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Chengzhen Liang
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China

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Original article
Published: 30 July 2021 in Planta
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The elevation of transcript levels of GhDREB1B causes the accumulation of osmoregulants and mitigation of reactive oxygen species, which contributes to the enhanced resistance to chilling stress in AiSheng98 cotton. Low temperature is one of the key environmental stresses that impairs cotton growth and restricts fiber productivity. Dehydration responsive element binding (DREB) transcription factors play an important role in cold response in plants by modulating the transcription level of cold-responsive genes to protect the plants from low-temperature stress. Here, we showed that GhDREB1B, a copy number variant in the AiSheng98 (AS98) cotton mutant, significantly improved chilling tolerance in cotton seedlings, while silencing of GhDREB1B made transgenic cotton sensitive to chilling stress in AS98 cotton compared with control plants. Elevated GhDREB1B transcript level activated the expression of major cold-responsive genes. Genome-wide expression profiling by RNA sequencing revealed the upregulation of genes related to fatty acids, lipid proteins, osmoprotection, and anti-oxidative enzymes in AiSheng98. Excessive accumulation of malondialdehyde (MDA) and higher ion leakage rates occurred in wild-type LFH10 plants when compared to those of Aisheng98 during chilling stress, signifying lower chilling tolerance in the wild-type than in Aisheng98. Furthermore, the Aisheng98 mutant under chilling stress accumulated higher levels of free proline and soluble sugar than LFH10 accumulated. These results suggest that GhDREB1B is a positive regulator and its variant can alter the expression patterns of major low-temperature stress-related genes and enhance chilling tolerance in cotton.

ACS Style

Yanan Wang; Yuan Wang; Zhigang Meng; Yunxiao Wei; Xiongming Du; Chengzhen Liang; Rui Zhang. Elevation of GhDREB1B transcription by a copy number variant significantly improves chilling tolerance in cotton. Planta 2021, 254, 1 -10.

AMA Style

Yanan Wang, Yuan Wang, Zhigang Meng, Yunxiao Wei, Xiongming Du, Chengzhen Liang, Rui Zhang. Elevation of GhDREB1B transcription by a copy number variant significantly improves chilling tolerance in cotton. Planta. 2021; 254 (2):1-10.

Chicago/Turabian Style

Yanan Wang; Yuan Wang; Zhigang Meng; Yunxiao Wei; Xiongming Du; Chengzhen Liang; Rui Zhang. 2021. "Elevation of GhDREB1B transcription by a copy number variant significantly improves chilling tolerance in cotton." Planta 254, no. 2: 1-10.

Correction
Published: 11 March 2021 in Toxins
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The authors wish to make the following corrections to their paper

ACS Style

Tahira Syed; Muhammad Askari; Zhigang Meng; Yanyan Li; Muhammad Abid; Yunxiao Wei; Sandui Guo; Chengzhen Liang; Rui Zhang. Correction: Syed, T., et al. Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers. Toxins 2020, 12, 522. Toxins 2021, 13, 200 .

AMA Style

Tahira Syed, Muhammad Askari, Zhigang Meng, Yanyan Li, Muhammad Abid, Yunxiao Wei, Sandui Guo, Chengzhen Liang, Rui Zhang. Correction: Syed, T., et al. Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers. Toxins 2020, 12, 522. Toxins. 2021; 13 (3):200.

Chicago/Turabian Style

Tahira Syed; Muhammad Askari; Zhigang Meng; Yanyan Li; Muhammad Abid; Yunxiao Wei; Sandui Guo; Chengzhen Liang; Rui Zhang. 2021. "Correction: Syed, T., et al. Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers. Toxins 2020, 12, 522." Toxins 13, no. 3: 200.

