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Dr. Lidia Stasiak-Różanska
Institute of Food Sciences, Department of Food Technology and Assessment, Warsaw University of Life Sciences - SGGW, 02-787 Warszawa, Poland

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Research Keywords & Expertise

0 Essential Oils
0 Fermentation
0 Food Microbiology
0 Food Technology
0 Probiotics

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Acetic bacteria
Utilization
Lactic bacteria
Essential Oils
Probiotics
antimicrobial activity
bacterial cellulose
Microbial cellulose

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Journal article
Published: 27 January 2021 in International Journal of Environmental Research and Public Health
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Probiotics are recommended, among others, in the diet of children who are under antibiotic therapy, or that suffer from food allergies or travel diarrhea, etc. In the case of toddlers taking probiotic preparations, it is highly recommended to first remove the special capsule, which normally protects probiotic strains against hard conditions in the gastrointestinal tract. Otherwise, the toddler may choke. This removal can impair probiotic survival and reduce its efficacy in a toddler’s organism. The aim of this study was to evaluate the survivability of five strains of lactic acid bacteria from the commercial probiotics available on the Polish market under simulated conditions of the gastrointestinal tract. Five probiotics (each including one of these strains: Bifidobacterium BB-12, Lactobacillus (Lb.) rhamnosus GG, Lb. casei, Lb. acidophilus, Lb. plantarum) were protective capsule deprived, added in a food matrix (chicken–vegetable soup) and subjected under simulated conditions of the gastric and gastrointestinal passage. Strain survivability and possibility to growth were evaluated. Obtained results showed that, among all analyzed commercial probiotic strains, the Lb. plantarum was the most resistant to the applied conditions of the culture medium. They showed a noticeable growth under both in vitro gastric conditions at pH 4.0 and 5.0, as well as in vitro intestinal conditions at all tested concentrations of bile salts.

ACS Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Antoni Pluta; Krzysztof Dasiewicz; Monika Garbowska. Effect of Simulated Gastrointestinal Tract Conditions on Survivability of Probiotic Bacteria Present in Commercial Preparations. International Journal of Environmental Research and Public Health 2021, 18, 1108 .

AMA Style

Lidia Stasiak-Różańska, Anna Berthold-Pluta, Antoni Pluta, Krzysztof Dasiewicz, Monika Garbowska. Effect of Simulated Gastrointestinal Tract Conditions on Survivability of Probiotic Bacteria Present in Commercial Preparations. International Journal of Environmental Research and Public Health. 2021; 18 (3):1108.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Antoni Pluta; Krzysztof Dasiewicz; Monika Garbowska. 2021. "Effect of Simulated Gastrointestinal Tract Conditions on Survivability of Probiotic Bacteria Present in Commercial Preparations." International Journal of Environmental Research and Public Health 18, no. 3: 1108.

Journal article
Published: 24 July 2020 in Polymers
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Biocellulose, named “the biomaterial of the future”, is a natural and ecologically friendly polymer, produced by selected acetic acid bacteria strains. Biocellulose impregnated with antimicrobial agents can be used as a novel, safe, and biodegradable food packaging material, helping extend the shelf life of some products and may also have the chance to replace typical plastic packaging, which is a big environmental problem these days. This study aimed to evaluate if cellulose impregned with natural oregano essential oil could show antibacterial activity against Cronobacter strains, which can occur in food, causing diseases and food poisoning. Bacterial cellulose was obtained from two acetic bacteria strains, Gluconacetobacter hansenii ATCC 23769 and Komagataeibacter sp. GH1. Antibacterial activity was studied by the disc-diffusion method against chosen Cronobacter strains, isolated from the plant matrix. Oregano essential oil has been shown to penetrate into the structure of bacterial cellulose, and after applying cellulose to the solid medium, it showed the ability to migrate. Biopolymer from the strain K. sp. GH1 was able to better absorb and retain essential oregano oil (OEO) compared to bacterial cellulose (BC) produced by the G. hansenii ATCC 23769. Bacterial cellulose with oregano essential oil from strain Komagataeibacter GH1 showed generally greater inhibitory properties for the growth of tested strains than its equivalent obtained from G. hansenii. This was probably due to the arrangement of the polymer fibers and its final thickness. The largest zone of inhibition of strain growth was observed in relation to C. condimenti s37 (32.75 mm ± 2.8). At the same time, the control sample using filter paper showed an inhibition zone of 36.0 mm ± 0.7. A similar inhibition zone (28.33 mm ± 2.6) was observed for the C. malonaticus lv31 strain, while the zone in the control sample was 27.1 mm ± 0.7. Based on this study, it was concluded that bacterial cellulose impregnated with oregano essential oil has strong and moderate antimicrobial activity against all presented strains of the genus Cronobacter isolated from plant matrix. Obtained results give a strong impulse to use this biopolymer as ecological food packaging in the near future.

