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Dr. Anna Bzducha-Wróbel
Department of Biotechnology and Food Microbiology, Warsaw University of Life Sciences-SGGW, Nowoursynowska Str. 159c, 02-776 Warsaw, Poland

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0 Microbiology
0 Mycology
0 lactic acid bacteria
0 waste valorization
0 Food biotechnology

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Journal article
Published: 17 May 2019 in Food Chemistry
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The effects of 10 and 20 days of fermentation followed by freeze-drying on the vitamin C and fatty acids contents, chemical conversions and overall chemical composition of Jerusalem artichoke were studied. Fermentation between the 10th to 20th days increased content of all saturated fatty acids and two of the four unsaturated fatty acids. The only fatty acid content that decreased was that of C18:1 cis 9 acid, which was suggested to be converted to other fatty acids. The experimental data, which were supported by energetical feasibility, suggested the reaction pathways of the mutual conversions of fatty acids and confirmed the decreased vitamin C content during fermentation. Discriminant modelling of the spectral data successfully distinguished the fresh, 10 days and 20 days fermented samples. The correlation of the spectral and reference data allowed to construct reference models for predicting the content of vitamin C and C18:1 cis 9 fatty acid.

ACS Style

Piotr Koczoń; Tomasz Niemiec; Bartłomiej Jan Bartyzel; Eliza Gruczyńska; Anna Bzducha-Wróbel; Pola Koczoń. Chemical changes that occur in Jerusalem artichoke silage. Food Chemistry 2019, 295, 172 -179.

AMA Style

Piotr Koczoń, Tomasz Niemiec, Bartłomiej Jan Bartyzel, Eliza Gruczyńska, Anna Bzducha-Wróbel, Pola Koczoń. Chemical changes that occur in Jerusalem artichoke silage. Food Chemistry. 2019; 295 ():172-179.

Chicago/Turabian Style

Piotr Koczoń; Tomasz Niemiec; Bartłomiej Jan Bartyzel; Eliza Gruczyńska; Anna Bzducha-Wróbel; Pola Koczoń. 2019. "Chemical changes that occur in Jerusalem artichoke silage." Food Chemistry 295, no. : 172-179.

Journal article
Published: 30 March 2019 in Toxins
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Mycotoxins are harmful contaminants of food and feed worldwide. Feed additives with the abilities to trap mycotoxins are considered substances which regulate toxin transfer from feed to tissue, reducing its absorption in animal digestive tract. Market analysis emphasizes growing interest of feed producers in mycotoxins binders obtained from yeast biomass. The aim of the study was to prescreen cell walls (CW) and β(1,3)/(1,6)-glucan (β-G) preparations isolated from Candida utilis ATCC 9950 cultivated on waste potato juice water with glycerol as adsorbents for aflatoxin B1 (AFB1), zearalenone (ZEN), ochratoxin A (OTA), deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T-2) and fumonisin B1 (FB1). The adsorption was studied in single concentration tests at pH 3.0 and 6.0 in the presence of 1% of the adsorbent and 500 ng/mL of individual toxin. Evaluated CW and β-G preparations had the potential to bind ZEN, OTA and AFB1 rather than DON, NIV, T-2 toxin and FB1. The highest percentage of adsorption (about 83%), adsorption capacity (approx. 41 µg/ g preparation) and distribution coefficient (458.7mL/g) was found for zearalenone when CW preparation was used under acidic conditions. Higher protein content in CW and smaller particles sizes of the formulation could influence more efficient binding of ZEN, OTA, DON and T-2 toxin at appropriate pH compared to purified β-G. Obtained results show the possibility to transform the waste potato juice water into valuable Candida utilis ATCC 9950 preparation with mycotoxins adsorption properties. Further research is important to improve the binding capacity of studied preparations by increasing the active surface of adsorption.

ACS Style

Anna Bzducha-Wróbel; Marcin Bryła; Iwona Gientka; Stanisław Błażejak; Monika Janowicz. Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap. Toxins 2019, 11, 192 .

AMA Style

Anna Bzducha-Wróbel, Marcin Bryła, Iwona Gientka, Stanisław Błażejak, Monika Janowicz. Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap. Toxins. 2019; 11 (4):192.

Chicago/Turabian Style

Anna Bzducha-Wróbel; Marcin Bryła; Iwona Gientka; Stanisław Błażejak; Monika Janowicz. 2019. "Candida utilis ATCC 9950 Cell Walls and β(1,3)/(1,6)-Glucan Preparations Produced Using Agro-Waste as a Mycotoxins Trap." Toxins 11, no. 4: 192.

