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Mrs. Otilia Bobis
Life Science Institute, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania

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0 Chromatographic Analysis
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Journal article
Published: 22 July 2021 in Molecules
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Bioactive molecules from the class of polyphenols are secondary metabolites from plants. They are present in honey from nectar and pollen of flowers from where honeybees collect the “raw material” to produce honey. Robinia pseudoacacia and Helianthus annuus are important sources of nectar for production of two monofloral honeys with specific characteristics and important biological activity. A high-performance liquid chromatography–electro spray ionization–mass spectrometry (HPLC–ESI–MS) separation method was used to determine polyphenolic profile from the two types of Romanian unifloral honeys. Robinia and Helianthus honey showed a common flavonoid profile, where pinobanksin (1.61 and 1.94 mg/kg), pinocembrin (0.97 and 1.78 mg/kg) and chrysin (0.96 and 1.08 mg/kg) were identified in both honey types; a characteristic flavonoid profile in which acacetin (1.20 mg/kg), specific only for Robinia honey, was shown; and quercetin (1.85 mg/kg), luteolin (21.03 mg/kg), kaempferol (0.96 mg/kg) and galangin (1.89 mg/kg), specific for Helianthus honey, were shown. In addition, different phenolic acids were found in Robinia and Helianthus honey, while abscisic acid was found only in Robinia honey. Abscisic acid was correlated with geographical location; the samples collected from the south part of Romania had higher amounts, due to climatic conditions. Acacetin was proposed as a biochemical marker for Romanian Robinia honey and quercetin for Helianthus honey.

ACS Style

Otilia Bobiş; Victoriţa Bonta; Mihaiela Cornea-Cipcigan; Gulzar Nayik; Daniel Dezmirean. Bioactive Molecules for Discriminating Robinia and Helianthus Honey: High-Performance Liquid Chromatography–Electron Spray Ionization–Mass Spectrometry Polyphenolic Profile and Physicochemical Determinations. Molecules 2021, 26, 4433 .

AMA Style

Otilia Bobiş, Victoriţa Bonta, Mihaiela Cornea-Cipcigan, Gulzar Nayik, Daniel Dezmirean. Bioactive Molecules for Discriminating Robinia and Helianthus Honey: High-Performance Liquid Chromatography–Electron Spray Ionization–Mass Spectrometry Polyphenolic Profile and Physicochemical Determinations. Molecules. 2021; 26 (15):4433.

Chicago/Turabian Style

Otilia Bobiş; Victoriţa Bonta; Mihaiela Cornea-Cipcigan; Gulzar Nayik; Daniel Dezmirean. 2021. "Bioactive Molecules for Discriminating Robinia and Helianthus Honey: High-Performance Liquid Chromatography–Electron Spray Ionization–Mass Spectrometry Polyphenolic Profile and Physicochemical Determinations." Molecules 26, no. 15: 4433.

Journal article
Published: 23 June 2021 in Applied Sciences
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This study aimed to characterize raw honeys from different geographical origins in Romania, in respect of chemical composition, microbiological examination and evaluate their probiotic potential. The physico-chemical determinations were performed in APHIS-DIA Laboratory, Cluj-Napoca, Romania, using standard validated methods. Bacterial identification was performed for each sample and each colony type using Vitek® 2 Compact 15 system and PCR amplification using 16S rDNA bacterial universal primers (27F, 1492R), species being confirm by sequences analysis. In five raw honey samples, we have identified probiotic bacteria, such as: Bacillus mycoides, Bacillus thuringiensis, Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus velezensis. Generally, all honey samples meet the standard values for chemical composition. However, one sample having 7.44% sucrose was found to have also probiotics bacteria from the genus Bacillus because sucrose is a substrate for probiotics development. In conclusion, the Romanian raw honey can be a potential reservoir of probiotics, which confer a health benefit for consumers.

ACS Style

Claudia Pașca; Liviu Mărghitaș; Ioana Matei; Victorița Bonta; Rodica Mărgăoan; Florina Copaciu; Otilia Bobiș; Maria Campos; Daniel Dezmirean. Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation. Applied Sciences 2021, 11, 5816 .

AMA Style

Claudia Pașca, Liviu Mărghitaș, Ioana Matei, Victorița Bonta, Rodica Mărgăoan, Florina Copaciu, Otilia Bobiș, Maria Campos, Daniel Dezmirean. Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation. Applied Sciences. 2021; 11 (13):5816.

Chicago/Turabian Style

Claudia Pașca; Liviu Mărghitaș; Ioana Matei; Victorița Bonta; Rodica Mărgăoan; Florina Copaciu; Otilia Bobiș; Maria Campos; Daniel Dezmirean. 2021. "Screening of Some Romanian Raw Honeys and Their Probiotic Potential Evaluation." Applied Sciences 11, no. 13: 5816.