ACS Style

Gulden Nagmetova; Anna Berthold-Pluta; Monika Garbowska; Askar Kurmanbayev; Lidia Stasiak-Różańska. Antibacterial Activity of Biocellulose with Oregano Essential Oil against Cronobacter Strains. Polymers 2020, 12, 1647 .

AMA Style

Gulden Nagmetova, Anna Berthold-Pluta, Monika Garbowska, Askar Kurmanbayev, Lidia Stasiak-Różańska. Antibacterial Activity of Biocellulose with Oregano Essential Oil against Cronobacter Strains. Polymers. 2020; 12 (8):1647.

Chicago/Turabian Style

Gulden Nagmetova; Anna Berthold-Pluta; Monika Garbowska; Askar Kurmanbayev; Lidia Stasiak-Różańska. 2020. "Antibacterial Activity of Biocellulose with Oregano Essential Oil against Cronobacter Strains." Polymers 12, no. 8: 1647.

Journal article
Published: 21 September 2019 in Molecules
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The aim of this work was to analyse the effect of ultrasound-assisted osmotic dehydration of apples v. Elise on mass transfer parameters, water activity, and colour changes. Ultrasound treatment was performed at a frequency of 21 kHz with a temperature of 40 °C for 30–180 min using four osmotic solutions: 30% concentrated syrups of erythritol, xylitol, maltitol, and dihydroxyacetone (DHA). The efficiency of the used solutes from the polyol groups was compared to reference dehydration in 50% concentrated sucrose solution. Peleg’s model was used to fit experimental data. Erythritol, xylitol, and DHA solutions showed similar efficiency to sucrose and good water removal properties in compared values of true water loss. The application of ultrasound by two methods was in most cases unnoticeable and weaker than was expected. On the other hand, sonication by the continuous method allowed for a significant reduction in water activity in apple tissue in all tested solutions.

ACS Style

Joanna Cichowska; Dorota Witrowa-Rajchert; Lidia Stasiak-Różańska; Adam Figiel. Ultrasound-Assisted Osmotic Dehydration of Apples in Polyols and Dihydroxyacetone (DHA) Solutions. Molecules 2019, 24, 3429 .

AMA Style

Joanna Cichowska, Dorota Witrowa-Rajchert, Lidia Stasiak-Różańska, Adam Figiel. Ultrasound-Assisted Osmotic Dehydration of Apples in Polyols and Dihydroxyacetone (DHA) Solutions. Molecules. 2019; 24 (19):3429.

Chicago/Turabian Style

Joanna Cichowska; Dorota Witrowa-Rajchert; Lidia Stasiak-Różańska; Adam Figiel. 2019. "Ultrasound-Assisted Osmotic Dehydration of Apples in Polyols and Dihydroxyacetone (DHA) Solutions." Molecules 24, no. 19: 3429.

Journal article
Published: 20 May 2019 in Postępy Mikrobiologii - Advancements of Microbiology
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ACS Style

Lidia Stasiak-Różańska; Milena Kupiec. INDUSTRIAL APPLICATIONS OF WILD AND GENETICALLY-MODIFIED STRAINS OF ACETIC ACID BACTERIA. Postępy Mikrobiologii - Advancements of Microbiology 2019, 57, 398 -402.

AMA Style

Lidia Stasiak-Różańska, Milena Kupiec. INDUSTRIAL APPLICATIONS OF WILD AND GENETICALLY-MODIFIED STRAINS OF ACETIC ACID BACTERIA. Postępy Mikrobiologii - Advancements of Microbiology. 2019; 57 (4):398-402.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Milena Kupiec. 2019. "INDUSTRIAL APPLICATIONS OF WILD AND GENETICALLY-MODIFIED STRAINS OF ACETIC ACID BACTERIA." Postępy Mikrobiologii - Advancements of Microbiology 57, no. 4: 398-402.

