Chitin, and especially its deacetylated variant chitosan, has many applications, e.g. as carrier material for pharmaceutical drugs or as a flocculant in wastewater treatment. Despite its versatility and accessibility, chitin, the second most abundant polysaccharide on Earth, has so far been commercially extracted only from crustaceans and to a minor extent from fungi. Insects are a viable alternative source of chitin, but they have not been exploited in the past due to limited availability. Today however, for the sustainable production of animal feed, insect farming is being developed substantially. The availability of large quantities of insect biomass and chitin‐rich side products such as exuviae and exoskeletons has been increasing. This review provides an overview of recently published studies of chitin extraction from insects, its subsequent conversion into chitosan and the primary analytical methods used to characterize insect‐based chitin and chitosan. We have discovered a large number of research articles published over the past 20 years, confirming the increased attention being received by chitin and chitosan production from insects. Despite numerous publications, we identified several knowledge gaps, such as a lack of data concerning chitin purification degree and chitosan yield. Furthermore, analytical methods used to obtain physicochemical characteristics, structural information and chemical composition meet basic qualitative requirements but do not satisfy the need for a more quantitative evaluation. Despite the current shortcomings that need to be overcome, this review presents encouraging data on the use of insects as an alternative source of chitin and chitosan in the future. © 2020 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

The exponential increase of global demand for proteins and lipids can no longer be satisfied by classical sources. High amounts of CO₂ produced by intensive livestock breeding and its effects on the environment are the main factors that prevent the use of animals as primary sources for proteins and lipids, calling for the use of new sustainable sources, such as insects. The massive breeding of bioconverter insects as a feed source has been a major topic in recent years, with both economic and scientific aspects related to rearing and subsequent processing optimization. The larvae of Hermetia illucens (Diptera: Stratiomyidae) (also known as Black Soldier Fly) can be used for the eco-sustainable production of proteins and lipids with high biological and economic value. Lipids can be obtained from BSF bioconversion processes and are present in high quantities in the last instar larvae and prepupae. Fats obtained from BSF are used as animal feed ingredients, in the formulation of several products for personal care, and in biodiesel production. To enable the use of insect-derived lipids, it is important to understand how to optimize their extraction. Here, we summarize the published information on the composition, the extraction methods, and the possible applications of the BSF lipid component.

Chitin and its derivatives are attracting great interest in cosmetic and cosmeceutical fields, thanks to their antioxidant and antimicrobial properties, as well as their biocompatibility and biodegradability. The classical source of chitin, crustacean waste, is no longer sustainable and fungi, a possible alternative, have not been exploited at an industrial scale yet. On the contrary, the breeding of bioconverting insects, especially of the Diptera Hermetia illucens, is becoming increasingly popular worldwide. Therefore, their exoskeletons, consisting of chitin as a major component, represent a waste stream of facilities that could be exploited for many applications. Insect chitin, indeed, suggests its application in the same fields as the crustacean biopolymer, because of its comparable commercial characteristics. This review reports several cosmetic and cosmeceutical applications based on chitin and its derivatives. In this context, chitin nanofibers and nanofibrils, produced from crustacean waste, have proved to be excellent cosmeceutical active compounds and carriers of active ingredients in personal care. Consequently, the insect-based chitin, its derivatives and their complexes with hyaluronic acid and lignin, as well as with other chitin-derived compounds, may be considered a new appropriate potential polymer to be used in cosmetic and cosmeceutical fields.

Purpose: Chitin purification from remains (pupal exuviae after metamorphosis to adult flies) of Hermetia illucens farming was optimized performing demineralization, deproteinization and bleaching under different conditions. The optimal parameters to obtain high-purity chitin were determined. Methods: Dried and ground pupal exuviae, whose composition was initially determined, were demineralized using six different acids. Proteins were removed with a NaOH treatment in which temperature, molarity and duration were varied in a randomized experiment. Bleaching was carried out testing ten different chemicals, including NaOCl, H₂O₂, solvent mixtures and enzymes. The efficiency of each step was determined to assess the optimal conditions for each of them. The resulting chitin was subjected to spectroscopic characterization. Results: The highest demineralization efficiency (90%) was achieved using 0.5 M formic acid for 2 h at 40 °C, confirming the validity of organic acids as a more sustainable alternative to inorganic acids. The treatment with 1.25 M NaOH at 90 °C for 4 h showed the highest deproteinization efficiency, removing 96% of the proteins. Temperature and NaOH concentration were the significant parameters for deproteinization efficiency. The most efficient bleaching treatment was with 6% NaOCl at 60 °C for 1 h (67% efficiency). H₂O₂ could also be a valid alternative to avoid environmental risk related to chlorine-containing compounds. At the end of the purification process 17% of the original biomass was retained with a chitin content of 85%, corresponding to a chitin yield of 14% related to the initial biomass. Solid-state nuclear magnetic resonance showed that the purified chitin had a degree of acetylation of 96% and X-ray powder diffraction gave a crystallinity index of 74%. Conclusion: This investigation shows an optimized method for extraction of high-purity chitin from H. illucens pupal exuviae, supporting the validity of insect-farming remains as source of this versatile biopolymer. Graphical

The effects of in-hive tasks on the honey bee (Apis mellifera L.) ventriculus microbial profile were investigated by analysing honey bees belonging to six in-hive tasks (newly-emerged, nurses, wax builder, guardians, young foragers, old foragers). Total viable aerobic bacterial count, Enterobacteriaceae, staphylococci, Escherichia coli, lactic acid bacteria, Pseudomonas spp., aerobic bacterial endospores, and Enterococcus spp. were determined using a culture-based method. Significant in-hive tasks-dependent microbial profiles were detected for all cultured bacteria (p < 0.001), with the lowest values for newly-emerged and wax builder and the highest for old foragers. Pseudomonas spp. and Staphylococcus spp. were quantitatively lower in young foragers than in old foragers, while they were absent in the other investigated in-hive tasks. E. coli and Enterococcus spp. were detected only in old foragers. 1Lactic acid bacteria were higher in nurses and old foragers compared to the other in-hive tasks, while the newly-emerged showed the lowest amount of lactic acid bacteria. The results from this investigation showed that ventriculus microbiological profile of Apis mellifera ligustica undergoes changes based on the in-hive task, and, as concerns foragers, on their age.

The recent decades witnessed the collapse of honey bee colonies at a global level. The major drivers of this collapse include both individual and synergic pathogen actions, threatening the colonies’ survival. The need to define the epidemiological pattern of the pathogens that are involved has led to the establishment of monitoring programs in many countries, Italy included. In this framework, the health status of managed honey bees in the Emilia–Romagna region (northern Italy) was assessed, throughout the year 2021, on workers from 31 apiaries to investigate the presence of major known and emerging honey bee pathogens. The prevalence and abundance of DWV, KBV, ABPV, CBPV, Nosema ceranae, and trypanosomatids (Lotmaria passim, Crithidia mellificae, Crithidia bombi) were assessed by molecular methods. The most prevalent pathogen was DWV, followed by CBPV and N. ceranae. Trypanosomatids were not found in any of the samples. Pathogens had different peaks in abundance over the months, showing seasonal trends that were related to the dynamics of both bee colonies and Varroa destructor infestation. For some of the pathogens, a weak but significant correlation was observed between abundance and geographical longitude. The information obtained in this study increases our understanding of the epidemiological situation of bee colonies in Emilia–Romagna and helps us to implement better disease prevention and improved territorial management of honey bee health.