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Jan Matysiak
Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, 60-780 Poznań, Poland

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Review
Published: 27 July 2021 in Pharmaceuticals
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Gynecological and breast cancers still remain a significant health problem worldwide. Diagnostic methods are not sensitive and specific enough to detect the disease at an early stage. During carcinogenesis and tumor progression, the cellular need for DNA and protein synthesis increases leading to changes in the levels of amino acids. An important role of amino acids in many biological pathways, including biosynthesis of proteins, nucleic acids, enzymes, etc., which serve as an energy source and maintain redox balance, has been highlighted in many research articles. The aim of this review is a detailed analysis of the literature on metabolomic studies of gynecology and breast cancers with particular emphasis on alterations in free amino acid profiles. The work includes a brief overview of the metabolomic methodology and types of biological samples used in the studies. Special attention was paid to the possible role of selected amino acids in the carcinogenesis, especially proline and amino acids related to its metabolism. There is a clear need for further research and multiple external validation studies to establish the role of amino acid profiling in diagnosing gynecological and breast cancers.

ACS Style

Dagmara Pietkiewicz; Agnieszka Klupczynska-Gabryszak; Szymon Plewa; Magdalena Misiura; Agnieszka Horala; Wojciech Miltyk; Ewa Nowak-Markwitz; Zenon Kokot; Jan Matysiak. Free Amino Acid Alterations in Patients with Gynecological and Breast Cancer: A Review. Pharmaceuticals 2021, 14, 731 .

AMA Style

Dagmara Pietkiewicz, Agnieszka Klupczynska-Gabryszak, Szymon Plewa, Magdalena Misiura, Agnieszka Horala, Wojciech Miltyk, Ewa Nowak-Markwitz, Zenon Kokot, Jan Matysiak. Free Amino Acid Alterations in Patients with Gynecological and Breast Cancer: A Review. Pharmaceuticals. 2021; 14 (8):731.

Chicago/Turabian Style

Dagmara Pietkiewicz; Agnieszka Klupczynska-Gabryszak; Szymon Plewa; Magdalena Misiura; Agnieszka Horala; Wojciech Miltyk; Ewa Nowak-Markwitz; Zenon Kokot; Jan Matysiak. 2021. "Free Amino Acid Alterations in Patients with Gynecological and Breast Cancer: A Review." Pharmaceuticals 14, no. 8: 731.

Review
Published: 30 June 2021 in Molecules
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Bee products have been known for centuries for their versatile healing properties. In recent decades they have become the subject of documented scientific research. This review aims to present and compare the impact of bee products and their components as antimicrobial agents. Honey, propolis, royal jelly and bee venom are bee products that have antibacterial properties. Sensitivity of bacteria to these products varies considerably between products and varieties of the same product depending on their origin. According to the type of bee product, different degrees of activity were observed against Gram-positive and Gram-negative bacteria, yeasts, molds and dermatophytes, as well as biofilm-forming microorganisms. Pseudomonas aeruginosa turned out to be the most resistant to bee products. An analysis of average minimum inhibitory concentration values for bee products showed that bee venom has the strongest bacterial effectiveness, while royal jelly showed the weakest antibacterial activity. The most challenging problems associated with using bee products for medical purposes are dosage and safety. The complexity and variability in composition of these products raise the need for their standardization before safe and predictable clinical uses can be achieved.

ACS Style

Magdalena Ratajczak; Dorota Kaminska; Eliza Matuszewska; Elżbieta Hołderna-Kedzia; Jarosław Rogacki; Jan Matysiak. Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products. Molecules 2021, 26, 4007 .

AMA Style

Magdalena Ratajczak, Dorota Kaminska, Eliza Matuszewska, Elżbieta Hołderna-Kedzia, Jarosław Rogacki, Jan Matysiak. Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products. Molecules. 2021; 26 (13):4007.

Chicago/Turabian Style

Magdalena Ratajczak; Dorota Kaminska; Eliza Matuszewska; Elżbieta Hołderna-Kedzia; Jarosław Rogacki; Jan Matysiak. 2021. "Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products." Molecules 26, no. 13: 4007.

Journal article
Published: 25 June 2021 in Journal of Clinical Medicine
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Polycystic ovary syndrome (PCOS) is the most prevalent endocrine and metabolic disorder, affecting 5–10% of women of reproductive age. It results from complex environmental factors, genetic predisposition, hyperinsulinemia, hormonal imbalance, neuroendocrine abnormalities, chronic inflammation, and autoimmune disorders. PCOS impacts menstrual regularities, fertility, and dermatological complications, and may induce metabolic disturbances, diabetes, and coronary heart disease. Comprehensive metabolic profiling of patients with PCOS may be a big step in understanding and treating the disease. The study aimed to search for potential differences in metabolites concentrations among women with PCOS according to different body mass index (BMI) in comparison to healthy controls. We used broad-spectrum targeted metabolomics to evaluate metabolites’ serum concentrations in PCOS patients and compared them with healthy controls. The measurements were performed using high-performance liquid chromatography coupled with the triple quadrupole tandem mass spectrometry technique, which has highly selective multiple reaction monitoring modes. The main differences were found in glycerophospholipid concentrations, with no specific tendency to up-or down-regulation. Insulin resistance and elevated body weight influence acylcarnitine C2 levels more than PCOS itself. Sphingomyelin (SM) C18:1 should be more intensively observed and examined in future studies and maybe serve as one of the PCOS biomarkers. No significant correlations were observed between anthropometric and hormonal parameters and metabolome results.

ACS Style

Katarzyna Ożegowska; Szymon Plewa; Urszula Mantaj; Leszek Pawelczyk; Jan Matysiak. Serum Metabolomics in PCOS Women with Different Body Mass Index. Journal of Clinical Medicine 2021, 10, 2811 .