Journal article
Published: 09 January 2019 in Foods
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The purpose of this paper is twofold: on the one hand, we verify effectiveness of alternatives solutes to sucrose solution as osmotic agents, while on the other hand we intend to analyze modeling transfer parameters, using different models. There has also been proposed a new mass transfer parameter—true water loss, which includes actual solid gain during the process. Additional consideration of a new ratio (Cichowska et al. Ratio) can be useful for better interpretation of osmotic dehydration (OD) in terms of practical applications. Apples v. Elise were dipped into 30% concentrated solutions of erythritol, xylitol, maltitol, and dihydroxyacetone (DHA) to remove some water from the tissue. To evaluate the efficiency of these solutes, 50% concentrated sucrose solution was used as a control. All of the tested osmotic agent, except maltitol, were effective in the process as evidenced by high values in the true water loss parameter. Solutions of erythritol and xylitol in 30% concentrate could be an alternative to sucrose in the process of osmotic dehydration. Peleg’s, Kelvin–Voigt, and Burgers models could fit well with the experimental data. modeling of mass transfer parameters, using Peleg’s model can be satisfactorily supplemented by Kelvin–Voigt and Burgers model for better prediction of OD within the particular periods of the process.

ACS Style

Joanna Cichowska; Adam Figiel; Lidia Stasiak-Różańska; Dorota Witrowa-Rajchert. Modeling of Osmotic Dehydration of Apples in Sugar Alcohols and Dihydroxyacetone (DHA) Solutions. Foods 2019, 8, 20 .

AMA Style

Joanna Cichowska, Adam Figiel, Lidia Stasiak-Różańska, Dorota Witrowa-Rajchert. Modeling of Osmotic Dehydration of Apples in Sugar Alcohols and Dihydroxyacetone (DHA) Solutions. Foods. 2019; 8 (1):20.

Chicago/Turabian Style

Joanna Cichowska; Adam Figiel; Lidia Stasiak-Różańska; Dorota Witrowa-Rajchert. 2019. "Modeling of Osmotic Dehydration of Apples in Sugar Alcohols and Dihydroxyacetone (DHA) Solutions." Foods 8, no. 1: 20.

Original paper
Published: 28 December 2018 in European Food Research and Technology
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Essential oils (EOs) are liquid preparations produced from plant materials. Their use as inhibitors of the growth of spoilage and pathogenic microorganisms is a good alternative to the chemical additives in foods. The disc-diffusion method was used to screen the EOs from thyme, cinnamon, clove, peppermint, marjoram, cumin, rosemary, fennel, basil, lime, bergamot orange, orange, lemon, grapefruit, mandarin, cardamom, anise, and ginger, against 21 strains of Cronobacter species, including: C. sakazakii, C. muytjensii, C. turicensis, C. condimenti, and C. malonaticus. In addition, the minimum inhibitory concentration (MIC) and the maximum tolerable concentration (MTC) of thymol, trans-cinnamaldehyde, eugenol, and menthol were determined for five strains of Cronobacter spp. The most effective EOs were: thyme > cinnamon > marjoram. In turn, EOs from clove, cumin, and fennel had a moderate inhibiting effect against only some of the analyzed strains. The majority of the tested EOs: peppermint, rosemary, basil, cardamom, anise, ginger, and all EOs from citrus fruits were ineffective against all the studied strains.

ACS Style

Anna Berthold-Pluta; Lidia Stasiak-Różańska; Antoni Pluta; Monika Garbowska. Antibacterial activities of plant-derived compounds and essential oils against Cronobacter strains. European Food Research and Technology 2018, 245, 1137 -1147.

AMA Style

Anna Berthold-Pluta, Lidia Stasiak-Różańska, Antoni Pluta, Monika Garbowska. Antibacterial activities of plant-derived compounds and essential oils against Cronobacter strains. European Food Research and Technology. 2018; 245 (5):1137-1147.

Chicago/Turabian Style

Anna Berthold-Pluta; Lidia Stasiak-Różańska; Antoni Pluta; Monika Garbowska. 2018. "Antibacterial activities of plant-derived compounds and essential oils against Cronobacter strains." European Food Research and Technology 245, no. 5: 1137-1147.

Journal article
Published: 06 December 2018 in Applied Sciences
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Waste glycerol is the main by-product generated during biodiesel production, in an amount reaching up to 10% of the produced biofuel. Is there any method which allows changing this waste into industrial valuable compounds? This manuscript describes a method for valorization of crude glycerol via microbial bioconversion. It has been shown that the use of free and immobilized biocatalysts obtained from Gluconobacter oxydans can enable beneficial valorization of crude glycerol to industrially valuable dihydroxyacetone. The highest concentration of this compound, reaching over 20 g·L−1, was obtained after 72 h of biotransformation with free G. oxydans cells, in a medium containing 30 or 50 g·L−1 of waste glycerol. Using a free cell extract resulted in higher concentrations of dihydroxyacetone and a higher valorization efficiency (up to 98%) compared to the reaction with an immobilized cell extract. Increasing waste glycerol concentration to 50 g·L−1 causes neither a faster nor higher increase in product yield and reaction efficiency compared to its initial concentration of 30 g·L−1. The proposed method could be an alternative for utilization of a petrochemical waste into industry applicated chemicals.

