Background: High‐intensity focused ultrasound (HIFU) and radiofrequency (RF) are non‐invasive modalities for skin rejuvenation, but their combined effects have not been evaluated.Objective: We evaluated and compared the efficacy of HIFU alone and combined HIFU and bipolar RF using a newly designed probe.Methods: Twenty‐two Korean adults with facial wrinkles and aging underwent treatment on both sides of their face: HIFU‐only on the left and HIFU combined with RF on the right. Skin parameters were measured at different time points to evaluate the improvement in skin rejuvenation.Results: HIFU treatment significantly improved skin parameters, including pore volume and number, skin elasticity, depth of eye wrinkles, degree of sagging in the eye area, nasolabial folds and cheeks, volume of the jawline, skin density, and permittivity. Furthermore, combining bipolar RF with HIFU treatment enhanced efficacy in reducing pore number, improving skin elasticity, diminishing eye wrinkle depth, and increasing skin moisturization. These findings indicate that bipolar RF can synergically improve skin rejuvenation by providing a thermal effect to the upper papillary dermis, which is more superficial than the target area of HIFU.Conclusion: Combining HIFU with bipolar RF synergistically improves skin rejuvenation, including pore reduction, periorbital wrinkle improvement, skin elasticity, and skin moisturization.

Background: Intradermal injection of CPM‐HA20G, a low‐viscoelasticity hyaluronic acid (HA) dermal filler with glycerol, has been shown to be effective for facial rejuvenation in Caucasians, but research in Asians is limited. Aims: This study aimed to evaluate the effectiveness and safety of CPM‐HA20G in enhancing facial skin quality in Korean women using a protocol developed by local aesthetic experts. Patients/Methods: In this 24‐week prospective, single‐arm, open‐label study, 20 women received CPM‐HA20G injections in the immediate subdermal layer on the anterior cheek (1 mL per side; total 2 mL) in three sessions every 4 weeks. Evaluations included biophysical assessments covering the four emergent perceptual categories (EPCs) for skin quality and subjective assessment using the Global Aesthetic Improvement Scale. Results: Significant improvements in skin glow (skin gloss, epidermal hydration), skin firmness (dermal elasticity, dermal hydration), skin surface evenness (average pore volume, pore area, pore density, pore count, maximum pore depth, total pore volume, skin roughness, sebum secretion, and skin depression volume), skin tone evenness (skin color brightness, skin redness), and transepidermal water loss were observed at Week 12 and Week 24 following the first injection with CPM‐HA20G. Most subjects and investigators reported improvements in overall aesthetic appearance with the treatment, with a 100% improvement rating from both groups at Week 12. No serious adverse reactions were observed. Conclusions: Our study provides real‐world insights into the effectiveness and safety of CPM‐HA20G in improving facial skin quality in an Asian population, evaluated through both objective and subjective assessments.

This study aimed to evaluate whether the Synerjet system can maximize the transdermal delivery and skin rejuvenation of nano-NMN. In a 4-week split-face trial (n = 21), this combination demonstrated marked clinical superiority over topical nano-NMN alone (p < 0.001), yielding enhanced improvements in wrinkles (with 170.56% relative improvement in periorbital and 154.45% in nasolabial region compared to the control group), pore volume (176.62%), and deep hydration (188.02%). Regarding dermal integrity, the test group showed a 111.56% superior increment in skin elasticity and a 149.75% more effective optimization of melanin intensity relative to the control. Notably, deep-tissue hydration at a 2.5 mm depth demonstrated a 188.02% higher gain, suggesting that the modality significantly fortifies the skin’s physiological moisture reservoir. The test group exhibited a marked improvement over the control across all cutaneous parameters (p < 0.001). Our findings demonstrate that a new combinatorial approach using EP-assisted microjet of a Synerjet system after cold plasma pretreatment and a nano-NMN 10% ampoule resulted in significantly greater improvements in wrinkles, pores, elasticity, pigmentation, and deep skin hydration compared to topical application alone. Consequently, these results demonstrated that the Synerjet system effectively overcame the inherent limitations of nano-delivery technologies, offering a promising modality for advanced cutaneous rejuvenation and a robust framework for future professional dermatological treatments.