AMA Style

Katarzyna Ożegowska, Szymon Plewa, Urszula Mantaj, Leszek Pawelczyk, Jan Matysiak. Serum Metabolomics in PCOS Women with Different Body Mass Index. Journal of Clinical Medicine. 2021; 10 (13):2811.

Chicago/Turabian Style

Katarzyna Ożegowska; Szymon Plewa; Urszula Mantaj; Leszek Pawelczyk; Jan Matysiak. 2021. "Serum Metabolomics in PCOS Women with Different Body Mass Index." Journal of Clinical Medicine 10, no. 13: 2811.

Journal article
Published: 07 May 2021 in Molecules
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Royal jelly (RJ) is a complex, creamy secretion produced by the glands of worker bees. Due to its health-promoting properties, it is used by humans as a dietary supplement. However, RJ compounds are not fully characterized yet. Hence, in this research, we aimed to broaden the knowledge of the proteomic composition of fresh RJ. Water extracts of the samples were pre-treated using combinatorial hexapeptide ligand libraries (ProteoMinerTM kit), trypsin-digested, and analyzed by a nanoLC-MALDI-TOF/TOF MS system. To check the ProteoMinerTM performance in the MS-based protein identification, we also examined RJ extracts that were not prepared with the ProteoMinerTM kit. We identified a total of 86 proteins taxonomically classified to Apis spp. (bees). Among them, 74 proteins were detected in RJ extracts pre-treated with ProteoMinerTM kit, and only 50 proteins were found in extracts non-enriched with this technique. Ten of the identified features were hypothetical proteins whose existence has been predicted, but any experimental evidence proves their in vivo expression. Additionally, we detected four uncharacterized proteins of unknown functions. The results of this research indicate that the ProteoMinerTM strategy improves proteomic identification in complex biological samples. Broadening the knowledge of RJ composition may contribute to the development of standards and regulations, enhancing the quality of RJ, and consequently, the safety of its supplementation.

ACS Style

Eliza Matuszewska; Joanna Matysiak; Grzegorz Rosiński; Elżbieta Kędzia; Weronika Ząbek; Jarosław Zawadziński; Jan Matysiak. Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples. Molecules 2021, 26, 2762 .

AMA Style

Eliza Matuszewska, Joanna Matysiak, Grzegorz Rosiński, Elżbieta Kędzia, Weronika Ząbek, Jarosław Zawadziński, Jan Matysiak. Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples. Molecules. 2021; 26 (9):2762.

Chicago/Turabian Style

Eliza Matuszewska; Joanna Matysiak; Grzegorz Rosiński; Elżbieta Kędzia; Weronika Ząbek; Jarosław Zawadziński; Jan Matysiak. 2021. "Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples." Molecules 26, no. 9: 2762.

Journal article
Published: 21 April 2021 in Molecules
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Beehive products possess nutritional value and health-promoting properties and are recommended as so-called “superfoods”. However, because of their natural origin, they may contain relevant elemental contaminants. Therefore, to assess the quality of bee products, we examined concentrations of a broad range of 24 selected elements in propolis, bee pollen, and royal jelly. The quantitative analyses were performed with inductively coupled plasma-mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES) techniques. The results of our research indicate that bee products contain essential macronutrients (i.e., K, P, and S) and micronutrients (i.e., Zn and Fe) in concentrations depending on the products’ type. However, the presence of toxic heavy metals makes it necessary to test the quality of bee products before using them as dietary supplements. Bearing in mind that bee products are highly heterogenous and, depending on the environmental factors, differ in their elemental content, it is necessary to develop standards regulating the acceptable levels of inorganic pollutants. Furthermore, since bees and their products are considered to be an effective biomonitoring tool, our results may reflect the environment’s condition in west-central Poland, affecting the health and well-being of both humans and bees.

ACS Style

Eliza Matuszewska; Agnieszka Klupczynska; Krzysztof Maciołek; Zenon Kokot; Jan Matysiak. Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland. Molecules 2021, 26, 2415 .

AMA Style

Eliza Matuszewska, Agnieszka Klupczynska, Krzysztof Maciołek, Zenon Kokot, Jan Matysiak. Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland. Molecules. 2021; 26 (9):2415.

Chicago/Turabian Style

Eliza Matuszewska; Agnieszka Klupczynska; Krzysztof Maciołek; Zenon Kokot; Jan Matysiak. 2021. "Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland." Molecules 26, no. 9: 2415.

Journal article
Published: 11 April 2021 in International Journal of Environmental Research and Public Health
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Hymenoptera venom allergy significantly affects the quality of life. Due to the divergences in the results of the available test and clinical symptoms of patients, the current widely applied diagnostic methods are often insufficient to classify patients for venom immunotherapy (VIT). Therefore it is still needed to search for new, more precise, and accurate diagnostic methods. Hence, this research aimed to discover new biomarkers of Hymenoptera venom allergy in a group of inflammation factors using set of multi-marker Bioplex panel. The adoption of a novel methodology based on Luminex/xMAP enabled simultaneous determination of serum levels of 37 different inflammatory proteins in one experiment. The study involved 21 patients allergic to wasp and/or honey bee venom and 42 healthy participants. According to univariate and multivariate statistics, soluble CD30/tumor necrosis factor receptor superfamily, member 8 (sCD30/TNFRSF8), and the soluble tumor necrosis factor receptor 1 (sTNF-R1) may be considered as effective prognostic factors, their circulating levels were significantly decreased in the allergy group (p-value < 0.05; the Area Under the Curve (AUC) ~0.7; Variable Importance in Projection (VIP) scores >1.2). The obtained results shed new light on the allergic inflammatory response and may contribute to modification and improvement of the diagnostic and monitoring methods. Further, large-scale studies are still needed to explain mechanisms of action of studied compounds and to definitively prove their usefulness in clinical practice.

ACS Style

Kacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health 2021, 18, 4011 .

AMA Style

Kacper Packi, Joanna Matysiak, Eliza Matuszewska, Anna Bręborowicz, Zdzisława Kycler, Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health. 2021; 18 (8):4011.

