In calcareous soil, the significant portion of applied phosphorus (P) fertilizers is adsorbed on the calcite surface and becomes unavailable to plants. Addition of organic amendments with chemical fertilizers can be helpful in releasing the absorbed nutrients from these surfaces. To check out this problem, a field experiment was conducted for two years to determine the effect of P fertilizers and humic acid (HA) in enhancing P availability in soil and their ultimate effect on growth, yield and P uptake of wheat in calcareous soils. The experiment was comprised of five levels of P (0, 45, 67.5, 90 and 112.5 kg P₂O₅ ha⁻¹) as a single superphosphate (SSP) and 2 levels of locally produced humic acid (with and without HA) arranged in a two factorial randomized complete block design (RCBD) with three replications. Wheat plant height, spike length, number of grains per spike, 1000-grain weight, grain, straw and biological yield were significantly improved by the addition of HA with SSP. Very often, the performance of 67.5 kg P₂O₅ ha⁻¹ with HA were either similar or better than 90 or even 112.5 kg P₂O₅ ha⁻¹ applied without HA. Post-harvest soil organic matter, AB-DTPA extractable and water-soluble P, plant P concentration and its uptake were also significantly improved by the addition of HA with SSP compared to sole SSP application. It was evident that P efficiency could be increased with HA addition and it has the potential to improve crop yield and plants P uptake in calcareous soils.