ACS Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Pritam Kumar Dikshit. Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from Gluconobacter oxydans. Applied Sciences 2018, 8, 2517 .

AMA Style

Lidia Stasiak-Różańska, Anna Berthold-Pluta, Pritam Kumar Dikshit. Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from Gluconobacter oxydans. Applied Sciences. 2018; 8 (12):2517.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Pritam Kumar Dikshit. 2018. "Valorization of Waste Glycerol to Dihydroxyacetone with Biocatalysts Obtained from Gluconobacter oxydans." Applied Sciences 8, no. 12: 2517.

Journal article
Published: 01 September 2018 in Zeszyty Problemowe Postępów Nauk Rolniczych
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ACS Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Anna Bzducha-Wróbel. Effect of the additive of pyrroloquinoline quinone on waste glycerol bioconversion to dihydroxyacetone. Zeszyty Problemowe Postępów Nauk Rolniczych 2018, 59 -67.

AMA Style

Lidia Stasiak-Różańska, Anna Berthold-Pluta, Anna Bzducha-Wróbel. Effect of the additive of pyrroloquinoline quinone on waste glycerol bioconversion to dihydroxyacetone. Zeszyty Problemowe Postępów Nauk Rolniczych. 2018; (594):59-67.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Anna Bzducha-Wróbel. 2018. "Effect of the additive of pyrroloquinoline quinone on waste glycerol bioconversion to dihydroxyacetone." Zeszyty Problemowe Postępów Nauk Rolniczych , no. 594: 59-67.

Journal article
Published: 01 September 2018 in Zeszyty Problemowe Postępów Nauk Rolniczych
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ACS Style

Anna Berthold-Pluta; Monika Garbowska; Antoni Pluta; Lidia Stasiak-Różańska. Members of the Cronobacter genus – characteristics and prevalence in food. Zeszyty Problemowe Postępów Nauk Rolniczych 2018, 3 -15.

AMA Style

Anna Berthold-Pluta, Monika Garbowska, Antoni Pluta, Lidia Stasiak-Różańska. Members of the Cronobacter genus – characteristics and prevalence in food. Zeszyty Problemowe Postępów Nauk Rolniczych. 2018; (594):3-15.

Chicago/Turabian Style

Anna Berthold-Pluta; Monika Garbowska; Antoni Pluta; Lidia Stasiak-Różańska. 2018. "Members of the Cronobacter genus – characteristics and prevalence in food." Zeszyty Problemowe Postępów Nauk Rolniczych , no. 594: 3-15.

Journal article
Published: 14 April 2018 in Polymers
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Can microbial cellulose (MC) be used as a bio-carrier for 1,3-dihydroxy-2-propanone (DHA)? The aim of this study was to examine the possibility of using MC as a biomaterial for DHA transferring into the stratum corneum and inducing changes in skin color. The MC patches were obtained from Gluconacetobacter xylinus strain and incubated in solutions with various concentrations of DHA (g·L−1: 20; 50; 80; 110) at 22 °C for 24 h. Afterwards; the patches were applied onto the skin for 15, 30, or 60 min. Skin color changes were assessed visually compared to a control patches without DHA. The intensity of skin color was increasing with the increase of DHA concentration and time of patches application. Application of MC patches with DHA (50 g·L−1) for 30 min ensured the color which was considered the closest to the desired natural tan effect. MC patches containing DHA can be biocarriers enabling DHA transport into the stratum corneum and causing skin color changes. Study results indicate a new possibility for industrial applications of MC; e.g., as a biocarrier in masking the symptoms of vitiligo or production of self-tanning agents in the form of masks.

ACS Style

Lidia Stasiak-Różańska; Justyna Płoska. Study on the Use of Microbial Cellulose as a Biocarrier for 1,3-Dihydroxy-2-Propanone and Its Potential Application in Industry. Polymers 2018, 10, 438 .

AMA Style

Lidia Stasiak-Różańska, Justyna Płoska. Study on the Use of Microbial Cellulose as a Biocarrier for 1,3-Dihydroxy-2-Propanone and Its Potential Application in Industry. Polymers. 2018; 10 (4):438.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Justyna Płoska. 2018. "Study on the Use of Microbial Cellulose as a Biocarrier for 1,3-Dihydroxy-2-Propanone and Its Potential Application in Industry." Polymers 10, no. 4: 438.