Chicago/Turabian Style

Kacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. 2021. "New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors." International Journal of Environmental Research and Public Health 18, no. 8: 4011.

Journal article
Published: 12 March 2021 in Vaccines
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Venom immunotherapy (VIT) is administered to allergic patients to reduce the risk of dangerous systemic reactions following an insect sting. To better understand the mechanism of this treatment and its impact on the human organism, we analysed serum proteomic patterns obtained at five time-points from Hymenoptera-venom-allergic patients undergoing VIT. For statistical analyses, patients were additionally divided into two groups (high responders and low responders) according to serum sIgG4 levels. VIT was found to be associated with changes in seven proteins: the fibrinogen alpha chain, complement C4-A, complement C3, filamin-B, kininogen-1, myosin-9 and inter-alpha-trypsin inhibitor heavy chain H1. The number of discriminative m/z (mass-to-charge ratio) features increased up to the 90th day of VIT, which may be associated with the development of immunity after the administration of increased venom doses. It may also suggest that during VIT, there may occur processes involved not only in protein synthesis but also in protein degradation (caused by proteolytic venom components). The results are consistent with measured serum sIgG4 levels, which increased from 2.04 mgA/I at baseline to 7.25 mgA/I at 90 days. Moreover, the major proteomic changes were detected separately in the high responder group. This may suggest that changes in protein–peptide profiles reflect the actual response to VIT.

ACS Style

Joanna Matysiak; Eliza Matuszewska; Marek Kowalski; Sławomir Kosiński; Ewa Smorawska-Sabanty; Jan Matysiak. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns. Vaccines 2021, 9, 249 .

AMA Style

Joanna Matysiak, Eliza Matuszewska, Marek Kowalski, Sławomir Kosiński, Ewa Smorawska-Sabanty, Jan Matysiak. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns. Vaccines. 2021; 9 (3):249.

Chicago/Turabian Style

Joanna Matysiak; Eliza Matuszewska; Marek Kowalski; Sławomir Kosiński; Ewa Smorawska-Sabanty; Jan Matysiak. 2021. "Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns." Vaccines 9, no. 3: 249.

Journal article
Published: 25 February 2021 in International Journal of Environmental Research and Public Health
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Introduction: Protein profiling allows the determination of the presence of proteins marking various stages of the disease, and differentiates between people at risk of various diseases. In type 1 diabetes, protein profiling had been previously used to find blood markers other than islet autoantibodies to indicate the pancreatic beta cell destruction process and to reflect the progression of type 1 diabetes mellitus (T1DM). However, T1DM is an auto-immune disease and its clinical presentation changes in time of its duration. The aim of the study: To find differences in protein profiles in patients with type 1 diabetes according to diabetes control (HbA1c > 7%) and with presence of diabetic complications or obesity. It may help to identify subgroups of patients who may need a better clinical supervision and individualized treatment. Material and methods: A group of 103 patients with auto-immunologically confirmed T1DM, and meeting the following inclusion criteria: Caucasian race, duration of diabetes >5 years, were used in the study. Criteria of exclusion: past or present cancer (treated with chemo-/radiotherapy), diseases of the liver (ALT > 3 × ULN) except for people with simple hepatic steatosis, chronic renal disease (eGFR < 30 mL/1.73 m2/min), and acute inflammation (CRP > 5 mg/dL). The study group was divided in terms of the presence of chronic complications, obesity, or poor metabolic control (HbA1c > 7%). Protein profiling was completed by using the MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) analyzer. Results: Differentiating proteins were identified in all of the groups. The groups burdened with complications, obesity, and poor metabolic control were characterized by increased levels of fibrinogen, complement C4 and C3. Conclusion: The groups of type 1 diabetes patients burdened with complications, obesity, and poor metabolic control were characterized by increased levels of fibrinogen, complement C4 and C3. Further detailed studies are necessary to determine more subtle changes in the proteomic profile of patients with type 1 diabetes.

ACS Style

Agnieszka Zawada; Dariusz Naskręt; Eliza Matuszewska; Zenon Kokot; Marian Grzymisławski; Dorota Zozulińska-Ziółkiewicz; Agnieszka Dobrowolska; Jan Matysiak. MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications. International Journal of Environmental Research and Public Health 2021, 18, 2263 .

AMA Style

Agnieszka Zawada, Dariusz Naskręt, Eliza Matuszewska, Zenon Kokot, Marian Grzymisławski, Dorota Zozulińska-Ziółkiewicz, Agnieszka Dobrowolska, Jan Matysiak. MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications. International Journal of Environmental Research and Public Health. 2021; 18 (5):2263.

Chicago/Turabian Style

Agnieszka Zawada; Dariusz Naskręt; Eliza Matuszewska; Zenon Kokot; Marian Grzymisławski; Dorota Zozulińska-Ziółkiewicz; Agnieszka Dobrowolska; Jan Matysiak. 2021. "MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications." International Journal of Environmental Research and Public Health 18, no. 5: 2263.

Review
Published: 23 February 2021 in International Journal of Environmental Research and Public Health
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Proper preoperative ovarian cancer (OC) diagnosis remains challenging. Serum free amino acid (SFAA) profiles were investigated to identify potential novel biomarkers of OC and assess their performance in ovarian tumor differential diagnosis. Serum samples were divided based on the histopathological result: epithelial OC (n = 38), borderline ovarian tumors (n = 6), and benign ovarian tumors (BOTs) (n = 62). SFAA profiles were evaluated using aTRAQ methodology based on high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Levels of eleven amino acids significantly differed between OC+borderline and BOTs. The highest area under the receiver operating characteristic curve (AUC of ROC) (0.787) was obtained for histidine. Cystine and histidine were identified as best single markers for early stage OC/BOT and type I OC. For advanced stage OC, seven amino acids differed significantly between the groups and citrulline obtained the best AUC of 0.807. Between type II OC and BOTs, eight amino acids differed significantly and the highest AUC of 0.798 was achieved by histidine and citrulline (AUC of 0.778). Histidine was identified as a potential new biomarker in differential diagnosis of ovarian tumors. Adding histidine to a multimarker panel together with CA125 and HE4 improved the differential diagnosis between OC and BOTs.