Journal article
Published: 04 February 2021 in Journal of Hazardous Materials
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High efficiency photocatalysts capable of disinfecting a broad-spectrum microorganisms are needed for the practical application of photodisinfection technology. Herein, we synthesized a highly efficient photodisinfection catalyst composed of Ti3+ self-doped TiO2 decorated with carbon dots (CDs) and palladium nano-photocatalyst, designated as Pd/CDs/Ti3+-TiO2, via a facile hydrothermal-calcination approach. XPS and ESR analyses were performed to verify that the composite contained Ti3+, while TEM imaging and FTIR confirmed that the samples contained CDs. The as synthesized photocatalysts, particularly the 1% Pd/CDs/Ti3+-TiO2 sample, exhibited superior photocatalyzed antibacterial activity to pure TiO2 against E. coli (~6.5 orders of magnitude increase at 30 min). The 1% Pd/CDs/Ti3+-TiO2 photocatalyst also exhibited efficient photodisinfection of five pathogenic agricultural fungi. The dark cytotoxicity of the 1% Pd/CDs/Ti3+-TiO2 nanocomposites was evaluated on HepG2 and Chinese hamster lung (V79) cells via Cell Counting Kit-8 (CCK-8) and found to be minimal. Lastly, the recycling capacity for the photodisinfective activity of the nanocomposites was evaluated and found to be unchanged after five cycles. Four active species were identified as contributing to the photoinduced antimicrobial activity of the catalyst: h+, •O2−, •OH, and e−. Together, our results indicate that Pd/CDs/Ti3+-TiO2 nanocomposites have great potential in agricultural plant pathogen disinfection.

ACS Style

Jingtao Zhang; Shurui Liu; Xueying Wang; Jing Yao; Mengwan Zhai; Bingkun Liu; Chengzhen Liang; Hengzhen Shi. Highly efficient Ti3+ self-doped TiO2 co-modified with carbon dots and palladium nanocomposites for disinfection of bacterial and fungi. Journal of Hazardous Materials 2021, 413, 125318 .

AMA Style

Jingtao Zhang, Shurui Liu, Xueying Wang, Jing Yao, Mengwan Zhai, Bingkun Liu, Chengzhen Liang, Hengzhen Shi. Highly efficient Ti3+ self-doped TiO2 co-modified with carbon dots and palladium nanocomposites for disinfection of bacterial and fungi. Journal of Hazardous Materials. 2021; 413 ():125318.

Chicago/Turabian Style

Jingtao Zhang; Shurui Liu; Xueying Wang; Jing Yao; Mengwan Zhai; Bingkun Liu; Chengzhen Liang; Hengzhen Shi. 2021. "Highly efficient Ti3+ self-doped TiO2 co-modified with carbon dots and palladium nanocomposites for disinfection of bacterial and fungi." Journal of Hazardous Materials 413, no. : 125318.

Regular article
Published: 31 October 2020 in New Phytologist
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Improving yield is a primary mission for cotton (Gossypium hirsutum L.) breeders; development of cultivars with suitable architecture for high planting density (HPDA) can increase yield per unit area. We characterized a natural cotton mutant, AiSheng98 (AS98), which exhibits shorter height, shorter branch length, and more acute branch angle than wild type. A copy number variant at the HPDA locus on Chromosome D12 (HPDA‐D12), encoding a dehydration‐responsive element‐binding (DREB) transcription factor, GhDREB1B, strongly affects plant architecture in the AS98 mutant. We found an association between a tandem duplication of a ~13.5 kb segment in HPDA‐D12 and elevated GhDREB1B expression resulting in the AS98 mutant phenotype. GhDREB1B overexpression confers a significant decrease in plant height and branch length, and reduced branch angle. Our results suggest that fine‐tuning GhDREB1B expression may be a viable engineering strategy for modification of plant architecture favorable to high planting density in cotton.

ACS Style

Gaoxiang Ji; Chengzhen Liang; Yingfan Cai; Zhaoe Pan; Zhigang Meng; Yanyan Li; Yinhua Jia; Yuchen Miao; Xinxin Pei; Wenfang Gong; Xiaoyang Wang; Qiong Gao; Zhen Peng; Liru Wang; Junling Sun; Xiaoli Geng; Pengpeng Wang; Baojun Chen; Peilin Wang; Tao Zhu; Shoupu He; Rui Zhang; Xiongming Du. A copy number variant at the HPDA‐D12 locus confers compact plant architecture in cotton. New Phytologist 2020, 229, 2091 -2103.

AMA Style

Gaoxiang Ji, Chengzhen Liang, Yingfan Cai, Zhaoe Pan, Zhigang Meng, Yanyan Li, Yinhua Jia, Yuchen Miao, Xinxin Pei, Wenfang Gong, Xiaoyang Wang, Qiong Gao, Zhen Peng, Liru Wang, Junling Sun, Xiaoli Geng, Pengpeng Wang, Baojun Chen, Peilin Wang, Tao Zhu, Shoupu He, Rui Zhang, Xiongming Du. A copy number variant at the HPDA‐D12 locus confers compact plant architecture in cotton. New Phytologist. 2020; 229 (4):2091-2103.

