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Eliza Matuszewska
Department of Inorganic and Analytical Chemistry, Poznan University of Medical Sciences, Grunwaldzka 6, 60-780 Poznan, Poland

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Review
Published: 30 June 2021 in Molecules
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Bee products have been known for centuries for their versatile healing properties. In recent decades they have become the subject of documented scientific research. This review aims to present and compare the impact of bee products and their components as antimicrobial agents. Honey, propolis, royal jelly and bee venom are bee products that have antibacterial properties. Sensitivity of bacteria to these products varies considerably between products and varieties of the same product depending on their origin. According to the type of bee product, different degrees of activity were observed against Gram-positive and Gram-negative bacteria, yeasts, molds and dermatophytes, as well as biofilm-forming microorganisms. Pseudomonas aeruginosa turned out to be the most resistant to bee products. An analysis of average minimum inhibitory concentration values for bee products showed that bee venom has the strongest bacterial effectiveness, while royal jelly showed the weakest antibacterial activity. The most challenging problems associated with using bee products for medical purposes are dosage and safety. The complexity and variability in composition of these products raise the need for their standardization before safe and predictable clinical uses can be achieved.

ACS Style

Magdalena Ratajczak; Dorota Kaminska; Eliza Matuszewska; Elżbieta Hołderna-Kedzia; Jarosław Rogacki; Jan Matysiak. Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products. Molecules 2021, 26, 4007 .

AMA Style

Magdalena Ratajczak, Dorota Kaminska, Eliza Matuszewska, Elżbieta Hołderna-Kedzia, Jarosław Rogacki, Jan Matysiak. Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products. Molecules. 2021; 26 (13):4007.

Chicago/Turabian Style

Magdalena Ratajczak; Dorota Kaminska; Eliza Matuszewska; Elżbieta Hołderna-Kedzia; Jarosław Rogacki; Jan Matysiak. 2021. "Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products." Molecules 26, no. 13: 4007.

Journal article
Published: 07 May 2021 in Molecules
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Royal jelly (RJ) is a complex, creamy secretion produced by the glands of worker bees. Due to its health-promoting properties, it is used by humans as a dietary supplement. However, RJ compounds are not fully characterized yet. Hence, in this research, we aimed to broaden the knowledge of the proteomic composition of fresh RJ. Water extracts of the samples were pre-treated using combinatorial hexapeptide ligand libraries (ProteoMinerTM kit), trypsin-digested, and analyzed by a nanoLC-MALDI-TOF/TOF MS system. To check the ProteoMinerTM performance in the MS-based protein identification, we also examined RJ extracts that were not prepared with the ProteoMinerTM kit. We identified a total of 86 proteins taxonomically classified to Apis spp. (bees). Among them, 74 proteins were detected in RJ extracts pre-treated with ProteoMinerTM kit, and only 50 proteins were found in extracts non-enriched with this technique. Ten of the identified features were hypothetical proteins whose existence has been predicted, but any experimental evidence proves their in vivo expression. Additionally, we detected four uncharacterized proteins of unknown functions. The results of this research indicate that the ProteoMinerTM strategy improves proteomic identification in complex biological samples. Broadening the knowledge of RJ composition may contribute to the development of standards and regulations, enhancing the quality of RJ, and consequently, the safety of its supplementation.

ACS Style

Eliza Matuszewska; Joanna Matysiak; Grzegorz Rosiński; Elżbieta Kędzia; Weronika Ząbek; Jarosław Zawadziński; Jan Matysiak. Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples. Molecules 2021, 26, 2762 .

AMA Style

Eliza Matuszewska, Joanna Matysiak, Grzegorz Rosiński, Elżbieta Kędzia, Weronika Ząbek, Jarosław Zawadziński, Jan Matysiak. Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples. Molecules. 2021; 26 (9):2762.

Chicago/Turabian Style

Eliza Matuszewska; Joanna Matysiak; Grzegorz Rosiński; Elżbieta Kędzia; Weronika Ząbek; Jarosław Zawadziński; Jan Matysiak. 2021. "Mining the Royal Jelly Proteins: Combinatorial Hexapeptide Ligand Library Significantly Improves the MS-Based Proteomic Identification in Complex Biological Samples." Molecules 26, no. 9: 2762.

