Insufficient surveillance and diagnosis result in a minimum of 150 million cases of serious fungal infections reported annually. The WHO has compiled a list of priority pathogens to encourage research and investment in fungal infections and antifungal resistance in late 2022. Among these, Candida albicans is classified as a critical pathogen. Fluconazole is widely recognized as an effective medication for the treatment and prevention of both mucosal and invasive candidiasis. Molecularly imprinted polymer (MIP) could enhance separation selectivity in fluconazole bioanalysis. The objective of this research is to develop an MIP for fluconazole by analyzing interactions identified in prior research and incorporating established improvements for MIP synthesis that cannot be observed through laboratory experimentation. Based on binding affinity, intermolecular hydrogen bonds, complexation energy, and thermodynamic characteristics, 2-acrylamido-1-ethanesulfonic acid was chosen as the optimal monomer. The HOMO–LUMO investigation revealed the localization of the orbitals from the guest to the host. The FMO study indicated that chloroform was the most suitable solvent for complex formation. The QTAIM, NBO, and NCI-RDG analyses identified the hydrogen bond formed between the H51 atom of the monomer and the N33 atom of fluconazole, determined to be the most significant hydrogen bond in the host–guest interaction. The interaction energy from multi-monomer interaction showed that a 1 : 6 ratio is the best ratio in forming a pre-polymerization complex between the template and monomer. Based on the findings of this study, it is anticipated that the computational analysis may be utilized for rational design for the enhancement of prior studies and future laboratory investigations.

Invasive fungal infections cause serious illness and death worldwide. Long-term therapeutic and preventative use of antifungal drugs in high-risk patients has caused resistance. Triazole antifungals are widely used to prevent and treat fungal infections, and therapeutic drug monitoring has been suggested to improve outcomes, reduce toxicity, and prevent drug resistance. Common methods used for monitoring triazole antifungal drugs in biological matrices such as blood, serum, and plasma include bioassay and instrumentation methods, especially liquid chromatography. Sample preparation is needed to remove interference from liquid chromatography for reliable results. This paper evaluates the use of liquid chromatography to analyze triazole antifungal agents. We provided various chromatographic techniques combined with different detector types to analyze triazole antifungal drugs in biological matrices. We also compared chromatography systems with different sample preparation methods in order to select the most suitable analytical method for bioanalysis.

Mitragyna speciosa, commonly referred to as kratom, is known for its active compound, mitragynine, which is classified as a new psychoactive substance. The availability of mitragynine standards remains a challenge, highlighting the need for effective and efficient methods for isolating this compound from kratom. This study aimed to computationally design a molecularly imprinted polymer (MIP) for the selective isolation of mitragynine. Computational studies were conducted using the B3LYP def2TZVP method with DFT-D4 dispersion, and the results were verified by a laboratory experiment and a molecular dynamics study. The study revealed that methacrylic acid was the optimal monomer for MIP interactions in methanol. Laboratory experiments, employing the association constant and Job plot methods, confirmed that methanol was the ideal solvent for the pre-polymerization complex, with an equilibrium template-to-monomer ratio of 1:3. Radial distribution function analysis from molecular dynamics simulations further supported that the 1:3 template-to-monomer ratio was optimal, aligning with experimental findings. This study’s findings suggest that computational analysis may be employed for the rational design of improved MIPs and for further laboratory investigation into the selective isolation of mitragynine from plants.

Symbols on drug packaging are vital for providing essential information regarding the usage, dosage, and safety warnings of medications. A lack of understanding of these symbols may lead to improper use of drugs, posing potential health risks. This community service initiative aimed to increase public awareness of these symbols through socialization activities targeted at the visitors of Car Free Day (CFD) Jakarta, a popular public gathering space with diverse individuals. The objective of this initiative was to evaluate the level of public understanding before and after the socialization of the symbols on drug packaging. A total of 60 participants were engaged in identifying the symbols on drug packaging, followed by an educational session explaining the meanings of these symbols. Data was collected using questionnaires and analyzed to determine whether there was a notable improvement in participants' understanding. The results indicated a significant increase in public knowledge following the educational intervention. The findings suggest that socialization efforts on the understanding of packaging symbols can enhance public awareness, potentially leading to safer and more informed medication use. It is recommended that similar socialization activities be conducted in other areas with high foot traffic to expand the reach of this educational effort.

Penggunaan formalin sebagai pengawet makanan dilarang karena bersifat karsinogen. Kondisi lingkungan di Jakarta Utara yang bersuhu tinggi, lembab, dan berlokasi dekat laut seringkali menyebabkan penyalahgunaan formalin sebagai pengawet pada sayur-sayuran untuk meningkatkan waktu penyimpanannya, termasuk dalam bunga kol (Brassica oleracea). Penelitian ini bertujuan untuk mendapatkan metode analisis yang valid untuk penetapan formalin dalam bunga kol yang dijual di 6 kecamatan yang terletak di Jakarta Utara. Metode penelitian meliputi optimasi derivatisasi formalin, ekstraksi formalin dari bunga kol, validasi metode analisis, dan penentuan kandungan formalin dalam bunga kol, Hasil penelitian menunjukan kondisi optimum derivatisasi pada konsentrasi asam kromatropat 100 kali konsentrasi formalin, suhu derivatisasi 60°C selama 15 menit. Ekstraksi formalin dilakukan menggunakan asam fosfat 10%, kemudian sampel didestilasi. Metode memberikan hasil yang valid, pengujian spesifisitas menunjukan tidak terdapat interferensi, metode memberikan hasil yang linear pada rentang konsentrasi 2 - 10 ?g/mL. Batas deteksi dan batas kuantisasi metode berturut-turut sebesar 0,55 ?g/mL dan 1,68 ?g/mL. Metode memberikan hasil yang akurat dengan perolehan kembali sebesar 96,2%; 97,4%; dan 96,9% dan presisi dengan koefisien variasi sebesar 2,1%; 2,0%; dan 1,5% berturut-turut pada konsentrasi 4, 5, dan 6 ?g/mL. Pengujian bunga kol di 30 pasar Jakarta Utara menunjukan rerata kandungan formalin sebesar 32,42 mg per kg sampel. Kata Kunci : Bunga Kol, Formalin, Jakarta Utara, Validasi Metode Analisis