAT under stress condition (0.374) at 1% probability level. Heatmaps along with dendrograms represented expression levels of different antioxidant enzymes activities that showed variations among different genotypes. In conclusion. total sugar, proline and Malondialdehyde, have been increased under drought stressed condition whilst total chlorophyll and protein were decreased. While antioxidant enzymes viz., POX, APX, CAT and SOD levels increased under drought stressed conditions.