Journal article
Published: 21 April 2021 in Molecules
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Beehive products possess nutritional value and health-promoting properties and are recommended as so-called “superfoods”. However, because of their natural origin, they may contain relevant elemental contaminants. Therefore, to assess the quality of bee products, we examined concentrations of a broad range of 24 selected elements in propolis, bee pollen, and royal jelly. The quantitative analyses were performed with inductively coupled plasma-mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES) techniques. The results of our research indicate that bee products contain essential macronutrients (i.e., K, P, and S) and micronutrients (i.e., Zn and Fe) in concentrations depending on the products’ type. However, the presence of toxic heavy metals makes it necessary to test the quality of bee products before using them as dietary supplements. Bearing in mind that bee products are highly heterogenous and, depending on the environmental factors, differ in their elemental content, it is necessary to develop standards regulating the acceptable levels of inorganic pollutants. Furthermore, since bees and their products are considered to be an effective biomonitoring tool, our results may reflect the environment’s condition in west-central Poland, affecting the health and well-being of both humans and bees.

ACS Style

Eliza Matuszewska; Agnieszka Klupczynska; Krzysztof Maciołek; Zenon Kokot; Jan Matysiak. Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland. Molecules 2021, 26, 2415 .

AMA Style

Eliza Matuszewska, Agnieszka Klupczynska, Krzysztof Maciołek, Zenon Kokot, Jan Matysiak. Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland. Molecules. 2021; 26 (9):2415.

Chicago/Turabian Style

Eliza Matuszewska; Agnieszka Klupczynska; Krzysztof Maciołek; Zenon Kokot; Jan Matysiak. 2021. "Multielemental Analysis of Bee Pollen, Propolis, and Royal Jelly Collected in West-Central Poland." Molecules 26, no. 9: 2415.

Journal article
Published: 11 April 2021 in International Journal of Environmental Research and Public Health
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Hymenoptera venom allergy significantly affects the quality of life. Due to the divergences in the results of the available test and clinical symptoms of patients, the current widely applied diagnostic methods are often insufficient to classify patients for venom immunotherapy (VIT). Therefore it is still needed to search for new, more precise, and accurate diagnostic methods. Hence, this research aimed to discover new biomarkers of Hymenoptera venom allergy in a group of inflammation factors using set of multi-marker Bioplex panel. The adoption of a novel methodology based on Luminex/xMAP enabled simultaneous determination of serum levels of 37 different inflammatory proteins in one experiment. The study involved 21 patients allergic to wasp and/or honey bee venom and 42 healthy participants. According to univariate and multivariate statistics, soluble CD30/tumor necrosis factor receptor superfamily, member 8 (sCD30/TNFRSF8), and the soluble tumor necrosis factor receptor 1 (sTNF-R1) may be considered as effective prognostic factors, their circulating levels were significantly decreased in the allergy group (p-value < 0.05; the Area Under the Curve (AUC) ~0.7; Variable Importance in Projection (VIP) scores >1.2). The obtained results shed new light on the allergic inflammatory response and may contribute to modification and improvement of the diagnostic and monitoring methods. Further, large-scale studies are still needed to explain mechanisms of action of studied compounds and to definitively prove their usefulness in clinical practice.

ACS Style

Kacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health 2021, 18, 4011 .

AMA Style

Kacper Packi, Joanna Matysiak, Eliza Matuszewska, Anna Bręborowicz, Zdzisława Kycler, Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health. 2021; 18 (8):4011.

Chicago/Turabian Style

Kacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. 2021. "New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors." International Journal of Environmental Research and Public Health 18, no. 8: 4011.

Journal article
Published: 12 March 2021 in Vaccines
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Venom immunotherapy (VIT) is administered to allergic patients to reduce the risk of dangerous systemic reactions following an insect sting. To better understand the mechanism of this treatment and its impact on the human organism, we analysed serum proteomic patterns obtained at five time-points from Hymenoptera-venom-allergic patients undergoing VIT. For statistical analyses, patients were additionally divided into two groups (high responders and low responders) according to serum sIgG4 levels. VIT was found to be associated with changes in seven proteins: the fibrinogen alpha chain, complement C4-A, complement C3, filamin-B, kininogen-1, myosin-9 and inter-alpha-trypsin inhibitor heavy chain H1. The number of discriminative m/z (mass-to-charge ratio) features increased up to the 90th day of VIT, which may be associated with the development of immunity after the administration of increased venom doses. It may also suggest that during VIT, there may occur processes involved not only in protein synthesis but also in protein degradation (caused by proteolytic venom components). The results are consistent with measured serum sIgG4 levels, which increased from 2.04 mgA/I at baseline to 7.25 mgA/I at 90 days. Moreover, the major proteomic changes were detected separately in the high responder group. This may suggest that changes in protein–peptide profiles reflect the actual response to VIT.

