Background: Clostridioides (Clostridium) difficile is the most common nosocomial pathogen and antibiotic-related diarrhea in health-care facilities. Over the last few years, there was an increase in the incidence rate of C. difficile infection cases in Slovakia. In this study, the phenotypic (toxigenicity, antimicrobial susceptibility) and genotypic (PCR ribotypes, genes for binary toxins) patterns of C. difficile isolates from patients with CDI were analyzed, from July to August 2016, taken from hospitals in the Horne Povazie region of northern Slovakia. The aim of the study was also to identify hypervirulent strains (e.g., the presence of RT027 or RT176). Methods: The retrospective analysis of biological samples suspected of CDI were analyzed by GDH, anaerobic culture, enzyme immunoassay on toxins A/B, multiplex “real-time” PCR and PCR capillary-based electrophoresis ribotyping, and by MALDI TOF MS. Results: C. difficile isolates (n = 44) were identified by PCR ribotyping, which revealed five different ribotypes (RT001, 011, 017, 081, 176). The presence of hypervirulent RT027 was not identified. The C. difficile isolates (RT001, 011, 081, 176) were susceptible to metronidazole and vancomycin. One isolate RT017 had reduced susceptibility to vancomycin. A statistically significant difference between the most prevalent PCR ribotypes, RT001 and RT176, regarding variables such as albumin, CRP, creatinine, the length of hospitalization (p = 0.175), and glomerular filtration (p = 0.05) was not found. Conclusion: The results of PCR capillary-based electrophoresis ribotyping in the studied samples showed a high prevalence of RT176 and 001.

Human resources are a determining factor in ensuring business prosperity. Skilled, educated and motivated employees are a source of competitive advantage for businesses and they are an active part of the business. They secure the production of goods, the provision of services, the fulfillment of objectives and the definition of strategies, how to achieve these goals. Recruiting and selecting of qualified employees is a challenging process at the time of globalization. It is particularly important for candidates to have a combination of soft skills, technical and digital know-how. The company has to deal with the issue of occupation of jobs by suitable employees and at the same time the conditions have to be created in order to maintain and stabilize employees. They must constantly strive to maintain, respectively increasing employee satisfaction. The paper deals with the issue of recruitment and selection of employees as part of the personnel policy of the company, based on the knowledge that the inefficient process of filling vacancies can negatively affect the course of other personnel activities, but also the operation of the whole company and its profitability. The paper includes an analysis of the process of filling jobs in the company. In the paper an expert interview and survey is carried out, based on which effective approaches in recruiting and selecting employees in the selected enterprise are proposed.

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