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Monica Hȃrţa
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Life Sciences Institute Regele Mihai I, 3-5 Mănăștur, 400372, Cluj-Napoca

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Journal article
Published: 29 September 2020 in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
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Streptocarpus x hybridus is a very popular houseplant with colourful flowers arousing thus the interest of plant breeders. Its potential value is even higher since different chemical compounds could be identified and used for food and pharmaceutical industries. Therefore, the objectives of this study were: to determine genetic relationships between twelve F1 Streptocarpus hybrids and their genitors; to determine the total phenolic and flavonoid content; to evaluate the antioxidant activity of the analysed individuals. Although 12 out of the 24 RAPD primers detected polymorphism among the individuals, the UPGMA dendrogram did not show a very clear grouping pattern for flower colour. In contrast, the phytochemical dendrogram shed more light on the dissimilarities among Streptocarpus flowers in terms of their polyphenolic content. The total phenolic and flavonoid content of the flower extracts determined by the Folin-Ciocalteau and aluminium chloride colorimetric methods ranged from 680.02 to 2360.57 mg of GAE/100 g FW and from 12.52 to 78.36 mg QE/100 g FW. The radical scavenging activity against ABTS ranged from 27.17 to 130.35 µM Trolox/g FW. Due to its ornamental value, H12 was screened by HPLC-PDA-ESI-MS to identify and quantify the chemical compounds involved in flower colour. Among the six compounds that were identified and quantified (i.e. cyanidin-3-O-galactoside, cyanidin-3-O-xyloside, cyanidin-3-O-glucoside, cyanidin-3-O-arabinoside, peonidin-3-O-glucoside and apigenin-6-C-glucoside), isovitexin reached the highest concentration 4183 µg rutin/100 g FW. These preliminary results, showing high total polyphenolic content and ABTS antioxidant activity, suggest that Streptocarpus flowers could be a valuable source of antioxidants.

ACS Style

Monica Hȃrţa; Doru Pamfil; Orsolya Borsai; Rodica Pop; Doina Clapa; Zoriţa Diaconeasa; Cristian R. Sisea. Molecular and phytochemical characterization of F1 Streptocarpus hybrids and antioxidant potential of their flower extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2020, 48, 1341 -1356.

AMA Style

Monica Hȃrţa, Doru Pamfil, Orsolya Borsai, Rodica Pop, Doina Clapa, Zoriţa Diaconeasa, Cristian R. Sisea. Molecular and phytochemical characterization of F1 Streptocarpus hybrids and antioxidant potential of their flower extracts. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020; 48 (3):1341-1356.

Chicago/Turabian Style

Monica Hȃrţa; Doru Pamfil; Orsolya Borsai; Rodica Pop; Doina Clapa; Zoriţa Diaconeasa; Cristian R. Sisea. 2020. "Molecular and phytochemical characterization of F1 Streptocarpus hybrids and antioxidant potential of their flower extracts." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 48, no. 3: 1341-1356.

Journal article
Published: 22 May 2020 in Plants
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An efficient micropropagation protocol for Rheum rhabarbarum L. was developed in this study. The in vitro rhubarb plants obtained in the multiplication stage (proliferation rate: 5.0 ± 0.5) were rooted in vitro (96% rooting percentage) and acclimatized ex vitro in floating perlite, with 90% acclimatization percentage. To assess the genetic fidelity between the mother plant and in vitro propagated plants, sequence-related amplified polymorphism (SRAP) markers were used. All banding profiles from the micropropagated plants were monomorphic and similar to those of the mother plant indicating 100% similarity. Regarding the polyphenolic profile, gallic, protocatechuic, p-hydroxybenzoic, vanillic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid were present in different amounts (2.3–2690.3 μg g−1 dry plant), according to the extracted matrix. Aglicons and glycosides of different classes of flavonoids were also identified. The rhizome extracts (both from in vitro and field grown plants) contained resveratrol, a stilbene compound with high antioxidant properties, ranging between 229.4 to 371.7 μg g−1 plant. Our results suggest that in vitro propagation of Rheum rhabarbarum L. represents a reliable alternative to obtain a large number of true-to-type planting material with high bioactive compound content of this valuable nutritional and medicinal species.

ACS Style

Doina Clapa; Orsolya Borsai; Monica Hârța; Victoriţa Bonta; Katalin Szabo; Vasile Coman; Otilia Bobiș. Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L. Plants 2020, 9, 656 .

AMA Style

Doina Clapa, Orsolya Borsai, Monica Hârța, Victoriţa Bonta, Katalin Szabo, Vasile Coman, Otilia Bobiș. Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L. Plants. 2020; 9 (5):656.

Chicago/Turabian Style

Doina Clapa; Orsolya Borsai; Monica Hârța; Victoriţa Bonta; Katalin Szabo; Vasile Coman; Otilia Bobiș. 2020. "Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L." Plants 9, no. 5: 656.

Journal article
Published: 28 January 2020 in Plants
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The genetic relationship among three Streptocarpus parents and twelve F1 hybrids was assessed using sequence-related amplified polymorphism (SRAP) molecular markers and Fourier-transform infrared (FT-IR) spectroscopy. Both methods were able to discriminate F1 hybrids and parents as revealed by cluster analysis. For hybrid identification, the type III SRAP marker was the most effective due to the presence of male-specific bands in the hybrids. Different behaviors in the biochemical variability of DNA samples have been observed by FT-IR spectral analysis, which might be attributed to the inherent nature of the genomic DNA from parents and their F1 progenies. Mantel test was also carried out to compare morphological, SRAP, and FT-IR results based on genetic distances. The highest correlation coefficient was found between morphological and SRAP marker distances (R = 0.607; p ≤ 0.022). A lower correlation was observed between the morphological and FT-IR distance matrix (R = 0.231; p ≤0.008). Moreover, a positive correlation was found between the distances generated with SRAP and FT-IR analyses (R = 0.026) but was not statistically significant. These findings show that both SRAP and FT-IR techniques combined with morphological descriptions can be used effectively for nonconventional breeding programs for Streptocarpus to obtain new and valuable varieties.

ACS Style

Monica Hârţa; Orsolya Borsai; Cristina M. Muntean; Nicoleta E. Dina; Alexandra Fǎlǎmaş; Loredana Elena Olar; Katalin Szabo; Doru Pamfil; Răzvan Ştefan. Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy. Plants 2020, 9, 160 .

AMA Style

Monica Hârţa, Orsolya Borsai, Cristina M. Muntean, Nicoleta E. Dina, Alexandra Fǎlǎmaş, Loredana Elena Olar, Katalin Szabo, Doru Pamfil, Răzvan Ştefan. Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy. Plants. 2020; 9 (2):160.

Chicago/Turabian Style

Monica Hârţa; Orsolya Borsai; Cristina M. Muntean; Nicoleta E. Dina; Alexandra Fǎlǎmaş; Loredana Elena Olar; Katalin Szabo; Doru Pamfil; Răzvan Ştefan. 2020. "Assessment of Genetic Relationships between Streptocarpus x hybridus V. Parents and F1 Progenies Using SRAP Markers and FT-IR Spectroscopy." Plants 9, no. 2: 160.