ACS Style

Joanna Matysiak; Eliza Matuszewska; Marek Kowalski; Sławomir Kosiński; Ewa Smorawska-Sabanty; Jan Matysiak. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns. Vaccines 2021, 9, 249 .

AMA Style

Joanna Matysiak, Eliza Matuszewska, Marek Kowalski, Sławomir Kosiński, Ewa Smorawska-Sabanty, Jan Matysiak. Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns. Vaccines. 2021; 9 (3):249.

Chicago/Turabian Style

Joanna Matysiak; Eliza Matuszewska; Marek Kowalski; Sławomir Kosiński; Ewa Smorawska-Sabanty; Jan Matysiak. 2021. "Association between Venom Immunotherapy and Changes in Serum Protein—Peptide Patterns." Vaccines 9, no. 3: 249.

Journal article
Published: 25 February 2021 in International Journal of Environmental Research and Public Health
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Introduction: Protein profiling allows the determination of the presence of proteins marking various stages of the disease, and differentiates between people at risk of various diseases. In type 1 diabetes, protein profiling had been previously used to find blood markers other than islet autoantibodies to indicate the pancreatic beta cell destruction process and to reflect the progression of type 1 diabetes mellitus (T1DM). However, T1DM is an auto-immune disease and its clinical presentation changes in time of its duration. The aim of the study: To find differences in protein profiles in patients with type 1 diabetes according to diabetes control (HbA1c > 7%) and with presence of diabetic complications or obesity. It may help to identify subgroups of patients who may need a better clinical supervision and individualized treatment. Material and methods: A group of 103 patients with auto-immunologically confirmed T1DM, and meeting the following inclusion criteria: Caucasian race, duration of diabetes >5 years, were used in the study. Criteria of exclusion: past or present cancer (treated with chemo-/radiotherapy), diseases of the liver (ALT > 3 × ULN) except for people with simple hepatic steatosis, chronic renal disease (eGFR < 30 mL/1.73 m2/min), and acute inflammation (CRP > 5 mg/dL). The study group was divided in terms of the presence of chronic complications, obesity, or poor metabolic control (HbA1c > 7%). Protein profiling was completed by using the MALDI-TOF MS (matrix-assisted laser desorption/ionization-time of flight mass spectrometry) analyzer. Results: Differentiating proteins were identified in all of the groups. The groups burdened with complications, obesity, and poor metabolic control were characterized by increased levels of fibrinogen, complement C4 and C3. Conclusion: The groups of type 1 diabetes patients burdened with complications, obesity, and poor metabolic control were characterized by increased levels of fibrinogen, complement C4 and C3. Further detailed studies are necessary to determine more subtle changes in the proteomic profile of patients with type 1 diabetes.

ACS Style

Agnieszka Zawada; Dariusz Naskręt; Eliza Matuszewska; Zenon Kokot; Marian Grzymisławski; Dorota Zozulińska-Ziółkiewicz; Agnieszka Dobrowolska; Jan Matysiak. MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications. International Journal of Environmental Research and Public Health 2021, 18, 2263 .

AMA Style

Agnieszka Zawada, Dariusz Naskręt, Eliza Matuszewska, Zenon Kokot, Marian Grzymisławski, Dorota Zozulińska-Ziółkiewicz, Agnieszka Dobrowolska, Jan Matysiak. MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications. International Journal of Environmental Research and Public Health. 2021; 18 (5):2263.

Chicago/Turabian Style

Agnieszka Zawada; Dariusz Naskręt; Eliza Matuszewska; Zenon Kokot; Marian Grzymisławski; Dorota Zozulińska-Ziółkiewicz; Agnieszka Dobrowolska; Jan Matysiak. 2021. "MALDI-TOF Protein Profiling Reflects Changes in Type 1 Diabetes Patients Depending on the Increased Amount of Adipose Tissue, Poor Control of Diabetes and the Presence of Chronic Complications." International Journal of Environmental Research and Public Health 18, no. 5: 2263.