Journal article
Published: 18 May 2021 in Processes
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Bee pollen is commonly reputed as a rich source of nutrients, both for bees and humans. Its composition is well balanced and can be taken as a stand-alone food or as supplement, including for the elderly owing its low caloric value. However, storage conditions frequently lead to product degradation, namely due to the high moisture content that enable the proliferation of molds and bacteria. Herein, an infrared (IR)-based technology is proposed as a mean to determine moisture content, setting also a new scalable approach for the development of a drying technology to be used for bee pollen processing, which can be carried out in a short time, without impacting the phenolic and flavonoid content and associated bioactive effects. Proof-of-concept was attained with an IR moisture analyzer, bee pollen samples from Eucalyptus globulus Labill and Salix atrocinerea Brot. being selected as models. Impact of the IR radiation towards the phenolic and flavonoid profiles was screened by HPLC/DAD profiling and radical scavenging ability by the DPPH assay. The IR-based approach shows good reproducibility while simultaneously reducing drying time and energy consumption, thus implying a low environmental impact and being suitable for industrial scale-up once no degradation has been found to occur during the radiation process.

ACS Style

Maria Campos; Christian Frigerio; Otilia Bobiş; Adriana Urcan; Nelson Gomes. Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens. Processes 2021, 9, 890 .

AMA Style

Maria Campos, Christian Frigerio, Otilia Bobiş, Adriana Urcan, Nelson Gomes. Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens. Processes. 2021; 9 (5):890.

Chicago/Turabian Style

Maria Campos; Christian Frigerio; Otilia Bobiş; Adriana Urcan; Nelson Gomes. 2021. "Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens." Processes 9, no. 5: 890.

Journal article
Published: 09 February 2021 in LWT - Food Science and Technology
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Beebread (BB) is a fermented bee product made from plant pollen, honey and bee saliva. The nutritive and biological compounds of BB vary greatly depending on the diversity of flora and the season of pollen collection by bees. In order to evaluate this valuable bee product potential, thirteen BB samples were analyzed on the nutritive proprieties and biologically active compounds, correlated with their botanical origin. The obtained results shows that BB comes with a high content of proteins with values ranging between 18.96 and 23.11 g/100 g, lipids ranging between 4.89 and 14.74 g/100 g, and free sugars, fructose being present in the highest amount (17.27 g/100 g). Analyzed samples contain all the essential amino acids, methionine being the most abundant, 86.93–12.38 mg/100 g, mono- and polyunsaturated fatty acids, ω-6/ω-3 ratio varying between 0.02 – 1.07. BB samples have also high amount of natural antioxidants, from the class of polyphenols and flavonol glycoside derivative. These findings suggest that BB could be used as a source of potential value-added nutrients and bioactive compounds and contributes to the chemical knowledge of this natural product.

ACS Style

Adriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Otilia Bobiș; Victorița Bonta; Francisc Vasile Dulf; Rodica Mărgăoan; Mihaiela Cornea-Cipcigan; Maria Graça Campos. Botanical origin approach for a better understanding of chemical and nutritional composition of beebread as an important value-added food supplement. LWT - Food Science and Technology 2021, 142, 111068 .

AMA Style

Adriana Cristina Urcan, Adriana Dalila Criste, Daniel Severus Dezmirean, Otilia Bobiș, Victorița Bonta, Francisc Vasile Dulf, Rodica Mărgăoan, Mihaiela Cornea-Cipcigan, Maria Graça Campos. Botanical origin approach for a better understanding of chemical and nutritional composition of beebread as an important value-added food supplement. LWT - Food Science and Technology. 2021; 142 ():111068.

Chicago/Turabian Style

Adriana Cristina Urcan; Adriana Dalila Criste; Daniel Severus Dezmirean; Otilia Bobiș; Victorița Bonta; Francisc Vasile Dulf; Rodica Mărgăoan; Mihaiela Cornea-Cipcigan; Maria Graça Campos. 2021. "Botanical origin approach for a better understanding of chemical and nutritional composition of beebread as an important value-added food supplement." LWT - Food Science and Technology 142, no. : 111068.

Review
Published: 24 December 2020 in Plants
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Propolis is a resinous mixture, made by the honeybees from substances collected from tree or other plant buds, plant exudates, or resins found in the stem, branches, or leaves of different plants. The geographical origin of propolis is given by plant sources from respective areas. Different studies have classified this bee product according to the vegetal material from the same areas. Poplar-type propolis has the widest spread in the world, in the temperate zones from Europe, Asia, or North America. The name is given by the main plant source from where the bees are collecting the resins, although other vegetal sources are present in the mentioned areas. Different Pinus spp., Prunus spp., Acacia spp. and also Betula pendula, Aesculus hippocastanum, and Salix alba are important sources of resins for “poplar-type” propolis. The aim of this review is to identify the vegetal material’s chemical composition and activities of plant resins and balms used by the bees to produce poplar-type propolis and to compare it with the final product from similar geographical regions. The relevance of this review is to find the similarities between the chemical composition and properties of plant sources and propolis. The latest determination methods of bioactive compounds from plants and propolis are also reviewed.

