This page has only limited features, please log in for full access.
Glioblastoma (GB), is the most common and aggressive malignant primary brain tumour in adults. Intra- and inter-tumour heterogeneity, infiltrative GB cell invasion and presence of therapy-resistant GB stem cells (GSCs) represent major obstacles to favourable prognosis and poor therapy response. Identifying the biomarkers of the most aggressive tumour cells and their more efficient targeting strategies are; therefore, crucial. Recently, transcription factor TRIM28 has been identified as a GB biomarker and, in this study, we have shown high expression of TRIM28 in GB and in low grade gliomas as well as higher expression in GSCs vs. differentiated GB cells, although in both cases not significant. We demonstrated significant in vitro inhibition of GB cells and GSCs invasiveness and spread in zebrafish brains in vivo by anti-TRIM28 selective nanobody NB237. TRIM28 was also enriched in GB (tumour) core and associated with the expression of stem cell genes, but was not prognostic for overall survival. However, based on the above results, we conclude that TRIM28 nanobody NB237 offers a new opportunity as a GB therapeutic tool.
Andrej Porčnik; Metka Novak; Barbara Breznik; Bernarda Majc; Barbara Hrastar; Neja Šamec; Alja Zottel; Ivana Jovčevska; Miloš Vittori; Ana Rotter; Radovan Komel; Tamara Lah Turnšek. TRIM28 Selective Nanobody Reduces Glioblastoma Stem Cell Invasion. Molecules 2021, 26, 5141 .
AMA StyleAndrej Porčnik, Metka Novak, Barbara Breznik, Bernarda Majc, Barbara Hrastar, Neja Šamec, Alja Zottel, Ivana Jovčevska, Miloš Vittori, Ana Rotter, Radovan Komel, Tamara Lah Turnšek. TRIM28 Selective Nanobody Reduces Glioblastoma Stem Cell Invasion. Molecules. 2021; 26 (17):5141.
Chicago/Turabian StyleAndrej Porčnik; Metka Novak; Barbara Breznik; Bernarda Majc; Barbara Hrastar; Neja Šamec; Alja Zottel; Ivana Jovčevska; Miloš Vittori; Ana Rotter; Radovan Komel; Tamara Lah Turnšek. 2021. "TRIM28 Selective Nanobody Reduces Glioblastoma Stem Cell Invasion." Molecules 26, no. 17: 5141.
In this study, we conducted and documented workshops and interviews in Norway and Slovenia to identify stakeholder and future generation opinions and mitigation strategies for solving one of the most prominent environmental issues: plastic pollution. As part of the EU H2020 project GoJelly, stakeholders were brought together to explore their perceptions on considering jellyfish mucus as a new resource to contribute to reducing plastic pollution from entering the marine environment. The study was conducted in the spring of 2019, in a context directly after the European Union (EU) announced its Directive to ban the most commonly used single-use plastic (SUP) items. The study applied the snowball method as a methodological choice to identify relevant stakeholders. Systems thinking was utilized as a participatory modelling approach, which allowed for the creation of conceptual mind maps from the various workshops and interviews, to understand consumers’ consciousness, and to map out ideas on plastic pollution reduction. Plastic pollution takes place on a global scale and stakeholders discussed their individual perceptions of national and international solutions that could be put in place to solve it, including the opportunities around utilizing jellyfish mucus to filter and capture micro- and nanoplastic. We found that industry stakeholders in both case areas were generally more accepting of policy and increased innovation moving forward, but placed weight on the scientific community to conduct more research on the pollution issue and propose solutions. Future generation stakeholders (youth aged 14–18), however, put emphasis on consumer behavior and buying patterns of single-use products fueling the plastic crisis.
Emily Cowan; Andy M. Booth; Andreas Misund; Katja Klun; Ana Rotter; Rachel Tiller. Single-Use Plastic Bans: Exploring Stakeholder Perspectives on Best Practices for Reducing Plastic Pollution. Environments 2021, 8, 81 .
AMA StyleEmily Cowan, Andy M. Booth, Andreas Misund, Katja Klun, Ana Rotter, Rachel Tiller. Single-Use Plastic Bans: Exploring Stakeholder Perspectives on Best Practices for Reducing Plastic Pollution. Environments. 2021; 8 (8):81.
Chicago/Turabian StyleEmily Cowan; Andy M. Booth; Andreas Misund; Katja Klun; Ana Rotter; Rachel Tiller. 2021. "Single-Use Plastic Bans: Exploring Stakeholder Perspectives on Best Practices for Reducing Plastic Pollution." Environments 8, no. 8: 81.
The worldwide microplastics pollution is a serious environmental and health problem that is currently not effectively mitigated. In this work we tested jellyfish mucus as a new bioflocculent material capable of sequestration of polystyrene microplastics in aqueous environments. Mucus material was collected from different jellyfish species and was used to trap fluorescently tagged polystyrene microspheres. The efficiency of removal was tested using varying concentrations of microplastics and mucus. The interaction between the microplastics and mucus was determined by viscosity measurements and confocal laser scanning microscopy. Different mucus preparation methods were also tested: freshly prepared, mechanically sheared, freeze-thawed, freeze-dried, and hydrolyzed mucus. The results demonstrate that jellyfish mucus can efficiently sequester polystyrene microplastics particles from the suspension. The fraction of the removed microplastics was highest with freshly prepared mucus and decreased with freeze-thawing and freeze-drying. The mucus ability to sequester microplastics was completely lost in the hydrolyzed mucus. The results imply that the intact jellyfish mucus has the potential to be used as a biopolymer capable of removing microplastics material.
Živa Lengar; Katja Klun; Iztok Dogsa; Ana Rotter; David Stopar. Sequestration of Polystyrene Microplastics by Jellyfish Mucus. Frontiers in Marine Science 2021, 8, 1 .
AMA StyleŽiva Lengar, Katja Klun, Iztok Dogsa, Ana Rotter, David Stopar. Sequestration of Polystyrene Microplastics by Jellyfish Mucus. Frontiers in Marine Science. 2021; 8 ():1.
Chicago/Turabian StyleŽiva Lengar; Katja Klun; Iztok Dogsa; Ana Rotter; David Stopar. 2021. "Sequestration of Polystyrene Microplastics by Jellyfish Mucus." Frontiers in Marine Science 8, no. : 1.
The increasing and rapid development in technologies, infrastructures, computational power, data availability and information flow has enabled rapid scientific advances. These entail transdisciplinary collaborations that maximize sharing of data and knowledge and, consequently, results, and possible technology transfer. However, in emerging scientific fields it is sometimes difficult to provide all necessary expertise within existing collaborative circles. This is especially true for marine biotechnology that directly addresses global societal challenges. This article describes the creation of a platform dedicated to facilitating the formation of short or mid-term collaborative networks in marine biotechnology. This online platform (https://www.ocean4biotech.eu/map/) enables experts (researchers and members of the marine biotechnology community in general) to have the possibility to showcase their expertise with the aim of being integrated into new collaborations/consortia on the one hand, or to use it as a search tool to complement the expertise in planned/running collaborations, on the other. The platform was created within the Ocean4Biotech (European transdisciplinary networking platform for marine biotechnology) Action, funded under the framework of the European Cooperation in Science and Technology (COST). To build the platform, an inquiry was developed to identify experts in marine biotechnology and its adjunct fields, to define their expertise, to highlight their infrastructures and facilities and to pinpoint the main bottlenecks in this field. The inquiry was open to all experts in the broad field of marine biotechnology, including non-members of the consortium. The inquiry (https://ee.kobotoolbox.org/single/UKVsBNtD) remains open for insertion of additional expertise and the resulting interactive map can be used as a display and search tool for establishing new collaborations.