Chicago/Turabian Style

Gaoxiang Ji; Chengzhen Liang; Yingfan Cai; Zhaoe Pan; Zhigang Meng; Yanyan Li; Yinhua Jia; Yuchen Miao; Xinxin Pei; Wenfang Gong; Xiaoyang Wang; Qiong Gao; Zhen Peng; Liru Wang; Junling Sun; Xiaoli Geng; Pengpeng Wang; Baojun Chen; Peilin Wang; Tao Zhu; Shoupu He; Rui Zhang; Xiongming Du. 2020. "A copy number variant at the HPDA‐D12 locus confers compact plant architecture in cotton." New Phytologist 229, no. 4: 2091-2103.

Journal article
Published: 05 September 2020 in International Journal of Molecular Sciences
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Allotetraploid cotton (Gossypium hirsutum and Gossypium barbadense) are cultivated worldwide for its white fiber. For centuries, conventional breeding approaches increase cotton yield at the cost of extensive erosion of natural genetic variability. Sea Island cotton (G. barbadense) is known for its superior fiber quality, but show poor adaptability as compared to Upland cotton. Here, in this study, we use ethylmethanesulfonate (EMS) as a mutagenic agent to induce genome-wide point mutations to improve the current germplasm resources of Sea Island cotton and develop diverse breeding lines with improved adaptability and excellent economic traits. We determined the optimal EMS experimental procedure suitable for construction of cotton mutant library. At M6 generation, mutant library comprised of lines with distinguished phenotypes of the plant architecture, leaf, flower, boll, and fiber. Genome-wide analysis of SNP distribution and density in yellow leaf mutant reflected the better quality of mutant library. Reduced photosynthetic efficiency and transmission electron microscopy of yellow leaf mutants revealed the effect of induced mutations at physiological and cellular level. Our mutant collection will serve as the valuable resource for basic research on cotton functional genomics, as well as cotton breeding.

ACS Style

Muhammad Ali Abid; Peilin Wang; Tao Zhu; Chengzhen Liang; Zhigang Meng; Waqas Malik; Sandui Guo; Rui Zhang. Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement. International Journal of Molecular Sciences 2020, 21, 6505 .

AMA Style

Muhammad Ali Abid, Peilin Wang, Tao Zhu, Chengzhen Liang, Zhigang Meng, Waqas Malik, Sandui Guo, Rui Zhang. Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement. International Journal of Molecular Sciences. 2020; 21 (18):6505.

Chicago/Turabian Style

Muhammad Ali Abid; Peilin Wang; Tao Zhu; Chengzhen Liang; Zhigang Meng; Waqas Malik; Sandui Guo; Rui Zhang. 2020. "Construction of Gossypiumbarbadense Mutant Library Provides Genetic Resources for Cotton Germplasm Improvement." International Journal of Molecular Sciences 21, no. 18: 6505.

Review
Published: 14 August 2020 in Toxins
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Bacillus thuringiensis (Bt) is a gram negative soil bacterium. This bacterium secretes various proteins during different growth phases with an insecticidal potential against many economically important crop pests. One of the important families of Bt proteins is vegetative insecticidal proteins (Vip), which are secreted into the growth medium during vegetative growth. There are three subfamilies of Vip proteins. Vip1 and Vip2 heterodimer toxins have an insecticidal activity against many Coleopteran and Hemipteran pests. Vip3, the most extensively studied family of Vip toxins, is effective against Lepidopteron. Vip proteins do not share homology in sequence and binding sites with Cry proteins, but share similarities at some points in their mechanism of action. Vip3 proteins are expressed as pyramids alongside Cry proteins in crops like maize and cotton, so as to control resistant pests and delay the evolution of resistance. Biotechnological- and in silico-based analyses are promising for the generation of mutant Vip proteins with an enhanced insecticidal activity and broader spectrum of target insects.

ACS Style

Tahira Syed; Muhammad Askari; Zhigang Meng; Yanyan Li; Muhammad Abid; Yunxiao Wei; Sandui Guo; Chengzhen Liang; Rui Zhang. Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers. Toxins 2020, 12, 522 .