Journal article
Published: 19 February 2021 in International Journal of Molecular Sciences
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Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction.

ACS Style

Magdalena Kulus; Wiesława Kranc; Katarzyna Wojtanowicz-Markiewicz; Piotr Celichowski; Agata Światły-Błaszkiewicz; Eliza Matuszewska; Patrycja Sujka-Kordowska; Aneta Konwerska; Maciej Zdun; Rut Bryl; Maria Wieczorkiewicz; Jakub Kulus; Bogumiła Stelmach; Katarzyna Stefańska; Joanna Budna-Tukan; James Petitte; Paul Mozdziak; Kornel Ratajczak; Jan Matysiak; Jędrzej Jaśkowski; Michał Nowicki; Bartosz Kempisty. New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes. International Journal of Molecular Sciences 2021, 22, 2082 .

AMA Style

Magdalena Kulus, Wiesława Kranc, Katarzyna Wojtanowicz-Markiewicz, Piotr Celichowski, Agata Światły-Błaszkiewicz, Eliza Matuszewska, Patrycja Sujka-Kordowska, Aneta Konwerska, Maciej Zdun, Rut Bryl, Maria Wieczorkiewicz, Jakub Kulus, Bogumiła Stelmach, Katarzyna Stefańska, Joanna Budna-Tukan, James Petitte, Paul Mozdziak, Kornel Ratajczak, Jan Matysiak, Jędrzej Jaśkowski, Michał Nowicki, Bartosz Kempisty. New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes. International Journal of Molecular Sciences. 2021; 22 (4):2082.

Chicago/Turabian Style

Magdalena Kulus; Wiesława Kranc; Katarzyna Wojtanowicz-Markiewicz; Piotr Celichowski; Agata Światły-Błaszkiewicz; Eliza Matuszewska; Patrycja Sujka-Kordowska; Aneta Konwerska; Maciej Zdun; Rut Bryl; Maria Wieczorkiewicz; Jakub Kulus; Bogumiła Stelmach; Katarzyna Stefańska; Joanna Budna-Tukan; James Petitte; Paul Mozdziak; Kornel Ratajczak; Jan Matysiak; Jędrzej Jaśkowski; Michał Nowicki; Bartosz Kempisty. 2021. "New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes." International Journal of Molecular Sciences 22, no. 4: 2082.

Journal article
Published: 13 May 2020 in Metabolites
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Red blood cells (RBCs) are the most abundant cells in the human blood that have been extensively studied under morphology, ultrastructure, biochemical and molecular functions. Therefore, RBCs are excellent cell models in the study of biologically active compounds like drugs and toxins on the structure and function of the cell membrane. The aim of the present study was to explore erythrocyte ghost’s proteome to identify changes occurring under the influence of three bee venom peptides-melittin, tertiapin, and apamin. We conducted preliminary experiments on the erythrocyte ghosts incubated with these peptides at their non-hemolytic concentrations. Such preparations were analyzed using liquid chromatography coupled with tandem mass spectrometry. It was found that when higher concentrations of melittin and apamin were used, fewer proteins were identified. Moreover, the results clearly indicated that apamin demonstrates the greatest influence on the RBCs ghosts proteome. Interestingly, the data also suggest that tertiapin exerted a stabilizing effect on the erythrocyte membrane. The experiments carried out show the great potential of proteomic research in the projects focused on the toxin’s properties as membrane active agents. However, to determine the specificity of the effect of selected bee venom peptides on the erythrocyte ghosts, further proteomic research should be focused on the quantitative analysis.

ACS Style

Agata Światły-Błaszkiewicz; Lucyna Mrówczyńska; Eliza Matuszewska; Jan Lubawy; Arkadiusz Urbański; Zenon J. Kokot; Grzegorz Rosiński; Jan Matysiak. The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study. Metabolites 2020, 10, 191 .

AMA Style

Agata Światły-Błaszkiewicz, Lucyna Mrówczyńska, Eliza Matuszewska, Jan Lubawy, Arkadiusz Urbański, Zenon J. Kokot, Grzegorz Rosiński, Jan Matysiak. The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study. Metabolites. 2020; 10 (5):191.