Ana Rotter; Susana P. Gaudêncio; Katja Klun; Jan-Niklas Macher; Olivier P. Thomas; Irem Deniz; Christine Edwards; Ernesta Grigalionyte-Bembič; Zrinka Ljubešić; Johan Robbens; Giovanna Cristina Varese; Marlen I. Vasquez. A New Tool for Faster Construction of Marine Biotechnology Collaborative Networks. Frontiers in Marine Science 2021, 8, 1 .
AMA StyleAna Rotter, Susana P. Gaudêncio, Katja Klun, Jan-Niklas Macher, Olivier P. Thomas, Irem Deniz, Christine Edwards, Ernesta Grigalionyte-Bembič, Zrinka Ljubešić, Johan Robbens, Giovanna Cristina Varese, Marlen I. Vasquez. A New Tool for Faster Construction of Marine Biotechnology Collaborative Networks. Frontiers in Marine Science. 2021; 8 ():1.
Chicago/Turabian StyleAna Rotter; Susana P. Gaudêncio; Katja Klun; Jan-Niklas Macher; Olivier P. Thomas; Irem Deniz; Christine Edwards; Ernesta Grigalionyte-Bembič; Zrinka Ljubešić; Johan Robbens; Giovanna Cristina Varese; Marlen I. Vasquez. 2021. "A New Tool for Faster Construction of Marine Biotechnology Collaborative Networks." Frontiers in Marine Science 8, no. : 1.
Coastal countries have traditionally relied on the existing marine resources (e.g., fishing, food, transport, recreation, and tourism) as well as tried to support new economic endeavors (ocean energy, desalination for water supply, and seabed mining). Modern societies and lifestyle resulted in an increased demand for dietary diversity, better health and well-being, new biomedicines, natural cosmeceuticals, environmental conservation, and sustainable energy sources. These societal needs stimulated the interest of researchers on the diverse and underexplored marine environments as promising and sustainable sources of biomolecules and biomass, and they are addressed by the emerging field of marine (blue) biotechnology. Blue biotechnology provides opportunities for a wide range of initiatives of commercial interest for the pharmaceutical, biomedical, cosmetic, nutraceutical, food, feed, agricultural, and related industries. This article synthesizes the essence, opportunities, responsibilities, and challenges encountered in marine biotechnology and outlines the attainment and valorization of directly derived or bio-inspired products from marine organisms. First, the concept of bioeconomy is introduced. Then, the diversity of marine bioresources including an overview of the most prominent marine organisms and their potential for biotechnological uses are described. This is followed by introducing methodologies for exploration of these resources and the main use case scenarios in energy, food and feed, agronomy, bioremediation and climate change, cosmeceuticals, bio-inspired materials, healthcare, and well-being sectors. The key aspects in the fields of legislation and funding are provided, with the emphasis on the importance of communication and stakeholder engagement at all levels of biotechnology development. Finally, vital overarching concepts, such as the quadruple helix and Responsible Research and Innovation principle are highlighted as important to follow within the marine biotechnology field. The authors of this review are collaborating under the European Commission-funded Cooperation in Science and Technology (COST) Action Ocean4Biotech – European transdisciplinary networking platform for marine biotechnology and focus the study on the European state of affairs.
Ana Rotter; Michéle Barbier; Francesco Bertoni; Atle M. Bones; M. Leonor Cancela; Jens Carlsson; Maria F. Carvalho; Marta Cegłowska; Jerónimo Chirivella-Martorell; Meltem Conk Dalay; Mercedes Cueto; Thanos Dailianis; Irem Deniz; Ana R. Díaz-Marrero; Dragana Drakulovic; Arita Dubnika; Christine Edwards; Hjörleifur Einarsson; Ayşegül Erdoǧan; Orhan Tufan Eroldoǧan; David Ezra; Stefano Fazi; Richard J. FitzGerald; Laura M. Gargan; Susana P. Gaudêncio; Marija Gligora Udovič; Nadica Ivošević DeNardis; Rósa Jónsdóttir; Marija Kataržytė; Katja Klun; Jonne Kotta; Leila Ktari; Zrinka Ljubešić; Lada Lukić Bilela; Manolis Mandalakis; Alexia Massa-Gallucci; Inga Matijošytė; Hanna Mazur-Marzec; Mohamed Mehiri; Søren Laurentius Nielsen; Lucie Novoveská; Donata Overlingė; Giuseppe Perale; Praveen Ramasamy; Céline Rebours; Thorsten Reinsch; Fernando Reyes; Baruch Rinkevich; Johan Robbens; Eric Röttinger; Vita Rudovica; Jerica Sabotič; Ivo Safarik; Siret Talve; Deniz Tasdemir; Xenia Theodotou Schneider; Olivier P. Thomas; Anna Toruńska-Sitarz; Giovanna Cristina Varese; Marlen I. Vasquez. The Essentials of Marine Biotechnology. Frontiers in Marine Science 2021, 8, 1 .
AMA StyleAna Rotter, Michéle Barbier, Francesco Bertoni, Atle M. Bones, M. Leonor Cancela, Jens Carlsson, Maria F. Carvalho, Marta Cegłowska, Jerónimo Chirivella-Martorell, Meltem Conk Dalay, Mercedes Cueto, Thanos Dailianis, Irem Deniz, Ana R. Díaz-Marrero, Dragana Drakulovic, Arita Dubnika, Christine Edwards, Hjörleifur Einarsson, Ayşegül Erdoǧan, Orhan Tufan Eroldoǧan, David Ezra, Stefano Fazi, Richard J. FitzGerald, Laura M. Gargan, Susana P. Gaudêncio, Marija Gligora Udovič, Nadica Ivošević DeNardis, Rósa Jónsdóttir, Marija Kataržytė, Katja Klun, Jonne Kotta, Leila Ktari, Zrinka Ljubešić, Lada Lukić Bilela, Manolis Mandalakis, Alexia Massa-Gallucci, Inga Matijošytė, Hanna Mazur-Marzec, Mohamed Mehiri, Søren Laurentius Nielsen, Lucie Novoveská, Donata Overlingė, Giuseppe Perale, Praveen Ramasamy, Céline Rebours, Thorsten Reinsch, Fernando Reyes, Baruch Rinkevich, Johan Robbens, Eric Röttinger, Vita Rudovica, Jerica Sabotič, Ivo Safarik, Siret Talve, Deniz Tasdemir, Xenia Theodotou Schneider, Olivier P. Thomas, Anna Toruńska-Sitarz, Giovanna Cristina Varese, Marlen I. Vasquez. The Essentials of Marine Biotechnology. Frontiers in Marine Science. 2021; 8 ():1.