ACS Style

Agnieszka Horala; Szymon Plewa; Pawel Derezinski; Agnieszka Klupczynska; Jan Matysiak; Ewa Nowak-Markwitz; Zenon Kokot. Serum Free Amino Acid Profiling in Differential Diagnosis of Ovarian Tumors—A Comparative Study with Review of the Literature. International Journal of Environmental Research and Public Health 2021, 18, 2167 .

AMA Style

Agnieszka Horala, Szymon Plewa, Pawel Derezinski, Agnieszka Klupczynska, Jan Matysiak, Ewa Nowak-Markwitz, Zenon Kokot. Serum Free Amino Acid Profiling in Differential Diagnosis of Ovarian Tumors—A Comparative Study with Review of the Literature. International Journal of Environmental Research and Public Health. 2021; 18 (4):2167.

Chicago/Turabian Style

Agnieszka Horala; Szymon Plewa; Pawel Derezinski; Agnieszka Klupczynska; Jan Matysiak; Ewa Nowak-Markwitz; Zenon Kokot. 2021. "Serum Free Amino Acid Profiling in Differential Diagnosis of Ovarian Tumors—A Comparative Study with Review of the Literature." International Journal of Environmental Research and Public Health 18, no. 4: 2167.

Journal article
Published: 19 February 2021 in International Journal of Molecular Sciences
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Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction.

ACS Style

Magdalena Kulus; Wiesława Kranc; Katarzyna Wojtanowicz-Markiewicz; Piotr Celichowski; Agata Światły-Błaszkiewicz; Eliza Matuszewska; Patrycja Sujka-Kordowska; Aneta Konwerska; Maciej Zdun; Rut Bryl; Maria Wieczorkiewicz; Jakub Kulus; Bogumiła Stelmach; Katarzyna Stefańska; Joanna Budna-Tukan; James Petitte; Paul Mozdziak; Kornel Ratajczak; Jan Matysiak; Jędrzej Jaśkowski; Michał Nowicki; Bartosz Kempisty. New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes. International Journal of Molecular Sciences 2021, 22, 2082 .

AMA Style

Magdalena Kulus, Wiesława Kranc, Katarzyna Wojtanowicz-Markiewicz, Piotr Celichowski, Agata Światły-Błaszkiewicz, Eliza Matuszewska, Patrycja Sujka-Kordowska, Aneta Konwerska, Maciej Zdun, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Bogumiła Stelmach, Katarzyna Stefańska, Joanna Budna-Tukan, James Petitte, Paul Mozdziak, Kornel Ratajczak, Jan Matysiak, Jędrzej Jaśkowski, Michał Nowicki, Bartosz Kempisty. New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes. International Journal of Molecular Sciences. 2021; 22 (4):2082.

Chicago/Turabian Style

Magdalena Kulus; Wiesława Kranc; Katarzyna Wojtanowicz-Markiewicz; Piotr Celichowski; Agata Światły-Błaszkiewicz; Eliza Matuszewska; Patrycja Sujka-Kordowska; Aneta Konwerska; Maciej Zdun; Rut Bryl; Maria Wieczorkiewicz; Jakub Kulus; Bogumiła Stelmach; Katarzyna Stefańska; Joanna Budna-Tukan; James Petitte; Paul Mozdziak; Kornel Ratajczak; Jan Matysiak; Jędrzej Jaśkowski; Michał Nowicki; Bartosz Kempisty. 2021. "New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes." International Journal of Molecular Sciences 22, no. 4: 2082.

Journal article
Published: 18 October 2020 in International Journal of Environmental Research and Public Health
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This study presents the use of matrix-assisted laser desorption and ionization mass spectrometry imaging (MALDI-MSI) directly on the tissue of two ovarian tumors that often present a diagnostic challenge, a low-grade serous borderline ovarian tumor and ovarian fibrothecoma. Different spatial distribution of m/z values within the tissue samples was observed, and regiospecific peaks were identified. Among the 106 peaks in the borderline ovarian tumor five, regiospecific peaks (m/z: 2861.35; 2775.79; 3368.34; 3438.43; 4936.37) were selected using FlexImaging software. Subsequently, the distribution of those selected peaks was visualized on the fibrothecoma tissue section, which demonstrated the differences in the tissue homo-/heterogeneous structure of both tumors. The comparison with the histopathological staining of the ovarian borderline tumor tissue section, obtained during serial sectioning, showed a close correlation of the molecular map with the morphological and histopathological features of the tissue and allowed the identification of different tissue types within the sample. This study highlights the potential significance of MSI in enabling morphological characterization of ovarian tumors as well as correct diagnosis and further prognosis than thus far seen in the literature. Osteopontin, tropomyosin and orosomucoid are only a couple of the molecules investigated using MALDI-MSI in ovarian cancer research. This study, in line with the available literature, proves the potential of MALDI-MSI to overcome the current limitations of classic histopathological examination giving a more in-depth insight into the tissue structure and thus lead to the more accurate differential diagnosis of ovarian tumors, especially in the most challenging cases.

ACS Style

Dagmara Pietkiewicz; Agnieszka Horała; Szymon Plewa; Piotr Jasiński; Ewa Nowak-Markwitz; Zenon J. Kokot; Jan Matysiak. MALDI-MSI—A Step Forward in Overcoming the Diagnostic Challenges in Ovarian Tumors. International Journal of Environmental Research and Public Health 2020, 17, 7564 .