Chicago/Turabian Style

Agata Światły-Błaszkiewicz; Lucyna Mrówczyńska; Eliza Matuszewska; Jan Lubawy; Arkadiusz Urbański; Zenon J. Kokot; Grzegorz Rosiński; Jan Matysiak. 2020. "The Effect of Bee Venom Peptides Melittin, Tertiapin, and Apamin on the Human Erythrocytes Ghosts: A Preliminary Study." Metabolites 10, no. 5: 191.

Journal article
Published: 27 November 2019 in Allergy, Asthma & Clinical Immunology
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Background Hymenoptera venom allergy is one of the most frequent causes of anaphylaxis. In its most severe form, the reaction to wasp and honey bee stings may be life-threatening. Therefore, immediate and proper diagnosis of venom allergy and implementation of suitable therapy are extremely important. Broadening the knowledge on the mechanism of the allergic reaction may contribute to the improvement of both diagnostic and treatment methods. Thus, this study aimed to discover changes in protein expression in serum of patients allergic to Hymenoptera (wasp and honeybee) venom and to point out proteins and peptides involved in the allergic inflammation. Methods Serum proteomic patterns typical to allergic patients and healthy volunteers were obtained with MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) mass spectrometer. The spectra were processed, analyzed and compared using advanced bioinformatics tools. The discriminative peaks were subjected to identification with liquid chromatography coupled with tandem mass spectrometry. Results This methodology allowed for the identification of four features differentiating between allergy and control groups. They were: fibrinogen alpha chain, coagulation factor XIII chain A, complement C4-A, and inter-alpha-trypsin inhibitor heavy chain H4. All of these proteins are involved in allergic inflammatory response. Conclusions Extending the knowledge of the Hymenoptera venom sensitization will contribute to the development of novel, sensitive and specific methods for quick and unambiguous allergy diagnosis. Understanding the basis of the allergy at the proteomic level will support the improvement of preventive and therapeutic measures.

ACS Style

Eliza Matuszewska; Joanna Matysiak; Anna Bręborowicz; Katarzyna Olejniczak; Zdzisława Kycler; Zenon Kokot; Jan Matysiak. Proteomic features characterization of Hymenoptera venom allergy. Allergy, Asthma & Clinical Immunology 2019, 15, 1 -8.

AMA Style

Eliza Matuszewska, Joanna Matysiak, Anna Bręborowicz, Katarzyna Olejniczak, Zdzisława Kycler, Zenon Kokot, Jan Matysiak. Proteomic features characterization of Hymenoptera venom allergy. Allergy, Asthma & Clinical Immunology. 2019; 15 (1):1-8.

Chicago/Turabian Style

Eliza Matuszewska; Joanna Matysiak; Anna Bręborowicz; Katarzyna Olejniczak; Zdzisława Kycler; Zenon Kokot; Jan Matysiak. 2019. "Proteomic features characterization of Hymenoptera venom allergy." Allergy, Asthma & Clinical Immunology 15, no. 1: 1-8.

Communication
Published: 27 August 2019 in Toxins
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Melittin (MEL) is a basic polypeptide originally purified from honeybee venom. MEL exhibits a broad spectrum of biological activity. However, almost all studies on MEL activity have been carried out on vertebrate models or cell lines. Recently, due to cheap breeding and the possibility of extrapolating the results of the research to vertebrates, insects have been used for various bioassays and comparative physiological studies. For these reasons, it is valuable to examine the influence of melittin on insect physiology. Here, for the first time, we report the immunotropic and cardiotropic effects of melittin on the beetle Tenebrio molitor as a model insect. After melittin injection at 10−7 M and 10−3 M, the number of apoptotic cells in the haemolymph increased in a dose-dependent manner. The pro-apoptotic action of MEL was likely compensated by increasing the total number of haemocytes. However, the injection of MEL did not cause any changes in the percent of phagocytic haemocytes or in the phenoloxidase activity. In an in vitro bioassay with a semi-isolated Tenebrio heart, MEL induced a slight chronotropic-positive effect only at a higher concentration (10−4 M). Preliminary results indicated that melittin exerts pleiotropic effects on the functioning of the immune system and the endogenous contractile activity of the heart. Some of the induced responses in T. molitor resemble the reactions observed in vertebrate models. Therefore, the T. molitor beetle may be a convenient invertebrate model organism for comparative physiological studies and for the identification of new properties and mechanisms of action of melittin and related compounds.