Chicago/Turabian StyleAna Rotter; Michéle Barbier; Francesco Bertoni; Atle M. Bones; M. Leonor Cancela; Jens Carlsson; Maria F. Carvalho; Marta Cegłowska; Jerónimo Chirivella-Martorell; Meltem Conk Dalay; Mercedes Cueto; Thanos Dailianis; Irem Deniz; Ana R. Díaz-Marrero; Dragana Drakulovic; Arita Dubnika; Christine Edwards; Hjörleifur Einarsson; Ayşegül Erdoǧan; Orhan Tufan Eroldoǧan; David Ezra; Stefano Fazi; Richard J. FitzGerald; Laura M. Gargan; Susana P. Gaudêncio; Marija Gligora Udovič; Nadica Ivošević DeNardis; Rósa Jónsdóttir; Marija Kataržytė; Katja Klun; Jonne Kotta; Leila Ktari; Zrinka Ljubešić; Lada Lukić Bilela; Manolis Mandalakis; Alexia Massa-Gallucci; Inga Matijošytė; Hanna Mazur-Marzec; Mohamed Mehiri; Søren Laurentius Nielsen; Lucie Novoveská; Donata Overlingė; Giuseppe Perale; Praveen Ramasamy; Céline Rebours; Thorsten Reinsch; Fernando Reyes; Baruch Rinkevich; Johan Robbens; Eric Röttinger; Vita Rudovica; Jerica Sabotič; Ivo Safarik; Siret Talve; Deniz Tasdemir; Xenia Theodotou Schneider; Olivier P. Thomas; Anna Toruńska-Sitarz; Giovanna Cristina Varese; Marlen I. Vasquez. 2021. "The Essentials of Marine Biotechnology." Frontiers in Marine Science 8, no. : 1.
The marine environment is a rich source of biologically active molecules for the treatment of human diseases, especially cancer. The adaptation to unique environmental conditions led marine organisms to evolve different pathways than their terrestrial counterparts, thus producing unique chemicals with a broad diversity and complexity. So far, more than 36,000 compounds have been isolated from marine micro- and macro-organisms including but not limited to fungi, bacteria, microalgae, macroalgae, sponges, corals, mollusks and tunicates, with hundreds of new marine natural products (MNPs) being discovered every year. Marine-based pharmaceuticals have started to impact modern pharmacology and different anti-cancer drugs derived from marine compounds have been approved for clinical use, such as: cytarabine, vidarabine, nelarabine (prodrug of ara-G), fludarabine phosphate (pro-drug of ara-A), trabectedin, eribulin mesylate, brentuximab vedotin, polatuzumab vedotin, enfortumab vedotin, belantamab mafodotin, plitidepsin, and lurbinectedin. This review focuses on the bioactive molecules derived from the marine environment with anticancer activity, discussing their families, origin, structural features and therapeutic use.
Marilia Barreca; Virginia Spanò; Alessandra Montalbano; Mercedes Cueto; Ana R. Díaz Marrero; Irem Deniz; Ayşegül Erdoğan; Lada Lukić Bilela; Corentin Moulin; Elisabeth Taffin-De-Givenchy; Filippo Spriano; Giuseppe Perale; Mohamed Mehiri; Ana Rotter; Olivier P. Thomas; Paola Barraja; Susana P. Gaudêncio; Francesco Bertoni. Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes. Marine Drugs 2020, 18, 619 .
AMA StyleMarilia Barreca, Virginia Spanò, Alessandra Montalbano, Mercedes Cueto, Ana R. Díaz Marrero, Irem Deniz, Ayşegül Erdoğan, Lada Lukić Bilela, Corentin Moulin, Elisabeth Taffin-De-Givenchy, Filippo Spriano, Giuseppe Perale, Mohamed Mehiri, Ana Rotter, Olivier P. Thomas, Paola Barraja, Susana P. Gaudêncio, Francesco Bertoni. Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes. Marine Drugs. 2020; 18 (12):619.
Chicago/Turabian StyleMarilia Barreca; Virginia Spanò; Alessandra Montalbano; Mercedes Cueto; Ana R. Díaz Marrero; Irem Deniz; Ayşegül Erdoğan; Lada Lukić Bilela; Corentin Moulin; Elisabeth Taffin-De-Givenchy; Filippo Spriano; Giuseppe Perale; Mohamed Mehiri; Ana Rotter; Olivier P. Thomas; Paola Barraja; Susana P. Gaudêncio; Francesco Bertoni. 2020. "Marine Anticancer Agents: An Overview with a Particular Focus on Their Chemical Classes." Marine Drugs 18, no. 12: 619.
The chemokine CCL5/RANTES is a versatile inflammatory mediator, which interacts with the receptor CCR5, promoting cancer cell interactions within the tumor microenvironment. Glioblastoma is a highly invasive tumor, in which CCL5 expression correlates with shorter patient survival. Using immunohistochemistry, we identified CCL5 and CCR5 in a series of glioblastoma samples and cells, including glioblastoma stem cells. CCL5 and CCR5 gene expression were significantly higher in a cohort of 38 glioblastoma samples, compared to low-grade glioma and non-cancerous tissues. The in vitro invasion of patients-derived primary glioblastoma cells and glioblastoma stem cells was dependent on CCL5-induced CCR5 signaling and is strongly inhibited by the small molecule CCR5 antagonist maraviroc. Invasion of these cells, which was enhanced when co-cultured with mesenchymal stem cells (MSCs), was inhibited by maraviroc, suggesting that MSCs release CCR5 ligands. In support of this model, we detected CCL5 and CCR5 in MSC monocultures and glioblastoma-associated MSC in tissue sections. We also found CCR5 expressing macrophages were in close proximity to glioblastoma cells. In conclusion, autocrine and paracrine cross-talk in glioblastoma and, in particular, glioblastoma stem cells with its stromal microenvironment, involves CCR5 and CCL5, contributing to glioblastoma invasion, suggesting the CCL5/CCR5 axis as a potential therapeutic target that can be targeted with repositioned drug maraviroc.
Metka Novak; Miha Koprivnikar Krajnc; Barbara Hrastar; Barbara Breznik; Bernarda Majc; Mateja Mlinar; Ana Rotter; Andrej Porčnik; Jernej Mlakar; Katja Stare; Richard G. Pestell; Tamara Lah Turnšek. CCR5-Mediated Signaling is Involved in Invasion of Glioblastoma Cells in Its Microenvironment. International Journal of Molecular Sciences 2020, 21, 4199 .
AMA StyleMetka Novak, Miha Koprivnikar Krajnc, Barbara Hrastar, Barbara Breznik, Bernarda Majc, Mateja Mlinar, Ana Rotter, Andrej Porčnik, Jernej Mlakar, Katja Stare, Richard G. Pestell, Tamara Lah Turnšek. CCR5-Mediated Signaling is Involved in Invasion of Glioblastoma Cells in Its Microenvironment. International Journal of Molecular Sciences. 2020; 21 (12):4199.
Chicago/Turabian StyleMetka Novak; Miha Koprivnikar Krajnc; Barbara Hrastar; Barbara Breznik; Bernarda Majc; Mateja Mlinar; Ana Rotter; Andrej Porčnik; Jernej Mlakar; Katja Stare; Richard G. Pestell; Tamara Lah Turnšek. 2020. "CCR5-Mediated Signaling is Involved in Invasion of Glioblastoma Cells in Its Microenvironment." International Journal of Molecular Sciences 21, no. 12: 4199.
