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A continuous increase in global population is demanding more development and industrialization, which leads to the production of various waste such as municipal wastewater, agricultural waste, industrial waste, medical waste, electronic wastes, etc
Shashi Kant Bhatia. Wastewater Based Microbial Biorefinery for Bioenergy Production. Sustainability 2021, 13, 9214 .
AMA StyleShashi Kant Bhatia. Wastewater Based Microbial Biorefinery for Bioenergy Production. Sustainability. 2021; 13 (16):9214.
Chicago/Turabian StyleShashi Kant Bhatia. 2021. "Wastewater Based Microbial Biorefinery for Bioenergy Production." Sustainability 13, no. 16: 9214.
Whole-cell systems offer many benefits for biochemical production, such as relatively easy enzyme control and higher tolerance toward harsh environments, than purified enzymes. These systems can be applied to many bioconversion reactions, but they sometimes require cofactor regeneration units to support reactions at high substrate concentrations. Here, we examined l-glutamate oxidase (GOX) from Streptomyces sp. X119-6, which produces α-ketoglutarate (α-KG) from l-glutamate, and catalase (KatE) from Escherichia coli, which removes hydrogen peroxide generated by GOX. After optimizing the expression vector, pH, strains, culture conditions, and isopropyl β-d-1-thiogalactopyranoside concentration, we compared their efficiency to that of a previously reported GOX from Streptomyces mobaraensis. Our results indicated that GOX from Streptomyces sp. X119-6 and KatE increased α-KG production by 2.76-fold. This GOX required high levels of α-KG as an amino donor to convert 5-aminovaleric acid to glutaric acid. Performing the reaction at pH 8 enabled us to avoid the exogenous addition of catalase, but severe substrate inhibition was observed, resulting in the production of 287 mM glutaric acid. This α-KG regeneration system has potential for improving production in various aminotransferase systems.
Sion Ham; Yeong-Hoon Han; Sang Hyun Kim; Min Ju Suh; Jang Yeon Cho; Hong-Ju Lee; See-Hyoung Park; Kyungmoon Park; Jung-Oh Ahn; Jeong Chan Joo; Shashi Kant Bhatia; Yung-Hun Yang. Application of l-glutamate oxidase from Streptomyces sp. X119-6 with catalase (KatE) to whole-cell systems for glutaric acid production in Escherichia coli. Korean Journal of Chemical Engineering 2021, 1 -7.
AMA StyleSion Ham, Yeong-Hoon Han, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Hong-Ju Lee, See-Hyoung Park, Kyungmoon Park, Jung-Oh Ahn, Jeong Chan Joo, Shashi Kant Bhatia, Yung-Hun Yang. Application of l-glutamate oxidase from Streptomyces sp. X119-6 with catalase (KatE) to whole-cell systems for glutaric acid production in Escherichia coli. Korean Journal of Chemical Engineering. 2021; ():1-7.
Chicago/Turabian StyleSion Ham; Yeong-Hoon Han; Sang Hyun Kim; Min Ju Suh; Jang Yeon Cho; Hong-Ju Lee; See-Hyoung Park; Kyungmoon Park; Jung-Oh Ahn; Jeong Chan Joo; Shashi Kant Bhatia; Yung-Hun Yang. 2021. "Application of l-glutamate oxidase from Streptomyces sp. X119-6 with catalase (KatE) to whole-cell systems for glutaric acid production in Escherichia coli." Korean Journal of Chemical Engineering , no. : 1-7.
Carbon constraints, as well as the growing hazard of greenhouse gas emissions, have accelerated research into all possible renewable energy and fuel sources. Microbial electrolysis cells (MECs), a novel technology able to convert soluble organic matter into energy such as hydrogen gas, represent the most recent breakthrough. While research into energy recovery from wastewater using microbial electrolysis cells is fascinating and a carbon-neutral technology that is still mostly limited to lab-scale applications, much more work on improving the function of microbial electrolysis cells would be required to expand their use in many of these applications. The present limiting issues for effective scaling up of the manufacturing process include the high manufacturing costs of microbial electrolysis cells, their high internal resistance and methanogenesis, and membrane/cathode biofouling. This paper examines the evolution of microbial electrolysis cell technology in terms of hydrogen yield, operational aspects that impact total hydrogen output in optimization studies, and important information on the efficiency of the processes. Moreover, life-cycle assessment of MEC technology in comparison to other technologies has been discussed. According to the results, MEC is at technology readiness level (TRL) 5, which means that it is ready for industrial development, and, according to the techno-economics, it may be commercialized soon due to its carbon-neutral qualities.
Pooja Dange; Soumya Pandit; Dipak Jadhav; Poojhaa Shanmugam; Piyush Gupta; Sanjay Kumar; Manu Kumar; Yung-Hun Yang; Shashi Bhatia. Recent Developments in Microbial Electrolysis Cell-Based Biohydrogen Production Utilizing Wastewater as a Feedstock. Sustainability 2021, 13, 8796 .
AMA StylePooja Dange, Soumya Pandit, Dipak Jadhav, Poojhaa Shanmugam, Piyush Gupta, Sanjay Kumar, Manu Kumar, Yung-Hun Yang, Shashi Bhatia. Recent Developments in Microbial Electrolysis Cell-Based Biohydrogen Production Utilizing Wastewater as a Feedstock. Sustainability. 2021; 13 (16):8796.
Chicago/Turabian StylePooja Dange; Soumya Pandit; Dipak Jadhav; Poojhaa Shanmugam; Piyush Gupta; Sanjay Kumar; Manu Kumar; Yung-Hun Yang; Shashi Bhatia. 2021. "Recent Developments in Microbial Electrolysis Cell-Based Biohydrogen Production Utilizing Wastewater as a Feedstock." Sustainability 13, no. 16: 8796.