Journal article
Published: 01 May 2017 in Electronic Journal of Biotechnology
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Background: Depletion of petroleum resources has enforced the search for alternative sources of renewable energy. Introduction of biofuels into the market was expected to become a solution to this disadvantageous situation. Attempts to cover fuel demand have, however, caused another severe problem—the waste glycerol generated during biodiesel production at a concentration of approximately 10% w/w. This, in turn, prompted a global search for effective methods of valorization of the waste fraction of glycerol. Results: Utilization of the waste fraction at 48 h with an initial glycerol concentration of 30 g·L-1 and proceeding with 62% efficiency enabled the production of 9 g·L-1 dihydroxyacetone at 50% substrate consumption. The re-use of the immobilized biocatalyst resulted in a similar concentration of dihydroxyacetone (8.7 g·L-1) in two-fold shorter time, with an efficiency of 85% and lower substrate consumption (35%). Conclusions: The method proposed in this work is based on the conversion of waste glycerol to dihydroxyacetone in a reaction catalyzed by immobilized Gluconobacter oxydans cell extract with glycerol dehydrogenase activity, and it could be an effective way to convert waste glycerol into a valuable product

ACS Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel; Edyta Lipińska. Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract. Electronic Journal of Biotechnology 2017, 27, 44 -48.

AMA Style

Lidia Stasiak-Różańska, Stanisław Błażejak, Iwona Gientka, Anna Bzducha-Wróbel, Edyta Lipińska. Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract. Electronic Journal of Biotechnology. 2017; 27 ():44-48.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel; Edyta Lipińska. 2017. "Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract." Electronic Journal of Biotechnology 27, no. : 44-48.

Journal article
Published: 14 July 2016 in European Food Research and Technology
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Single cell oils (SCO) can be a source of nutritionally valuable fatty acids and raw material in biodiesel production. Among the oleaginous yeasts, those which additionally generate carotenoid pigments are beneficially distinguished. Rhodotorula and Sporobolomyces strains were screened for the ability to grow and produce intracellular lipids in medium containing pure glycerol. In this paper, we verified that glycerol is unfavorable carbon source for a strain of S. salmonicolor and a good source of carbon for the growth and increases lipids content in the biomass of examined Rhodotorula yeast compared to glucose. The best volumetric efficiency of lipids biosynthesis (4.73 g L−1) in the medium with glycerol showed R. glutinis var. rubescens LOCKR13. In all investigated strains, oleic acid had the highest proportion of the total fatty acids. Nutritionally beneficial features were demonstrated by R. mucilaginosa, strain ATCC 66034 containing docosapentaenoic acid and reaching a high lipids biosynthesis efficiency (3.24 g L−1). Compared to glucose after incubation of R. aurantiaca, R. glutinis, R. gracilis and R. mucilaginosa strains with glycerol, the higher PUFA content per unit volume of medium was observed.

ACS Style

Iwona Gientka; Marta Gadaszewska; Stanisław Błażejak; Marek Kieliszek; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Anna Kot. Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol. European Food Research and Technology 2016, 243, 275 -286.

AMA Style

Iwona Gientka, Marta Gadaszewska, Stanisław Błażejak, Marek Kieliszek, Anna Bzducha-Wróbel, Lidia Stasiak-Różańska, Anna Kot. Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol. European Food Research and Technology. 2016; 243 (2):275-286.

Chicago/Turabian Style

Iwona Gientka; Marta Gadaszewska; Stanisław Błażejak; Marek Kieliszek; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Anna Kot. 2016. "Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol." European Food Research and Technology 243, no. 2: 275-286.

Journal article
Published: 01 July 2016 in Electronic Journal of Biotechnology
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The exopolysaccharides (EPS) produced by yeast exhibit physico-chemical and rheological properties, which are useful in the production of food and in the cosmetic and pharmaceutical industries as well. The effect was investigated of selected carbon sources on the biosynthesis of EPS by Candida famata and Candida guilliermondii strains originally isolated from kefirs. The biomass yields were dependent on carbon source (sucrose, maltose, lactose, glycerol, sorbitol) and ranged from 4.13 to 7.15 g/L. The highest biomass yield was reported for C. guilliermondii after cultivation on maltose. The maximum specific productivity of EPS during cultivation on maltose was 0.505 and 0.321 for C. guilliermondii and C. famata, respectively. The highest EPS yield was found for C. guilliermondii strain. The EPS produced under these conditions contained 65.4% and 61.5% carbohydrates, respectively. The specific growth rate (μ) of C. famata in medium containing EPS as a sole carbon source was 0.0068 h− 1 and 0.0138 h− 1 for C. guilliermondii strain. The most preferred carbon source in the synthesis of EPS for both Candida strains was maltose, wherein C. guilliermondii strain showed the higher yield of EPS biosynthesis. The carbon source affected the chemical composition of the resulting EPS and the contribution of carbohydrate in the precipitated preparation of polymers was higher during supplementation of maltose as compared to sucrose. It was also found that the EPS can be a source of carbon for the producing strains.