Marine organisms produce a vast diversity of metabolites with biological activities useful for humans, e.g., cytotoxic, antioxidant, anti-microbial, insecticidal, herbicidal, anticancer, pro-osteogenic and pro-regenerative, analgesic, anti-inflammatory, anti-coagulant, cholesterol-lowering, nutritional, photoprotective, horticultural or other beneficial properties. These metabolites could help satisfy the increasing demand for alternative sources of nutraceuticals, pharmaceuticals, cosmeceuticals, food, feed, and novel bio-based products. In addition, marine biomass itself can serve as the source material for the production of various bulk commodities (e.g., biofuels, bioplastics, biomaterials). The sustainable exploitation of marine bio-resources and the development of biomolecules and polymers are also known as the growing field of marine biotechnology. Up to now, over 35,000 natural products have been characterized from marine organisms, but many more are yet to be uncovered, as the vast diversity of biota in the marine systems remains largely unexplored. Since marine biotechnology is still in its infancy, there is a need to create effective, operational, inclusive, sustainable, transnational and transdisciplinary networks with a serious and ambitious commitment for knowledge transfer, training provision, dissemination of best practices and identification of the emerging technological trends through science communication activities. A collaborative (net)work is today compelling to provide innovative solutions and products that can be commercialized to contribute to the circular bioeconomy. This perspective article highlights the importance of establishing such collaborative frameworks using the example of Ocean4Biotech, an Action within the European Cooperation in Science and Technology (COST) that connects all and any stakeholders with an interest in marine biotechnology in Europe and beyond.
Ana Rotter; Ariola Bacu; Michèle Barbier; Francesco Bertoni; Atle M. Bones; M. Leonor Cancela; Jens Carlsson; M. Fátima Carvalho; Marta Cegłowska; Meltem Conk Dalay; Thanos Dailianis; Irem Deniz; Dragana Drakulovic; Arita Dubnika; Hjörleifur Einarsson; Ayşegül Erdoğan; Orhan Tufan Eroldoğan; David Ezra; Stefano Fazi; Richard J. FitzGerald; Laura M. Gargan; Susana P. Gaudêncio; Nadica Ivošević DeNardis; Danijela Joksimovic; Marija Kataržytė; Jonne Kotta; Manolis Mandalakis; Inga Matijošytė; Hanna Mazur-Marzec; Alexia Massa-Gallucci; Mohamed Mehiri; Søren Laurentius Nielsen; Lucie Novoveská; Donata Overlingė; Michelle E. Portman; Krzysztof Pyrc; Céline Rebours; Thorsten Reinsch; Fernando Reyes; Baruch Rinkevich; Johan Robbens; Vita Rudovica; Jerica Sabotič; Ivo Safarik; Siret Talve; Deniz Tasdemir; Xenia Theodotou Schneider; Olivier P. Thomas; Anna Toruńska-Sitarz; Giovanna Cristina Varese; Marlen I. Vasquez. A New Network for the Advancement of Marine Biotechnology in Europe and Beyond. Frontiers in Marine Science 2020, 7, 1 .
AMA StyleAna Rotter, Ariola Bacu, Michèle Barbier, Francesco Bertoni, Atle M. Bones, M. Leonor Cancela, Jens Carlsson, M. Fátima Carvalho, Marta Cegłowska, Meltem Conk Dalay, Thanos Dailianis, Irem Deniz, Dragana Drakulovic, Arita Dubnika, Hjörleifur Einarsson, Ayşegül Erdoğan, Orhan Tufan Eroldoğan, David Ezra, Stefano Fazi, Richard J. FitzGerald, Laura M. Gargan, Susana P. Gaudêncio, Nadica Ivošević DeNardis, Danijela Joksimovic, Marija Kataržytė, Jonne Kotta, Manolis Mandalakis, Inga Matijošytė, Hanna Mazur-Marzec, Alexia Massa-Gallucci, Mohamed Mehiri, Søren Laurentius Nielsen, Lucie Novoveská, Donata Overlingė, Michelle E. Portman, Krzysztof Pyrc, Céline Rebours, Thorsten Reinsch, Fernando Reyes, Baruch Rinkevich, Johan Robbens, Vita Rudovica, Jerica Sabotič, Ivo Safarik, Siret Talve, Deniz Tasdemir, Xenia Theodotou Schneider, Olivier P. Thomas, Anna Toruńska-Sitarz, Giovanna Cristina Varese, Marlen I. Vasquez. A New Network for the Advancement of Marine Biotechnology in Europe and Beyond. Frontiers in Marine Science. 2020; 7 ():1.
Chicago/Turabian StyleAna Rotter; Ariola Bacu; Michèle Barbier; Francesco Bertoni; Atle M. Bones; M. Leonor Cancela; Jens Carlsson; M. Fátima Carvalho; Marta Cegłowska; Meltem Conk Dalay; Thanos Dailianis; Irem Deniz; Dragana Drakulovic; Arita Dubnika; Hjörleifur Einarsson; Ayşegül Erdoğan; Orhan Tufan Eroldoğan; David Ezra; Stefano Fazi; Richard J. FitzGerald; Laura M. Gargan; Susana P. Gaudêncio; Nadica Ivošević DeNardis; Danijela Joksimovic; Marija Kataržytė; Jonne Kotta; Manolis Mandalakis; Inga Matijošytė; Hanna Mazur-Marzec; Alexia Massa-Gallucci; Mohamed Mehiri; Søren Laurentius Nielsen; Lucie Novoveská; Donata Overlingė; Michelle E. Portman; Krzysztof Pyrc; Céline Rebours; Thorsten Reinsch; Fernando Reyes; Baruch Rinkevich; Johan Robbens; Vita Rudovica; Jerica Sabotič; Ivo Safarik; Siret Talve; Deniz Tasdemir; Xenia Theodotou Schneider; Olivier P. Thomas; Anna Toruńska-Sitarz; Giovanna Cristina Varese; Marlen I. Vasquez. 2020. "A New Network for the Advancement of Marine Biotechnology in Europe and Beyond." Frontiers in Marine Science 7, no. : 1.
Wastewater treatment plants (WWTPs) are a focal point for the removal of microplastic (MP) particles before they are discharged into aquatic environments. WWTPs are capable of removing substantial quantities of larger MP particles but are inefficient in removing particles with any one dimension of less than 100 μm, with influents and effluents tending to have similar quantities of these smaller particles. As a single WWTP may release >100 billion MP particles annually, collectively WWTPs are significant contributors to the problem of MP pollution of global surface waters. Currently, there are no policies or regulations requiring the removal of MPs during wastewater treatment, but as concern about MP pollution grows, the potential for wastewater technologies to capture particles before they reach surface waters has begun to attract attention. There are promising technologies in various stages of development that may improve the removal of MP particles from wastewater. Better incentivization could speed up the research, development and adoption of innovative practices. This paper describes the current state of knowledge regarding MPs, wastewater and relevant policies that could influence the development and deployment of new technologies within WWTPs. We review existing technologies for capturing very small MP particles and examine new developments that may have the potential to overcome the shortcomings of existing methods. The types of collaborations needed to encourage and incentivize innovation within the wastewater sector are also discussed, specifically strong partnerships among scientific and engineering researchers, industry stakeholders, and policy decision makers.
Shirra Freeman; Andy M. Booth; Isam Sabbah; Rachel Tiller; Jan Dierking; Katja Klun; Ana Rotter; Eric Ben-David; Jamileh Javidpour; Dror L. Angel. Between source and sea: The role of wastewater treatment in reducing marine microplastics. Journal of Environmental Management 2020, 266, 110642 .
AMA StyleShirra Freeman, Andy M. Booth, Isam Sabbah, Rachel Tiller, Jan Dierking, Katja Klun, Ana Rotter, Eric Ben-David, Jamileh Javidpour, Dror L. Angel. Between source and sea: The role of wastewater treatment in reducing marine microplastics. Journal of Environmental Management. 2020; 266 ():110642.