Crude oil is a major energy source that is exploited globally to achieve economic growth. To meet the growing demands for oil, in an environment of stringent environmental regulations and economic and technical pressure, industries have been required to develop novel oil salvaging techniques. The remaining ~70% of the world’s conventional oil (one-third of the available total petroleum) is trapped in depleted and marginal reservoirs, and could thus be potentially recovered and used. The only means of extracting this oil is via microbial enhanced oil recovery (MEOR). This tertiary oil recovery method employs indigenous microorganisms and their metabolic products to enhance oil mobilization. Although a significant amount of research has been undertaken on MEOR, the absence of convincing evidence has contributed to the petroleum industry’s low interest, as evidenced by the issuance of 400+ patents on MEOR that have not been accepted by this sector. The majority of the world’s MEOR field trials are briefly described in this review. However, the presented research fails to provide valid verification that the microbial system has the potential to address the identified constraints. Rather than promising certainty, MEOR will persist as an unverified concept unless further research and investigations are carried out.
Marzuqa Quraishi; Shashi Bhatia; Soumya Pandit; Piyush Gupta; Vivek Rangarajan; Dibyajit Lahiri; Sunita Varjani; Sanjeet Mehariya; Yung-Hun Yang. Exploiting Microbes in the Petroleum Field: Analyzing the Credibility of Microbial Enhanced Oil Recovery (MEOR). Energies 2021, 14, 4684 .
AMA StyleMarzuqa Quraishi, Shashi Bhatia, Soumya Pandit, Piyush Gupta, Vivek Rangarajan, Dibyajit Lahiri, Sunita Varjani, Sanjeet Mehariya, Yung-Hun Yang. Exploiting Microbes in the Petroleum Field: Analyzing the Credibility of Microbial Enhanced Oil Recovery (MEOR). Energies. 2021; 14 (15):4684.
Chicago/Turabian StyleMarzuqa Quraishi; Shashi Bhatia; Soumya Pandit; Piyush Gupta; Vivek Rangarajan; Dibyajit Lahiri; Sunita Varjani; Sanjeet Mehariya; Yung-Hun Yang. 2021. "Exploiting Microbes in the Petroleum Field: Analyzing the Credibility of Microbial Enhanced Oil Recovery (MEOR)." Energies 14, no. 15: 4684.
In this study, a newly isolated halotolerant strain Oceanisphaera arctica YHY1, capable of hydrolyzing seafood processing waste chitin biomass, is reported. Microbial fuel cells fed with 1% chitin and 40 g L−1 as the optimum salt concentration demonstrated stable electricity generation until 216 h (0.228 mA/cm2). N-acetyl-D-glucosamine (GlcNAc) was the main by-product in the chitin degradation, reaching a maximum concentration of 192.01 mg g−1 chitin at 120 h, whereas lactate, acetate, propionate, and butyrate were the major metabolites detected in the chitin degradation. O. arctica YHY1 utilized the produced GlcNAc, lactate, acetate, and propionate as the electron donors to generate the electric current. Cyclic voltammetry (CV) investigation revealed the participation of outer membrane-bound cytochromes, with extracellular redox mediators partly involved in the electron transfer mechanism. Furthermore, the changes in structural and functional groups in chitin after degradation were analyzed using FTIR and XRD. Therefore, the ability of O. arctica YHY1 to utilize waste chitin biomass under high salinities can be explored to treat seafood processing brine or high salt wastewater containing chitin with concurrent electricity generation.
Ranjit Gurav; Shashi Bhatia; Tae-Rim Choi; Hyun-Joong Kim; Hong-Ju Lee; Jang-Yeon Cho; Sion Ham; Min-Ju Suh; Sang-Hyun Kim; Sun-Ki Kim; Dong-Won Yoo; Yung-Hun Yang. Seafood Processing Chitin Waste for Electricity Generation in a Microbial Fuel Cell Using Halotolerant Catalyst Oceanisphaera arctica YHY1. Sustainability 2021, 13, 8508 .
AMA StyleRanjit Gurav, Shashi Bhatia, Tae-Rim Choi, Hyun-Joong Kim, Hong-Ju Lee, Jang-Yeon Cho, Sion Ham, Min-Ju Suh, Sang-Hyun Kim, Sun-Ki Kim, Dong-Won Yoo, Yung-Hun Yang. Seafood Processing Chitin Waste for Electricity Generation in a Microbial Fuel Cell Using Halotolerant Catalyst Oceanisphaera arctica YHY1. Sustainability. 2021; 13 (15):8508.
Chicago/Turabian StyleRanjit Gurav; Shashi Bhatia; Tae-Rim Choi; Hyun-Joong Kim; Hong-Ju Lee; Jang-Yeon Cho; Sion Ham; Min-Ju Suh; Sang-Hyun Kim; Sun-Ki Kim; Dong-Won Yoo; Yung-Hun Yang. 2021. "Seafood Processing Chitin Waste for Electricity Generation in a Microbial Fuel Cell Using Halotolerant Catalyst Oceanisphaera arctica YHY1." Sustainability 13, no. 15: 8508.
Microorganisms including actinomycetes, archaea, bacteria, fungi, yeast, and micro algae are the auspicious source of vital bioactive compounds. In this review, the existing state of the art re-garding antimicrobial molecules from microorganisms has been summarized. The potential an-timicrobial compounds from actinomycetes, particularly Streptomyces sp.; archaea; fungi including endophytic and marine-derived fungi, mushroom; yeast, and microalgae were briefly described. Furthermore, this review briefly summarized the activity and mode of action of bacteriocins, a ribosomally synthesized antimicrobial peptides product of Eurotium sp., Streptomyces parvulus, S. thermophiles, Lactococcus lactis, etc. Bacteriocins have inherent properties such as targeting multi-ple-drug resistant pathogens, which allows them to be considered next-generation antibiotics. Similarly, Glarea lozoyensis derived antifungal lipohexpeptides i.e., pneumocandins, inhibits 1,3-β-glucan synthase of the fungal cell wall and acts as a precursor for the synthesis of caspo-fungin, is also elaborated. In conclusion, this review highlights the possibility of using microor-ganisms as an antimicrobial resource for biotechnological, nutraceutical, and pharmaceutical ap-plications. However, more investigations are still required to separate, purify, and characterize these bioactive compounds and transfer these primary drugs into clinically approved antibiotics.