ACS Style

Iwona Gientka; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Aleksandra Agnieszka Bednarska; Stanisław Błażejak. The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources. Electronic Journal of Biotechnology 2016, 22, 31 -37.

AMA Style

Iwona Gientka, Anna Bzducha-Wróbel, Lidia Stasiak-Różańska, Aleksandra Agnieszka Bednarska, Stanisław Błażejak. The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources. Electronic Journal of Biotechnology. 2016; 22 ():31-37.

Chicago/Turabian Style

Iwona Gientka; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Aleksandra Agnieszka Bednarska; Stanisław Błażejak. 2016. "The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources." Electronic Journal of Biotechnology 22, no. : 31-37.

Journal article
Published: 16 November 2015 in Acta Scientiarum Polonorum Technologia Alimentaria
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ACS Style

Iwona Gientka; Stanisław Błażejak; Lidia Stasiak-Różańska; Anna Chlebowska-Śmigiel. Exopolysaccharides from yeast: insight into optimal conditions for biosynthesis, chemical composition and functional properties – review. Acta Scientiarum Polonorum Technologia Alimentaria 2015, 14, 283 -292.

AMA Style

Iwona Gientka, Stanisław Błażejak, Lidia Stasiak-Różańska, Anna Chlebowska-Śmigiel. Exopolysaccharides from yeast: insight into optimal conditions for biosynthesis, chemical composition and functional properties – review. Acta Scientiarum Polonorum Technologia Alimentaria. 2015; 14 (4):283-292.

Chicago/Turabian Style

Iwona Gientka; Stanisław Błażejak; Lidia Stasiak-Różańska; Anna Chlebowska-Śmigiel. 2015. "Exopolysaccharides from yeast: insight into optimal conditions for biosynthesis, chemical composition and functional properties – review." Acta Scientiarum Polonorum Technologia Alimentaria 14, no. 4: 283-292.

Journal article
Published: 01 August 2015 in Food Microbiology
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The cultivation of spices and herbs in parts of the world characterized by warm climate and high humidity provides excellent conditions for the development of microorganisms, including the undesirable ones. The aim of this study was to determine the microbiological quality of spices and herbs available on the Polish market, considering the occurrence of Cronobacter species bacteria. Analyses covered 60 samples of commercial spices and herbs, including 38 samples of dried herbs (basil, bay leaves, thyme, oregano, tarragon, marjoram, dill, parsley, rosemary, lovage) and 16 samples of seasoning blends as well as 6 samples of spices seeds and fruits (pimento, black pepper, coriander). All samples were tested for the total count of aerobic mesophilic bacteria (TAMB) and for the presence of Cronobacter spp. In most of the samples of spices and herbs (60.0%), the TAMB did not exceed 10(4) CFU/g, and the level regarded as unacceptable (>10(6) CFU/g) was not identified in any of the samples. The presence of Cronobacter spp. was demonstrated in 10 (16.7%) samples of the analyzed products, however these were mainly samples of herbs (basil, tarragon, parsley) and one sample of a seasoning blend (Provence herbs). The highest microbiological contamination (TAMB) was found in samples of herbs (oregano, tarragon, basil) and in ready seasoning blends, in 21.1% and 25.0% of which the total count of aerobic mesophiles was in the range of 10(5)-10(6) CFU/g. In all samples of spices seeds and fruits (coriander, black pepper and pimento), the total count of aerobic bacteria reached <10(4) CFU/g. Results achieved in the study indicate good hygienic conditions in the production process of spices and herbs available on the Polish market. The study demonstrated also that dried spices and herbs may be carriers of Cronobacter species bacteria, though their presence in not often detected in products of this type.

ACS Style

M. Garbowska; A. Berthold-Pluta; L. Stasiak-Różańska. Microbiological quality of selected spices and herbs including the presence of Cronobacter spp. Food Microbiology 2015, 49, 1 -5.

AMA Style

M. Garbowska, A. Berthold-Pluta, L. Stasiak-Różańska. Microbiological quality of selected spices and herbs including the presence of Cronobacter spp. Food Microbiology. 2015; 49 ():1-5.

Chicago/Turabian Style

M. Garbowska; A. Berthold-Pluta; L. Stasiak-Różańska. 2015. "Microbiological quality of selected spices and herbs including the presence of Cronobacter spp." Food Microbiology 49, no. : 1-5.

