Heavy metal (HM) pollution of soils is a globally important ecological and environmental problem. Previous studies have focused on i) tracking pollution sources in HM-contaminated soils, ii) exploring the adsorption capacity and distribution of HMs, and iii) assessing phyto-uptake of HMs and their ecotoxicity. However, few reviews have systematically summarized HM pollution in soil-plant systems over the past decade. Understanding the mechanisms of interaction between HMs and solid soil components is consequently key to effectively controlling and remediating HM pollution. However, the compositions of solid soil phases are diverse, their structures are complex, and their spatial arrangements are heterogeneous, all leading to the formation of soil micro-domains that exhibit different particle sizes and surface properties. The various soil components and their interactions ultimately control the speciation, transformation, and bioavailability of HMs in soils. Over the past few decades, the extensive application of advanced instrumental techniques and methods has greatly expanded our understanding of the behavior of HMs in organic mineral assemblages. In this review, studies investigating the immobilization of HMs by minerals, organic compounds, microorganisms, and their associated complexes are summarized, with a particular emphasis on the interfacial adsorption and immobilization of HMs. In addition, methods for analyzing the speciation and distribution of HMs in aggregates of natural soils with different particle sizes are also discussed. Moreover, we also review the methods for speciating HMs at mineral-organic micro-scale interfaces. Lastly, developmental prospects for HM research at inorganic-organic interfaces are outlined. In future research, the most advanced methods should be used to characterize the interfaces and in situ characteristics of metals and metal complexes. In particular, the roles and contributions of microorganisms in the immobilization of HMs at complex mineral-organic interfaces require significant further investigation.

Water stress (drought and waterlogging) is severe abiotic stress to plant growth and development. Melatonin, a bioactive plant hormone, has been widely tested in drought situations in diverse plant species, while few studies on the role of melatonin in waterlogging stress conditions have been published. In the current review, we analyze the biostimulatory functions of melatonin on plants under both drought and waterlogging stresses. Melatonin controls the levels of reactive oxygen and nitrogen species and positively changes the molecular defense to improve plant tolerance against water stress. Moreover, the crosstalk of melatonin and other phytohormones is a key element of plant survival under drought stress, while this relationship needs further investigation under waterlogging stress. In this review, we draw the complete story of water stress on both sides—drought and waterlogging—through discussing the previous critical studies under both conditions. Moreover, we suggest several research directions, especially for waterlogging, which remains a big and vague piece of the melatonin and water stress puzzle.

Melatonin (MT) is a pleiotropic molecule with diverse and numerous actions both in plants and animals. In plants, MT acts as an excellent promotor of tolerance against abiotic stress situations such as drought, cold, heat, salinity, and chemical pollutants. In all these situations, MT has a stimulating effect on plants, fomenting many changes in biochemical processes and stress-related gene expression. Melatonin plays vital roles as an antioxidant and can work as a free radical scavenger to protect plants from oxidative stress by stabilization cell redox status; however, MT can alleviate the toxic oxygen and nitrogen species. Beyond this, MT stimulates the antioxidant enzymes and augments antioxidants, as well as activates the ascorbate–glutathione (AsA–GSH) cycle to scavenge excess reactive oxygen species (ROS). In this review, we examine the recent data on the capacity of MT to alleviate the effects of common abiotic soil stressors, such as salinity, alkalinity, acidity, and the presence of heavy metals, reinforcing the general metabolism of plants and counteracting harmful agents. An exhaustive analysis of the latest advances in this regard is presented, and possible future applications of MT are discussed.

Boron (B) is a microelement required in vascular plants at a high concentration that produces excess boron and toxicity in many crops. B stress occurs widely and limits plant growth and crop productivity worldwide. Salicylic acid (SA) is an essential hormone in plants and is a phenolic compound. The goal of this work is to explore the role of SA in the alleviation of excess B (10 mg L⁻¹) in watermelon plants at a morphological and biochemical level. Excess boron altered the nutrient concentrations and caused a significant reduction in morphological criteria; chlorophyll a, b, and carotenoids; net photosynthetic rate; and the stomatal conductance and transpiration rate of watermelon seedlings, while intercellular carbon dioxide (CO₂) was significantly increased compared to the control plants (0.5 mg L⁻¹ B). Furthermore, excess boron accelerated the generation of reactive oxygen species (ROS), such as hydrogen peroxide (H₂O₂) and induced cellular oxidative injury. The application of exogenous SA significantly increased chlorophyll and carotenoid contents in plants exposed to excess B (10 mg L⁻¹), in line with the role of SA in alleviating chlorosis caused by B stress. Exogenously applied SA promoted photosynthesis and, consequently, biomass production in watermelon seedlings treated with a high level of B (10 mg L⁻¹) by reducing B accumulation, lipid peroxidation, and the generation of H₂O₂, while significantly increasing levels of the most reactive ROS, OH⁻. SA also activated antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) and protected the seedlings from an ROS induced cellular burst. In conclusion, SA can be used to alleviate the adverse effects of excess boron.

Acid soils are characterized by deficiencies in essential nutrient elements, oftentimes phosphorus (P), along with toxicities of metal elements, such as aluminum (Al), manganese (Mn), and cadmium (Cd), each of which significantly limits crop production. In recent years, impressive progress has been made in revealing mechanisms underlying tolerance to high concentrations of Al, Mn, and Cd. Phosphorus is an essential nutrient element that can alleviate exposure to potentially toxic levels of Al, Mn, and Cd. In this review, recent advances in elucidating the genes responsible for the uptake, translocation, and redistribution of Al, Mn, and Cd in plants are first summarized, as are descriptions of the mechanisms conferring resistance to these toxicities. Then, literature highlights information on interactions of P nutrition with Al, Mn, and Cd toxicities, particularly possible mechanisms driving P alleviation of these toxicities, along with potential applications for crop improvement on acid soils. The roles of plant phosphate (Pi) signaling and associated gene regulatory networks relevant for coping with Al, Mn, and Cd toxicities, are also discussed. To develop varieties adapted to acid soils, future work needs to further decipher involved signaling pathways and key regulatory elements, including roles fulfilled by intracellular Pi signaling. The development of new strategies for remediation of acid soils should integrate the mechanisms of these interactions between limiting factors in acid soils.

While the effects of carbon on soil nitrogen (N) cycle have been extensively studied, it is not clearly understood how co-existing macronutrients, such as phosphorus (P), affect the N cycle in agroecosystems. In this study, P amendment effects on nitrification in a fertile agricultural soil were investigated under a typical N-P amendment rate. In a laboratory incubation study, soils were amended with urea, monopotassium phosphate and a mixture of urea and monopotassium phosphate at the same rate. In soils that received no amendments (control), P only, urea only, and urea plus P amendment, nitrification occurred within the first five days, with an average net nitrification rate of 5.30, 5.77, 16.66 and 9.00 mg N kg⁻¹d⁻¹, respectively. Interestingly, nitrification in urea-treated soils was retarded by P addition where a N:P ratio seemed to be a key factor impeding nitrification. This was also supported by the response of ammonia-oxidizing bacteria (AOB), which was more sensitive to P addition than ammonia-oxidizing archaea (AOA). The outcome of this study showed that application of P fertilizer suppressed the nitrification process in urea amended soil, suggesting that a synergistic aspect of N and P nutrient management should be further explored to retard N losses from agricultural systems.