Far-UVC 222 nm is a promising adjunctive disinfection technology for occupied healthcare environments, though antimicrobial efficacy varies significantly across pathogen types due to fundamental differences in microbial biology. This review synthesizes evidence on microbiological determinants of far-UVC resistance, examining cell envelope structure, biofilm formation, DNA repair capacity, and antioxidant defenses. A clear resistance hierarchy emerges. Enveloped viruses lacking enzymatic repair systems are highly vulnerable, requiring fluences below 3 mJ/cm². Gram-negative bacteria are readily inactivated through membrane disruption and reactive oxygen species accumulation. Gram-positive bacteria demonstrate higher resistance via thick peptidoglycan barriers, DNA repair mechanisms, and redundant antioxidant systems. Biofilm-embedded cells show 10–1000-fold increased tolerance due to protective extracellular matrices, stress-response gene upregulation, and microenvironmental heterogeneity. Clostridioides difficile spores exhibit extreme resistance through multilaminar protective coats and metabolic dormancy, requiring impractical doses exceeding 1000 mJ/cm². Field studies in real-world polymicrobial biofilm communities demonstrate substantially lower efficacy than laboratory predictions, typically achieving only 55–81% bioburden reductions. Understanding these pathogen-specific resistance mechanisms is essential for the rational deployment of far-UVC as an adjunctive infection prevention intervention in healthcare settings.

Septika Prismasari, Kyuseok Kim, Hye Young Mun, and Jung Yun Kang. J Dent Hyg Sci 2024;24:22-8. https://doi.org/10.17135/jdhs.2024.24.1.22

Background/Objectives: Allomyrinasin is a cationic antimicrobial peptide derived from Allomyrina dichotoma larvae with known antibacterial and anti-inflammatory properties; however, its effects on migration-related mechanisms in oral squamous cell carcinoma (OSCC) remain poorly understood. This study investigated the anti-migratory potential of allomyrinasin in OSCC cells, focusing on Na⁺/HCO₃⁻ cotransporter (NBC) activity as a key migratory module. Methods: NBC activity was assessed in YD-38 OSCC cells treated with allomyrinasin. Cell migration was evaluated by wound healing and Transwell assays, and MMP expression. Intracellular reactive oxygen species (ROS), apoptosis-related markers, and lamin A/C expression were analyzed using fluorescence-based assays and gene expression analysis. Results: Allomyrinasin inhibited NBC activity and suppressed cell migration without substantial loss of cell viability. MMP-13 was selectively downregulated among the tested MMPs. Lamin A/C expression was markedly upregulated, suggesting enhanced nuclear stiffness that may restrict confined cell migration. Intracellular ROS levels were elevated, and apoptotic progression was confirmed by increased Annexin V/PI positivity along with downregulation of B-cell lymphoma 2 (BCL2) and upregulation of BCL-2–associated X genes (BAX), through a p53-independent pathway consistent with the TP53-deleted status of YD-38 cells. Conclusions: Allomyrinasin suppresses OSCC cell migration by targeting NBC activity as a key component of the migratory machinery, accompanied by oxidative stress induction and pro-apoptotic signaling. These findings identify allomyrinasin as a potential anti-migratory therapeutic candidate and highlight NBC activity as a promising target for attenuating cancer metastasis.

Objective: Dental instruments are often designed for single use; however, some dental clinics resort to reusing instruments, including saliva ejectors. In this study, we aimed to investigate and compare various disinfection and sterilization methods for contaminated disposable salivary ejectors. Methods: Saliva ejectors contaminated with Streptococcus mutans were divided into f ive groups: negative control, positive control, cleaning, disinfection, and sterilization. Surface contamination and microbial culture tests were conducted using the adenosine triphosphate (ATP) test and colony-forming units (CFUs) counting, respectively. One-way ANOVA was used to assess the differences among groups. Results: There were no significant differences among the cleaning, disinfection, and sterilization groups based on immediate ATP test. However, CFU counts were significantly higher in the cleaning and disinfection groups compared to negative control group, while no microbial growth was observed in the sterilization group. Conclusions: This study demonstrated that saliva ejectors harbor bacteria capable of growth, even after cleaning with a toothbrush or disinfectant. Autoclaving proved to be the most effective sterilization method, eliminating microbial growth, and allowing for microbiological reuse. Future studies involving diverse microorganisms and different types of contaminants are recommended.

Objectives: This study aims to conduct a bibliometric analysis of dental hygiene research from 1980 to 2024, evaluating publication trends, prolific authors, influential journals and emerging research themes. Methods: Data were extracted from the Web of Science Core Collection using the keyword ‘dental hygien*’ up to December 26, 2024. A total of 3,055 records were screened, of which 2,315 met the inclusion criteria. Bibliometric indicators, including publication trends, global collaborations, citation analysis, author productivity, journal impact and keyword co‐occurrence were analyzed using VOSviewer and CiteSpace software. Results: The analysis identified publication trends, citation counts and keywords with the strongest citation bursts in dental hygiene research from 1980 to 2024, showing a significant increase in research output since the 2000s. This was followed by an analysis of the distribution of countries and organizations, along with an examination of global and institutional collaboration networks. Additionally, the global distribution of journals and authors was analyzed, and their collaborative networks were explored. Citation analysis of authors, references and journals highlighted influential contributors and key works in the field. Finally, keyword analysis revealed five primary research clusters: Education, Prevention, Occupational health, Public health and Oral health in the elderly. Emerging trends suggest growing interest in public health interventions, technology‐mediated care and interdisciplinary approaches to oral health. Conclusions: Dental hygiene research has evolved significantly, emphasizing its growing importance in healthcare. Key contributors, influential journals and global collaborations have played a pivotal role in advancing the field. This bibliometric analysis offers meaningful insights into the trends, key contributors and emerging areas of dental hygiene research. The findings from this analysis can serve as a valuable resource for researchers seeking advanced knowledge and applications in the field of dental hygiene.