Chitosan films entrapped with the Mansoa hirsuta fraction (CMHF) was developed as a new dressing for wound care. The chromatographic profile of the M. hirsuta fraction (MHF) was evaluated by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry, and the results showed that MHF is rich in acid triterpenes. Physicochemical characterization of the films prepared using the solvent casting method was performed by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetry (TGA), differential scanning calorimetry (DCS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and mechanical properties. CMHF exhibited characteristic bands of both chitosan and MHF, revealing a physical mixture of both. CMHF presented an amorphous nature, thermostability, and dispersion of MHF in the chitosan matrix, resulting in a rough structure. Incorporation of M. hirsuta fraction into chitosan matrix favorably enhanced the mechanical performance and films thickness. The in vivo wound treatment with CMHF for seven days showed a characteristic area of advanced healing, re-epithelization, cell proliferation, and collagen formation. Furthermore, wound closure reached 100% contraction after 10 days of treatment with modulation of interleukins. The incorporation of M. hirsuta fraction into chitosan films was advantageous and showed great potential for stimulating wound repair and regeneration.

Mansoa hirsuta (Bignoniaceae) is a native plant from caatinga in Brazilian semiarid. This plant has been locally used as antimicrobial and hypoglycemiant agents, but their action mechanisms and toxicity remain largely unknown. Therefore, we evaluated the composition and antioxidant, cytoprotective and hypoglycemiant effects of raw extract, fractions and compounds from leaves of M. hirsuta. The cytogenotoxic effects of ursolic and oleanolic acids, the main phytotherapic components of this plant, were assessed. The raw extract and fractions presented steroids, saponins, flavonols, flavanonols, flavanones, xanthones, phenols, tannins, anthocyanins, anthocyanidins and flavonoids. The ethyl acetate fraction inhibited efficiently the cascade of lipid peroxidation while the hydroalcoholic fraction was richer in total phenols and more efficient in capturing 2,2-diphenyl-1-picrylhydrazyl (^·DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS^·+) radicals. The isolated fraction of M. hirsuta also inhibited the α-amylase activity. Cytotoxic effects were absent in both raw extract and fractions while ursolic+oleanolic acids were efficient in protecting cells after exposure to hydrogen peroxide. Moreover, this mixture of acid shad no significant interference on the mitotic index and frequency of nuclear and/or chromosomal abnormalities in Allium cepa test. Therefore, M. hirsuta represents a potential source of phytochemicals against inflammatory and oxidative pathologies, including diabetes.

Mansoa hirsuta is a medicinal plant native to the Brazilian semi-arid region. This approach aimed to investigate the in vitro and in vivo toxicity and anti-inflammatory and analgesic actions of the M. hirsuta fraction (MHF). In vitro cell viability was assessed in 3T3 cells. In vivo, the acute toxicity test, a single dose of the MHF was administered. For the subchronic toxicity test, three doses of were administered for 30 days. Locomotion and motor coordination were assessed using open field and rota-rod. The anti-inflammatory activity was evaluated in carrageenan-induced paw edema and zymosan-induced air-pouch models. Myeloperoxidase (MPO) and total proteins were also measured. The antinociceptive activity MHF was determined using acid acetic-induced abdominal writhing and formalin models. In the cytotoxicity assay, MHF showed no significative impairment of cell viability and in the acute toxicity study, did not cause mortality or signs of toxicity. Repeated exposure to MHF did not cause relevant toxicological changes. The evaluation in the open field test showed that the MHF did not alter the locomotor activity and there was no change in motor coordination and balance of animals. MHF significantly reduced edema, MPO production, the migration of leukocytes and protein leakage. In addition, MHF reduced abdominal writhing and significantly inhibited the first and second stage of the formalin test. The results of this study indicated that MHF has an anti-inflammatory and analgesic potential without causing acute or subchronic toxic effects and it can be a promising natural source to be explored.

O aumento do uso de medicamentos para emagrecimento revela a busca por resultados rápidos trazendo consigo riscos significativos. Desde inibidores de apetite e de absorção de gordura, essas opções prometem eficácia imediata, mas frequentemente causam efeitos adversos como insônia, irritabilidade, aumento da pressão arterial e náuseas. Vários desses medicamentos são proibidos em diversos países devido aos problemas de saúde que podem causar, incluindo doenças cardíacas, depressão e até mesmo risco de morte. A pesquisa se baseia em 19 estudos bibliográficos criteriosamente selecionados, dentre 61 que foram analisados. Os resultados destacam os perigos associados aos medicamentos para emagrecimento, como sibutramina, femproporex, orlistate, fentermina, fenfluramina, dexfenfluramina, agonistas do receptor de GLP-1 (liraglutida, semaglutida) e a combinação de bupropiona com naltrexona. Assim, este estudo visa abordar sobre os perigos desses medicamentos, enfatizando a importância da informação detalhada ao paciente sobre riscos, benefícios, efeitos colaterais e uso fora das indicações promovendo desta forma uma compreensão mais holística da abordagem ao emagrecimento, destacando a necessidade de orientação profissional e escolhas saudáveis.

Artrite reumatoide é uma doença crônica das articulações, cuja inflamação é provocada por alterações no sistema imunológico. O tratamento é medicamentoso e varia de acordo com o estágio da doença, sua atividade e gravidade. O uso de plantas medicinais como alternativa terapêutica tem ganhado destaque nos últimos anos, sendo a romã (Punica granatum L.) uma das plantas mais estudadas para o tratamento da doença. Assim, o objetivo do estudo é averiguar as principais evidências científicas atuais em modelo animal e em humanos sobre os benefícios da romã como alternativa terapêutica na artrite reumatoide. Foram selecionados estudos pré-clínicos e clínicos associados ao tema em questão. Os dados indicam que o extrato da romã possui atividade antioxidante e anti-inflamatória, além de melhorar os sinais clínicos-inflamatórios da artrite reumatoide. Dessa forma, o presente artigo fornece evidências sobre a eficácia da romã na AR e justifica a importância de estudos clínicos adicionais.