In this work, we develop a multi-mode microscopic hyperspectral imager (MMHI) for the detection of biological samples in transmission imaging, reflection imaging and fluorescence mode. A hyperspectral image cube can be obtained with 5 μm spatial resolution and 3 nm spectral resolution through push-broom line scanning. To avoid possible shadows produced by the high magnification objective with a short working distance, two illumination patterns are designed to ensure the co-axiality of the illumination and detection. Three experiments for the detection of zebrafish and fingerprints and the classification of disaster-causing microalgae verify the good capability and functionality of the system. Based on the detected spectra, we can observe the impacts of β-carotene and melanin in zebrafish, hemoglobin in the fingertip, and chlorophyll in microalgae, respectively. Multi-modes can be switched freely according to the application requirement and characteristics of different samples, like transmission mode for the transparent/translucent sample, reflection mode for the opaque sample and fluorescence mode for the fluorescent sample. The MMHI system also has strong potential for the non-invasive and high-speed sensing of bio or clinical samples.

In this paper, we experimentally demonstrate a 450-nm laser underwater optical wireless transmission system, in which a comprehensive investigation is made to show the significant performance improvement of the discrete Fourier transform spread discrete multi-tone (DFT-S DMT) as compared to the conventional quadrature amplitude modulation discrete multi-tone (QAM-DMT) modulation. DFT-S DMT outperforms QAM-DMT in terms of bit error rate (BER) performance due to low peak-to-average-power ratio (PAPR) and capability of counteracting high frequency fading in a band-limited underwater optical wireless communication (UOWC) system. In order to avoid signal-to-noise-ratio (SNR) degradation at fringe subcarriers, several zeros are padded at the edge of each block before DFT-S operation. The experimental results show the superiority of DFT-S DMT compared with QAM-DMT in different water environments, including turbidities, bubbles, and water flow. Data rates of ~16.16 Gbps and ~13.96 Gbps at a BER of 3.8×10 $^\text{-3}$ are achieved by 16-QAM DFT-S DMT and 16-QAM-DMT over a 5-m water channel, respectively, which indicates that capacity enhancement of ~2.2 Gbps is obtained by the DFT-S DMT. Meanwhile, over 3-dB receiver sensitivity improvement can always be achieved by the DFT-S DMT at the tested underwater transmission distances. Combined with adaptive bit-power loading, 20.04 Gbps over a 5-m "clear ocean" channel transmission with a single laser diode (LD) is demonstrated. For a 35-m water link, the distance-data rate product reaches 498.4 Gbps*m. To the best of our knowledge, both the data rate and distance-data rate product are the largest among all the results reported for a single visible LD. Aimed at high-speed deep ocean applications, the studies are promising for future UOWC research.

A novel light-sheet microscopy (LSM) system that uses the laser triangulation method to quantitatively calculate and analyze the surface topography of opaque samples is discussed. A spatial resolution of at least 10 μm in z-direction, 10 μm in x-direction and 25 μm in y-direction with a large field-of-view (FOV) is achieved. A set of sample measurements that verify the system′s functionality in various applications are presented. The system has a simple mechanical structure, such that the spatial resolution is easily improved by replacement of the objective, and a linear calibration formula, which enables convenient system calibration. As implemented, the system has strong potential for, e.g., industrial sample line inspections, however, since the method utilizes reflected/scattered light, it also has the potential for three-dimensional analysis of translucent and layered structures.