ACS Style

Daniel Severus Dezmirean; Claudia Paşca; Adela Ramona Moise; Otilia Bobiş. Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis. Plants 2020, 10, 22 .

AMA Style

Daniel Severus Dezmirean, Claudia Paşca, Adela Ramona Moise, Otilia Bobiş. Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis. Plants. 2020; 10 (1):22.

Chicago/Turabian Style

Daniel Severus Dezmirean; Claudia Paşca; Adela Ramona Moise; Otilia Bobiş. 2020. "Plant Sources Responsible for the Chemical Composition and Main Bioactive Properties of Poplar-Type Propolis." Plants 10, no. 1: 22.

Chapter
Published: 16 December 2020 in Antioxidants in Fruits: Properties and Health Benefits
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The present chapter describes the botanical origin of different species of Vaccinium, along with the chemical composition and properties of these plants. Cranberries are cultivated on large areas, and the fruits are indispensable in nutrition. They are always present in traditional celebrations in different countries. Due to the fact that the fruits of Vaccinium are boosters of vitamins, minerals, and biologically active compounds, increased attention was given to the bioactive properties related to the phytocompounds. Vitamin C, flavonoids, phenolic acids, proanthocyanins, and carotenoids are the principal substances that exhibit the antioxidant activity, controlling the urinary tract disease, glucose level, heart health, and antibacterial and anticancer diseases. Numerous scientific studies describe in detail the benefits as well as the mechanisms of action on the mentioned diseases. In conclusion, cranberries, from different Vaccinium species, are healthy fruits with high nutritional value and benefic phytochemicals fighting against different diseases.

ACS Style

Otilia Bobis; Gulzar Ahmad Nayik; Javed Ahmad Wagay; Umar Farooq; Aiman Zehra; Vikas Nanda. Cranberry. Antioxidants in Fruits: Properties and Health Benefits 2020, 479 -505.

AMA Style

Otilia Bobis, Gulzar Ahmad Nayik, Javed Ahmad Wagay, Umar Farooq, Aiman Zehra, Vikas Nanda. Cranberry. Antioxidants in Fruits: Properties and Health Benefits. 2020; ():479-505.

Chicago/Turabian Style

Otilia Bobis; Gulzar Ahmad Nayik; Javed Ahmad Wagay; Umar Farooq; Aiman Zehra; Vikas Nanda. 2020. "Cranberry." Antioxidants in Fruits: Properties and Health Benefits , no. : 479-505.

Review
Published: 21 November 2020 in Plants
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Nowadays, propolis is used as a highly valuable product in alternative medicine for improving health or treating a large spectrum of pathologies, an ingredient in pharmaceutical products, and also as a food additive. Different vegetal materials are collected by honeybees and mixed with wax and other own substances in order to obtain the final product, called propolis. It is known as the bee product with the widest chemical composition due to the raw material collected by the bees. Different types are known worldwide: green Brazilian propolis (having Baccharis dracunculifolia as the major plant source), red Brazilian propolis (from Dalbergia ecastophyllum), European propolis (Populus nigra L.), Russian propolis (Betula verrucosa Ehrh), Cuban and Venezuelan red propolis (Clusia spp.), etc. An impressive number of scientific papers already demonstrate the pharmacological potential of different types of propolis, the most important activities being the antimicrobial, anti-inflammatory, antitumor, immunomodulatory, and antioxidant activities. However, the bioactive compounds responsible for each activity have not been fully elucidated. This review aims to collect important data about the chemical composition and bioactive properties of the vegetal sources and to compare with the chemical composition of respective propolis types, in order to determine the connection between the floral source and the propolis properties.

ACS Style

Adela Ramona Moise; Otilia Bobiş. Baccharis dracunculifolia and Dalbergia ecastophyllum, Main Plant Sources for Bioactive Properties in Green and Red Brazilian Propolis. Plants 2020, 9, 1619 .

AMA Style

Adela Ramona Moise, Otilia Bobiş. Baccharis dracunculifolia and Dalbergia ecastophyllum, Main Plant Sources for Bioactive Properties in Green and Red Brazilian Propolis. Plants. 2020; 9 (11):1619.

Chicago/Turabian Style

Adela Ramona Moise; Otilia Bobiş. 2020. "Baccharis dracunculifolia and Dalbergia ecastophyllum, Main Plant Sources for Bioactive Properties in Green and Red Brazilian Propolis." Plants 9, no. 11: 1619.

