Heavy-metal (HM) pollution is considered a leading source of environmental contamination. Heavy-metal pollution in ground water poses a serious threat to human health and the aquatic ecosystem. Conventional treatment technologies to remove the pollutants from wastewater are usually costly, time-consuming, environmentally destructive, and mostly inefficient. Phytoremediation is a cost-effective green emerging technology with long-lasting applicability. The selection of plant species is the most significant aspect for successful phytoremediation. Aquatic plants hold steep efficiency for the removal of organic and inorganic pollutants. Water hyacinth (Eichhornia crassipes), water lettuce (Pistia stratiotes) and Duck weed (Lemna minor) along with some other aquatic plants are prominent metal accumulator plants for the remediation of heavy-metal polluted water. The phytoremediation potential of the aquatic plant can be further enhanced by the application of innovative approaches in phytoremediation. A summarizing review regarding the use of aquatic plants in phytoremediation is gathered in order to present the broad applicability of phytoremediation.

Contamination of soil and water with metals and metalloids is one of the most serious problems worldwide due to a lack of a healthy diet and food scarcity. Moreover, the cultivation of oilseed crops such as rapeseed (Brassica napus L.) with tannery wastewater could contain a large amount of toxic heavy metals [e.g., chromium (Cr)], which ultimately reduce its yield and directly influence oilseed quality. To overcome Cr toxicity in B. napus, a pot experiment was conducted to enhance plant growth and biomass by using newly introduced role of micronutrient-amino chelates [Zinc-lysine (Zn-lys)], which was irrigated with different levels [0% (control), 33%, 66%, and 100%] of tannery wastewater. According to the results of present findings, very high content of Cr in the wastewater directly affected plant growth and composition as well as gas exchange parameters, while boosting up the production of reactive oxygen species (ROS) and induced oxidative damage in the roots and leaves of B. napus. However, activities of antioxidants initially increased (33% of wastewater), but further addition of tannery wastewater in the soil caused a decrease in antioxidant enzymes, which also manifested by Zn content, while the conscious addition of wastewater significantly increased Cr content in the roots and shoots of B. napus. To reduce Cr toxicity in B. napus plants, exogenous supplementation of Zn-lys (10 mg/L) plays an effective role in increasing morpho-physiological attributes of B. napus and also reduces the oxidative stress in the roots and leaves of the oilseed crop (B. napus). Enhancement in different growth attributes was directly linked with increased in antioxidative enzymes while decreased uptake and accumulation of Cr content in B. napus when cultivated in wastewater with the application of Zn-lys. Zn-lys, therefore, plays a protective role in reducing the Cr toxicity of B. napus through an increase in plant growth and lowering of Cr uptake in various plant organs. However, further studies at field levels are required to explore the mechanisms of Zn–lys mediated reduction of Cr and possibly other heavy metal toxicity in plants.

Chromium (Cr) is among the most widespread toxic trace elements found in agricultural soils due to various anthropogenic activities. However, the role of micronutrient-amino chelates on reducing Cr toxicity in crop plants was recently introduced. In the current experiment, the exogenous application of micronutrients [iron (Fe)] chelated with amino acid [lysine (lys)] was examined, using an in vivo approach that involved plant growth and biomass, photosynthetic pigments and gaseous exchange parameters, oxidative stress indicators and antioxidant response. The uptake and accumulation of Fe and Cr were determined under different levels of tannery wastewater (33, 66, 100%) used along with the exogenous supplementation of Fe-lys (5 mM) to Spinacia oleracea plants. Results revealed that tannery wastewater in the soil decreased plant growth and growth-related attributes, photosynthetic apparatus and Fe contents in different parts of the plants. In contrast, the addition of different levels of tannery wastewater to the soil significantly increased the contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂) and electrolyte leakage (EL), which induced oxidative damage in the roots and leaves of S. oleracea plants. However, S. oleracea plants increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), which scavenge the over-production of reactive oxygen species (ROS). Cr toxicity can be overcome by the supplementation of Fe-lys, which significantly increased plant growth and biomass, improved photosynthetic machinery and increased the activities of different antioxidative enzymes, even in the plants grown under different levels of tannery wastewater in the soil. Furthermore, the supplementation of Fe-lys increased the contents of essential nutrients (Fe) and decreased the contents of Cr in all plant parts compared to the plants cultivated in tannery wastewater without application of Fe-lys. In conclusion, the application of Fe-lys is an innovative approach to mitigate Cr stress in spinach plants, which not only increased plant growth and biomass but also decreased the Cr contents in different plant organs.