Journal article
Published: 15 December 2014 in Molecules
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Selected methods for yeast cell disruption were evaluated to establish their suitability for cell wall preparation in the process of β-glucan isolation. The effect of different disruption methods on contents of total saccharides, β-glucans and proteins in the produced cell walls preparations was analyzed. The degree of cell wall purification from intracellular components was established on the basis of the ratio of solubilised material. The investigated methods included: cell exposure to hot water (autoclaving), thermally-induced autolysis, homogenization in a bead mill, sonication and their combinations. Experimental systems were prepared in water (pH 5.0 and pH 7.0) and Tris-HCl buffer (pH 8.0). The Saccharomyces cerevisiae yeast cell wall preparations with the highest degree of cytosol component release and purification of β-glucans were produced by 30 min of cell homogenization with zirconium-glass beads (0.5 mm in diameter). This was confirmed by the highest ratio of solubilised material (approx. 64%–67%). The thus-produced preparations contained ca. 60% of total saccharides, 13%–14% of β(1,3)/(1,6)-glucans, and approx. 35% of crude proteins. Similar results were obtained after autolysis coupled with bead milling as well as with sonication, but the time required for these processes was more than 24 h. Homogenization in a bead mill could be valuable for general isolation procedures because allows one to eliminate the different autolytic activity of various yeast strains.

ACS Style

Anna Bzducha-Wróbel; Stanisław Błażejak; Anna Kawarska; Lidia Stasiak-Różańska; Iwona Gientka; Ewa Majewska. Evaluation of the Efficiency of Different Disruption Methods on Yeast Cell Wall Preparation for β-Glucan Isolation. Molecules 2014, 19, 20941 -20961.

AMA Style

Anna Bzducha-Wróbel, Stanisław Błażejak, Anna Kawarska, Lidia Stasiak-Różańska, Iwona Gientka, Ewa Majewska. Evaluation of the Efficiency of Different Disruption Methods on Yeast Cell Wall Preparation for β-Glucan Isolation. Molecules. 2014; 19 (12):20941-20961.

Chicago/Turabian Style

Anna Bzducha-Wróbel; Stanisław Błażejak; Anna Kawarska; Lidia Stasiak-Różańska; Iwona Gientka; Ewa Majewska. 2014. "Evaluation of the Efficiency of Different Disruption Methods on Yeast Cell Wall Preparation for β-Glucan Isolation." Molecules 19, no. 12: 20941-20961.

Applied genetics and molecular biotechnology
Published: 20 September 2014 in Applied Microbiology and Biotechnology
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In a number of previous studies, our group has discovered an alternative pathway for lactose utilization in Lactococcus lactis that, in addition to a sugar-hydrolyzing enzyme with both P-β-glucosidase and P-β-galactosidase activity (BglS), engages chromosomally encoded components of cellobiose-specific PTS (PTS(Cel-Lac)), including PtcA, PtcB, and CelB. In this report, we show that this system undergoes regulation via ClaR, a novel activator protein from the RpiR family of transcriptional regulators. Although RpiR proteins are widely distributed among lactic acid bacteria, their roles have yet to be confirmed by functional assays. Here, we show that ClaR activity depends on intracellular cellobiose-6-phosphate availability, while other sugars such as glucose or galactose have no influence on it. We also show that ClaR is crucial for activation of the bglS and celB expression in the presence of cellobiose, with some limited effects on ptcA and ptcB activation. Among 190 of carbon sources tested, the deletion of claR reduces L. lactis growth only in lactose- and/or cellobiose-containing media, suggesting a narrow specificity of this regulator within the context of sugar metabolism.

ACS Style

Tamara Aleksandrzak-Piekarczyk; Lidia Stasiak-Różańska; Jarosław Cieśla; Jacek Bardowski. ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403. Applied Microbiology and Biotechnology 2014, 99, 337 -47.

AMA Style

Tamara Aleksandrzak-Piekarczyk, Lidia Stasiak-Różańska, Jarosław Cieśla, Jacek Bardowski. ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403. Applied Microbiology and Biotechnology. 2014; 99 (1):337-47.

Chicago/Turabian Style

Tamara Aleksandrzak-Piekarczyk; Lidia Stasiak-Różańska; Jarosław Cieśla; Jacek Bardowski. 2014. "ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403." Applied Microbiology and Biotechnology 99, no. 1: 337-47.