Alka Rani; Khem Chand Saini; Felix Bast; Sunita Varjani; Sanjeet Mehariya; Shashi Kant Bhatia; Neeta Sharma; Christiane Funk. A Review on Microorganisms-Derived Products as Potential Antimicrobial Agents. 2021, 1 .
AMA StyleAlka Rani, Khem Chand Saini, Felix Bast, Sunita Varjani, Sanjeet Mehariya, Shashi Kant Bhatia, Neeta Sharma, Christiane Funk. A Review on Microorganisms-Derived Products as Potential Antimicrobial Agents. . 2021; ():1.
Chicago/Turabian StyleAlka Rani; Khem Chand Saini; Felix Bast; Sunita Varjani; Sanjeet Mehariya; Shashi Kant Bhatia; Neeta Sharma; Christiane Funk. 2021. "A Review on Microorganisms-Derived Products as Potential Antimicrobial Agents." , no. : 1.
PIN-FORMED (PIN) genes play a crucial role in regulating polar auxin distribution in diverse developmental processes, including tropic responses, embryogenesis, tissue differentiation, and organogenesis. However, the role of PIN-mediated auxin transport in various plant species is poorly understood. Currently, no information is available about this gene family in wheat (Triticum aestivum L.). In the present investigation, we identified the PIN gene family in wheat to understand the evolution of PIN-mediated auxin transport and its role in various developmental processes and under different biotic and abiotic stress conditions. In this study, we performed genome-wide analysis of the PIN gene family in common wheat and identified 44 TaPIN genes through a homology search, further characterizing them to understand their structure, function, and distribution across various tissues. Phylogenetic analyses led to the classification of TaPIN genes into seven different groups, providing evidence of an evolutionary relationship with Arabidopsis thaliana and Oryza sativa. A gene exon/intron structure analysis showed a distinct evolutionary path and predicted the possible gene duplication events. Further, the physical and biochemical properties, conserved motifs, chromosomal, subcellular localization, transmembrane domains, and three-dimensional (3D) structure were also examined using various computational approaches. Cis-elements analysis of TaPIN genes showed that TaPIN promoters consist of phytohormone, plant growth and development, and stress-related cis-elements. In addition, expression profile analysis also revealed that the expression patterns of the TaPIN genes were different in different tissues and developmental stages. Several members of the TaPIN family were induced during biotic and abiotic stress. Moreover, the expression patterns of TaPIN genes were verified by qRT-PCR. The qRT-PCR results also show a similar expression with slight variation. Therefore, the outcome of this study provides basic genomic information on the expression of the TaPIN gene family and will pave the way for dissecting the precise role of TaPINs in plant developmental processes and different stress conditions.
Manu Kumar; Bhagwat Kherawat; Prajjal Dey; Debanjana Saha; Anupama Singh; Shashi Bhatia; Gajanan Ghodake; Avinash Kadam; Hyun-Uk Kim; Manorama; Sang-Min Chung; Mahipal Kesawat. Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L. International Journal of Molecular Sciences 2021, 22, 7396 .
AMA StyleManu Kumar, Bhagwat Kherawat, Prajjal Dey, Debanjana Saha, Anupama Singh, Shashi Bhatia, Gajanan Ghodake, Avinash Kadam, Hyun-Uk Kim, Manorama, Sang-Min Chung, Mahipal Kesawat. Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L. International Journal of Molecular Sciences. 2021; 22 (14):7396.
Chicago/Turabian StyleManu Kumar; Bhagwat Kherawat; Prajjal Dey; Debanjana Saha; Anupama Singh; Shashi Bhatia; Gajanan Ghodake; Avinash Kadam; Hyun-Uk Kim; Manorama; Sang-Min Chung; Mahipal Kesawat. 2021. "Genome-Wide Identification and Characterization of PIN-FORMED (PIN) Gene Family Reveals Role in Developmental and Various Stress Conditions in Triticum aestivum L." International Journal of Molecular Sciences 22, no. 14: 7396.
With the advent of global industrialisation and adaptation of smart life there is rise in anthropogenic pollution especially in water. Remediation of the pollutants (such as metals, and dyes) present in industrial effluents is possible via microbes and algae present in the environment. Microbes are used in a microbial fuel cell (MFC) for remediation of various organic and inorganic pollutants. However, for industrial scale application coupling the MFCs with photocatalytic and photoelectric fuel cell has a potential in improving the output of power. It can also be used for remediation of pollutants more expeditiously, conserving fossil fuels, cleaning environment, hence making the coupled hybrid fuel cell to run economically. Furthermore, such MFC inbuilt with algae in living or powder form give additional value addition products like biofuel, polysaccharides, biopolymers, and polyhydroxy alkanoates etc. This review provides bird’s eye view on the removal of environmental pollutants by different biological sources like bacteria and algae. The article is focussed on diatoms as potential algae since they are rich source of crude oil and high value added products in a hybrid photocatalytic MFC. It also covers bottle necks, challenges and future in this field of research.
Vandana Vinayak; Mohd Jahir Khan; Sunita Varjani; Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Shashi Kant Bhatia. Microbial fuel cells for remediation of environmental pollutants and value addition: Special focus on coupling diatom microbial fuel cells with photocatalytic and photoelectric fuel cells. Journal of Biotechnology 2021, 338, 5 -19.