AMA Style

Tahira Syed, Muhammad Askari, Zhigang Meng, Yanyan Li, Muhammad Abid, Yunxiao Wei, Sandui Guo, Chengzhen Liang, Rui Zhang. Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers. Toxins. 2020; 12 (8):522.

Chicago/Turabian Style

Tahira Syed; Muhammad Askari; Zhigang Meng; Yanyan Li; Muhammad Abid; Yunxiao Wei; Sandui Guo; Chengzhen Liang; Rui Zhang. 2020. "Current Insights on Vegetative Insecticidal Proteins (Vip) as Next Generation Pest Killers." Toxins 12, no. 8: 522.

Journal article
Published: 16 July 2020 in Genes
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Cotton is an important economic crop affected by different abiotic stresses at different developmental stages. Salinity limits the growth and productivity of crops worldwide. Na+/H+ antiporters play a key role during the plant development and in its tolerance to salt stress. The aim of the present study was a genome-wide characterization and expression pattern analysis under the salinity stress of the sodium-proton antiporter (NHX) of Gossypium barbadense in comparison with Gossypium hirsutum. In G. barbadense, 25 NHX genes were identified on the basis of the Na+_H+ exchanger domain. All except one of the G. barbadense NHX transporters have an Amiloride motif that is a known inhibitor of Na+ ions in plants. A phylogenetic analysis inferred three classes of GbNHX genes—viz., Vac (GbNHX1, 2 and 4), Endo (GbNHX6), and PM (GbNHX7). A high number of the stress-related cis-acting elements observed in promoters show their role in tolerance against abiotic stresses. The Ka/Ks values show that the majority of GbNHX genes are subjected to strong purifying selection under the course of evolution. To study the functional divergence of G. barbadense NHX transporters, the real-time gene expression was analyzed under salt stress in the root, stem, and leaf tissues. In G. barbadense, the expression was higher in the stem, while in G. hirsutum the leaf and root showed a high expression. Moreover, our results revealed that NHX2 homologues in both species have a high expression under salinity stress at higher time intervals, followed by NHX7. The protein-protein prediction study revealed that GbNHX7 is involved in the CBL-CIPK protein interaction pathway. Our study also provided valuable information explaining the molecular mechanism of Na+ transport for the further functional study of Gossypium NHX genes.

ACS Style

Umar Akram; Yuhan Song; Chengzhen Liang; Muhammad Ali Abid; Muhammad Askari; Aye Aye Myat; Mubashir Abbas; Waqas Malik; Zulfiqar Ali; Sandui Guo; Rui Zhang; Zhigang Meng. Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress in Gossypium barbadense and Its Comparison with Gossypium hirsutum. Genes 2020, 11, 803 .

AMA Style

Umar Akram, Yuhan Song, Chengzhen Liang, Muhammad Ali Abid, Muhammad Askari, Aye Aye Myat, Mubashir Abbas, Waqas Malik, Zulfiqar Ali, Sandui Guo, Rui Zhang, Zhigang Meng. Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress in Gossypium barbadense and Its Comparison with Gossypium hirsutum. Genes. 2020; 11 (7):803.

Chicago/Turabian Style

Umar Akram; Yuhan Song; Chengzhen Liang; Muhammad Ali Abid; Muhammad Askari; Aye Aye Myat; Mubashir Abbas; Waqas Malik; Zulfiqar Ali; Sandui Guo; Rui Zhang; Zhigang Meng. 2020. "Genome-Wide Characterization and Expression Analysis of NHX Gene Family under Salinity Stress in Gossypium barbadense and Its Comparison with Gossypium hirsutum." Genes 11, no. 7: 803.

