Systemic seed treatment uptake was investigated in seeds and seedlings using fluorescent tracers to mimic systemic agrochemicals. Soybean was used as the model as soybean has the permeable seed coat characteristic to both charged and noncharged molecules. The purpose of the paper is to (1) screen 32 fluorescent tracers and then use optimal tracers for seed and seedling uptake, (2) investigate varietal differences in seed uptake, (3) examine the distribution of tracer uptake into 14-day-old seedlings, and (4) study the relationship between seed treatment lipophilicity, measured as log P on seed and root uptake. The major chemical families that displayed both seed and seedling uptake were coumarins and xanthenes. Seed uptake of coumarin 120 ranged from 1.1% to 4.8% of the applied seed treatment tracer from 15 yellow-seeded varieties. Rhodamine B, a xanthene compound uptake in seedlings, showed translocation from the applied seed treatment to all seedling tissues. Most of the tracer was measured in the hypocotyl and root, with lesser amounts in the epicotyl and true leaves. Log P is well documented in the literature to model systemic uptake by roots, but log P of the tracers were not related to seed uptake.

The two major concerns of sustainable agriculture are safeguarding food security and reducing greenhouse gas emissions. Studies on the performance of strip-till with controlled-release blended fertilizer on rainfed maize grain yield, greenhouse gas emissions, and net ecosystem economic budget are scarce in the hilly region of northeast China. In this study, the differences between strip-till (RST) and conventional ridge cropping (CP), straw off-field no-tillage (NT), and no-tillage with straw mulching (RNT) were comparatively investigated in the conventional fertilizer (Sd) mode. And meanwhile, four fertilization modes were also set up under strip-till (RST): conventional fertilization (Sd), controlled-release nitrogen fertilizer blended with normal urea 3:7 (30%Cr), controlled-release nitrogen fertilizer blended with normal urea 5:5 (50%Cr), and no-nitrogen fertilization. We analyzed maize yield, greenhouse gas emissions (GHG), greenhouse gas intensity (GHGI), net income and net ecosystem economic budget (NEEB) for different treatments. The results showed that, under conventional fertilizer (Sd) mode, the maize yield of RST increased by 4.2%, 6.0% and 7.2% compared with NT, CP and RNT and the net income increased by 7.0%, 9.7% and 10.0%, respectively. Compared with CP and NT, although RST increased CO₂ and N₂O emissions, the GHGI of RST was not significantly different from CP and NT, and was 8.0% lower than that of RNT. The NEEB of RST increased by 6.8%, 9.7% and 11.0%, respectively, compared with NT, CP and RNT. Under strip-till, compared with 30%Cr and Sd, the yield of 50%Cr increased by 4.0% and 9.2% and the net income increased by 3.5% and 6.9%, respectively. There was no significant difference in GHGI between 50%Cr and 30%Cr, and 50%Cr decreased by 10.4% compared with Sd. The NEEB of 50%Cr increased by 3.8% and 7.4% compared to 30%Cr and Sd. Strip-till combines controlled-release nitrogen fertilizer blended with normal urea 5:5 (50%Cr) and can be applied as a sustainable strategy to improve the economic efficiency of maize and reduce environmental costs in the hilly region of northeast China.

Potato Fusarium Wilt is a soil-borne fungal disease that can seriously harm potatoes throughout their growth period and occurs at different degrees in major potato-producing areas in China. To reduce the use of chemical agents and improve the effect of biocontrol agents, the inhibitory effects of the fermentation broth of Bacillus subtilis ZWZ-19 (B) and Trichoderma asperellum PT-29 (T) on Fusarium oxysporum were compared under single-culture and co-culture conditions. Furthermore, metabolomic analysis of the fermentation broths was conducted. The results showed that the inhibitory effect of the co-culture fermentation broth with an inoculation ratio of 1:1 (B1T1) was better than that of the separately cultured fermentation broths and had the best control effect in a potted experiment. Using LC-MS analysis, 134 metabolites were determined and classified into different types of amino acids. Furthermore, 10 metabolic pathways had the most significant variations, and 12 were related to amino acid metabolism in the KEGG analysis. A correlation analysis of the 79 differential metabolites generated through the comprehensive comparison between B, T, and B1T1 was conducted, and the results showed that highly abundant amino acids in B1T1 were correlated with amino acids in B, but not in T.