Journal article
Published: 22 May 2020 in Plants
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An efficient micropropagation protocol for Rheum rhabarbarum L. was developed in this study. The in vitro rhubarb plants obtained in the multiplication stage (proliferation rate: 5.0 ± 0.5) were rooted in vitro (96% rooting percentage) and acclimatized ex vitro in floating perlite, with 90% acclimatization percentage. To assess the genetic fidelity between the mother plant and in vitro propagated plants, sequence-related amplified polymorphism (SRAP) markers were used. All banding profiles from the micropropagated plants were monomorphic and similar to those of the mother plant indicating 100% similarity. Regarding the polyphenolic profile, gallic, protocatechuic, p-hydroxybenzoic, vanillic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid were present in different amounts (2.3–2690.3 μg g−1 dry plant), according to the extracted matrix. Aglicons and glycosides of different classes of flavonoids were also identified. The rhizome extracts (both from in vitro and field grown plants) contained resveratrol, a stilbene compound with high antioxidant properties, ranging between 229.4 to 371.7 μg g−1 plant. Our results suggest that in vitro propagation of Rheum rhabarbarum L. represents a reliable alternative to obtain a large number of true-to-type planting material with high bioactive compound content of this valuable nutritional and medicinal species.

ACS Style

Doina Clapa; Orsolya Borsai; Monica Hârța; Victoriţa Bonta; Katalin Szabo; Vasile Coman; Otilia Bobiș. Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L. Plants 2020, 9, 656 .

AMA Style

Doina Clapa, Orsolya Borsai, Monica Hârța, Victoriţa Bonta, Katalin Szabo, Vasile Coman, Otilia Bobiș. Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L. Plants. 2020; 9 (5):656.

Chicago/Turabian Style

Doina Clapa; Orsolya Borsai; Monica Hârța; Victoriţa Bonta; Katalin Szabo; Vasile Coman; Otilia Bobiș. 2020. "Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L." Plants 9, no. 5: 656.

Review article
Published: 28 April 2020 in Food Chemistry
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Eucalyptus honey is an important unifloral honey commercialized worldwide and much desired by consumers due to the medicinal properties attributed to it because of the plant from which it is produced. In general, eucalyptus honey has been classified as being rich in pollen grains from the eucalyptus tree as well as having physicochemical characteristics that, in a way, have made it stand out from other honeys. Similar to other types of honey, eucalyptus honey can suffer contaminations and adulterations that compromise its quality, safety and authenticity. Thus, detailed knowledge of the composition and properties of this monofloral honeys is of great importance. With this background, the aim of this review is to present and discuss recent data regarding the physicochemical characteristics, chemical and health-promoting properties of eucalyptus honey as well as microbial contamination, authenticity, processing and adulteration.

ACS Style

Otilia Bobis; Adela Ramona Moise; Isabel Ballesteros; Estefanía Sánchez Reyes; Silvia Sánchez Durán; José Sánchez-Sánchez; Sandra Cruz-Quintana; Francesca Giampieri; Maurizio Battino; José M. Alvarez-Suarez. Eucalyptus honey: Quality parameters, chemical composition and health-promoting properties. Food Chemistry 2020, 325, 126870 .

AMA Style

Otilia Bobis, Adela Ramona Moise, Isabel Ballesteros, Estefanía Sánchez Reyes, Silvia Sánchez Durán, José Sánchez-Sánchez, Sandra Cruz-Quintana, Francesca Giampieri, Maurizio Battino, José M. Alvarez-Suarez. Eucalyptus honey: Quality parameters, chemical composition and health-promoting properties. Food Chemistry. 2020; 325 ():126870.

Chicago/Turabian Style

Otilia Bobis; Adela Ramona Moise; Isabel Ballesteros; Estefanía Sánchez Reyes; Silvia Sánchez Durán; José Sánchez-Sánchez; Sandra Cruz-Quintana; Francesca Giampieri; Maurizio Battino; José M. Alvarez-Suarez. 2020. "Eucalyptus honey: Quality parameters, chemical composition and health-promoting properties." Food Chemistry 325, no. : 126870.

Journal article
Published: 27 September 2019 in Foods
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Honey composition and color depend greatly on the botanical and geographical origin. Water content, water activity and color of 50 declared acacia samples, collected from three different geographical zones of Romania, together with chromatographic determination of sugar spectrum were analyzed. A number of 79 volatile compounds from the classes of: Alcohols, aldehydes, esters, ketones, sulphur compounds, aliphatic hydrocarbons, nitrogen compounds, carboxylic acids, aromatic acids and ethers were identified by solid-phase micro-extraction and gas-chromatography mass spectrometry. The overall volatile profile and sugar spectrum of the investigated honey samples allow the differentiation of geographical origin for the acacia honey samples subjected to analysis. The statistical models of the chromatic determination, physicochemical parameters and volatile profile was optimal to characterize the honey samples and group them into three geographical origins, even they belong to the same botanical origin.

