Heat stress is one of the major threats to wheat production in many wheat-growing areas of the world as it causes severe yield loss at the reproductive stage. In the current study, 28 crosses were developed using 11 parental lines, including 7 female lines and 4 male testers following line × tester matting design in 2018–2019. Twenty-eight crosses along with their 11 parental lines were sown in a randomized complete block design in triplicate under optimal and heat stress conditions. Fifteen different morpho-physiological and grain quality parameters were recorded at different growth stages. Analysis of variance illustrated the presence of highly significant differences among wheat genotypes for all traits under both optimal and heat stress conditions. The results of combining ability unveiled the predominant role of non-additive gene action in the inheritance of almost all the studied traits under both conditions. Among parents, 3 parental lines WL-27, WT-39, and WL-57 showed good combining ability under both normal and heat stress conditions. Among crosses, WL-8 × WT-17, WL-37 × WT-17, WL-7 × WT-39, and WL-37 × WT-39 portrayed the highest specific combining ability effects for grain yield and its related traits under optimal as well as heat stress conditions. Biplot and cluster analysis confirmed the results of general and specific combining ability by showing that these wheat crosses belonged to a highly productive and heat tolerant cluster. Correlation analysis revealed a significantly positive correlation of grain yield with net photosynthetic rate, thousand-grain rate, and the number of grains per spike. The designated parental lines and their crosses were selected for future breeding programs in the development of heat resilient, climate-smart wheat genotypes.

Wheat rusts, including leaf, stripe, and stem rust, have been a threat to global food security due to their devastating impact on wheat yields. In recent years, significant strides have been made in understanding wheat rusts, focusing on disease spread mechanisms, the discovery of new host resistance genes, and the molecular basis of rust pathogenesis. This review summarizes the latest approaches and studies in wheat rust research that provide a comprehensive understanding of disease mechanisms and new insights into control strategies. Recent advances in genetic resistance using modern genomics techniques, as well as molecular mechanisms of rust pathogenesis and host resistance, are discussed. In addition, innovative management strategies, including the use of fungicides and biological control agents, are reviewed, highlighting their role in combating wheat rust. This review also emphasizes the impact of climate change on rust epidemiology and underscores the importance of developing resistant wheat varieties along with adaptive management practices. Finally, gaps in knowledge are identified and suggestions for future research are made. This review aims to inform researchers, agronomists, and policy makers, and to contribute to the development of more effective and sustainable wheat rust control strategies.

Leaf senescence (LS), the final phase in leaf development, is an important and precisely regulated process crucial for plant well-being and the redistribution of nutrients. It is intricately controlled by various regulatory factors, including WRKY transcription factors (TFs). WRKYs are one of the most significant plant TF families, and several of them are differentially regulated and important during LS. Recent research has enhanced our understanding of the structural and functional characteristics of WRKY TFs, providing insights into their regulatory roles. This review aims to elucidate the genetic and molecular mechanisms underlying the intricate regulatory networks associated with LS by investigating the role of WRKY TFs. We seek to highlight the importance of WRKY-mediated signaling pathways in understanding LS, plant evolution, and response to varying environmental conditions. WRKY TFs exhibit specific DNA-binding activity at the N-terminus and dynamic interactions of the intrinsically disordered domain at the C-terminus with various proteins. These WRKY TFs not only control the activity of other WRKYs, but also interact with either WRKYs or other TFs, thereby fine- tuning the expression of target genes. By unraveling the complex interactions and regulatory mechanisms of WRKY TFs, this review broadens our knowledge of the genetic and molecular basis of LS. Understanding WRKY-mediated signalling pathways provides crucial insights into specific aspects of plant development, such as stress-induced senescence, and offers potential strategies for improving crop resilience to environmental stresses like drought and pathogen attacks. By targeting these pathways, it may be possible to enhance specific productivity traits, such as increased yield stability under adverse conditions, thereby contributing to more reliable agricultural outputs.