Studies on adverse health effects associated with air pollution mostly focus on individual pollutants. However, the air is a complex medium, and thus epidemiological studies face many challenges and limitations in the multipollutant approach. NO₂ and PM_2.5 have been selected as both originating from combustion processes and are considered to be the main pollutants associated with traffic; moreover, both elicit oxidative stress responses. An answer to the question of whether synergistic or antagonistic health effects of combined pollutants are demonstrated by pollutants monitored in ambient air is not explicit. Among the analyzed studies, only a few revealed statistical significance. Exposure to a single pollutant (PM_2.5 or NO₂) was mostly associated with a small increase in non-accidental mortality (HR:1.01–1.03). PM_2.5 increase of <10 µg/m³ adjusted for NO₂ as well as NO₂ adjusted for PM_2.5 resulted in a slightly lower health risk than a single pollutant. In the case of cardiovascular heart disease, mortality evoked by exposure to PM_2.5 or NO₂ adjusted for NO₂ and PM_2.5, respectively, revealed an antagonistic effect on health risk compared to the single pollutant. Both short- and long-term exposure to PM_2.5 or NO₂ adjusted for NO₂ and PM_2.5, respectively, revealed a synergistic effect appearing as higher mortality from respiratory diseases.

The issue of healthy educational buildings is a global concern because children are particularly at risk of lung damage and infection caused by poor indoor air quality (IAQ). This article presents the results of a preliminary study of the concentration and size distribution of bacterial aerosol in three educational buildings: a preschool, primary school, and high school. Sampling was undertaken in the classrooms with an Andersen six-stage impactor (with aerodynamic cut-off diameters of 7.0, 4.7, 3.3, 2.1, 1.1 and 0.65 μm) during spring 2016 and 2017, as well as the outside of the buildings. After incubation, bioaerosol particles captured on nutrient media on Petri dishes were quantitatively evaluated and qualitatively identified. The highest average concentration of bacterial aerosol was inside the primary school building (2205 CFU/m³), whereas the lowest average concentration of indoor culturable bacteria was observed in the high school building (391 CFU/m³). Using the obtained data, the exposure dose (ED) of the bacterial aerosol was estimated for children attending each educational level. The most frequently occurring species in the sampled bacterial aerosol were Gram-positive cocci in the indoor environment and Gram-positive rod-forming endospores in the outdoor environment.

Indoor air quality (IAQ) in preschools is an important public health challenge. Particular attention should be paid to younger children, because they are more vulnerable to air pollution than higher grade children and because they spend more time indoors. Among air pollutants, particulate matter (PM) is of the greatest interest mainly due to its acute and chronic effects on children’s health. In addition, carbon dioxide (CO₂) levels indicate ventilation conditions. In this paper, we present the concentrations of PM (PM₁, PM_2.5, PM₁₀ and total—TSP) and CO₂ monitored in four naturally ventilated nursery schools located in the area of Gliwice, Poland. The nursery schools were selected to characterize areas with different degrees of urbanization and traffic densities during the winter season. The results indicate the problem of elevated concentrations of PM inside the examined classrooms, as well as that of high levels of CO₂ exceeding 1000 ppm in relation to outdoor air. The characteristics of IAQ were significantly different, both in terms of classroom occupation (younger or older children) and of localization (urban or rural). To evaluate the children’s exposure to poor IAQ, indicators based on air quality guidelines were proposed to rank classrooms according to their hazard on the health of children.

The concentrations and size distributions of culturable bacterial aerosols were measured during spring and winter in outdoor air in Gliwice, Upper Silesia, Poland. This research on culturable bacteria was carried over a period of two years. The samples were collected using a six-stage Andersen cascade impactor (with aerodynamic cut-off diameters of 7.0, 4.7, 3.3, 2.1, 1.1, and 0.65 μm). The results showed that the average concentration of culturable bacterial aerosol was 355 CFU m⁻³ in spring, which was four times higher than during winter (65 CFU m⁻³). Bacterial aerosol concentrations showed the unimodal size distribution with the highest range of 3.3–4.7 μm particles. The seasonal distributions of bacterial aerosol grain clearly indicate that, in winter, the size distribution of particles <7 μm is more “flattened” and is characterized by an increased share of fine fractions and a decreased share of coarse ones. Environmental parameters, such as temperature, UV radiation, relative humidity, wind velocity, as well as PM₁₀ and PM_2.5 concentrations, were measured in order to analyse whether environmental factors had any effect on bacterial aerosols. Statistically, the most important meteorological factors in the viability of airborne bacteria were temperature and UV radiation.

To better understand the relation between children health and indoor air quality, we measured the concentrations of fine particulate matter (PM_2.5) and 11 metals (arsenic, cadmium, chromium, copper, iron, manganese, nickel, lead, antimony, selenium, and zinc) from air samples taken during both winter and spring, and focused on urban and rural area kindergartens of the Upper Silesia Region, Poland, typified by the use of fossil fuels for power and heat purposes. We combined related inhalation intake estimates for children and health effects using separate dose-response approaches for PM_2.5 and metals. Results show that impacts on children from exposure to PM_2.5 was 7.5 min/yr, corresponding to 14 μDALY/yr (DALY: disability-adjusted life years) with 95% confidence interval (CI): 0.3-164 min/yr, which is approximately 10 times lower than cumulative impacts from exposure to the metal components in the PM_2.5 fraction of indoor air (median 76 min/yr; CI: 0.2-4.5 × 10³ min/yr). Highest metal-related health impacts were caused by exposure to hexavalent chromium. The average combined cancer and non-cancer effects for hexavalent chromium were 55 min/yr, corresponding to 104 μDALY/yr, with CI: 0.5 to 8.0 × 10⁴ min/yr. Health impacts on children varied by season and across urban and rural sites, both as functions of varying PM_2.5 metal compositions influenced by indoor and outdoor emission sources. Our study demonstrates the need to consider indoor environments for evaluating health impacts of children, and can assist decision makers to focus on relevant impact reduction and indoor air quality improvement.