Drought and heat stresses are the major abiotic stress factors detrimental to maize (Zea mays L.) production. Much attention has been directed toward plant responses to heat or drought stress. However, maize reproductive stage responses to combined heat and drought remain less explored. Therefore, this study aimed to quantify the impact of optimum daytime (30°C, control) and warmer daytime temperatures (35°C, heat stress) on pollen germination, morpho‐physiology, and yield potential using two maize genotypes (“Mo17” and “B73”) under contrasting soil moisture content, that is, 100% and 40% irrigation during flowering. Pollen germination of both genotypes decreased under combined stresses (42%), followed by heat stress (30%) and drought stress (19%). Stomatal conductance and transpiration were comparable between control and heat stress but significantly decreased under combined stresses (83% and 72%) and drought stress (52% and 47%) compared with the control. Genotype “Mo17” reduced its green leaf area to minimize the water loss, which appears to be one of the adaptive strategies of “Mo17” under stress conditions. The leaf reflectance of both genotypes varied across treatments. Vegetation indices associated with pigments (chlorophyll index of green, chlorophyll index of red edge, and carotenoid index) and plant health (normalized difference red‐edge index) were found to be highly sensitive to drought and combined stressors than heat stress. Combined drought and heat stresses caused a significant reduction in yield and yield components in both Mo17 (49%) and B73 (86%) genotypes. The harvest index of genotype “B73” was extremely low, indicating poor partitioning efficiency. At least when it comes to “B73,” the cause of yield reduction appears to be the result of reduced sink number rather than the pollen and source size. To the best of our awareness, this is the first study that showed how the leaf‐level spectra, yield, and quality parameters respond to the short duration of independent and combined stresses during flowering in inbred maize. Further studies are required to validate the responses of potential traits involving diverse maize genotypes under field conditions. This study suggests the need to develop maize with improved tolerance to combined stresses to sustain production under increasing temperatures and low rainfall conditions.

The past few years have seen increased interest in unmanned aerial vehicle (UAV)-based hyperspectral imaging (HSI) and machine learning (ML) in agricultural research, concomitant with an increase in published research on these topics. We provide an updated review, written for agriculturalists, highlighting the benefits in the retrieval of biophysical parameters of crops via UAVs relative to less sophisticated options. We reviewed >70 recent papers and found few consistent results between similar studies. Owing to their high complexity and cost, especially when applied to crops of low value, the benefits of most of the research reviewed are difficult to explain. Future effort will be necessary to distill research findings into lower-cost options for end-users.

The wetland plant species, Phragmites australis, is present on every continent except Antarctica. Both native and non-native subspecies thrive in the USA with the non-natives quickly displacing native wetland plants. Along the Gulf Coast, Phragmites grows in very dense stands, and at heights of greater than 4.6 m, is usually the tallest grass species in a wetland, estuary, and marsh ecosystems. Phragmites is known to alter the ecology of these wetland systems making them less suitable as habitat for many species of flora and fauna. Furthermore, Phragmites presents a navigation hazard to smaller boats by impairing visibility along shorelines and around bends of canals and rivers. Management efforts targeting non-native Phragmites rely heavily on accurately mapping invaded areas. Historically, mapping has been done through walking the perimeter of a stand with a Global Positioning System (GPS) unit, using satellite imagery, or through the use of aerial photography from manned aircraft. These methods are time consuming, are expensive, can have an inadequate resolution, and in some cases are prone to human error. In this work, an Unmanned Aerial System (UAS) was used to capture visible imagery to create a basin-wide distribution map of a large wetland along the US Pearl River delta in southeastern Louisiana. The imagery was collected in the summer and individual images were mosaicked to create a larger map. We then evaluated the use of texture analysis on the mosaics to automatically map the invasive. Specifically, Gabor filters, grey level co-occurrence matrices, segmentation-based fractal texture analysis, and wavelet-based texture analysis were compared for mapping the Phragmites. Our experimental results, conducted using the imagery we collected over four study areas (approximately 2250 ha) along the US Pearl River delta, indicate the proposed texture-based approach yields an average accuracy of 85%, an average kappa accuracy of 70%. These maps have shown to be very useful for resource managers to hasten the eradication efforts of Phragmites.