AMA StyleVandana Vinayak, Mohd Jahir Khan, Sunita Varjani, Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Shashi Kant Bhatia. Microbial fuel cells for remediation of environmental pollutants and value addition: Special focus on coupling diatom microbial fuel cells with photocatalytic and photoelectric fuel cells. Journal of Biotechnology. 2021; 338 ():5-19.
Chicago/Turabian StyleVandana Vinayak; Mohd Jahir Khan; Sunita Varjani; Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Shashi Kant Bhatia. 2021. "Microbial fuel cells for remediation of environmental pollutants and value addition: Special focus on coupling diatom microbial fuel cells with photocatalytic and photoelectric fuel cells." Journal of Biotechnology 338, no. : 5-19.
Wastewater management and its treatment have revolutionized the industry sector into many innovative techniques. However, the cost of recycling via chemical treatment has major issues especially in economically poor sectors. On the offset, one of the most viable and economical techniques to clean wastewater is by growing microalgae in it. Since wastewater is rich in nitrates, phosphates and other trace elements, the environment is suitable for the growth of microalgae. On the other side, the cost of harvesting microalgae for its secondary metabolites is burgeoning. While simultaneously growing of microalgae in photobioreactors requires regular feeding of the nutrients and maintenance which increases the cost of operation and hence cost of its end products. The growth of microalgae in waste waters makes the process not only economical but they also manufacture more amounts of value added products. However, harvesting of these values added products is still a cumbersome task. On the offset, it has been observed that pretreating the microalgal biomass with ultrasonication allows easy oozing of the secondary metabolites like oil, proteins, carbohydrates and methane at much lower cost than that required for their extraction. Among microalgae diatoms are more robust and have immense crude oil and are rich in various value added products. However, due to their thick silica walls they do not ooze the metabolites until the mechanical force on their walls reaches certain threshold energy. In this review recycling of wastewater using microalgae and its pretreatment via ultrasonication with special reference to diatoms is critically discussed. Perspectives on circular bioeconomy and knowledge gaps for employing microalgae to recycle wastewater have been comprehensively narrated.
Mohd Jahir Khan; Harish Mangesh; Ankesh Ahirwar; Benoit Schoefs; Arivalagan Pugazhendhi; Sunita Varjani; Karthik Rajendran; Shashi Kant Bhatia; Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Vandana Vinayak. Insights into diatom microalgal farming for treatment of wastewater and pretreatment of algal cells by ultrasonication for value creation. Environmental Research 2021, 201, 111550 .
AMA StyleMohd Jahir Khan, Harish Mangesh, Ankesh Ahirwar, Benoit Schoefs, Arivalagan Pugazhendhi, Sunita Varjani, Karthik Rajendran, Shashi Kant Bhatia, Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Vandana Vinayak. Insights into diatom microalgal farming for treatment of wastewater and pretreatment of algal cells by ultrasonication for value creation. Environmental Research. 2021; 201 ():111550.
Chicago/Turabian StyleMohd Jahir Khan; Harish Mangesh; Ankesh Ahirwar; Benoit Schoefs; Arivalagan Pugazhendhi; Sunita Varjani; Karthik Rajendran; Shashi Kant Bhatia; Ganesh Dattatraya Saratale; Rijuta Ganesh Saratale; Vandana Vinayak. 2021. "Insights into diatom microalgal farming for treatment of wastewater and pretreatment of algal cells by ultrasonication for value creation." Environmental Research 201, no. : 111550.
Many pathogenic viral pandemics have caused threats to global health; the COVID-19 pandemic is the latest. Its transmission is growing exponentially all around the globe, putting constraints on the health system worldwide. A novel coronavirus, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), causes this pandemic. Many candidate vaccines are available at this time for COVID-19, and there is a massive international race underway to procure as many vaccines as possible for each country. However, due to heavy global demand, there are strains in global vaccine production. The use of a plant biotechnology-based expression system for vaccine production also represents one part of this international effort, which is to develop plant-based heterologous expression systems, virus-like particles (VLPs)-vaccines, antiviral drugs, and a rapid supply of antigen-antibodies for detecting kits and plant origin bioactive compounds that boost the immunity and provide tolerance to fight against the virus infection. This review will look at the plant biotechnology platform that can provide the best fight against this global pandemic.
Manu Kumar; Nisha Kumari; Nishant Thakur; Shashi Bhatia; Ganesh Saratale; Gajanan Ghodake; Bhupendra Mistry; Hemasundar Alavilli; D. Kishor; Xueshi Du; Sang-Min Chung. A Comprehensive Overview on the Production of Vaccines in Plant-Based Expression Systems and the Scope of Plant Biotechnology to Combat against SARS-CoV-2 Virus Pandemics. Plants 2021, 10, 1213 .
AMA StyleManu Kumar, Nisha Kumari, Nishant Thakur, Shashi Bhatia, Ganesh Saratale, Gajanan Ghodake, Bhupendra Mistry, Hemasundar Alavilli, D. Kishor, Xueshi Du, Sang-Min Chung. A Comprehensive Overview on the Production of Vaccines in Plant-Based Expression Systems and the Scope of Plant Biotechnology to Combat against SARS-CoV-2 Virus Pandemics. Plants. 2021; 10 (6):1213.
Chicago/Turabian StyleManu Kumar; Nisha Kumari; Nishant Thakur; Shashi Bhatia; Ganesh Saratale; Gajanan Ghodake; Bhupendra Mistry; Hemasundar Alavilli; D. Kishor; Xueshi Du; Sang-Min Chung. 2021. "A Comprehensive Overview on the Production of Vaccines in Plant-Based Expression Systems and the Scope of Plant Biotechnology to Combat against SARS-CoV-2 Virus Pandemics." Plants 10, no. 6: 1213.
