Zernike phase contrast microscopy is extended and combined with a phase-shifting mechanism to perform quantitative phase measurements of microscopic objects. Dozens of discrete point light sources on a ring are constructed for illumination. For each point light source, three different levels of point-like phase steps are designed, which are alternatively located along a ring on a silica plate to perform phase retardation on the undiffracted (dc) component of the object waves. These three levels of the phase steps are respectively selected by rotating the silica plate. Thus, quantitative evaluation of phase specimens can be performed via phase-shifting mechanism. The proposed method has low “halo” and “shade-off” effects, low coherent noise level, and high lateral resolution due to the improved illumination scheme.

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The Talbot effect is analyzed when steel tape gratings are used. These gratings are made on a steel substrate, and, because of the manufacture process, both levels of the grating are rough with different roughness parameters. A theoretical analysis based on Fresnel regime, which considers the statistical properties of roughness, is developed. Analytical formulas that show a decreasing exponential dependence on the intensity in terms of the distance between the grating and the observation plane are obtained, and an experimental verification is also performed.

We analyze the self-imaging process produced by a transmission grating whose strips present two different roughness levels. This kind of grating periodically modulates the transmitted light owing only to the different microtopographic properties of the strips. In spite of the fact that the grating is not purely periodic, it produces a kind of self-image at Talbot distances. These self-images gradually appear as light propagates, but they are not present just after the grating, as occurs in amplitude or phase gratings. There exists a distance from the grating, which depends on the stochastic properties of roughness, from which the contrast of the self-images becomes stable. Important cases are analyzed in detail, such as low- and high-roughness limits. We assume for the calculations that the grating can be used in a mobile system. Simulations using the Rayleigh–Sommerfeld regime have been performed, which confirm the validity of the theoretical approach proposed in this work

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