The Nutrient Use Efficiency in intensive agriculture is lower than 50% for macronutrients. This feature results in unsustainable financial and environmental costs. Nanofertilizers are a promising application of nanotechnology in agriculture. The use of nanofertilizers in an efficient and safe manner calls for knowledge about the actual effects of nanoproducts on the plant metabolism and eventually on the carrier release kinetics and nutrient accumulation. Hydroxyapatite (Ca₁₀(PO₄)₆(OH)₂) nanoparticles (nHA) have an interesting potential to be used as nanofertilizers. In this study, the effects of different nHA solutions stabilized with carboxymethylcellulose (CMC) were evaluated on germination, seedling growth, and metabolism of Solanum lycopersicum L., used as model species. Our observations showed that the percentage germination of S. lycopersicum is not influenced by increasing concentrations of nHa, while root elongation is strongly stimulated. Tomato plants grown in hydroponics in the presence of nHA have not suffered phytotoxic effects. We conclude that nHA had nontoxic effects on our model plant and therefore it could be used both as a P supplier and carrier of other elements and molecules.

The use of salt- or macro-sized NPK fertilizers is typically associated with low nutrient use efficiency and water eutrophication. Nanotechnology can overcome such drawbacks, but its practical application on a large scale is limited by (i) high costs and difficult scale-up of nanoparticle synthesis, (ii) questionable advantages over traditional methods, and (iii) health hazards related to nanomaterial introduction in the food stream and the environment. Here, we report on a novel biocompatible and multifunctional P nanofertilizer obtained by self-assembling natural or synthetic humic substances and hydroxyapatite nanoparticles using a simple and straightforward dipping process, exploiting the interaction between the polyphenolic groups of humic substances and the surface of nanohydroxyapatite. Pot tests using the as-prepared materials were performed on Zea mays as a model crop, and the results were compared to those obtained using commercial fused superphosphate and bare nanohydroxyapatites. A significant improvement, in terms of early plant growth, corn productivity, rhizosphere bacteria, and the resistance to NaCl-induced abiotic stresses, was achieved using hydroxyapatite nanoparticles assembled with humic substances. These effects were ascribed to the synergistic co-release of phosphate ions and humic substances, which are two types of plant-beneficial agents for crop nutrition and stimulation, respectively. The release patterns were proven to be tunable with the amount of humic substances adsorbed on the nanoparticles, inducing competition between humic-substance-driven phosphorous dissolution and block of water contact. Such positive effects on plant growth in association with its intrinsic biocompatibility, simple synthesis, and multifunctionality qualify this novel nanofertilizer as a promising material for large-scale use in the agronomic field.

Cystic fibrosis (CF) is a genetic disease affecting today nearly 70,000 patients worldwide and characterized by a hypersecretion of thick mucus difficult to clear arising from the defective CFTR protein. The over-production of the mucus secreted in the lungs, along with its altered composition and consistency, results in airway obstruction that makes the lungs susceptible to recurrent and persistent bacterial infections and endobronchial chronic inflammation, which are considered the primary cause of bronchiectasis, respiratory failure, and consequent death of patients. Despite the difficulty of treating the continuous infections caused by pathogens in CF patients, various strategies focused on the symptomatic therapy have been developed during the last few decades, showing significant positive impact on prognosis. Moreover, nowadays, the discovery of CFTR modulators as well as the development of gene therapy have provided new opportunity to treat CF. However, the lack of effective methods for delivery and especially targeted delivery of therapeutics specifically to lung tissues and cells limits the efficiency of the treatments. Nanomedicine represents an extraordinary opportunity for the improvement of current therapies and for the development of innovative treatment options for CF previously considered hard or impossible to treat. Due to the peculiar environment in which the therapies have to operate characterized by several biological barriers (pulmonary tract, mucus, epithelia, bacterial biofilm) the use of nanotechnologies to improve and enhance drug delivery or gene therapies is an extremely promising way to be pursued. The aim of this review is to revise the currently used treatments and to outline the most recent progresses about the use of nanotechnology for the management of CF.