Polyhydroxyalkanoates (PHAs) are bioplastic substitutes for petroleum-derived plastics that may help to reduce the increasing environmental impact of plastic pollution. Among them, polyhydroxybutyrate (PHB) is a promising biopolymer, incentivizing many researchers to search for PHB-producing and PHB-degrading bacteria for improved PHB utilization. Many novel PHB-producing microorganisms have been discovered; however, relatively few PHB-degrading bacteria have been identified. Six PHB-degrading bacteria were found in marine soil and investigated their PHB-degrading abilities under various temperature and salinity conditions using solid-media based culture. Finally, thermotolerant and halotolerant PHB-degrader Bacillus sp. JY14 was selected. PHB degradation was confirmed by monitoring changes in the physical and chemical properties of PHB films incubated with Bacillus sp. JY14 using scanning electron microscopy, Fourier-transform infrared spectroscopy, and gel permeation chromatography. Further, PHB degradation ability of Bacillus sp. JY14 was measured in liquid culture by gas chromatography. After 14 days of cultivation with PHB film, Bacillus sp. JY14 achieved approximately 98% PHB degradation. Applying various bioplastics to assess the bacteria's biodegradation capabilities, the result showed that Bacillus sp. JY14 could also degrade P(3HB-co-4HB) and P(3HB-co-3HV). Overall, this study identified a thermotolerant and halotolerant bacteria capable of PHB degradation under solid and liquid conditions. These results suggest that this bacteria could be utilized to degrade various PHAs.
Jang Yeon Cho; Sol Lee Park; Hong-Ju Lee; Sang Hyun Kim; Min Ju Suh; Sion Ham; Shashi Kant Bhatia; Ranjit Gurav; See-Hyoung Park; Kyungmoon Park; Dongwon Yoo; Yung-Hun Yang. Polyhydroxyalkanoates (PHAs) degradation by the newly isolated marine Bacillus sp. JY14. Chemosphere 2021, 283, 131172 .
AMA StyleJang Yeon Cho, Sol Lee Park, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Sion Ham, Shashi Kant Bhatia, Ranjit Gurav, See-Hyoung Park, Kyungmoon Park, Dongwon Yoo, Yung-Hun Yang. Polyhydroxyalkanoates (PHAs) degradation by the newly isolated marine Bacillus sp. JY14. Chemosphere. 2021; 283 ():131172.
Chicago/Turabian StyleJang Yeon Cho; Sol Lee Park; Hong-Ju Lee; Sang Hyun Kim; Min Ju Suh; Sion Ham; Shashi Kant Bhatia; Ranjit Gurav; See-Hyoung Park; Kyungmoon Park; Dongwon Yoo; Yung-Hun Yang. 2021. "Polyhydroxyalkanoates (PHAs) degradation by the newly isolated marine Bacillus sp. JY14." Chemosphere 283, no. : 131172.
Biodiesel is an environmentally friendly, biodegradable, and renewable source of energy. It can be used as an alternate source of energy to reduce overburden on fossil-based fuels. The use of waste cooking oil (WCO) for biodiesel production may solve its management problem and provide additional revenue. Rhodococcus is an oleaginous microbe that can utilize various feedstock as a carbon source for growth and lipid production. In this study, WCO was used as a carbon source and the effect of various surfactants on growth and lipids accumulation was studied. Production media components optimized using response surface design and cultivation in optimized media i.e., WCO (1.15%): NH4Cl (0.09%): NaCl (0.97%): PEG (0.05%) resulted in 3.42 ± 0.06 g CDW/L biomass production, 2.39 ± 0.04 g/L lipid production with 70 ± 4.0% w/w lipid accumulation. Produced lipids were analyzed for fatty acid methyl esters (FAMEs) composition and composed of palmitoleic acid (16:1, 61.68%) > palmitic acid (C16:0, 21.48%) > myristic acid (C14:0, 12.95%) > stearic acid (C18:0, 2.35%) > pentadecanoic acid (C15:0, 0.74%) > heptadecanoic acid (C17:0, 0.72%) > oleic acid (C18:1, 0.05%). Properties of biodiesel depend on FAMEs composition and analyzed as cetane number (CN, 56.5), iodine value (IV, 63.7), cold filter plugging point (CFPP, −9.6), cloud point (CP, 6.3 °C), pour point (PP, 0.028 °C), oxidation stability (OS, infinity), high heating value (HHV, 38.9), kinematic viscosity (υ, 3.2 mm2/s), and density (ρ, 0.87 g/cm3). The properties of produced biodiesel satisfy the international standards EN14214 and IS15607 and suggest that biodiesel produced from WCO oil can be used as an energy source.
Shashi Kant Bhatia; Ranjit Gurav; Yong-Keun Choi; Hong-Ju Lee; Sang Hyun Kim; Min Ju Suh; Jang Yeon Cho; Sion Ham; Sang Ho Lee; Kwon-Young Choi; Yung-Hun Yang. Rhodococcus sp. YHY01 a microbial cell factory for the valorization of waste cooking oil into lipids a feedstock for biodiesel production. Fuel 2021, 301, 121070 .
AMA StyleShashi Kant Bhatia, Ranjit Gurav, Yong-Keun Choi, Hong-Ju Lee, Sang Hyun Kim, Min Ju Suh, Jang Yeon Cho, Sion Ham, Sang Ho Lee, Kwon-Young Choi, Yung-Hun Yang. Rhodococcus sp. YHY01 a microbial cell factory for the valorization of waste cooking oil into lipids a feedstock for biodiesel production. Fuel. 2021; 301 ():121070.
