Given the increasing challenges posed by frequent extreme climatic events, understanding the climate–human connection between the climate system and the transitions of ancient civilizations is crucial for addressing future climatic challenges, especially when examining the relationship between the abrupt events of the Holocene and the Neolithic culture development. Compared with the globally recognized “4.2 ka collapse” of ancient cultures, the initial start time and the cultural significance of the 5.5 ka climatic fluctuation are more complex and ambiguous. The Hongshan culture (6.5–5.0 ka) is characterized by a complicated society evident in its grand public architecture and elaborate high-status tombs. However, the driving mechanisms behind cultural changes remain complex and subject to ongoing debate. This paper delves into the role of climatic change in Hongshan cultural shifts, presenting an integrated dataset that combines climatic proxy records with archaeological data from the Hongshan culture period. Based on synthesized aeolian, fluvial-lacustrine, loess, and stalagmite deposits, the study indicates a relatively cold and dry climatic fluctuation occurred during ~6.0–5.5 ka, which is widespread in the Horqin dune field and adjacent areas. Combining spatial analysis with ArcGis 10.8 on archaeological sites, we propose that the climatic fluctuation between ~6.0–5.5 ka likely triggered the migration of the Hongshan settlements and adjustment of survival strategies.