This study consisted of two experiments examining the effects of nitrogen (N) forms on growth, flowering, photosystem II efficiency, tuber development, and nitrogen translocation in Jerusalem artichoke (Helianthus tuberosus L.) clone No. 3 (cv. CN 52867), a high-flowering genotype for edible potted plant production. The first experiment used a completely randomized design with four treatments: N-free control, ammonium (NH₄⁺), nitrate (NO₃⁻), and combined NH₄⁺ + NO₃⁻. Results showed that combined N form significantly improved specific leaf area compared with NO₃⁻, NH₄⁺, and the control. The combined treatment consistently produced the highest relative crop growth rate and nitrogen use efficiency across growth stages. It also increased leaf production, shoot and root biomass, and total dry matter. Flowering performance was enhanced under NO₃⁻ and combined N, with higher flowering percentage and larger flowers, while the combined treatment produced the highest flower number, petal number, and flower biomass. For tuber development, NO₃⁻ increased tuber number, whereas combined N produced larger tubers and the highest tuber fresh and dry weights. All N-supplied treatments improved chlorophyll fluorescence parameters (maximum quantum efficiency of photosystem II; Fv/Fm, electron transport rate; ETR, and photochemical quenching coefficient; qP). The second experiment, ¹⁵N tracer analysis, showed a clear shift in nitrogen allocation during development. Vegetative growth favored allocation to leaves and roots, and flowering promoted redistribution to reproductive organs, and pre-dormancy stages directed nitrogen mainly to newly formed tubers, indicating efficient internal recycling and storage. Overall, nitrogen form strongly influenced growth, reproduction, and sink allocation. Combined NO₃⁻ and NH₄⁺ supply optimized both ornamental quality and tuber yield, while ¹⁵N tracing clarified stage-dependent nitrogen dynamics, providing a physiological basis for improved nitrogen management in Jerusalem artichoke production.