Chicago/Turabian StyleShirra Freeman; Andy M. Booth; Isam Sabbah; Rachel Tiller; Jan Dierking; Katja Klun; Ana Rotter; Eric Ben-David; Jamileh Javidpour; Dror L. Angel. 2020. "Between source and sea: The role of wastewater treatment in reducing marine microplastics." Journal of Environmental Management 266, no. : 110642.
For mitigation of the effects of pollution, the media, policy makers and, in turn, the scientific community and industry each provide contributions through development of a sense of urgency, and with guidelines and solutions. For non-indigenous species (NIS) that can frequently have negative impacts on the native biota, this is often conveyed in an emotive way to the general public, who are typically keen to help and to get personally involved. This might be through organization of cleaning campaigns, influence on the media, or collaboration with scientists, to inform them of the local presence and abundance of NIS. Alternatively, they might proactively develop technological solutions themselves. To assess the current state of affairs, we reviewed the presence and effects of NIS in the Mediterranean Sea. As so often, any well-planned and successful activity is directly linked to financing, or a lack thereof, and this leads to sometimes untargeted and sporadic measures that are developed within a project or over a limited timeframe, without any sustainability measures. Therefore, we also assessed the activities and strategies that have been financed in this area of NIS mitigation. Based on this review of the presence and impact of NIS, and previous and ongoing activities, we propose a new paradigm to mitigate such pollution: the 8Rs model (i.e., Recognize, Reduce, Replace, Reuse, Recycle, Recover/Restore, Remove, and Regulate). This model extends from the more traditional 3Rs model (i.e., Reduce, Reuse, and Recycle) that is often used and promoted for innovative waste management strategies. Importantly, the 8Rs model can be applied sequentially, for either prevention of NIS introduction, or preparation of mitigation measures. The 8Rs model was constructed based on Mediterranean NIS, although we believe it can be applied to other sources of pollution and other geographic areas. Importantly, the 8Rs model represents a general framework to organize and categorize future pollution mitigation strategies. This approach is essential for development of any action plan to influence the administrative and financial decision makers who essentially enable these activities, and therefore who have important roles in the guarantee of sustainability of these actions, and the creation of innovative societies.
Ana Rotter; Katja Klun; Janja Francé; Patricija Mozetič; Martina Orlando-Bonaca. Non-indigenous Species in the Mediterranean Sea: Turning From Pest to Source by Developing the 8Rs Model, a New Paradigm in Pollution Mitigation. Frontiers in Marine Science 2020, 7, 1 .
AMA StyleAna Rotter, Katja Klun, Janja Francé, Patricija Mozetič, Martina Orlando-Bonaca. Non-indigenous Species in the Mediterranean Sea: Turning From Pest to Source by Developing the 8Rs Model, a New Paradigm in Pollution Mitigation. Frontiers in Marine Science. 2020; 7 ():1.
Chicago/Turabian StyleAna Rotter; Katja Klun; Janja Francé; Patricija Mozetič; Martina Orlando-Bonaca. 2020. "Non-indigenous Species in the Mediterranean Sea: Turning From Pest to Source by Developing the 8Rs Model, a New Paradigm in Pollution Mitigation." Frontiers in Marine Science 7, no. : 1.
Agricultural production is predicted to double during the next century. To ensure food security in response to global population growth is a challenge and will require strategies that mitigate associated environmental damage in ways consistent with United Nation’s Sustainable Development Goals. One possible approach is to utilize organic fertilizers from marine sources to improve soil structure by enhancing activities of soil organisms and restoring essential plant nutrients to the soil. Here we identify opportunities to develop organic fertilizers from two types of materials of marine origin: seagrass wrack and jellyfish biomass. Seagrass wrack often occurs as undesirable waste material on beaches. In many coastal areas around the world jellyfish bloom presents a nuisance because of negative impacts on marine ecosystem productivity. Several investigations have reported that organic fertilizers produced from seagrass and jellyfish could enhance coastal ecosystem services by reducing pollution, and by improving soil health and quality. Recent research indicates that seagrass litter improves soil water holding capacity and the nutritional value of crops; moreover, it can be used as multi-functional fertilizer, due to its content of valuable macro- and microelements. The application of jellyfish fertilizer increases soil contents of organic matter, nitrogen, phosphorus and potassium and enhances the growth and survival of seedlings significantly. In this overview we describe novel approaches regarding the utilization of seagrass and jellyfish as sources of fertilizer, and experimental studies on the influences of marine organic fertilizers on soil restoration, and implications for coastal management.
Iraj Emadodin; Thorsten Reinsch; Ana Rotter; Martina Orlando-Bonaca; Friedhelm Taube; Jamileh Javidpour. A perspective on the potential of using marine organic fertilizers for the sustainable management of coastal ecosystem services. Environmental Sustainability 2020, 3, 105 -115.
AMA StyleIraj Emadodin, Thorsten Reinsch, Ana Rotter, Martina Orlando-Bonaca, Friedhelm Taube, Jamileh Javidpour. A perspective on the potential of using marine organic fertilizers for the sustainable management of coastal ecosystem services. Environmental Sustainability. 2020; 3 (1):105-115.
Chicago/Turabian StyleIraj Emadodin; Thorsten Reinsch; Ana Rotter; Martina Orlando-Bonaca; Friedhelm Taube; Jamileh Javidpour. 2020. "A perspective on the potential of using marine organic fertilizers for the sustainable management of coastal ecosystem services." Environmental Sustainability 3, no. 1: 105-115.
An inventory of phytoplankton diversity in 12 Adriatic ports was performed with the port baseline survey. Particular emphasis was put on the detection of harmful aquatic organisms and pathogens (HAOP) because of their negative impact on ecosystem, human health, and the economy. Phytoplanktonic HAOP are identified as species, either native or non-indigenous (NIS), which can trigger harmful algal blooms (HAB). A list of 691 taxa was prepared, and among them 52 were classified as HAB and five as NIS. Records of toxigenic NIS (Pseudo-nitzschia multistriata, Ostreopsis species including O. cf. ovata) indicate that the intrusion of non-native invasive phytoplankton species has already occurred in some Adriatic ports. The seasonal occurrence and abundance of HAOP offers a solid baseline for a monitoring design in ports in order to prevent ballast water uptake and possible expansion of HAOP outside their native region.
Patricija Mozetič; Monica Cangini; Janja Francé; Mauro Bastianini; Fabrizio Bernardi Aubry; Mia Bužančić; Marina Cabrini; Federica Cerino; Marijeta Čalić; Raffaele D'Adamo; Dragana Drakulović; Stefania Finotto; Daniela Fornasaro; Federica Grilli; Romina Kraus; Nataša Kužat; Daniela Marić Pfannkuchen; Živana Ninčević Gladan; Marinella Pompei; Ana Rotter; Irene Servadei; Sanda Skejić. Phytoplankton diversity in Adriatic ports: Lessons from the port baseline survey for the management of harmful algal species. Marine Pollution Bulletin 2019, 147, 117 -132.
