Background and objective: Leukemia is one of the fatal diseases and their morbidity and mortality rates increases day by day all over the world. This piece of research study was designed in order to find out the prevalence of different types of leukemia in Khyber Pakhtunkhwa, Pakistan during January 2015 to December 2016. Material and Method: The retrospective research study was carried out at Institute of Radiotherapy and Nuclear Medicine (IRNUM) Peshawar. A data of 400 admitted patients of leukemia were evaluated. Result: It was observed that acute leukemia (80%) was more prevalent than chronic leukemia (20%). Amongst types of leukemia, Acute Lymphocytic Leukemia (ALL) 49.5% (n=198) was more prevalent than Acute Myelogenous Leukemia (AML) 31.25% (n=125), Chronic Myelogenous Leukemia (CML) 10% (n=40) and Chronic Lymphocytic Leukemia (CLL) 9.25% (n=37) was less prevalent in this study. It was also found that leukemia was more prevalent in male patients 64.5% (n=258) as compared to females 35.5% (n=142) and male to female ratio was 1.8:1. Most of the patients were under the age of 20 years. Conclusion: Acute leukemia was more prevalent than chronic leukemia during this study in this part of the country and needs to be address. Bangladesh Journal of Medical Science Vol.18(2) 2019 p.222-227

Due to the low content, few studies are focused on the volatile compounds of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (PR, syn. Lamiophlomis rotata (Benth.) Kudô). However, such volatile compounds may have important pharmacological activities such as antioxidant, anticancer. In order to identify the chemical markers (CMs) in the essential oils (Es) and explore the potential bioactivities, we first conduct a thoroughly investigation on the volatile compounds and evaluate their anti-oxidant activities (AOAs), to the best of our knowledge. Light yellow Es with a fresh and elegant smell are obtained by hydro-distillation with an average yield of 0.11% (mL/g). After separation through cryoprecipitation, the crystals (Cs) and EOs removed crystals (RCs) were gotten, respectively. A total of 81 components are qualified and quantified in the Es, Cs and RCs, in which 44 ones are first reported from PR. As for content, the main compounds are long-chain fatty acids (LCFAs) and their esters. Among them, palmitic acid (PA), myristic acid (MA), linoleic acid (LA), oleic acid (OA) and methyl palmitate are highlighted, Besides, hexahydrofarnesyl acetone and phytol are also prominent Four kinds of C13-norisoprenoids are first reported from PR, in which, trans-β-damascenone is potent in flavour. In sum, these eight representative compounds can be chosen as the CMs. The AOAs of Es, Cs, RCs and four CMs such as PA, MA, LA and OA are evaluated by in vitro assays. In general, PA and MA present pro-oxidant activities (POAs) or weak AOAs. LA and OA show POAs or weak AOAs in lower concentration group, whereas demonstrate medium or strong AOAs in higher concentration group. Usually, the RCs demonstrate stronger AOAs and the Cs present weaker AOAs compared with those of the corresponding Es, which should be related to their different content of PA. This study can give some hints on the utilization of such Es which are abundant in LCFAs.

The antioxidant properties and characteristic metabolites of Phlomoides rotata (P. rotata) essential oils (EOs) remain largely unexplored. To address this gap, we combined metabolomic profiling with in vitro antioxidant assays to systematically characterize EOs from P. rotata. Cryoprecipitation of EOs yielded two fractions: crystals (Crs) and crystal-free EOs (CEs). GC-MS identified 125 components (84.41-94.86%), including 94 novel reports in P. rotata. Dominant constituents were long-chain fatty acids (LCFAs, 42.33-75.73%) and their esters (3.44-15.21%), notably palmitic acid (14.49-63.13%), myristic acid, linoleic acid, oleic acid, and methyl palmitate. Eleven norisoprenoids, including trans-β-damascenone, were discovered—a fivefold increase from previously reported diversity—with their biosynthetic pathways elucidated. Eleven chemical markers were established, encompassing geraniol, linalool, trans-β-damascenone, hexahydrofarnesyl acetone, palmitic acid, α-terpineol, myristic acid, phytol, linoleic acid, oleic acid, and methyl palmitate. Palmitic acid, myristic acid, methyl palmitate, and hexahydrofarnesyl acetone showed negligible or pro-oxidative activity. Linoleic acid, oleic acid, trans-β-damascenone, and phytol exhibited concentration-dependent antioxidant effects. Phytol demonstrated exceptional potency, surpassing quercetin in cellular antioxidant activity at 80 μmol/L. CEs exhibited significantly stronger antioxidant capacity in both DPPH and ABTS assays compared to EOs and Crs. Palmitic acid depletion in CEs was identified as a critical factor driving enhanced antioxidant performance. This study not only establishes P. rotata EOs as a rich source of phytol-based antioxidants but also deciphers the dual roles of LCFAs, providing a foundation for developing natural antioxidants.

Due to the low content, few studies are focused on the essential oils (EOs) of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (syn. Lamiophlomis rotata (Benth.) Kudô. This plant has been used to treat rheumatic arthritis and grasserie in China. However, such EOs may have important pharmacological activities such as anti-cancer. To identify the chemical markers (CM) in the EOs and evaluate their antioxidant activities (AAs), we firstly conduct a thoroughly investigation on their chemicals and AAs, to the best of our knowledge. Light yellow EOs with fresh and elegant smell are obtained by hydro-distillation with average yield 0.11% (volume mL/weight g). The crystals are separated from the EOs through cryoprecipitation, respectively. A total of 81 components are qualified and quantified in the EOs, crystals and EOs removed crystals, in which 44 ones are first reported. As for content, the main compounds are long-chain fatty acids (FAs) and their esters. The most abundant one n-hexadecanoic acid (palmitic acid, PA) accounting for 43.15-54.8%, 58.49-64.57% and 15.9-41.1%, in the EOs, crystals and EOs removed crystals, respectively. Seven compounds including PA, tetradecanoic acid, linoleic acid, oleic acid, methyl hexadecanoate, hexahydrofarnesyl acetone and phytol can be chosen a the CMs in these EOs. The AAs are evaluated through in vitro assays. PA presents pro-oxidant activities in a concentration dependence manner. Usually, the EOs removed crystals demonstrate stronger AAs, and the crystals demonstrate weaker AAs compared with that of the corresponding EOs, which is related to the different content of PA in these samples. This study can give some hints for the utilization of such EOs which are abundant in FAs such as PA.