Achieving optimal skin penetration with bioactive cosmetic ingredients, such as epidermal growth factor (EGF), presents ongoing challenges. This study introduces a novel elastosome-based EGF delivery system co-loading dexpanthenol, which achieves superior skin penetration and multifunctional cosmetic efficacy compared with a conventional liposome formulation. The EGF FLEXIR-SOME formulation was characterized to determine its physicochemical properties measured for comparison against a conventional liposome control. Efficacy and safety were confirmed through in vitro and in vivo evaluations, including clinical trials of the formulation and primary skin irritation tests. The formulated EGF FLEXIR-SOME particles exhibited an average diameter of 124.8 nm and a zeta potential of −57.53 mV, demonstrating enhanced stability and skin penetration relative to the control. The results of clinical trials confirmed significant efficacy in anti-aging, moisture, skin barrier improvement, and hyperpigmentation reduction. Additionally, primary skin irritation tests classified the product as a non-irritant. In conclusion, an elastosome-based EGF formulation significantly enhances skin penetration and bioavailability. The formulation effectively improves skin elasticity, hydration, and barrier function while simultaneously reducing visible signs of aging and pigmentation. This study successfully developed an innovative formulation utilizing elastosome technology, maximizing the transdermal efficiency and stability of EGF, thereby offering a novel strategy for functional cosmeceutical development.

This study aimed to evaluate whether the Synerjet system can maximize the trans-dermal delivery and skin rejuvenation of nano-NMN. In a 4-week split-face trial (n=21), this combination demonstrated marked clinical superiority over topical nano-NMN alone (p < 0.001), yielding enhanced improvements in wrinkles (170.56% in periorbital and 154.45% in nasolabial, respectively), pore volume (176.62%), and deep hydration (188.02%). Regarding dermal integrity, the test group showed a 111.56% superior increment in skin elasticity and a 149.75% more effective optimization of melanin intensity. Notably, deep-tissue hydration at a 2.5 mm depth demonstrated a 188.02% higher gain, suggesting that the modality significantly fortifies the skin’s physiological moisture reservoir. The test group exhibited a marked improvement over the control across all cutaneous parameters (p < 0.001). Our findings demonstrate that a new combinatorial approach using EP-assisted microjet of Synerjet system after cold plasma pretreatment and a nano-NMN 10% ampoule resulted in significantly greater improvements in wrinkles, pores, elasticity, pigmentation, and deep skin hydration compared to topical application alone. Consequently, these results demonstrated that Synerjet system effectively overcome the inherent lim-itations of nano-delivery technologies, offering a promising modality for advanced cutaneous rejuvenation and a robust framework for future professional dermatological treatments.

Sponge spicules have emerged as promising biomaterial scaffolds due to their biocompatibility and unique structural properties; however, achieving stable and bioactive functionalization remains a key challenge. The tripeptide GHK is known to promote collagen synthesis and wound repair, yet its therapeutic efficacy is often limited by rapid diffusion and instability. Here, we report ALTUM, a thiol-functionalized sponge spicule composite in which GHK is covalently conjugated via disulfide linkage to enable controlled and redox-responsive peptide delivery. ALTUM exhibited sustained GHK retention under physiological and storage conditions, while exposure to reduced glutathione (GSH) selectively accelerated peptide release through disulfide bond cleavage. This dual release behavior—long-term stability combined with reduction-triggered activation—distinguishes ALTUM from conventional delivery systems. The composite also demonstrated structural stability under thermal, cyclic, and photostability conditions. In an artificial human skin model, ALTUM enhanced dermal penetration of GHK and significantly increased collagen deposition in the dermal layer, demonstrating its capacity to promote collagen production within deeper skin tissue, compared to simple spicule–peptide mixtures. ALTUM was fabricated at an optimized spicule-to-peptide ratio of 3% (w/w), preserving the needle-shaped spicule morphology after surface modification. In vitro, ALTUM exhibited a sustained release profile, with GHK release markedly accelerated in the presence of 10 mM glutathione (GSH) compared with non-reductive conditions, reaching approximately 60% cumulative release over 35 days. In the bioprinted artificial human skin model, ALTUM delivered 9.72 ng/cm² of GHK, more than five-fold higher than the physical mixture of spicules and free GHK (1.9 ng/cm²), and significantly increased type I collagen expression in human dermal fibroblasts. Mechanistically, ALTUM-mediated delivery was associated with increased TGF-β expression and engagement of the SMAD signaling pathway, as indicated by increased phosphorylation of SMAD2/3, consistent with involvement of the TGF-β–SMAD axis in the observed collagen induction. Collectively, these findings establish ALTUM as a structurally stable, redox-responsive dermal delivery platform that enhances collagen synthesis and skin regeneration.