Aloe vera is effectively utilized to synthesize zinc oxide nanoparticles (Av-ZnO NPs), providing an alternative to traditional chemical and physical methods. This sustainable approach minimizes the environmental impacts and enhances their compatibility with herbal ecosystems. We comprehensively analyzed the optical, structural, morphological, and catalytic properties of Av-ZnO NPs using various analytical methods. The results indicated that the nanoparticles primarily exhibited a spherical shape. X-ray diffraction (XRD) revealed the successful formation of a highly crystalline hexagonal wurtzite structure, with an average size estimated at 12.2 nm. The antimicrobial properties of the Av-ZnO NPs indicated moderate antibacterial effectiveness. Using the DPPH free radical scavenging method, we evaluated the antioxidant properties, where the Av-ZnO NPs exhibited improved the radical scavenging efficiency, reflected by a lower IC₅₀ value compared to the plant extract. Additionally, we assessed the photocatalytic functionality through the degradation of methylene blue (MB) dye, finding that the Av-ZnO NPs achieved approximately 82.43% degradation in 210 min, demonstrating their potential for environmental remediation. These findings suggest that green-synthesized ZnO NPs could play a noteworthy role in various nanotechnology applications and biomedical fields, while also promoting environmental sustainability.