AMA Style

Dagmara Pietkiewicz, Agnieszka Horała, Szymon Plewa, Piotr Jasiński, Ewa Nowak-Markwitz, Zenon J. Kokot, Jan Matysiak. MALDI-MSI—A Step Forward in Overcoming the Diagnostic Challenges in Ovarian Tumors. International Journal of Environmental Research and Public Health. 2020; 17 (20):7564.

Chicago/Turabian Style

Dagmara Pietkiewicz; Agnieszka Horała; Szymon Plewa; Piotr Jasiński; Ewa Nowak-Markwitz; Zenon J. Kokot; Jan Matysiak. 2020. "MALDI-MSI—A Step Forward in Overcoming the Diagnostic Challenges in Ovarian Tumors." International Journal of Environmental Research and Public Health 17, no. 20: 7564.

Journal article
Published: 12 October 2020 in Molecules
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A growing interest in metabolomics studies of cultured cells requires development not only untargeted methods capable of fingerprinting the complete metabolite profile but also targeted methods enabling the precise and accurate determination of a selected group of metabolites. Proline metabolism affects many crucial processes at the cellular level, including collagen biosynthesis, redox balance, energetic processes as well as intracellular signaling. The study aimed to develop a robust and easy-to-use targeted metabolomics method for the determination of the intracellular level of proline and the other two amino acids closely related to proline metabolism: glutamic acid and arginine. The method employs hydrophilic interaction liquid chromatography followed by high-resolution, accurate-mass mass spectrometry for reliable detection and quantification of the target metabolites in cell lysates. The sample preparation consisted of quenching by the addition of ice-cold methanol and subsequent cell scraping into a quenching solution. The method validation showed acceptable linearity (r > 0.995), precision (%RSD < 15%), and accuracy (88.5–108.5%). Pilot research using HaCaT spontaneously immortalized human keratinocytes in a model for wound healing was performed, indicating the usefulness of the method in studies of disturbances in proline metabolism. The developed method addresses the need to determine the intracellular concentration of three key amino acids and can be used routinely in targeted mammalian cell culture metabolomics research.

ACS Style

Agnieszka Klupczynska; Magdalena Misiura; Wojciech Miltyk; Ilona Oscilowska; Jerzy Palka; Zenon J. Kokot; Jan Matysiak. Development of an LC-MS Targeted Metabolomics Methodology to Study Proline Metabolism in Mammalian Cell Cultures. Molecules 2020, 25, 4639 .

AMA Style

Agnieszka Klupczynska, Magdalena Misiura, Wojciech Miltyk, Ilona Oscilowska, Jerzy Palka, Zenon J. Kokot, Jan Matysiak. Development of an LC-MS Targeted Metabolomics Methodology to Study Proline Metabolism in Mammalian Cell Cultures. Molecules. 2020; 25 (20):4639.

Chicago/Turabian Style

Agnieszka Klupczynska; Magdalena Misiura; Wojciech Miltyk; Ilona Oscilowska; Jerzy Palka; Zenon J. Kokot; Jan Matysiak. 2020. "Development of an LC-MS Targeted Metabolomics Methodology to Study Proline Metabolism in Mammalian Cell Cultures." Molecules 25, no. 20: 4639.

Review
Published: 18 September 2020 in International Journal of Environmental Research and Public Health
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Dietary supplements cover a wide range of products, the most popular are those containing plant-based ingredients. Supplements are consumed by consumers of all ages as well as by both healthy and sick people. The lack of unified regulation in this sector increases the probability that supplements are poor chemical and microbiological quality and can be dangerous for patients. The aim of this paper is to highlight selected issues associated with the microbiological quality of dietary supplements containing plant materials. We focus on the most recent reports referring to bacterial and fungal contaminations as well as the presence of mycotoxins. Dietary supplements containing plant ingredients commonly show a variety of microbial contaminants, which might be crucial for consumer safety. They often contain microorganisms potentially pathogenic to humans. Metabolites produced by microorganisms may pose a threat to the health of consumers. Because of that, in this review, we emphasize the risk that may be associated with the lack of appropriate studies of the quality of the supplements.

ACS Style

Magdalena Ratajczak; Dorota Kaminska; Agata Światły-Błaszkiewicz; Jan Matysiak. Quality of Dietary Supplements Containing Plant-Derived Ingredients Reconsidered by Microbiological Approach. International Journal of Environmental Research and Public Health 2020, 17, 6837 .

AMA Style

Magdalena Ratajczak, Dorota Kaminska, Agata Światły-Błaszkiewicz, Jan Matysiak. Quality of Dietary Supplements Containing Plant-Derived Ingredients Reconsidered by Microbiological Approach. International Journal of Environmental Research and Public Health. 2020; 17 (18):6837.

Chicago/Turabian Style

Magdalena Ratajczak; Dorota Kaminska; Agata Światły-Błaszkiewicz; Jan Matysiak. 2020. "Quality of Dietary Supplements Containing Plant-Derived Ingredients Reconsidered by Microbiological Approach." International Journal of Environmental Research and Public Health 17, no. 18: 6837.