ACS Style

Niculina M. Mădaş; Liviu A. Mărghitaş; Daniel S. Dezmirean; Victorita Bonta; Otilia Bobiş; Marie-Laure Fauconnier; Frédéric Francis; Eric Haubruge; Kim B. Nguyen. Volatile Profile and Physico-Chemical Analysis of Acacia Honey for Geographical Origin and Nutritional Value Determination. Foods 2019, 8, 445 .

AMA Style

Niculina M. Mădaş, Liviu A. Mărghitaş, Daniel S. Dezmirean, Victorita Bonta, Otilia Bobiş, Marie-Laure Fauconnier, Frédéric Francis, Eric Haubruge, Kim B. Nguyen. Volatile Profile and Physico-Chemical Analysis of Acacia Honey for Geographical Origin and Nutritional Value Determination. Foods. 2019; 8 (10):445.

Chicago/Turabian Style

Niculina M. Mădaş; Liviu A. Mărghitaş; Daniel S. Dezmirean; Victorita Bonta; Otilia Bobiş; Marie-Laure Fauconnier; Frédéric Francis; Eric Haubruge; Kim B. Nguyen. 2019. "Volatile Profile and Physico-Chemical Analysis of Acacia Honey for Geographical Origin and Nutritional Value Determination." Foods 8, no. 10: 445.

Review
Published: 26 July 2019 in Food Reviews International
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ACS Style

Mariana Niculina Mădaş; Liviu Alexandru Mărghitaş; Daniel Severus Dezmirean; Otilia Bobiş; Ouissam Abbas; Sabine Danthine; Frédéric Francis; Eric Haubruge; Bach Kim Nguyen. Labeling Regulations and Quality Control of Honey Origin: A Review. Food Reviews International 2019, 36, 215 -240.

AMA Style

Mariana Niculina Mădaş, Liviu Alexandru Mărghitaş, Daniel Severus Dezmirean, Otilia Bobiş, Ouissam Abbas, Sabine Danthine, Frédéric Francis, Eric Haubruge, Bach Kim Nguyen. Labeling Regulations and Quality Control of Honey Origin: A Review. Food Reviews International. 2019; 36 (3):215-240.

Chicago/Turabian Style

Mariana Niculina Mădaş; Liviu Alexandru Mărghitaş; Daniel Severus Dezmirean; Otilia Bobiş; Ouissam Abbas; Sabine Danthine; Frédéric Francis; Eric Haubruge; Bach Kim Nguyen. 2019. "Labeling Regulations and Quality Control of Honey Origin: A Review." Food Reviews International 36, no. 3: 215-240.

Journal article
Published: 16 December 2018 in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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Bee bread is a product of the hive obtained from pollen collected by bees, to which they add honey, digestive enzymes and subsequently is stored in the combs. Increasing evidence suggests bee bread’s potential therapeutic benefits, including antimicrobial properties. Bee bread is characterized by a bactericidal and bacteriostatic activity. The current study was carried out to test the antimicrobial activity of bee bread extracts, in various concentrations, against the bacterial strains: Bacillus cereus, Staphylococcus aureus, Escherichia coli, Salmonella enterica and Pseudomonas aeruginosa. The results of this study indicate that the first two dilutions of bee bread extract, respectively 33% and 16.66%, showed higher antimicrobial activity and the other dilutions had a lower, but visible activity depending on the pathogen on which they are tested. The best antimicrobial activity was manifested on the Staphylococcus aureus strain, where all dilutions had an inhibitory effect both at 8 hours and 12 hours.

ACS Style

Adriana Urcan; Adriana Criste; Daniel Dezmirean; Otilia Bobiș; Liviu Mărghitaș; Rodica Mărgăoan; Alexandra Hrinca. Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2018, 75, 85 -91.

AMA Style

Adriana Urcan, Adriana Criste, Daniel Dezmirean, Otilia Bobiș, Liviu Mărghitaș, Rodica Mărgăoan, Alexandra Hrinca. Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2018; 75 (2):85-91.

Chicago/Turabian Style

Adriana Urcan; Adriana Criste; Daniel Dezmirean; Otilia Bobiș; Liviu Mărghitaș; Rodica Mărgăoan; Alexandra Hrinca. 2018. "Antimicrobial Activity of Bee Bread Extracts Against Different Bacterial Strains." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 75, no. 2: 85-91.