Chicago/Turabian StyleShashi Kant Bhatia; Ranjit Gurav; Yong-Keun Choi; Hong-Ju Lee; Sang Hyun Kim; Min Ju Suh; Jang Yeon Cho; Sion Ham; Sang Ho Lee; Kwon-Young Choi; Yung-Hun Yang. 2021. "Rhodococcus sp. YHY01 a microbial cell factory for the valorization of waste cooking oil into lipids a feedstock for biodiesel production." Fuel 301, no. : 121070.
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (phaC). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate-co-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-co-mcl PHA determined a glass transition temperature (Tg) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 104, weight average molecular weight of 9.1 × 104, and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low Tg, and low crystallinity, indicating its potential use in medical applications.
Tae-Rim Choi; Ye-Lim Park; Hun-Suk Song; Sun Lee; Sol Park; Hye Lee; Hyun-Joong Kim; Shashi Bhatia; Ranjit Gurav; Kwon-Young Choi; Yoo Lee; Yung-Hun Yang. Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6. Polymers 2021, 13, 1398 .
AMA StyleTae-Rim Choi, Ye-Lim Park, Hun-Suk Song, Sun Lee, Sol Park, Hye Lee, Hyun-Joong Kim, Shashi Bhatia, Ranjit Gurav, Kwon-Young Choi, Yoo Lee, Yung-Hun Yang. Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6. Polymers. 2021; 13 (9):1398.
Chicago/Turabian StyleTae-Rim Choi; Ye-Lim Park; Hun-Suk Song; Sun Lee; Sol Park; Hye Lee; Hyun-Joong Kim; Shashi Bhatia; Ranjit Gurav; Kwon-Young Choi; Yoo Lee; Yung-Hun Yang. 2021. "Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6." Polymers 13, no. 9: 1398.
Polyhydroxyalkanoates (PHAs) are attractive new bioplastics for the replacement of plastics derived from fossil fuels. With their biodegradable properties, they have also recently been applied to the medical field. As poly(3-hydroxybutyrate) produced by wild-type Ralstonia eutropha has limitations with regard to its physical properties, it is advantageous to synthesize co- or terpolymers with medium-chain-length monomers. In this study, tung oil, which has antioxidant activity due to its 80% α-eleostearic acid content, was used as a carbon source and terpolymer P(53 mol% 3-hydroxybytyrate-co-2 mol% 3-hydroxyvalerate-co-45 mol% 3-hydroxyhexanoate) with a high proportion of 3-hydroxyhexanoate was produced in R. eutropha Re2133/pCB81. To avail the benefits of α-eleostearic acid in the tung oil-based medium, we performed partial harvesting of PHA by using a mild water wash to recover PHA and residual tung oil on the PHA film. This resulted in a film coated with residual tung oil, showing antioxidant activity. Here, we report the first application of tung oil as a substrate for PHA production, introducing a high proportion of hydroxyhexanoate monomer into the terpolymer. Additionally, the residual tung oil was used as an antioxidant coating, resulting in the production of bioactive PHA, expanding the applicability to the medical field.
Hye Lee; Sun Lee; Sol Park; Tae-Rim Choi; Hun-Suk Song; Hyun-Joong Kim; Shashi Bhatia; Ranjit Gurav; Yun-Gon Kim; June-Hyung Kim; Kwon-Young Choi; Yung-Hun Yang. Tung Oil-Based Production of High 3-Hydroxyhexanoate-Containing Terpolymer Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate-co-3-Hydroxyhexanoate) Using Engineered Ralstonia eutropha. Polymers 2021, 13, 1084 .
AMA StyleHye Lee, Sun Lee, Sol Park, Tae-Rim Choi, Hun-Suk Song, Hyun-Joong Kim, Shashi Bhatia, Ranjit Gurav, Yun-Gon Kim, June-Hyung Kim, Kwon-Young Choi, Yung-Hun Yang. Tung Oil-Based Production of High 3-Hydroxyhexanoate-Containing Terpolymer Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate-co-3-Hydroxyhexanoate) Using Engineered Ralstonia eutropha. Polymers. 2021; 13 (7):1084.
Chicago/Turabian StyleHye Lee; Sun Lee; Sol Park; Tae-Rim Choi; Hun-Suk Song; Hyun-Joong Kim; Shashi Bhatia; Ranjit Gurav; Yun-Gon Kim; June-Hyung Kim; Kwon-Young Choi; Yung-Hun Yang. 2021. "Tung Oil-Based Production of High 3-Hydroxyhexanoate-Containing Terpolymer Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate-co-3-Hydroxyhexanoate) Using Engineered Ralstonia eutropha." Polymers 13, no. 7: 1084.
Weeds are undesired plants and dominant competitors of desired agriculture, horticulture, or other ornamental plants. Weed plants have high vigor, persistence, produce more seeds, have high seed dormancy, and have the ability to spread quickly.
Vijay Kumar; Virender Kumar; Ranjit G. Gurav; Yung-Hun Yang; Shashi Kant Bhatia. Utilization of Weed Plants for Biochemicals and Bioactive Compounds Production. Applications of Paleoenvironmental Techniques in Estuarine Studies 2021, 183 -206.
AMA StyleVijay Kumar, Virender Kumar, Ranjit G. Gurav, Yung-Hun Yang, Shashi Kant Bhatia. Utilization of Weed Plants for Biochemicals and Bioactive Compounds Production. Applications of Paleoenvironmental Techniques in Estuarine Studies. 2021; ():183-206.
Chicago/Turabian StyleVijay Kumar; Virender Kumar; Ranjit G. Gurav; Yung-Hun Yang; Shashi Kant Bhatia. 2021. "Utilization of Weed Plants for Biochemicals and Bioactive Compounds Production." Applications of Paleoenvironmental Techniques in Estuarine Studies , no. : 183-206.