Journal article
Published: 14 November 2018 in Journal of Integrative Agriculture
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Leaf is a essential part of the plants for photosynthetic activities which mainly economize the resources for boll heath. Significant variations of leaf shapes across the Gossypium sp. considerably influence the infiltration of sunlight for photosynthesis. To understand the genetic variants and molecular processes underlying for cotton leaf shape, we used F2 population derived from upland cotton genotype P30A (shallow-lobed leaf) and sea-island cotton genotype ISR (deep-lobed leaf) to map leaf deep lobed phenotype controlling genes LBL1 and LBL2. Genetic analysis and localization results have unmasked the position and interaction between both loci of LBL1 and LBL2, and revealed the co-dominance impact of the genes in regulating depth of leaf blades lobes in cotton. LBL1 had been described as a main gene and member of transcription factor family leucine zipper (HD-ZIPI) from a class I homologous domain factor Gorai.002G244000. The qRT-PCR results elaborated the continuous change in expression level of LBL1 at different growth stages and leaf parts of cotton. Higher expression level was observed in mature large leaves followed by medium and young leaves respectively. For further confirmation, plants were tested from hormonal induction treatments, which explained that LBL1 expression was influenced by hormonal signaling. Moreover, the highest expression level was detected in brassinolides (BR) treatment as compared to other hormones, and this hormone plays an important role in the process of leaf blade lobed formation.

ACS Style

Dao-Fang He; Xiang Zhao; Cheng-Zhen Liang; Tao Zhu; Muhammad Ali Abid; Yong-Ping Cai; Jin-Ling He; Rui Zhang. Genetic variation in LBL1 contributes to depth of leaf blades lobes between cotton subspecies, Gossypium barbadense and Gossypium hirsutum. Journal of Integrative Agriculture 2018, 17, 2394 -2404.

AMA Style

Dao-Fang He, Xiang Zhao, Cheng-Zhen Liang, Tao Zhu, Muhammad Ali Abid, Yong-Ping Cai, Jin-Ling He, Rui Zhang. Genetic variation in LBL1 contributes to depth of leaf blades lobes between cotton subspecies, Gossypium barbadense and Gossypium hirsutum. Journal of Integrative Agriculture. 2018; 17 (11):2394-2404.

Chicago/Turabian Style

Dao-Fang He; Xiang Zhao; Cheng-Zhen Liang; Tao Zhu; Muhammad Ali Abid; Yong-Ping Cai; Jin-Ling He; Rui Zhang. 2018. "Genetic variation in LBL1 contributes to depth of leaf blades lobes between cotton subspecies, Gossypium barbadense and Gossypium hirsutum." Journal of Integrative Agriculture 17, no. 11: 2394-2404.

Software
Published: 14 November 2017 in BMC Bioinformatics
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The traditional method of visualizing gene annotation data in JBrowse is converting GFF3 files to JSON format, which is time-consuming. The latest version of JBrowse supports rendering sorted GFF3 files indexed by tabix, a novel strategy that is more convenient than the original conversion process. However, current tools available for GFF3 file sorting have some limitations and their sorting results would lead to erroneous rendering in JBrowse. We developed GFF3sort, a script to sort GFF3 files for tabix indexing. Specifically designed for JBrowse rendering, GFF3sort can properly deal with the order of features that have the same chromosome and start position, either by remembering their original orders or by conducting parent-child topology sorting. Based on our test datasets from seven species, GFF3sort produced accurate sorting results with acceptable efficiency compared with currently available tools. GFF3sort is a novel tool to sort GFF3 files for tabix indexing. We anticipate that GFF3sort will be useful to help with genome annotation data processing and visualization. The online version of this article (10.1186/s12859-017-1930-3) contains supplementary material, which is available to authorized users.

ACS Style

Tao Zhu; Chengzhen Liang; Zhigang Meng; Sandui Guo; Rui Zhang. GFF3sort: a novel tool to sort GFF3 files for tabix indexing. BMC Bioinformatics 2017, 18, 482 .

AMA Style

Tao Zhu, Chengzhen Liang, Zhigang Meng, Sandui Guo, Rui Zhang. GFF3sort: a novel tool to sort GFF3 files for tabix indexing. BMC Bioinformatics. 2017; 18 (1):482.

Chicago/Turabian Style

Tao Zhu; Chengzhen Liang; Zhigang Meng; Sandui Guo; Rui Zhang. 2017. "GFF3sort: a novel tool to sort GFF3 files for tabix indexing." BMC Bioinformatics 18, no. 1: 482.