Review
Published: 16 December 2018 in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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Morus spp. is a perennial and woody plant, belonging to Moraceae family, genus Morus and is essential for sericulture. One of the main aims of Global Centre of Excellence for Advance Research in Sericulture and Promotion of Silk Production (GCEARS-PSP) is to create a reserve of Morus spp., to multiply the vegetal material and to provide it to farmers and also to create an important biotechnological support for further scientifically works. As in Romania the intensive plantations are almost non-existent, one of the main objective of the centre is to create a real base for sericulture revival (the existence of nutritional base for silkworms) and to develop the biotechnological scientific component of the centre.The aims of this review are to create a clear overview of moriculture for sericulture and to reveal the biotechnological potential and pharmacological uses of different mulberry parts. In this review the major findings regarding description of Kokuso 21 variety, mulberry micropropagation and bioactive compounds of different vegetal parts from Mulberry spp. are presented, and represent only the first stage of the GCEARS-PSP activity.

ACS Style

Adela Ramona Moise; Liviu Alexandru Marghitas; Otilia Bobis; Florina Maria Copaciu; Daniel Severus Dezmirean. MORUS SPP. Material Conservation and Characterization and its Importance for Romanian Sericulture and GCEARS-PSP Development- A Review. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2018, 75, 57 -63.

AMA Style

Adela Ramona Moise, Liviu Alexandru Marghitas, Otilia Bobis, Florina Maria Copaciu, Daniel Severus Dezmirean. MORUS SPP. Material Conservation and Characterization and its Importance for Romanian Sericulture and GCEARS-PSP Development- A Review. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2018; 75 (2):57-63.

Chicago/Turabian Style

Adela Ramona Moise; Liviu Alexandru Marghitas; Otilia Bobis; Florina Maria Copaciu; Daniel Severus Dezmirean. 2018. "MORUS SPP. Material Conservation and Characterization and its Importance for Romanian Sericulture and GCEARS-PSP Development- A Review." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 75, no. 2: 57-63.

Journal article
Published: 19 May 2018 in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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The Global Centre of Excellence for Advance Research in Sericulture and Promotion of Silk Production (GCEARS-PSP), recognised by ISC since 2014, was created with the main purposes: development of advanced research in sericulture and diagnostic services using modern techniques, achievement of a gene pool of different silkworms races and promotion of silk production. The objective of this study was to characterize the races from the GCEARS-PSP, in order to conserve the most valuable and resistant ones. This characterisation is a part of GCEARS-PSP’s strategy for the revival and development of sericulture and silk production in Romania. The GCEARS-PSP represents an important point of reference for Romanian sericulture. The experimental data described showed a good prospective for a proper functioning of the research centre and the obtained results are promising for the next lines of research.

ACS Style

Daniel Severus Dezmirean; Liviu Alexandru Marghitas; Otilia Bobiş; Adriana Cristina Urcan; Horatiu Dezmirean; Claudia Pasca; Adela Ramona Moise. Multidirectional Activities for Gene Pool Conservation in GCEARS-PSP. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2018, 75, 5 -10.

AMA Style

Daniel Severus Dezmirean, Liviu Alexandru Marghitas, Otilia Bobiş, Adriana Cristina Urcan, Horatiu Dezmirean, Claudia Pasca, Adela Ramona Moise. Multidirectional Activities for Gene Pool Conservation in GCEARS-PSP. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2018; 75 (1):5-10.

Chicago/Turabian Style

Daniel Severus Dezmirean; Liviu Alexandru Marghitas; Otilia Bobiş; Adriana Cristina Urcan; Horatiu Dezmirean; Claudia Pasca; Adela Ramona Moise. 2018. "Multidirectional Activities for Gene Pool Conservation in GCEARS-PSP." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 75, no. 1: 5-10.

Review article
Published: 04 February 2018 in Oxidative Medicine and Cellular Longevity
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Diabetes is a metabolic disorder with multifactorial and heterogeneous etiologies. Two types of diabetes are common among humans: type 1 diabetes that occurs when the immune system attacks and destroys insulin and type 2 diabetes, the most common form, that may be caused by several factors, the most important being lifestyle, but also may be determined by different genes. Honey was used in folk medicine for a long time, but the health benefits were explained in the last decades, when the scientific world was concerned in testing and thus explaining the benefits of honey. Different studies demonstrate the hypoglycemic effect of honey, but the mechanism of this effect remains unclear. This review presents the experimental studies completed in the recent years, which support honey as a novel antidiabetic agent that might be of potential significance for the management of diabetes and its complications and also highlights the potential impacts and future perspectives on the use of honey as an antidiabetic agent.

ACS Style

Otilia Bobiş; Daniel S. Dezmirean; Adela Ramona Moise. Honey and Diabetes: The Importance of Natural Simple Sugars in Diet for Preventing and Treating Different Type of Diabetes. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -12.

AMA Style

Otilia Bobiş, Daniel S. Dezmirean, Adela Ramona Moise. Honey and Diabetes: The Importance of Natural Simple Sugars in Diet for Preventing and Treating Different Type of Diabetes. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-12.