Visible and thermal images acquired from drones (unoccupied aircraft systems) have substantially improved animal monitoring. Combining complementary information from both image types provides a powerful approach for automating detection and classification of multiple animal species to augment drone surveys. We compared eight image fusion methods using thermal and visible drone images combined with two supervised deep learning models, to evaluate the detection and classification of white-tailed deer (Odocoileus virginianus), domestic cow (Bos taurus), and domestic horse (Equus caballus). We classified visible and thermal images separately and compared them with the results of image fusion. Fused images provided minimal improvement for cows and horses compared to visible images alone, likely because the size, shape, and color of these species made them conspicuous against the background. For white-tailed deer, which were typically cryptic against their backgrounds and often in shadows in visible images, the added information from thermal images improved detection and classification in fusion methods from 15 to 85%. Our results suggest that image fusion is ideal for surveying animals inconspicuous from their backgrounds, and our approach uses few image pairs to train compared to typical machine-learning methods. We discuss computational and field considerations to improve drone surveys using our fusion approach.

In recent years, small unmanned aircraft systems (sUAS) have been used widely to monitor animals because of their customizability, ease of operating, ability to access difficult to navigate places, and potential to minimize disturbance to animals. Automatic identification and classification of animals through images acquired using a sUAS may solve critical problems such as monitoring large areas with high vehicle traffic for animals to prevent collisions, such as animal-aircraft collisions on airports. In this research we demonstrate automated identification of four animal species using deep learning animal classification models trained on sUAS collected images. We used a sUAS mounted with visible spectrum cameras to capture 1288 images of four different animal species: cattle (Bos taurus), horses (Equus caballus), Canada Geese (Branta canadensis), and white-tailed deer (Odocoileus virginianus). We chose these animals because they were readily accessible and white-tailed deer and Canada Geese are considered aviation hazards, as well as being easily identifiable within aerial imagery. A four-class classification problem involving these species was developed from the acquired data using deep learning neural networks. We studied the performance of two deep neural network models, convolutional neural networks (CNN) and deep residual networks (ResNet). Results indicate that the ResNet model with 18 layers, ResNet 18, may be an effective algorithm at classifying between animals while using a relatively small number of training samples. The best ResNet architecture produced a 99.18% overall accuracy (OA) in animal identification and a Kappa statistic of 0.98. The highest OA and Kappa produced by CNN were 84.55% and 0.79 respectively. These findings suggest that ResNet is effective at distinguishing among the four species tested and shows promise for classifying larger datasets of more diverse animals.

Wildfires can be beneficial for native vegetation. However, wildfires can impact property values, human safety, and ecosystem function. Resource managers require safe, easy to use, timely, and cost-effective methods for quantifying wildfire damage and regeneration. In this work, we demonstrate an approach using an unmanned aerial system (UAS) equipped with a MicaSense RedEdge multispectral sensor to classify and estimate wildfire damage in a coastal marsh. We collected approximately 7.2 km² of five-band multispectral imagery after a wildfire event in February 2016, which was used to create a photogrammetry-based digital surface model (DSM) and orthomosaic for object-based classification analysis. Airborne light detection and ranging data were used to validate the accuracy of the DSM. Four-band airborne imagery from pre- and post-fire were used to estimate pre-fire health, post-fire damage, and track the vegetation recovery process. Immediate and long-term post-fire classifications, area, and volume of burned regions were produced to track the revegetation progress. The UAS-based classification produced from normalized difference vegetation index and DSM was compared to the Landsat-based Burned Area Reflectance Classification. Experimental results show the potential of using UAS and the presented approach compared to satellite-based mapping in terms of classification accuracies, turnaround time, and spatial and temporal resolutions.