AMA StylePatricija Mozetič, Monica Cangini, Janja Francé, Mauro Bastianini, Fabrizio Bernardi Aubry, Mia Bužančić, Marina Cabrini, Federica Cerino, Marijeta Čalić, Raffaele D'Adamo, Dragana Drakulović, Stefania Finotto, Daniela Fornasaro, Federica Grilli, Romina Kraus, Nataša Kužat, Daniela Marić Pfannkuchen, Živana Ninčević Gladan, Marinella Pompei, Ana Rotter, Irene Servadei, Sanda Skejić. Phytoplankton diversity in Adriatic ports: Lessons from the port baseline survey for the management of harmful algal species. Marine Pollution Bulletin. 2019; 147 ():117-132.
Chicago/Turabian StylePatricija Mozetič; Monica Cangini; Janja Francé; Mauro Bastianini; Fabrizio Bernardi Aubry; Mia Bužančić; Marina Cabrini; Federica Cerino; Marijeta Čalić; Raffaele D'Adamo; Dragana Drakulović; Stefania Finotto; Daniela Fornasaro; Federica Grilli; Romina Kraus; Nataša Kužat; Daniela Marić Pfannkuchen; Živana Ninčević Gladan; Marinella Pompei; Ana Rotter; Irene Servadei; Sanda Skejić. 2019. "Phytoplankton diversity in Adriatic ports: Lessons from the port baseline survey for the management of harmful algal species." Marine Pollution Bulletin 147, no. : 117-132.
The oceans cover nearly three-quarters of the Earth’s surface and produce vast quantities of sea spray aerosols (SSA). Studies have shown that due to ocean biology SSA particles are comprised of much more than just sea salt and often include proteins, lipids, sugars, viruses, and bacteria. In this study, we show for the first time that a diverse array of microbial enzymes (protease, lipases, and alkaline phosphatase) are transferred from the ocean into the atmosphere and often become even more active with measured activities in SSA particles that are 1–2 orders of magnitude higher than those in bulk seawater. We hypothesize that these enzymatic reactions are enhanced in the interfacial environment of droplets and aerosols that can dynamically modify surface chemical species and properties. Simulations reveal that enzyme-containing SSA particles can rapidly coagulate with other preexisting aerosols, thus transferring the impact of enzyme reactions to a broad range of marine aerosols. These biotic reaction pathways are expected to profoundly change the composition of marine aerosols, particularly at the interface, and thus will impact cloud properties in marine environments. Future studies are needed to determine how photochemistry, changing ocean conditions in a warming climate, and other external factors will influence the activities of these enzymes and their impact on the composition of the marine atmosphere.
Francesca Malfatti; Christopher Lee; Tinkara Tinta; Matthew A. Pendergraft; Mauro Celussi; Yanyan Zhou; Camille M. Sultana; Ana Rotter; Jessica L. Axson; Douglas B. Collins; Mitchell V. Santander; Alma L. Anides Morales; Lihini I. Aluwihare; Nicole Riemer; Vicki H. Grassian; Farooq Azam; Kimberly A. Prather. Detection of Active Microbial Enzymes in Nascent Sea Spray Aerosol: Implications for Atmospheric Chemistry and Climate. Environmental Science & Technology Letters 2019, 6, 171 -177.
AMA StyleFrancesca Malfatti, Christopher Lee, Tinkara Tinta, Matthew A. Pendergraft, Mauro Celussi, Yanyan Zhou, Camille M. Sultana, Ana Rotter, Jessica L. Axson, Douglas B. Collins, Mitchell V. Santander, Alma L. Anides Morales, Lihini I. Aluwihare, Nicole Riemer, Vicki H. Grassian, Farooq Azam, Kimberly A. Prather. Detection of Active Microbial Enzymes in Nascent Sea Spray Aerosol: Implications for Atmospheric Chemistry and Climate. Environmental Science & Technology Letters. 2019; 6 (3):171-177.
Chicago/Turabian StyleFrancesca Malfatti; Christopher Lee; Tinkara Tinta; Matthew A. Pendergraft; Mauro Celussi; Yanyan Zhou; Camille M. Sultana; Ana Rotter; Jessica L. Axson; Douglas B. Collins; Mitchell V. Santander; Alma L. Anides Morales; Lihini I. Aluwihare; Nicole Riemer; Vicki H. Grassian; Farooq Azam; Kimberly A. Prather. 2019. "Detection of Active Microbial Enzymes in Nascent Sea Spray Aerosol: Implications for Atmospheric Chemistry and Climate." Environmental Science & Technology Letters 6, no. 3: 171-177.
The cyanobacterial pentapeptide nodularin (NOD), mainly produced by genus Nodularia, is a potent inhibitor of protein phosphatases PPA1 and PPA2, and causes animal mortality. The few studies available indicate that NOD is a potential non-genotoxic carcinogen. In the present study we evaluated NOD (0.01, 0.1 and 1 μg/ml) genotoxic activity in human hepatoma (HepG2) cells with the comet, γH2AX and cytokinesis block micronucleus cytome assays. In addition, induction of oxidative stress was studied. Moreover changes in the expression of selected genes from the P53 pathway, involved in the response to DNA damage (P53, GADD45α, CDKN1A, MDM2), apoptosis (BAX, BCL2) and oxidative stress (GPX1, GSR, GCLC, CAT, SOD1) were determined using qPCR. Non-cytotoxic concentrations induced time and dose dependant increase in reactive oxygen species (ROS) production and substantially increased the formation of oxidative DNA damage. In addition, elevated formation of micronuclei was detected. For the first time it has been shown that NOD deregulated the mRNA level of DNA damage (CDKN1A, GADD45α) and oxidative stress (GPX1, GSR, GCLC, CAT and SOD1) responsive genes and anti-apoptotic gene BCL2. Our results provide new evidence that NOD genotoxic effects are mediated through ROS production, already at low environmentally relevant concentrations.
A. Štern; A. Rotter; M. Novak; M. Filipič; B. Žegura. Genotoxic effects of the cyanobacterial pentapeptide nodularin in HepG2 cells. Food and Chemical Toxicology 2018, 124, 349 -358.
AMA StyleA. Štern, A. Rotter, M. Novak, M. Filipič, B. Žegura. Genotoxic effects of the cyanobacterial pentapeptide nodularin in HepG2 cells. Food and Chemical Toxicology. 2018; 124 ():349-358.
Chicago/Turabian StyleA. Štern; A. Rotter; M. Novak; M. Filipič; B. Žegura. 2018. "Genotoxic effects of the cyanobacterial pentapeptide nodularin in HepG2 cells." Food and Chemical Toxicology 124, no. : 349-358.
Human papillomavirus (HPV) is an important etiological factor in oropharyngeal squamous cell carcinoma (SCC). Compared to HPV-negative tumors, HPV-positive oropharyngeal SCC has shown a better response to nonsurgical treatments. In this study, we determined the dose-modifying factors for HPV-positive tumors with single-dose irradiation, with or without low radiosensitizing doses of cisplatin.In vitro, we determined an increased radiosensitivity of HPV-positive SCC, which might be a consequence of HPV-induced changes in the cell cycle regulation and DNA damage response, leading to increased cell death. Additionally, compared to HPV-negative tumors, 30% higher radiosensitivity of HPV-positive tumors was determined by tumor growth delay monitoring in immunodeficient mice in vivo. Concurrent cisplatin treatment had an additive effect in both HPV-negative and HPV-positive tumors, resulting in 20% better response in HPV-positive tumors than in HPV-negative tumors.