Chicago/Turabian Style

Otilia Bobiş; Daniel S. Dezmirean; Adela Ramona Moise. 2018. "Honey and Diabetes: The Importance of Natural Simple Sugars in Diet for Preventing and Treating Different Type of Diabetes." Oxidative Medicine and Cellular Longevity 2018, no. : 1-12.

Journal article
Published: 26 November 2017 in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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Neonicotinoids are systemic insecticides used in agriculture for seed treatment as well as in the pest control by spraying during plant growing. Also, they are found as an active substance in different phytosanitary products used in horticulture for winter spraying in fruit plantations when fruit trees are in vegetative rest. Taking into account the former studies on this topic, knowing all what is published regarding this important theme, both for agriculture and for beekeeping, the review is very important for all interested parts. In the present review many of the questions raised by the beekeepers and the scientists from agriculture receive an answer, together with described methods for identifying and quantifying this class of still used pesticide.

ACS Style

Erzsébet Timea Domokos; Liviu Alexandru Marghitas; Daniel Severus Dezmirean; Otilia Bobis. Is Beekeeping Affected by the Use of Neonicotinoids in Agriculture? Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2017, 74, 84 -91.

AMA Style

Erzsébet Timea Domokos, Liviu Alexandru Marghitas, Daniel Severus Dezmirean, Otilia Bobis. Is Beekeeping Affected by the Use of Neonicotinoids in Agriculture? Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2017; 74 (2):84-91.

Chicago/Turabian Style

Erzsébet Timea Domokos; Liviu Alexandru Marghitas; Daniel Severus Dezmirean; Otilia Bobis. 2017. "Is Beekeeping Affected by the Use of Neonicotinoids in Agriculture?" Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 74, no. 2: 84-91.

Journal article
Published: 26 November 2017 in Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies
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The European plum (Prunus domestica L.) is one of the most important cultivated species among fruit trees in the world, and the dominant in Romania. Regarding the development of new plum cultivars derived from Prunus domestica, the following characteristics are taken into consideration most of the time: high yield performance, good quality fruits and last but not least, the resistance to PPV. “HoneySweet” is a transgenic clone of Prunus domestica transformed with Plum pox virus coat protein gene making it highly resistant to the virus. This study presents preliminary results on the chemical composition of HoneySweet transgenic plum and two conventional ones: Reine Claude d’Althan and Stanley. The most important nutritional factors were determined. HoneySweet had balanced individual carbohydrates content but lower soluble solids in comparison with the two conventional cultivars. Total lipids content in HoneySweet fruits was similar to Stanley, higher than in Reine Claude d’Althan. HoneySweet is distinguished by the highest total protein content. Similar amounts of magnesium and calcium were recorded in the three varieties. Overall, our preliminary results revealed that the quality and nutritional value of “Honey Sweet” genetic engineered plum is in the range of other valuable plum cultivars.

ACS Style

Otilia Bobiş; Ioan Zagrai; Victoriţa Bonta; Luminiţa Zagrai; Liviu A. Mărghitaş; Daniel S. Dezmirean; Claudia Paşca; Adriana Urcan. Comparative Studies on Chemical Composition of Two Conventional Bred and One Genetically Engineered Plum-Fruits. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 2017, 74, 149 -156.

AMA Style

Otilia Bobiş, Ioan Zagrai, Victoriţa Bonta, Luminiţa Zagrai, Liviu A. Mărghitaş, Daniel S. Dezmirean, Claudia Paşca, Adriana Urcan. Comparative Studies on Chemical Composition of Two Conventional Bred and One Genetically Engineered Plum-Fruits. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies. 2017; 74 (2):149-156.

Chicago/Turabian Style

Otilia Bobiş; Ioan Zagrai; Victoriţa Bonta; Luminiţa Zagrai; Liviu A. Mărghitaş; Daniel S. Dezmirean; Claudia Paşca; Adriana Urcan. 2017. "Comparative Studies on Chemical Composition of Two Conventional Bred and One Genetically Engineered Plum-Fruits." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 74, no. 2: 149-156.

Hive product science
Published: 25 September 2017 in Journal of Apicultural Research
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Natural honey bee products, especially propolis are well known for their biological properties. However, less well known are the impact of propolis’ single phenolic substances and variation across different landscapes, geographic locations and botanical origins. Here, we determined the chemical composition of propolis samples collected in different geographic areas of Romania with a continental climate. Main groups of polyphenols, as well as individual phenolics (phenolic acids and flavonoids), were identified and quantified. The antimicrobial activity of propolis ethanolic extracts and some of the most important propolis phenolics (caffeic and ferulic acid, pinocembrin, chrysin and galangin) were evaluated against different bacterial species. Analyzing the interaction of bacterial growth inhibition and different chemical parameters, revealed that flavone/flavonol, flavanone/dihydroflavonol, chrysin and galangin are largely responsible for the propolis’ antimicrobial activity against Gram-positive bacteria. Gram-negative bacteria were mostly inhibited by galangin, caffeic acid and naringenin. Chemical composition and antimicrobial activity of the propolis samples might be explained by the botanical sources present in the specific foraging areas. The highest amounts of polyphenols and flavonoids were measured in regions where deciduous forests were dominant to coniferous, or where large areas of willow could be observed.