ACS Style

Jan Lubawy; Arkadiusz Urbański; Lucyna Mrówczyńska; Eliza Matuszewska; Agata Światły-Błaszkiewicz; Jan Matysiak; Grzegorz Rosiński. The Influence of Bee Venom Melittin on the Functioning of the Immune System and the Contractile Activity of the Insect Heart—A Preliminary Study. Toxins 2019, 11, 494 .

AMA Style

Jan Lubawy, Arkadiusz Urbański, Lucyna Mrówczyńska, Eliza Matuszewska, Agata Światły-Błaszkiewicz, Jan Matysiak, Grzegorz Rosiński. The Influence of Bee Venom Melittin on the Functioning of the Immune System and the Contractile Activity of the Insect Heart—A Preliminary Study. Toxins. 2019; 11 (9):494.

Chicago/Turabian Style

Jan Lubawy; Arkadiusz Urbański; Lucyna Mrówczyńska; Eliza Matuszewska; Agata Światły-Błaszkiewicz; Jan Matysiak; Grzegorz Rosiński. 2019. "The Influence of Bee Venom Melittin on the Functioning of the Immune System and the Contractile Activity of the Insect Heart—A Preliminary Study." Toxins 11, no. 9: 494.

Journal article
Published: 13 March 2019 in Journal of Medical Science
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To comprehensively characterize honeybee venom, royal jelly, propolis, and pollen, by applying advanced analytical and bioinformatics methodologies. Honeybee products (HBP) contain many bioactive components with both beneficial and harmful effects on the human organism. Nevertheless, the overall composition of the HBP remains not fully investigated. Thus, this research is focused on complementary proteomic and metabolomic characterization of biologically active compounds derived from HBP, regarding their toxicological and pharmacological properties. The objectives of the study will be achieved by the application of up to date mass spectrometry techniques. Due to increasing interest in using of HBP in medicine, this project will contribute to improving the safety of HBP‑derived dietary supplements and drugs.

ACS Style

Eliza Matuszewska; Paweł Dereziński; Agnieszka Klupczyńska; Agata Światły-Błaszkiewicz; Szymon Plewa; Jan Lubawy; Arkadiusz Urbański; Grzegorz Rosiński; Zenon Kokot; Jan Matysiak. Characterization of the selected honeybee products based on omics techniques. Journal of Medical Science 2019, 88, 129 -132.

AMA Style

Eliza Matuszewska, Paweł Dereziński, Agnieszka Klupczyńska, Agata Światły-Błaszkiewicz, Szymon Plewa, Jan Lubawy, Arkadiusz Urbański, Grzegorz Rosiński, Zenon Kokot, Jan Matysiak. Characterization of the selected honeybee products based on omics techniques. Journal of Medical Science. 2019; 88 (2):129-132.

Chicago/Turabian Style

Eliza Matuszewska; Paweł Dereziński; Agnieszka Klupczyńska; Agata Światły-Błaszkiewicz; Szymon Plewa; Jan Lubawy; Arkadiusz Urbański; Grzegorz Rosiński; Zenon Kokot; Jan Matysiak. 2019. "Characterization of the selected honeybee products based on omics techniques." Journal of Medical Science 88, no. 2: 129-132.

Journal article
Published: 12 March 2019 in Journal of Medical Science
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Introduction. High-mobility group box 1 (HMGB1) is an alarmin with proinflammatory potential determined by redox status of the cysteines at position 23 and 45. It may also play a role as a biomarker in biological fluids. The aim of this study was the identification of different HMGB1 redox forms in cerebrospinal fluid (CSF) obtained from subarachnoid hemorrhage patients. Material and Methods. 6 CSF samples were collected from aneurysmal subarachnoid haemorrhage patients. Commercially available HMGB1 isoforms served as a positive control. Immunoprecipitation and electrophoretic isolation of HMGB1 protein were performed, then both CSF and control were analyzed using mass spectrometry technique. To distinguish between fully reduced (thiol group at C23 and C45) and disulfide (disulfide bond connecting C23 and C45) HMGB1 forms, top-down sequencing of the spectra was performed. Results. Top-down sequencing analysis allowed to distinguish between HMGB1 isoforms only in commercially available standard without preceding immunoprecipitation and electrophoresis. MALDI spectra differ i.e. on the fully reduced HMGB1 spectrum fragmentation occurs before and beyond C22, which is not present on the disulfide HMGB1 spectrum. Analysis of HMGB1 isolated from CSF obtained from subarachnoid hemorrhage patients gave no results. Conclusions. Top-down sequencing enables to distinguish between redox forms of HMGB1. Electrophoresis and tryptic digestion cannot precede mass spectrometry analysis of redox forms of HMGB1 due to the reduction of disulfide bonds during these processes. Preferred method of isolation of HMGB1 for direct analysis using top-down sequencing mustn’t include protein digestion or degradation.