Coloss beebook
Published: 01 September 2020 in Journal of Apicultural Research
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Honey bees have a sting which allows them to inject venomous substances into the body of an opponent or attacker. As the sting originates from a modified ovipositor, it only occurs in the female insect, and this is a defining feature of the bee species that belong to a subclade of the Hymenoptera called Aculeata. There is considerable interest in bee venom research, primarily because of an important subset of the human population who will develop a sometimes life threatening allergic response after a bee sting. However, the use of honey bee venom goes much further, with alleged healing properties in ancient therapies and recent research. The present paper aims to standardize selected methods for honey bee venom research. It covers different methods of venom collection, characterization and storage. Much attention was also addressed to the determination of the biological activity of the venom and its use in the context of biomedical research, more specifically venom allergy. Finally, the procedure for the assignment of new venom allergens has been presented. Las abejas melíferas tienen un aguijón que les permite inyectar sustancias venenosas en el cuerpo de un oponente o atacante. El aguijón es un ovipositor modificado que solo se manifiesta en el insecto hembra, siendo este una característica que define a las especies de abejas que pertenecen al subclado de himenópteros llamada Aculeata. Hay un interés considerable en la investigación del veneno de abeja, principalmente debido a que un porcentaje importante de la población humana desarrollará una respuesta alérgica - a veces mortal - a la picadura de abeja. Sin embargo, el uso del veneno de la abeja melífera abarca mucho más, con presuntas propiedades curativas en terapias antiguas e investigaciones recientes. El presente trabajo tiene como objetivo estandarizar métodos seleccionados para la investigación del veneno de las abejas melíferas. Cubre diferentes métodos de recolección, caracterización y almacenamiento de veneno. También se prestó mucha atención a la determinación de la actividad biológica del veneno y su uso en el contexto de la investigación biomédica, más específicamente la alergia al veneno. Finalmente, se ha presentado el procedimiento para la asignación de nuevos alérgenos de veneno.

ACS Style

Dirk C. De Graaf; Márcia Regina Brochetto Braga; Rusleyd Maria Magalhães De Abreu; Simon Blank; Chris H. Bridts; Luc S. De Clerck; Bart Devreese; Didier G. Ebo; Timothy J. Ferris; Margo M. Hagendorens; Débora Laís Justo Jacomini; Iliya Kanchev; Zenon J. Kokot; Jan Matysiak; Christel Mertens; Vito Sabato; Athina L. Van Gasse; Matthias Van Vaerenbergh. Standard methods for Apis mellifera venom research. Journal of Apicultural Research 2020, 1 -31.

AMA Style

Dirk C. De Graaf, Márcia Regina Brochetto Braga, Rusleyd Maria Magalhães De Abreu, Simon Blank, Chris H. Bridts, Luc S. De Clerck, Bart Devreese, Didier G. Ebo, Timothy J. Ferris, Margo M. Hagendorens, Débora Laís Justo Jacomini, Iliya Kanchev, Zenon J. Kokot, Jan Matysiak, Christel Mertens, Vito Sabato, Athina L. Van Gasse, Matthias Van Vaerenbergh. Standard methods for Apis mellifera venom research. Journal of Apicultural Research. 2020; ():1-31.

Chicago/Turabian Style

Dirk C. De Graaf; Márcia Regina Brochetto Braga; Rusleyd Maria Magalhães De Abreu; Simon Blank; Chris H. Bridts; Luc S. De Clerck; Bart Devreese; Didier G. Ebo; Timothy J. Ferris; Margo M. Hagendorens; Débora Laís Justo Jacomini; Iliya Kanchev; Zenon J. Kokot; Jan Matysiak; Christel Mertens; Vito Sabato; Athina L. Van Gasse; Matthias Van Vaerenbergh. 2020. "Standard methods for Apis mellifera venom research." Journal of Apicultural Research , no. : 1-31.

Journal article
Published: 02 July 2020 in International Journal of Environmental Research and Public Health
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Asthma often begins in childhood, although making an early diagnosis is difficult. Clinical manifestations, the exclusion of other causes of bronchial obstruction, and responsiveness to anti-inflammatory therapy are the main tool of diagnosis. However, novel, precise, and functional biochemical markers are needed in the differentiation of asthma phenotypes, endotypes, and creating personalized therapy. The aim of the study was to search for metabolomic-based asthma biomarkers among free amino acids (AAs). A wide panel of serum-free AAs in asthmatic children, covering both proteinogenic and non-proteinogenic AAs, were analyzed. The examination included two groups of individuals between 3 and 18 years old: asthmatic children and the control group consisted of children with neither asthma nor allergies. High-performance liquid chromatography combined with tandem mass spectrometry (LC-MS/MS technique) was used for AA measurements. The data were analyzed by applying uni- and multivariate statistical tests. The obtained results indicate the decreased serum concentration of taurine, L-valine, DL-β-aminoisobutyric acid, and increased levels of ƴ-amino-n-butyric acid and L-arginine in asthmatic children when compared to controls. The altered concentration of these AAs can testify to their role in the pathogenesis of childhood asthma. The authors’ results should contribute to the future introduction of new diagnostic markers into clinical practice.

ACS Style

Joanna Matysiak; Agnieszka Klupczynska; Kacper Packi; Anna Mackowiak-Jakubowska; Anna Bręborowicz; Olga Pawlicka; Katarzyna Olejniczak; Zenon J. Kokot; Jan Matysiak. Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma. International Journal of Environmental Research and Public Health 2020, 17, 4758 .

AMA Style

Joanna Matysiak, Agnieszka Klupczynska, Kacper Packi, Anna Mackowiak-Jakubowska, Anna Bręborowicz, Olga Pawlicka, Katarzyna Olejniczak, Zenon J. Kokot, Jan Matysiak. Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma. International Journal of Environmental Research and Public Health. 2020; 17 (13):4758.

Chicago/Turabian Style

Joanna Matysiak; Agnieszka Klupczynska; Kacper Packi; Anna Mackowiak-Jakubowska; Anna Bręborowicz; Olga Pawlicka; Katarzyna Olejniczak; Zenon J. Kokot; Jan Matysiak. 2020. "Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma." International Journal of Environmental Research and Public Health 17, no. 13: 4758.

