The aim of this study was to investigate the effects of different packaging systems on the shelf life of refrigerated ground beef. The ground beef samples were packaged as follows: AA (100% ambient air), 90O₂:10CO₂ (90% O₂ and 10% CO₂), 80O₂:20CO₂ (80% O₂ and 20% CO₂), 70O₂:30CO₂ (70% O₂ and 30% CO₂), 60O₂:40CO₂ (60% O₂ and 40% CO₂), 50O₂:50CO₂ (50% O₂ and 50% CO₂), 100O₂ (100% O₂), and VP (vacuum packaging). All treatments were analyzed daily for O₂ and CO₂ levels, pH, filtration time, total volatile basic nitrogen (TVB-N), aerobic mesophilic heterotrophic bacteria (AMHB), and aerobic psychrotrophic heterotrophic bacteria (APHB) over 20 days at 2 °C. All MAP systems had a decrease of O₂ and an increase of CO₂ levels during storage period (p < 0.05). Overall, the MAP systems were similarly able to decrease the pH and retard the increase of TVB-N and filtration time over the storage period (p > 0.05). Moreover, the MAP systems increased the lag phase and/or the generation time of both AMHB and APHB, extending the shelf life by 3 (90O₂:10CO₂), 4 (70O₂:30CO₂ and 100O₂), and 5 days (80O₂:20CO₂, 60O₂:40CO₂, 50O₂:50CO_2, and VP). All MAP systems were equally effective in retarding physicochemical degradation; however, 80O₂:20CO₂, 60O₂:40CO₂, 50O₂:50CO_2, and VP were the most effective in impairing bacterial growth and extending the shelf life of ground beef stored under refrigeration.

The aim of the present study was to evaluate the sensory acceptability limit of refrigerated fish through a multivariate approach, involving classic physicochemical and bacteriological indicators and considering different consumer profiles. The results of the survival analysis demonstrated that, in general, consumers still considered the fish to be suitable for purchase (4.128 days of storage), despite being microbiologically unsuitable for consumption. However, the subsequent division of consumers into clusters indicated that women and individuals with high income and education levels tend to reject fish with few days of storage (3.650 days), mainly due to discoloration, despite still being microbiologically suitable for consumption. Thus, these segments present a safer behavior regarding the purchase of fresh fish. The influence of different frequencies of fish consumption and age of consumers on the assessment of fish freshness was not clarified. The responsibility for ensuring safe and healthy products at the point of sale must lie with the producers and distributors. However, improving consumers’ ability to make good choices when buying fresh fish would bring social and economic benefits related to public health and to the seafood industry, because it would enable them to make relevant claims and demand their rights.

Researchers recognize the silent, negative and deleterious effects caused by mercury pollution in gold mining areas. Freshwater turtles are culturally part of the diet of riverside populations in the Amazon region and this area presents mercury (Hg) pollution issues mainly due to gold mining activities. Thus, this research aimed to evaluate the total mercury (THg) content in the different organs of Amazonian giant river turtle (Podocnemis expansa) and carry out a human health risk assessment associated with the consumption of these animals. This study was conducted in the Vila Balbina, municipality of Presidente Figueiredo, state of Amazonas, Brazil. Skin (n = 28), muscle (n = 19) and brain (n = 2) samples were analyzed by Atomic Absorption Spectrometry (TDA-AAS) and a DMA-80™ mercury analyzer was used for the total mercury determinations. The average values found for THg in the skin, muscle and brain samples were, respectively, 0.1045 mg·kg⁻¹, 0.1092 mg·kg⁻¹ and 0.0601 mg·kg⁻¹. Thus, THg was observed even though the P. expansa were kept in captivity, possibly due to previous contamination by air, water and food. The Hazard Quotient (HQ) was calculated considering a 9.07 g·day⁻¹ intake dose of P. expansa and the consumption of turtles once a week showed an HQ = 2.45, which may cause long-term injuries to human health. Although the muscle concentrations were below the maximum limit established by the World Health Organization (WHO) and Brazilian regulatory agencies, it is important to evaluate consumption factors such as amount ingested, frequency and animal gender, which may cause a potential risk to regular consumers due to mercury bioaccumulation. The WHO may consider various aspects in order to warn the Amazon population about the severity and silent hazard of this metal, especially due to the importance of this matrix in the region. This region urgently needs government actions to inhibit clandestine mining and to prevent future serious, chronic health problems of the entire population.

Birds of prey at higher trophic levels are particularly vulnerable to mercury pollution and can serve as indicators of trace elements in different ecosystems. Thus, the aim of this study was to evaluate the presence of mercury in feathers and livers in some birds of prey that occur in the State of Rio de Janeiro, Brazil. Eleven livers were collected from birds of pray during routine necropsies performed at the Rio de Janeiro Zoological Garden, and the feathers from animals in rehabilitation/release process. The results showed mercury concentrations ranged from 0.5 mg / kg to 1.3074 mg / kg in livers and from 0.3376 mg / kg to 3.000 mg / kg in feathers. Liver samples can be collected through invasive methods like biopsies, which involve anesthesia and handling of the bird, or during routine necropsies in zoological collections, breeding grounds, and from deceased birds found in the field, especially those affected by trampling or firearm injuries. Alternatively, analyzing feathers for mercury provides a practical, cost-effective, and non-invasive method that avoids risking harm to the bird's physical well-being.