Ajda Prevc; Simona Kranjc; Maja Čemažar; Vesna Todorovic; Bojana Žegura; Matjaž Novak; Metka Filipič; Margareta Strojan Fležar; Irena Srebotnik Kirbiš; Ana Rotter; Andreja Brozic; Martina Niksic Zakelj; Mario Poljak; Lea Hošnjak; Blaž Grošelj; Primož Strojan; Gregor Sersa. Dose-Modifying Factor of Radiation Therapy with Concurrent Cisplatin Treatment in HPV-Positive Squamous Cell Carcinoma: A Preclinical Study. Radiation Research 2018, 189, 644 -651.
AMA StyleAjda Prevc, Simona Kranjc, Maja Čemažar, Vesna Todorovic, Bojana Žegura, Matjaž Novak, Metka Filipič, Margareta Strojan Fležar, Irena Srebotnik Kirbiš, Ana Rotter, Andreja Brozic, Martina Niksic Zakelj, Mario Poljak, Lea Hošnjak, Blaž Grošelj, Primož Strojan, Gregor Sersa. Dose-Modifying Factor of Radiation Therapy with Concurrent Cisplatin Treatment in HPV-Positive Squamous Cell Carcinoma: A Preclinical Study. Radiation Research. 2018; 189 (6):644-651.
Chicago/Turabian StyleAjda Prevc; Simona Kranjc; Maja Čemažar; Vesna Todorovic; Bojana Žegura; Matjaž Novak; Metka Filipič; Margareta Strojan Fležar; Irena Srebotnik Kirbiš; Ana Rotter; Andreja Brozic; Martina Niksic Zakelj; Mario Poljak; Lea Hošnjak; Blaž Grošelj; Primož Strojan; Gregor Sersa. 2018. "Dose-Modifying Factor of Radiation Therapy with Concurrent Cisplatin Treatment in HPV-Positive Squamous Cell Carcinoma: A Preclinical Study." Radiation Research 189, no. 6: 644-651.
Canopy-forming algal species, like those of the genus Cystoseira, are known to play an important role as ecosystems-engineers. Under key anthropogenic stressors (e.g. nutrient enrichment and urbanization), these species are replaced by persistent and smaller taxa, defined as turf-forming algae. The scope of this work was to search for shifts from canopy-forming to turf-forming taxa, changes in macrophytes spatial and seasonal diversity, and trends in macrophytes biodiversity on the infralittoral rocky bottom of the northern Adriatic Sea, during the period from 2006 to 2016. At many sampling sites a decline in the proportion of canopy-forming algae (Cystoseira spp. and Halopithys incurva) was observed until 2012, followed by a slight recovery in 2016. Moreover, the Shannon diversity index calculated for all upper-infralittoral macrophytes increased from 2006 to 2012, then decreased in the following two years, with a new increase from 2015. Despite the assessed fluctuations in the status of macroalgae, we can conclude that there are no visible signs (yet) of degradation of the benthic vegetation.
Martina Orlando-Bonaca; Ana Rotter. Any signs of replacement of canopy-forming algae by turf-forming algae in the northern Adriatic Sea? Ecological Indicators 2018, 87, 272 -284.
AMA StyleMartina Orlando-Bonaca, Ana Rotter. Any signs of replacement of canopy-forming algae by turf-forming algae in the northern Adriatic Sea? Ecological Indicators. 2018; 87 ():272-284.
Chicago/Turabian StyleMartina Orlando-Bonaca; Ana Rotter. 2018. "Any signs of replacement of canopy-forming algae by turf-forming algae in the northern Adriatic Sea?" Ecological Indicators 87, no. : 272-284.
Aquatic biomonitoring has become an essential task in Europe and many other regions as a consequence of strong anthropogenic pressures affecting the health of lakes, rivers, oceans and groundwater. A typical assessment of the environmental quality status, such as it is required by European but also North American and other legislation, relies on matching the composition of assemblages of organisms identified using morphological criteria present in aquatic ecosystems to those expected in the absence of anthropogenic pressures. Through decade-long and difficult intercalibration exercises among networks of regulators and scientists in European countries, a pragmatic biomonitoring approach was developed and adopted, which now produces invaluable information. Nonetheless, this approach is based on several hundred different protocols, making it susceptible to issues with comparability, scale and resolution. Furthermore, data acquisition is often slow due to a lack of taxonomic experts for many taxa and regions and time-consuming morphological identification of organisms. High-throughput genetic screening methods such as (e)DNA metabarcoding have been proposed as a possible solution to these shortcomings. Such “next-generation biomonitoring”, also termed “biomonitoring 2.0”, has many advantages over the traditional approach in terms of speed, comparability and costs. It also creates the potential to include new bioindicators and thereby further improves the assessment of aquatic ecosystem health. However, several major conceptual and technological challenges still hinder its implementation into legal and regulatory frameworks. Academic scientists sometimes tend to overlook legal or socioeconomic constraints, which regulators have to consider on a regular basis. Moreover, quantification of species abundance or biomass remains a significant bottleneck to releasing the full potential of these approaches. Here, we highlight the main challenges for next-generation aquatic biomonitoring and outline principles and good practices to address these with an emphasis on bridging traditional disciplinary boundaries between academics, regulators, stakeholders and industry.
Florian Leese; Agnès Bouchez; Kessy Abarenkov; Florian Altermatt; Angel Borja; Kat Bruce; Torbjørn Ekrem; Fedor Čiampor; Zuzana Čiamporová-Zaťovičová; Filipe O. Costa; Sofia Duarte; Vasco Elbrecht; Diego Fontaneto; Alain Franc; Matthias F. Geiger; Daniel Hering; Maria Kahlert; Belma Kalamujić Stroil; Martyn Kelly; Emre Keskin; Igor Liska; Patricia Mergen; Kristian Meissner; Jan Pawlowski; Lyubomir Penev; Yorick Reyjol; Ana Rotter; Dirk Steinke; Bas van der Wal; Simon Vitecek; Jonas Zimmermann; Alexander M. Weigand. Why We Need Sustainable Networks Bridging Countries, Disciplines, Cultures and Generations for Aquatic Biomonitoring 2.0: A Perspective Derived From the DNAqua-Net COST Action. Advances in Ecological Research 2018, 63 -99.
AMA StyleFlorian Leese, Agnès Bouchez, Kessy Abarenkov, Florian Altermatt, Angel Borja, Kat Bruce, Torbjørn Ekrem, Fedor Čiampor, Zuzana Čiamporová-Zaťovičová, Filipe O. Costa, Sofia Duarte, Vasco Elbrecht, Diego Fontaneto, Alain Franc, Matthias F. Geiger, Daniel Hering, Maria Kahlert, Belma Kalamujić Stroil, Martyn Kelly, Emre Keskin, Igor Liska, Patricia Mergen, Kristian Meissner, Jan Pawlowski, Lyubomir Penev, Yorick Reyjol, Ana Rotter, Dirk Steinke, Bas van der Wal, Simon Vitecek, Jonas Zimmermann, Alexander M. Weigand. Why We Need Sustainable Networks Bridging Countries, Disciplines, Cultures and Generations for Aquatic Biomonitoring 2.0: A Perspective Derived From the DNAqua-Net COST Action. Advances in Ecological Research. 2018; ():63-99.