Journal article
Published: 13 May 2020 in Metabolites
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Red blood cells (RBCs) are the most abundant cells in the human blood that have been extensively studied under morphology, ultrastructure, biochemical and molecular functions. Therefore, RBCs are excellent cell models in the study of biologically active compounds like drugs and toxins on the structure and function of the cell membrane. The aim of the present study was to explore erythrocyte ghost’s proteome to identify changes occurring under the influence of three bee venom peptides-melittin, tertiapin, and apamin. We conducted preliminary experiments on the erythrocyte ghosts incubated with these peptides at their non-hemolytic concentrations. Such preparations were analyzed using liquid chromatography coupled with tandem mass spectrometry. It was found that when higher concentrations of melittin and apamin were used, fewer proteins were identified. Moreover, the results clearly indicated that apamin demonstrates the greatest influence on the RBCs ghosts proteome. Interestingly, the data also suggest that tertiapin exerted a stabilizing effect on the erythrocyte membrane. The experiments carried out show the great potential of proteomic research in the projects focused on the toxin’s properties as membrane active agents. However, to determine the specificity of the effect of selected bee venom peptides on the erythrocyte ghosts, further proteomic research should be focused on the quantitative analysis.

ACS Style

Agata Światły-Błaszkiewicz; Lucyna Mrówczyńska; Eliza Matuszewska; Jan Lubawy; Arkadiusz Urbański; Zenon J. Kokot; Grzegorz Rosiński; Jan Matysiak. The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study. Metabolites 2020, 10, 191 .

AMA Style

Agata Światły-Błaszkiewicz, Lucyna Mrówczyńska, Eliza Matuszewska, Jan Lubawy, Arkadiusz Urbański, Zenon J. Kokot, Grzegorz Rosiński, Jan Matysiak. The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study. Metabolites. 2020; 10 (5):191.

Chicago/Turabian Style

Agata Światły-Błaszkiewicz; Lucyna Mrówczyńska; Eliza Matuszewska; Jan Lubawy; Arkadiusz Urbański; Zenon J. Kokot; Grzegorz Rosiński; Jan Matysiak. 2020. "The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study." Metabolites 10, no. 5: 191.

Journal article
Published: 28 December 2019 in Toxins
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Organic acids are important active small molecules present in venoms and toxins, which have not been fully explored yet. The aim of the study was the determination of organic acids in honeybee venom (HBV) samples by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two protocols for sample preparation were employed. A solid-phase extraction was used for the determination of malonic acid, fumaric acid, glutaric acid, and kynurenic acid. A dilute-and-shoot method was optimal for: citric acid, malic acid, and succinic acid. Chromatographic separation was performed using a Synergi Hydro-RP column. Detection was performed on a triple-quadrupole mass spectrometer operating in multiple reaction monitoring mode. Among the analytes, glutaric acid and kynurenic acid were present in HBV samples in the lowest concentrations, whereas citric acid was the most abundant acid in each sample, and accounted for an average of 86 mg/g (8.6%) of the venom dry weight. Organic acids were discussed in terms of function. This is the first study in the available literature that provides specific data on the content of organic acids in HBV using a validated quantitative method.

ACS Style

Magdalena Pawlak; Agnieszka Klupczynska; Zenon J Kokot; Jan Matysiak. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins 2019, 12, 14 .

AMA Style

Magdalena Pawlak, Agnieszka Klupczynska, Zenon J Kokot, Jan Matysiak. Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids. Toxins. 2019; 12 (1):14.

Chicago/Turabian Style

Magdalena Pawlak; Agnieszka Klupczynska; Zenon J Kokot; Jan Matysiak. 2019. "Extending Metabolomic Studies of Apis mellifera Venom: LC-MS-Based Targeted Analysis of Organic Acids." Toxins 12, no. 1: 14.

Journal article
Published: 27 November 2019 in Allergy, Asthma & Clinical Immunology
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Background Hymenoptera venom allergy is one of the most frequent causes of anaphylaxis. In its most severe form, the reaction to wasp and honey bee stings may be life-threatening. Therefore, immediate and proper diagnosis of venom allergy and implementation of suitable therapy are extremely important. Broadening the knowledge on the mechanism of the allergic reaction may contribute to the improvement of both diagnostic and treatment methods. Thus, this study aimed to discover changes in protein expression in serum of patients allergic to Hymenoptera (wasp and honeybee) venom and to point out proteins and peptides involved in the allergic inflammation. Methods Serum proteomic patterns typical to allergic patients and healthy volunteers were obtained with MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) mass spectrometer. The spectra were processed, analyzed and compared using advanced bioinformatics tools. The discriminative peaks were subjected to identification with liquid chromatography coupled with tandem mass spectrometry. Results This methodology allowed for the identification of four features differentiating between allergy and control groups. They were: fibrinogen alpha chain, coagulation factor XIII chain A, complement C4-A, and inter-alpha-trypsin inhibitor heavy chain H4. All of these proteins are involved in allergic inflammatory response. Conclusions Extending the knowledge of the Hymenoptera venom sensitization will contribute to the development of novel, sensitive and specific methods for quick and unambiguous allergy diagnosis. Understanding the basis of the allergy at the proteomic level will support the improvement of preventive and therapeutic measures.

ACS Style

Eliza Matuszewska; Joanna Matysiak; Anna Bręborowicz; Katarzyna Olejniczak; Zdzisława Kycler; Zenon Kokot; Jan Matysiak. Proteomic features characterization of Hymenoptera venom allergy. Allergy, Asthma & Clinical Immunology 2019, 15, 1 -8.

AMA Style

Eliza Matuszewska, Joanna Matysiak, Anna Bręborowicz, Katarzyna Olejniczak, Zdzisława Kycler, Zenon Kokot, Jan Matysiak. Proteomic features characterization of Hymenoptera venom allergy. Allergy, Asthma & Clinical Immunology. 2019; 15 (1):1-8.

Chicago/Turabian Style

Eliza Matuszewska; Joanna Matysiak; Anna Bręborowicz; Katarzyna Olejniczak; Zdzisława Kycler; Zenon Kokot; Jan Matysiak. 2019. "Proteomic features characterization of Hymenoptera venom allergy." Allergy, Asthma & Clinical Immunology 15, no. 1: 1-8.