Bacillus subtilis is regarded as a suitable host for biochemical production owing to its excellent growth and bioresource utilization characteristics. In addition, the distinct endogenous metabolic pathways and the suitability of the heterologous pathways have made B. subtilis a robust and promising host for producing biochemicals, such as: bioalcohols; bioorganic acids (lactic acids, α-ketoglutaric acid, and γ-aminobutyric acid); biopolymers (poly(γ-glutamic acid, polyhydroxyalkanoates (PHA), and polysaccharides and monosaccharides (N-acetylglucosamine, xylooligosaccharides, and hyaluronic acid)); and bioflocculants. Also for producing oligopeptides and functional peptides, owing to its efficient protein secretion system. Several metabolic and genetic engineering techniques, such as target gene overexpression and inactivation of bypass pathways, have led to the improvement in production titers and product selectivity. In this review article, recent progress in the utilization of robust B. subtilis-based host systems for biomass conversion and biochemical production has been highlighted, and the prospects of such host systems are suggested.
Seo A. Park; Shashi Kant Bhatia; Hyun A. Park; Seo Yeong Kim; Pamidimarri D. V. N. Sudheer; Yung-Hun Yang; Kwon-Young Choi. Bacillus subtilis as a robust host for biochemical production utilizing biomass. Critical Reviews in Biotechnology 2021, 1 -34.
AMA StyleSeo A. Park, Shashi Kant Bhatia, Hyun A. Park, Seo Yeong Kim, Pamidimarri D. V. N. Sudheer, Yung-Hun Yang, Kwon-Young Choi. Bacillus subtilis as a robust host for biochemical production utilizing biomass. Critical Reviews in Biotechnology. 2021; ():1-34.
Chicago/Turabian StyleSeo A. Park; Shashi Kant Bhatia; Hyun A. Park; Seo Yeong Kim; Pamidimarri D. V. N. Sudheer; Yung-Hun Yang; Kwon-Young Choi. 2021. "Bacillus subtilis as a robust host for biochemical production utilizing biomass." Critical Reviews in Biotechnology , no. : 1-34.
Oxidative stress originates from an elevated intracellular level of free oxygen radicals that cause lipid peroxidation, protein denaturation, DNA hydroxylation, and apoptosis, ultimately impairing cell viability. Antioxidants scavenge free radicals and reduce oxidative stress, which further helps to prevent cellular damage. Medicinal plants, fruits, and spices are the primary sources of antioxidants from time immemorial. In contrast to plants, microorganisms can be used as a source of antioxidants with the advantage of fast growth under controlled conditions. Further, microbe-based antioxidants are nontoxic, noncarcinogenic, and biodegradable as compared to synthetic antioxidants. The present review aims to summarize the current state of the research on the antioxidant activity of microorganisms including actinomycetes, bacteria, fungi, protozoa, microalgae, and yeast, which produce a variety of antioxidant compounds, i.e., carotenoids, polyphenols, vitamins, and sterol, etc. Special emphasis is given to the mechanisms and signaling pathways followed by antioxidants to scavenge Reactive Oxygen Species (ROS), especially for those antioxidant compounds that have been scarcely investigated so far.
Alka Rani; Khem Saini; Felix Bast; Sanjeet Mehariya; Shashi Bhatia; Roberto Lavecchia; Antonio Zuorro. Microorganisms: A Potential Source of Bioactive Molecules for Antioxidant Applications. Molecules 2021, 26, 1142 .
AMA StyleAlka Rani, Khem Saini, Felix Bast, Sanjeet Mehariya, Shashi Bhatia, Roberto Lavecchia, Antonio Zuorro. Microorganisms: A Potential Source of Bioactive Molecules for Antioxidant Applications. Molecules. 2021; 26 (4):1142.
Chicago/Turabian StyleAlka Rani; Khem Saini; Felix Bast; Sanjeet Mehariya; Shashi Bhatia; Roberto Lavecchia; Antonio Zuorro. 2021. "Microorganisms: A Potential Source of Bioactive Molecules for Antioxidant Applications." Molecules 26, no. 4: 1142.
Coronavirus Disease 19 (COVID-19), due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has become an on-going global health emergency affecting over 94 million cases with more than 2 million deaths globally. Primarily identified as atypical pneumonia, it has developed into severe acute respiratory distress syndrome (ARDS), a multi-organ dysfunction with associated fatality. Ever since its emergence, COVID-19 with its plethora of clinical presentations has signalled its dynamic nature and versatility of the disease process. Being a disease with droplet transmission has now assumed the proportion of a suspected airborne nature which, once proved, poses a Herculean task to control. Because of the wide distribution of the human angiotensin-converting enzyme-2 (hACE2) receptors, known for its transmission, we envisage its multiorgan spread and extensive disease distribution. Thus, an extensive review of the extrapulmonary organotropism of SARS-CoV-2 with organ-specific pathophysiology and associated manifestations like dermatological complications, myocardial dysfunction, gastrointestinal symptoms, neurologic illnesses, hepatic and renal injury is needed urgently. The plausible mechanism of site-specific viral invasion is also discussed to give a comprehensive understanding of disease complexity, to help us to focus on research priorities and therapeutic strategies to counter the disease progression. A note on the latest advancements in vaccine research will enlighten the scientific world and equip it for better preparedness.
Vikram Thakur; Radha Ratho; Pradeep Kumar; Shashi Bhatia; Ishani Bora; Gursimran Mohi; Shailendra Saxena; Manju Devi; Dhananjay Yadav; Sanjeet Mehariya. Multi-Organ Involvement in COVID-19: Beyond Pulmonary Manifestations. Journal of Clinical Medicine 2021, 10, 446 .