Journal article
Published: 20 May 2014 in European Food Research and Technology
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Bacteria of the species Gluconobacter oxydans are applied in the industrial production of dihydroxyacetone (DHA) via glycerol oxidation. The major problem of this biotransformation involves process inhibition by substrate and/or product. Improper initial concentration of glycerol and increasing DHA concentration may inhibit the metabolic activity of bacterial cells and impede further course of the reaction. An attempt was, therefore, undertaken in this study to determine which concentrations of glycerol (30, 50, 70, 100 g L−1) and DHA (10–100 g L−1) may inhibit the growth of acetic acid bacteria of G. oxydans ATCC 621 species. Cultures of this strain were run in the Bioscreen C MBR apparatus on experimental culture media with various initial concentrations of glycerol and DHA. Analyses were also carried out to examine the impact of pH (5.0, 7.0, 8.0) of glycerol-containing culture media on cell growth of the analyzed strain G. oxydans. None of the applied substrate concentrations was inhibiting cellular divisions of G. oxydans bacteria. The initial glycerol concentrations that enabled rapid cellular divisions reached 50 g L−1 in the medium with pH 5.0 (coefficient of specific growth rate μ = 0.0550) and 70 g L−1 in the medium with pH 7.0 (μ = 0.0556). DHA was shown to inhibit the mitotic activity of G. oxydans bacteria even at low concentrations (20–30 g L−1), whereas at the concentration of 70 g L−1, it made cell divisions impossible. The applied pH values of the culture media did not inhibit the growth of G. oxydans strain.

ACS Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka. Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621. European Food Research and Technology 2014, 239, 453 -461.

AMA Style

Lidia Stasiak-Różańska, Stanisław Błażejak, Iwona Gientka. Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621. European Food Research and Technology. 2014; 239 (3):453-461.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka. 2014. "Effect of glycerol and dihydroxyacetone concentrations in the culture medium on the growth of acetic acid bacteria Gluconobacter oxydans ATCC 621." European Food Research and Technology 239, no. 3: 453-461.

Journal article
Published: 09 October 2012 in European Food Research and Technology
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Dihydroxyacetone (1,3-dihydroxy-2-propanone, DHA) is applied in the food and cosmetic industries as well as in pharmacy and medicine. It is produced as a result of incomplete oxidation of glycerol by acetic acid bacteria Gluconobacter oxydans. This reaction is catalyzed by PQQ-dependent membrane-bound glycerol dehydrogenase. The research developed a method of obtaining DHA by oxidation of a 3 % aqueous solution of glycerol (pH 7.5) at a temperature of 23 °C, with the only reaction biocatalyst being an immobilized cell preparation obtained from G. oxydans cells. After 5 days of the process, DHA concentration in the solution accounted for 27.2 g/L and the reaction efficiency for 94 %. After 4 days of the reaction run in culture media with pH 5.0, at a temperature of 28 °C, free or immobilized cells of G. oxydans produced on average 25 g of DHA/L at the reaction efficiency of 87 %.

ACS Style

Stasiak-Różańska Lidia; Błażejak Stanisław. Production of dihydroxyacetone from an aqueous solution of glycerol in the reaction catalyzed by an immobilized cell preparation of acetic acid bacteria Gluconobacter oxydans ATCC 621. European Food Research and Technology 2012, 235, 1125 -1132.

AMA Style

Stasiak-Różańska Lidia, Błażejak Stanisław. Production of dihydroxyacetone from an aqueous solution of glycerol in the reaction catalyzed by an immobilized cell preparation of acetic acid bacteria Gluconobacter oxydans ATCC 621. European Food Research and Technology. 2012; 235 (6):1125-1132.

Chicago/Turabian Style

Stasiak-Różańska Lidia; Błażejak Stanisław. 2012. "Production of dihydroxyacetone from an aqueous solution of glycerol in the reaction catalyzed by an immobilized cell preparation of acetic acid bacteria Gluconobacter oxydans ATCC 621." European Food Research and Technology 235, no. 6: 1125-1132.

Journal article
Published: 11 January 2012 in Acta Scientiarum Polonorum Technologia Alimentaria
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ACS Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Aleksandra Miklaszewska. Application of immobilized cell preparation obtained from biomass of Gluconacetobacter xylinus bacteria in biotransformation of glycerol to dihydroxyacetone. Acta Scientiarum Polonorum Technologia Alimentaria 2012, 10, 1 .

AMA Style

Lidia Stasiak-Różańska, Stanisław Błażejak, Aleksandra Miklaszewska. Application of immobilized cell preparation obtained from biomass of Gluconacetobacter xylinus bacteria in biotransformation of glycerol to dihydroxyacetone. Acta Scientiarum Polonorum Technologia Alimentaria. 2012; 10 (1):1.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Aleksandra Miklaszewska. 2012. "Application of immobilized cell preparation obtained from biomass of Gluconacetobacter xylinus bacteria in biotransformation of glycerol to dihydroxyacetone." Acta Scientiarum Polonorum Technologia Alimentaria 10, no. 1: 1.