Communication
Published: 27 August 2019 in Toxins
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Melittin (MEL) is a basic polypeptide originally purified from honeybee venom. MEL exhibits a broad spectrum of biological activity. However, almost all studies on MEL activity have been carried out on vertebrate models or cell lines. Recently, due to cheap breeding and the possibility of extrapolating the results of the research to vertebrates, insects have been used for various bioassays and comparative physiological studies. For these reasons, it is valuable to examine the influence of melittin on insect physiology. Here, for the first time, we report the immunotropic and cardiotropic effects of melittin on the beetle Tenebrio molitor as a model insect. After melittin injection at 10−7 M and 10−3 M, the number of apoptotic cells in the haemolymph increased in a dose-dependent manner. The pro-apoptotic action of MEL was likely compensated by increasing the total number of haemocytes. However, the injection of MEL did not cause any changes in the percent of phagocytic haemocytes or in the phenoloxidase activity. In an in vitro bioassay with a semi-isolated Tenebrio heart, MEL induced a slight chronotropic-positive effect only at a higher concentration (10−4 M). Preliminary results indicated that melittin exerts pleiotropic effects on the functioning of the immune system and the endogenous contractile activity of the heart. Some of the induced responses in T. molitor resemble the reactions observed in vertebrate models. Therefore, the T. molitor beetle may be a convenient invertebrate model organism for comparative physiological studies and for the identification of new properties and mechanisms of action of melittin and related compounds.

ACS Style

Jan Lubawy; Arkadiusz Urbański; Lucyna Mrówczyńska; Eliza Matuszewska; Agata Światły-Błaszkiewicz; Jan Matysiak; Grzegorz Rosiński. The Influence of Bee Venom Melittin on the Functioning of the Immune System and the Contractile Activity of the Insect Heart—A Preliminary Study. Toxins 2019, 11, 494 .

AMA Style

Jan Lubawy, Arkadiusz Urbański, Lucyna Mrówczyńska, Eliza Matuszewska, Agata Światły-Błaszkiewicz, Jan Matysiak, Grzegorz Rosiński. The Influence of Bee Venom Melittin on the Functioning of the Immune System and the Contractile Activity of the Insect Heart—A Preliminary Study. Toxins. 2019; 11 (9):494.

Chicago/Turabian Style

Jan Lubawy; Arkadiusz Urbański; Lucyna Mrówczyńska; Eliza Matuszewska; Agata Światły-Błaszkiewicz; Jan Matysiak; Grzegorz Rosiński. 2019. "The Influence of Bee Venom Melittin on the Functioning of the Immune System and the Contractile Activity of the Insect Heart—A Preliminary Study." Toxins 11, no. 9: 494.

Journal article
Published: 10 July 2019 in International Journal of Molecular Sciences
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Oviductal epithelial cells (OECs) actively produce stimulating and protecting factors, favoring survival and viability of gametes and early embryos. The oviduct participates in the initial reproductive events, which strongly depends on adhesion. The analysis of differential gene expression in OECs, during long-term in vitro culture, enables recognition of new molecular markers regulating several processes, including "biological adhesion". Porcine oviducts were stained with hematoxylin and eosin, as well as with antibodies against epithelial markers. Then, OECs were long-term in vitro cultured and after 24 h, 7, 15, and 30 days of culture were subjected to transcriptomic and proteomic assays. Microarrays were employed to evaluate gene expression, with Matrix-assisted laser desorption/ionization-time of light (MALDI-TOF) mass spectrometry applied to determine the proteome. The results revealed proper morphology of the oviducts and typical epithelial structure of OECs during the culture. From the set of differentially expressed genes (DEGs), we have selected the 130 that encoded proteins detected by MALDI-TOF MS analysis. From this gene pool, 18 significantly enriched gene ontology biological processes (GO BP) terms were extracted. Among them we focused on genes belonging to "biological adhesion" GO BP. It is suggested that increased expression of studied genes can be attributed to the process of intensive secretion of substances that exhibit favorable influence on oviductal environment, which prime gametes adhesion and viability, fertilization, and early embryo journey.

ACS Style

Joanna Budna-Tukan; Agata Światły-Błaszkiewicz; Piotr Celichowski; Sandra Kałużna; Aneta Konwerska; Patrycja Sujka-Kordowska; Maurycy Jankowski; Magdalena Kulus; Michal Jeseta; Hanna Piotrowska-Kempisty; Małgorzata Józkowiak; Paweł Antosik; Dorota Bukowska; Mariusz T. Skowroński; Jan Matysiak; Michał Nowicki; Bartosz Kempisty. "Biological Adhesion" is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study. International Journal of Molecular Sciences 2019, 20, 3387 .

AMA Style

Joanna Budna-Tukan, Agata Światły-Błaszkiewicz, Piotr Celichowski, Sandra Kałużna, Aneta Konwerska, Patrycja Sujka-Kordowska, Maurycy Jankowski, Magdalena Kulus, Michal Jeseta, Hanna Piotrowska-Kempisty, Małgorzata Józkowiak, Paweł Antosik, Dorota Bukowska, Mariusz T. Skowroński, Jan Matysiak, Michał Nowicki, Bartosz Kempisty. "Biological Adhesion" is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study. International Journal of Molecular Sciences. 2019; 20 (14):3387.

Chicago/Turabian Style

Joanna Budna-Tukan; Agata Światły-Błaszkiewicz; Piotr Celichowski; Sandra Kałużna; Aneta Konwerska; Patrycja Sujka-Kordowska; Maurycy Jankowski; Magdalena Kulus; Michal Jeseta; Hanna Piotrowska-Kempisty; Małgorzata Józkowiak; Paweł Antosik; Dorota Bukowska; Mariusz T. Skowroński; Jan Matysiak; Michał Nowicki; Bartosz Kempisty. 2019. ""Biological Adhesion" is a Significantly Regulated Molecular Process during Long-Term Primary In Vitro Culture of Oviductal Epithelial Cells (Oecs): A Transcriptomic and Proteomic Study." International Journal of Molecular Sciences 20, no. 14: 3387.