ACS Style

Agata Światły; Norbert Wąsik; Joanna Hajduk; Eliza Matuszewska; Paweł Dereziński; Bartosz Sokół; Roman Jankowski; Zenon Kokot; Jan Matysiak. Mass spectrometry analysis of redox forms of High-Mobility Group Box-1 Protein in cerebrospinal fluid: initial experience. Journal of Medical Science 2019, 88, 171 -176.

AMA Style

Agata Światły, Norbert Wąsik, Joanna Hajduk, Eliza Matuszewska, Paweł Dereziński, Bartosz Sokół, Roman Jankowski, Zenon Kokot, Jan Matysiak. Mass spectrometry analysis of redox forms of High-Mobility Group Box-1 Protein in cerebrospinal fluid: initial experience. Journal of Medical Science. 2019; 88 (3):171-176.

Chicago/Turabian Style

Agata Światły; Norbert Wąsik; Joanna Hajduk; Eliza Matuszewska; Paweł Dereziński; Bartosz Sokół; Roman Jankowski; Zenon Kokot; Jan Matysiak. 2019. "Mass spectrometry analysis of redox forms of High-Mobility Group Box-1 Protein in cerebrospinal fluid: initial experience." Journal of Medical Science 88, no. 3: 171-176.

Journal article
Published: 09 February 2019 in Metabolites
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Gestational trophoblastic disease (GTD) is a group of highly aggressive, rare tumors. Human chorionic gonadotropin is a common biomarker used in the diagnosis and monitoring of GTD. To improve our knowledge of the pathology of GTD, we performed protein-peptide profiling on the urine of patients affected with gestational trophoblastic neoplasm (GTN). We analyzed urine samples from patients diagnosed with GTN (n = 26) and from healthy pregnant and non-pregnant controls (n = 17) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Ions were examined in a linear mode over a m/z range of 1000–10,000. All GTN urine samples were analyzed before and after treatment and compared with those of the controls. The statistical analyses included multivariate classification algorithms as well as ROC curves. Urine sample analyses revealed there were significant differences in the composition of the ions between the evaluated groups. Comparing the pre-treatment and group with the pregnant controls, we identified two discriminatory proteins: hemoglobin subunit α (m/z = 1951.81) and complement C4A (m/z = 1895.43). Then, comparing urine samples from the post-treatment cases with those from the non-pregnant controls, we identified the peptides uromodulin fragments (m/z = 1682.34 and 1913.54) and complement C4A (m/z = 1895.43).

ACS Style

Paulina Banach; Paweł Dereziński; Eliza Matuszewska; Jan Matysiak; Hubert Bochyński; Zenon J. Kokot; Ewa Nowak-Markwitz. MALDI-TOF-MS Analysis in the Identification of Urine Proteomic Patterns of Gestational Trophoblastic Disease. Metabolites 2019, 9, 30 .

AMA Style

Paulina Banach, Paweł Dereziński, Eliza Matuszewska, Jan Matysiak, Hubert Bochyński, Zenon J. Kokot, Ewa Nowak-Markwitz. MALDI-TOF-MS Analysis in the Identification of Urine Proteomic Patterns of Gestational Trophoblastic Disease. Metabolites. 2019; 9 (2):30.

Chicago/Turabian Style

Paulina Banach; Paweł Dereziński; Eliza Matuszewska; Jan Matysiak; Hubert Bochyński; Zenon J. Kokot; Ewa Nowak-Markwitz. 2019. "MALDI-TOF-MS Analysis in the Identification of Urine Proteomic Patterns of Gestational Trophoblastic Disease." Metabolites 9, no. 2: 30.