Chicago/Turabian StyleFlorian Leese; Agnès Bouchez; Kessy Abarenkov; Florian Altermatt; Angel Borja; Kat Bruce; Torbjørn Ekrem; Fedor Čiampor; Zuzana Čiamporová-Zaťovičová; Filipe O. Costa; Sofia Duarte; Vasco Elbrecht; Diego Fontaneto; Alain Franc; Matthias F. Geiger; Daniel Hering; Maria Kahlert; Belma Kalamujić Stroil; Martyn Kelly; Emre Keskin; Igor Liska; Patricia Mergen; Kristian Meissner; Jan Pawlowski; Lyubomir Penev; Yorick Reyjol; Ana Rotter; Dirk Steinke; Bas van der Wal; Simon Vitecek; Jonas Zimmermann; Alexander M. Weigand. 2018. "Why We Need Sustainable Networks Bridging Countries, Disciplines, Cultures and Generations for Aquatic Biomonitoring 2.0: A Perspective Derived From the DNAqua-Net COST Action." Advances in Ecological Research , no. : 63-99.
Bois noir (BN) is the most widespread European grapevine yellows disease caused by ‘Candidatus Phytoplasma solani’. Although our knowledge of the mechanisms of interactions of this pathogenic bacteria with host is largely unknown, the plant-pathogen system of BN is commonly used as a model system for studying grapevine yellows diseases. We applied here a conceptual model of general plant pathology – a disease triangle for describing interactions among the host plant, the pathogen and the environment. We generated a proof-of-concept statistical model for disease triangle using original experimental data and different statistical and data mining approaches for a selected system of ‘Ca. P. solani’ infection of cv. ‘Chardonnay’ grapevine plants. We monitored individual plants from a single vineyard over a period of six years. Phytoplasma content, the expression of 21 selected grapevine genes and environmental conditions were recorded and related to disease severity. Our model predicts that in described conditions BN is a function of the expression of grapevine gene VvDMR6, summer rainfall and abundance of ‘Ca. P. solani’. The greatest impact among elements of the disease triangle is attributed to the pathogen, and is independent of the pathogen titer. We showed that this first de facto representation of the disease triangle is useful for showing disease dynamics over several years and could be applied to other plant-pathogen systems. The overall results of this study will contribute to understanding of ‘Ca. P. solani’ biology and its interactions with grapevine host.
Ana Rotter; Petra Nikolić; Neža Turnšek; Polona Kogovšek; Andrej Blejec; Kristina Gruden; Marina Dermastia. Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field. European Journal of Plant Pathology 2017, 150, 653 -668.
AMA StyleAna Rotter, Petra Nikolić, Neža Turnšek, Polona Kogovšek, Andrej Blejec, Kristina Gruden, Marina Dermastia. Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field. European Journal of Plant Pathology. 2017; 150 (3):653-668.
Chicago/Turabian StyleAna Rotter; Petra Nikolić; Neža Turnšek; Polona Kogovšek; Andrej Blejec; Kristina Gruden; Marina Dermastia. 2017. "Statistical modeling of long-term grapevine response to ‘Candidatus Phytoplasma solani’ infection in the field." European Journal of Plant Pathology 150, no. 3: 653-668.
Quantitative molecular biology remains a challenge for researchers due to inconsistent approaches for control of errors in the final results. Due to several factors that can influence the final result, quantitative analysis and interpretation of qPCR data are still not trivial. Together with the development of high-throughput qPCR platforms, there is a need for a tool allowing for robust, reliable and fast nucleic acid quantification. We have developed “quantGenius” (http://quantgenius.nib.si), an open-access web application for a reliable qPCR-based quantification of nucleic acids. The quantGenius workflow interactively guides the user through data import, quality control (QC) and calculation steps. The input is machine- and chemistry–independent. Quantification is performed using the standard curve approach, with normalization to one or several reference genes. The special feature of the application is the implementation of user-guided QC-based decision support system, based on qPCR standards, that takes into account pipetting errors, assay amplification efficiencies, limits of detection and quantification of the assays as well as the control of PCR inhibition in individual samples. The intermediate calculations and final results are exportable in a data matrix suitable for further statistical analysis or visualization. We additionally compare the most important features of quantGenius with similar advanced software tools and illustrate the importance of proper QC system in the analysis of qPCR data in two use cases. To our knowledge, quantGenius is the only qPCR data analysis tool that integrates QC-based decision support and will help scientists to obtain reliable results which are the basis for biologically meaningful data interpretation.
Špela Baebler; Miha Svalina; Marko Petek; Katja Stare; Ana Rotter; Maruša Pompe-Novak; Kristina Gruden. quantGenius: implementation of a decision support system for qPCR-based gene quantification. BMC Bioinformatics 2017, 18, 1 -11.
AMA StyleŠpela Baebler, Miha Svalina, Marko Petek, Katja Stare, Ana Rotter, Maruša Pompe-Novak, Kristina Gruden. quantGenius: implementation of a decision support system for qPCR-based gene quantification. BMC Bioinformatics. 2017; 18 (1):1-11.
Chicago/Turabian StyleŠpela Baebler; Miha Svalina; Marko Petek; Katja Stare; Ana Rotter; Maruša Pompe-Novak; Kristina Gruden. 2017. "quantGenius: implementation of a decision support system for qPCR-based gene quantification." BMC Bioinformatics 18, no. 1: 1-11.
Glioblastoma multiforme are an aggressive form of brain tumors that are characterized by distinct invasion of single glioblastoma cells, which infiltrate the brain parenchyma. This appears to be stimulated by the communication between cancer and stromal cells. Mesenchymal stem cells (MSCs) are part of the glioblastoma microenvironment, and their 'cross-talk' with glioblastoma cells is still poorly understood. Here, we examined the effects of bone marrow-derived MSCs on two different established glioblastoma cell lines U87 and U373. We focused on mutual effects of direct MSC/glioblastoma contact on cellular invasion in three-dimensional invasion assays in vitro and in a zebrafish embryo model in vivo. This is the first demonstration of glioblastoma cell-type-specific responses to MSCs in direct glioblastoma co-cultures, where MSCs inhibited the invasion of U87 cells and enhanced the invasion of U373. Inversely, direct cross-talk between MSCs and both of glioblastoma cell lines enhanced MSC motility. MSC-enhanced invasion of U373 cells was assisted by overexpression of proteases cathepsin B, calpain1, uPA/uPAR, MMP-2, -9 and -14, and increased activities of some of these proteases, as determined by the effects of their selective inhibitors on invasion. In contrast, these proteases had no effect on U87 cell invasion under MSC co-culturing. Finally, we identified differentially expressed genes, in U87 and U373 cells that could explain different response of these cell lines to MSCs. In conclusion, we demonstrated that MSC/glioblastoma cross-talk is different in the two glioblastoma cell phenotypes, which contributes to tumor heterogeneity.
Barbara Breznik; Helena Motaln; Miloš Vittori; Ana Rotter; Tamara Lah Turnšek. Mesenchymal stem cells differentially affect the invasion of distinct glioblastoma cell lines. Oncotarget 2017, 8, 25482 -25499.
AMA StyleBarbara Breznik, Helena Motaln, Miloš Vittori, Ana Rotter, Tamara Lah Turnšek. Mesenchymal stem cells differentially affect the invasion of distinct glioblastoma cell lines. Oncotarget. 2017; 8 (15):25482-25499.
Chicago/Turabian StyleBarbara Breznik; Helena Motaln; Miloš Vittori; Ana Rotter; Tamara Lah Turnšek. 2017. "Mesenchymal stem cells differentially affect the invasion of distinct glioblastoma cell lines." Oncotarget 8, no. 15: 25482-25499.