AMA StyleVikram Thakur, Radha Ratho, Pradeep Kumar, Shashi Bhatia, Ishani Bora, Gursimran Mohi, Shailendra Saxena, Manju Devi, Dhananjay Yadav, Sanjeet Mehariya. Multi-Organ Involvement in COVID-19: Beyond Pulmonary Manifestations. Journal of Clinical Medicine. 2021; 10 (3):446.
Chicago/Turabian StyleVikram Thakur; Radha Ratho; Pradeep Kumar; Shashi Bhatia; Ishani Bora; Gursimran Mohi; Shailendra Saxena; Manju Devi; Dhananjay Yadav; Sanjeet Mehariya. 2021. "Multi-Organ Involvement in COVID-19: Beyond Pulmonary Manifestations." Journal of Clinical Medicine 10, no. 3: 446.
Biowaste management is a challenging job as it is high in nutrient content and its disposal in open may cause a serious environmental and health risk. Traditional technologies such as landfill, bio-composting, and incineration are used for biowaste management. To gain revenue from biowaste researchers around the world focusing on the integration of biowaste management with other commercial products such as volatile fatty acids (VFA), biohydrogen, and bioplastic (polyhydroxyalkanoates (PHA)), etc. PHA production from various biowastes such as lignocellulosic biomass, municipal waste, waste cooking oils, biodiesel industry waste, and syngas has been reported successfully. Various nutrient factors i.e., carbon and nitrogen source concentration and availability of dissolved oxygen are crucial factors for PHA production. This review is an attempt to summarize the recent advancements in PHA production from various biowaste, its downstream processing, and other challenges that need to overcome making bioplastic an alternate for synthetic plastic.
Shashi Kant Bhatia; Sachin V. Otari; Jong-Min Jeon; Ranjit Gurav; Yong-Keun Choi; Ravi Kant Bhatia; Arivalagan Pugazhendhi; Vinod Kumar; J. Rajesh Banu; Jeong-Jun Yoon; Kwon-Young Choi; Yung-Hun Yang. Biowaste-to-bioplastic (polyhydroxyalkanoates): Conversion technologies, strategies, challenges, and perspective. Bioresource Technology 2021, 326, 124733 .
AMA StyleShashi Kant Bhatia, Sachin V. Otari, Jong-Min Jeon, Ranjit Gurav, Yong-Keun Choi, Ravi Kant Bhatia, Arivalagan Pugazhendhi, Vinod Kumar, J. Rajesh Banu, Jeong-Jun Yoon, Kwon-Young Choi, Yung-Hun Yang. Biowaste-to-bioplastic (polyhydroxyalkanoates): Conversion technologies, strategies, challenges, and perspective. Bioresource Technology. 2021; 326 ():124733.
Chicago/Turabian StyleShashi Kant Bhatia; Sachin V. Otari; Jong-Min Jeon; Ranjit Gurav; Yong-Keun Choi; Ravi Kant Bhatia; Arivalagan Pugazhendhi; Vinod Kumar; J. Rajesh Banu; Jeong-Jun Yoon; Kwon-Young Choi; Yung-Hun Yang. 2021. "Biowaste-to-bioplastic (polyhydroxyalkanoates): Conversion technologies, strategies, challenges, and perspective." Bioresource Technology 326, no. : 124733.
Biohydrogen is a clean and renewable source of energy. It can be produced by using technologies such as thermochemical, electrolysis, photoelectrochemical and biological, etc. Among these technologies, the biological method (dark fermentation) is considered more sustainable and ecofriendly. Dark fermentation involves anaerobic microbes which degrade carbohydrate rich substrate and produce hydrogen. Lignocellulosic biomass is an abundantly available raw material and can be utilized as an economic and renewable substrate for biohydrogen production. Although there are many hurdles, continuous advancements in lignocellulosic biomass pretreatment technology, microbial fermentation (mixed substrate and co-culture fermentation), the involvement of molecular biology techniques, and understanding of various factors (pH, T, addition of nanomaterials) effect on biohydrogen productivity and yield render this technology efficient and capable to meet future energy demands. Further integration of biohydrogen production technology with other products such as bio-alcohol, volatile fatty acids (VFAs), and methane have the potential to improve the efficiency and economics of the overall process. In this article, various methods used for lignocellulosic biomass pretreatment, technologies in trends to produce and improve biohydrogen production, a coproduction of other energy resources, and techno-economic analysis of biohydrogen production from lignocellulosic biomass are reviewed.
Shashi Kant Bhatia; Sujit Sadashiv Jagtap; Ashwini Ashok Bedekar; Ravi Kant Bhatia; Karthik Rajendran; Arivalagan Pugazhendhi; Christopher V. Rao; A.E. Atabani; Gopalakrishnan Kumar; Yung-Hun Yang. Renewable biohydrogen production from lignocellulosic biomass using fermentation and integration of systems with other energy generation technologies. Science of The Total Environment 2020, 765, 144429 .
AMA StyleShashi Kant Bhatia, Sujit Sadashiv Jagtap, Ashwini Ashok Bedekar, Ravi Kant Bhatia, Karthik Rajendran, Arivalagan Pugazhendhi, Christopher V. Rao, A.E. Atabani, Gopalakrishnan Kumar, Yung-Hun Yang. Renewable biohydrogen production from lignocellulosic biomass using fermentation and integration of systems with other energy generation technologies. Science of The Total Environment. 2020; 765 ():144429.
Chicago/Turabian StyleShashi Kant Bhatia; Sujit Sadashiv Jagtap; Ashwini Ashok Bedekar; Ravi Kant Bhatia; Karthik Rajendran; Arivalagan Pugazhendhi; Christopher V. Rao; A.E. Atabani; Gopalakrishnan Kumar; Yung-Hun Yang. 2020. "Renewable biohydrogen production from lignocellulosic biomass using fermentation and integration of systems with other energy generation technologies." Science of The Total Environment 765, no. : 144429.