Other
Published: 30 August 2017
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SummaryDesigning specific primers for multiple sites across the whole genome is still challenging, especially in species with complex genomes. Here we present PrimerServer, a high-throughput primer design and specificity-checking platform with both web and command-line interfaces. This platform efficiently integrates site selection, primer design, specificity checking and data presentation. In our case study, PrimerServer achieved high accuracy and a fast running speed for a large number of sites, suggesting its potential for molecular biology applications such as molecular breeding or medical testing.Availability and ImplementationSource code for PrimerServer is available at https://github.com/billzt/PrimerServer. A demo server is freely accessible at https://primerserver.org, with all major browsers [email protected] or [email protected]

ACS Style

Tao Zhu; Chengzhen Liang; Zhigang Meng; Yanyan Li; Yayu Wu; Sandui Guo; Rui Zhang. PrimerServer: a high-throughput primer design and specificity-checking platform. 2017, 181941 .

AMA Style

Tao Zhu, Chengzhen Liang, Zhigang Meng, Yanyan Li, Yayu Wu, Sandui Guo, Rui Zhang. PrimerServer: a high-throughput primer design and specificity-checking platform. . 2017; ():181941.

Chicago/Turabian Style

Tao Zhu; Chengzhen Liang; Zhigang Meng; Yanyan Li; Yayu Wu; Sandui Guo; Rui Zhang. 2017. "PrimerServer: a high-throughput primer design and specificity-checking platform." , no. : 181941.

Preprint
Published: 04 June 2017
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Background:The traditional method of visualizing gene annotation data in JBrowse is converting GFF3 files to JSON format, which is time-consuming. The latest version of JBrowse supports rendering sorted GFF3 files indexed by tabix, a novel strategy that is more convenient than the original conversion process. However, current tools available for GFF3 file sorting have some limitations and their sorting results would lead to erroneous rendering in JBrowse.Results:We developed GFF3sort, a script to sort GFF3 files for tabix indexing. Specifically designed for JBrowse rendering, GFF3sort can properly deal with the order of features that have the same chromosome and start position, either by remembering their original orders or by conducting parent-child topology sorting. Based on our test datasets from seven species, GFF3sort produced accurate sorting results with acceptable efficiency compared with currently available tools.Conclusions:GFF3sort is a novel tool to sort GFF3 files for tabix indexing. We anticipate that GFF3sort will be useful to help with genome annotation data processing and visualization.

ACS Style

Tao Zhu; Chengzhen Liang; Zhigang Meng; Sandui Guo; Rui Zhang. GFF3sort: a novel tool to sort GFF3 files for tabix indexing. 2017, 145938 .

AMA Style

Tao Zhu, Chengzhen Liang, Zhigang Meng, Sandui Guo, Rui Zhang. GFF3sort: a novel tool to sort GFF3 files for tabix indexing. . 2017; ():145938.

Chicago/Turabian Style

Tao Zhu; Chengzhen Liang; Zhigang Meng; Sandui Guo; Rui Zhang. 2017. "GFF3sort: a novel tool to sort GFF3 files for tabix indexing." , no. : 145938.

English abstract
Published: 01 January 2012 in Sheng Wu Gong Cheng Xue Bao
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ACS Style

Xiao Zhang; Rui Zhang; Guoqing Sun; Ji Shi; Zhigang Meng; Tao Zhou; Siyu Hou; Chengzhen Liang; Yuanhua Yu; Sandui Guo. [High efficiency genome walking method for flanking sequences of cotton mitochondrial double-copy atpA gene based on optimized inverse PCR and TAIL-PCR]. Sheng Wu Gong Cheng Xue Bao 2012, 28, 1 .

AMA Style

Xiao Zhang, Rui Zhang, Guoqing Sun, Ji Shi, Zhigang Meng, Tao Zhou, Siyu Hou, Chengzhen Liang, Yuanhua Yu, Sandui Guo. [High efficiency genome walking method for flanking sequences of cotton mitochondrial double-copy atpA gene based on optimized inverse PCR and TAIL-PCR]. Sheng Wu Gong Cheng Xue Bao. 2012; 28 (1):1.

Chicago/Turabian Style

Xiao Zhang; Rui Zhang; Guoqing Sun; Ji Shi; Zhigang Meng; Tao Zhou; Siyu Hou; Chengzhen Liang; Yuanhua Yu; Sandui Guo. 2012. "[High efficiency genome walking method for flanking sequences of cotton mitochondrial double-copy atpA gene based on optimized inverse PCR and TAIL-PCR]." Sheng Wu Gong Cheng Xue Bao 28, no. 1: 1.