As the third-largest maize-producing province in China and a typical arid and semi-arid region, quantitatively evaluating the carbon and nitrogen footprints of maize production and their dominant factors is of great significance in guiding the high-yield, low-carbon, and sustainable development of maize production in the Inner Mongolia Autonomous Region. This study quantitatively evaluated the interannual evolution characteristics of the carbon and nitrogen footprints in maize production and their dominant factors in Inner Mongolia from 2003 to 2022 based on statistical yearbook data and emission parameter models. The results showed that from 2003 to 2022, the maize planting area, yield, and total yield in Inner Mongolia all increased, with an average annual increase of 97 kg ha⁻¹ in yield and 1.23 × 10⁶ Mg in total yield. The carbon and nitrogen footprints of Inner Mongolia maize production over the past 20 years had overall decreasing trends, while the nitrogen fertilizer bias productivity, net ecosystem carbon balance, and sustainability index had increasing trends. Carbon footprint reduced by an average of 5.2 kg CO₂ eq Mg⁻¹ per year, and nitrogen footprint reduced by an average of 0.21 g N eq kg⁻¹ per year. Currently, the transportation and production of fertilizer and field application of N fertilizer are the main controlling factors of GHG emissions from maize production. NO₃⁻-N, NH₄⁺-N leaching, and NH₃ volatilization from field application of N fertilizer are the main sources of reactive N losses. The application of simplified processes, such as phased regulation of nitrogen and controlled-release fertilizers, as well as conservation tillage, have broad prospects for emission reduction in maize production in Inner Mongolia.

The unreasonable use of chemical pesticides has caused serious damage to crops and the ecological environment. The botanical pesticide matrine has attracted attention as an environmentally friendly pesticide. Compared with traditional spraying methods, unmanned aerial vehicle (UAV) spraying has the advantages of safety, rapidity, uniform droplets, low dosages, and no terrain or crop restrictions. In this study, matrine OD was prepared according to the application requirements of flight prevention preparations using three different emulsifiers. The stability, wettability, particle size and distribution, and spraying performance of matrine OD were studied. The results indicated that when the amount of emulsifier was 8%, the three types of matrine OD had good stability. The stability, wettability, particle size and distribution, and spray performance of the suspension prepared using emulsifier VO/03 were better than the other two emulsifiers. Therefore, matrine OD prepared using 8% VO/03 could be used for ultra-low-volume sprays and aerial applications. In this study, we provide a theoretical basis and technical guidance to develop pesticide formulations for aerial applications.

Delayed chilling stress is a frequent meteorological disaster in the spring maize-growing region of Northern China. Understanding the physiological responses and key characteristics of cold-tolerant maize varieties under such stress is crucial for their selection and utilization. This study compared the physiological and biochemical responses of a cold-tolerant variety (XY335) and a conventional variety (KH8) to simulated delayed chilling stress induced by early field sowing. Results showed that the emergence percentage and emergence uniformity of the cold-tolerant variety were 9.6% and 2.8% higher than those of the conventional variety, respectively. Under chilling stress, the root diameter of the cold-tolerant variety remained stable, while root length decreased by 24.5%. In contrast, the conventional variety exhibited the opposite response. Growth of the cold-tolerant variety slowed during stress but accelerated significantly after temperature recovery, achieving 1–2 more leaf ages than the conventional variety. The SPAD value (chlorophyll content) of the cold-tolerant variety was less affected, remaining 14.3% higher than the conventional variety, thereby maintaining higher photosynthetic efficiency. The enhanced stress tolerance of XY335 correlated with a robust antioxidant system: leaf peroxidase (POD) activity was 60.7% higher, and malondialdehyde (MDA) content was 42.4% lower compared to KH8. In summary, under delayed chilling stress, the cold-tolerant variety ensured higher emergence and seedling uniformity by reducing coleoptile length, maintained root diameter and absorption capacity by shortening root length, preserved chlorophyll synthesis and photosynthetic performance under the protection of a POD-dominated enzyme system, and employed a “standby mode” with compensatory leaf growth to ensure adequate dry matter accumulation and yield formation.