ACS Style

Daniel S Dezmirean; Liviu A Mărghitaş; Flore Chirilă; Florina Copaciu; Vasile Simonca; Otilia Bobiş; Silvio Erler. Influence of geographic origin, plant source and polyphenolic substances on antimicrobial properties of propolis against human and honey bee pathogens. Journal of Apicultural Research 2017, 56, 588 -597.

AMA Style

Daniel S Dezmirean, Liviu A Mărghitaş, Flore Chirilă, Florina Copaciu, Vasile Simonca, Otilia Bobiş, Silvio Erler. Influence of geographic origin, plant source and polyphenolic substances on antimicrobial properties of propolis against human and honey bee pathogens. Journal of Apicultural Research. 2017; 56 (5):588-597.

Chicago/Turabian Style

Daniel S Dezmirean; Liviu A Mărghitaş; Flore Chirilă; Florina Copaciu; Vasile Simonca; Otilia Bobiş; Silvio Erler. 2017. "Influence of geographic origin, plant source and polyphenolic substances on antimicrobial properties of propolis against human and honey bee pathogens." Journal of Apicultural Research 56, no. 5: 588-597.

Journal article
Published: 04 September 2017 in Molecules
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Bovine mastitis a major disease that is commonly associated with bacterial infection. The common treatment is with antibiotics administered intramammary into infected quarters of the udder. The excessive use of antibiotics leads to multidrug resistance and associated risks for human health. In this context, the search for alternative drugs based on plants has become a priority in livestock medicine. These products have a low manufacturing cost and no reports of antimicrobial resistance to these have been documented. In this context, the main objective of this study was to determine the antimicrobial effect of extracts and products of several indigenous, or acclimatized plants on pathogens isolated from bovine mastitis. A total of eleven plant alcoholic extracts and eight plant-derived products were tested against 32 microorganisms isolated from milk. The obtained results have shown an inhibition of bacterial growth for all tested plants, with better results for Evernia prunastri, Artemisia absinthium, and Lavandula angustifolia. Moreover, E. prunastri, Populus nigra, and L. angustifolia presented small averages of minimum inhibitory and bactericidal concentrations. Among the plant-derived products, three out of eight have shown a strong anti-microbial effect comparable with the effect of florfenicol and enrofloxacin, and better than individual plant extracts possibly due to synergism. These results suggest an important anti-microbial effect of these products on pathogens isolated from bovine mastitis with a possible applicability in this disease.

ACS Style

Claudia Pașca; Liviu Mărghitaș; Daniel Dezmirean; Otilia Bobiș; Victorița Bonta; Flore Chirilă; Ioana Matei; Nicodim Fiț. Medicinal Plants Based Products Tested on Pathogens Isolated from Mastitis Milk. Molecules 2017, 22, 1473 .

AMA Style

Claudia Pașca, Liviu Mărghitaș, Daniel Dezmirean, Otilia Bobiș, Victorița Bonta, Flore Chirilă, Ioana Matei, Nicodim Fiț. Medicinal Plants Based Products Tested on Pathogens Isolated from Mastitis Milk. Molecules. 2017; 22 (9):1473.

Chicago/Turabian Style

Claudia Pașca; Liviu Mărghitaș; Daniel Dezmirean; Otilia Bobiș; Victorița Bonta; Flore Chirilă; Ioana Matei; Nicodim Fiț. 2017. "Medicinal Plants Based Products Tested on Pathogens Isolated from Mastitis Milk." Molecules 22, no. 9: 1473.

Journal article
Published: 26 June 2017 in Modern Applications of Bioequivalence & Bioavailability
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ACS Style

Otilia Bobis. Bee Products and Oxidative Stress: Bioavailability of Their Functional Constituents. Modern Applications of Bioequivalence & Bioavailability 2017, 1, 1 .

AMA Style

Otilia Bobis. Bee Products and Oxidative Stress: Bioavailability of Their Functional Constituents. Modern Applications of Bioequivalence & Bioavailability. 2017; 1 (3):1.

Chicago/Turabian Style

Otilia Bobis. 2017. "Bee Products and Oxidative Stress: Bioavailability of Their Functional Constituents." Modern Applications of Bioequivalence & Bioavailability 1, no. 3: 1.