Original paper
Published: 20 March 2019 in Waste and Biomass Valorization
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β(1,3)/(1,6)-Glucan of yeast origin is a bioactive polysaccharide used in nutrition, healthcare, agriculture and wastewater treatment. Yeast cultivation focused on the biosynthesis of β-glucan with simultaneous utilization of industrial wastes as a culture medium is a new way of obtaining these functional polysaccharides. The aim of current study was to isolate and characterize the purified β(1,3)/(1,6)-glucan preparation from Candida utilis ATTC 9950 biomass propagated in waste deproteinated potato juice water with the addition of glycerol. The production of biomass of tested C. utilis strain was carried on biofermentor scale. The isolation of cell wall β-glucan was tested with two different methods: the alkaline-acid procedure coupled with hot-water extraction (method 1) and isolation based on cell wall autoclaving, lipids extraction and proteolysis (method 2). The isolated fractions were further analyzed with infrared spectroscopy. Obtained preparations contained approx. 82% of β(1,3)/(1,6)-glucan after shortened extraction comparing with procedures described in literature. Infrared spectra of examined preparations demonstrated highly similar pattern to β-glucan standard of Saccharomyces cerevisiae origin. The preparation produced by method 2 showed higher ratio of β(1,3)- to β(1,6)-glucan. The production efficiency for method 2 was 70%, while for the method 1 it was significantly lower, i.e. 49%, indicating substantial material losses during alkaline-acid purification. The C. utilis ATCC 9950 yeast cultivated on waste deproteinated potato juice water supplemented with glycerol is a highly efficient system to obtain purified β-glucan preparation.

ACS Style

Anna Bzducha-Wróbel; Piotr Koczoń; Stanisław Błażejak; Jakub Kozera; Marek Kieliszek. Valorization of Deproteinated Potato Juice Water into β-Glucan Preparation of C. utilis Origin: Comparative Study of Preparations Obtained by Two Isolation Methods. Waste and Biomass Valorization 2019, 11, 3257 -3271.

AMA Style

Anna Bzducha-Wróbel, Piotr Koczoń, Stanisław Błażejak, Jakub Kozera, Marek Kieliszek. Valorization of Deproteinated Potato Juice Water into β-Glucan Preparation of C. utilis Origin: Comparative Study of Preparations Obtained by Two Isolation Methods. Waste and Biomass Valorization. 2019; 11 (7):3257-3271.

Chicago/Turabian Style

Anna Bzducha-Wróbel; Piotr Koczoń; Stanisław Błażejak; Jakub Kozera; Marek Kieliszek. 2019. "Valorization of Deproteinated Potato Juice Water into β-Glucan Preparation of C. utilis Origin: Comparative Study of Preparations Obtained by Two Isolation Methods." Waste and Biomass Valorization 11, no. 7: 3257-3271.

Original article
Published: 07 February 2019 in Molecular Biology Reports
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Selenium exhibits health-promoting properties in humans and animals. Therefore, the development of selenium-enriched dietary supplements has been growing worldwide. However, it may also exhibit toxicity at higher concentrations, causing increased oxidative stress. Different species of yeasts may exhibit different tolerances toward selenium. Therefore, in this study, we aimed to determine the effect of selenium on growth and on the antioxidative system in Candida utilis ATCC 9950 and Saccharomyces cerevisiae ATCC MYA-2200 yeast cells. The results of this study have demonstrated that high doses of selenium causes oxidative stress in yeasts, thereby increasing the process of lipid peroxidation. In addition, we obtained an increased level of GSSG from aqueous solutions of yeast biomass grown with selenium supplementation (40–60 mg/L). Increased levels of selenium in aqueous solutions resulted in an increase in the activity of antioxidant enzymes, including glutathione peroxidase and glutathione reductase. These results should encourage future research on the possibility of a thorough understanding of antioxidant system functioning in yeast cells.

ACS Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel; Anna M. Kot. Effect of selenium on growth and antioxidative system of yeast cells. Molecular Biology Reports 2019, 46, 1797 -1808.

AMA Style

Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M. Kot. Effect of selenium on growth and antioxidative system of yeast cells. Molecular Biology Reports. 2019; 46 (2):1797-1808.

Chicago/Turabian Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel; Anna M. Kot. 2019. "Effect of selenium on growth and antioxidative system of yeast cells." Molecular Biology Reports 46, no. 2: 1797-1808.

Biotechnological products and process engineering
Published: 13 September 2018 in Applied Microbiology and Biotechnology
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New ideas on production of yeast origin β-glucan preparations for industrial application are attracting interest considering market development of that high-value functional polysaccharide. Sellecting an efficient yeast producer and designing culture conditions are a prerequisite for obtaining high yield of β-glucan. The aim of this study was to describe at the first time the influence of the mode of cultivation (shake-flasks and batch fermentation) and time of culture on characteristic and yield of biomass and β(1,3)/(1,6)-glucan preparations of Candida utilis ATCC 9950 after cultivation in medium based on waste potato juice water supplemented with 10% of glycerol. After shake-flask culture, the biomass was characterized by higher protein content (app. 26.5%) compared to 19% after batch fermentation while the cultivation on a biofermentor scale promoted polysaccharides biosynthesis. The highest output of purified β(1,3)/(1,6)-glucan preparation (5.3 gd.w./L), containing app. 85% of that polysaccharide, was found after 48 h cultivation in biofermentor. Batch fermentation promoted biosynthesis of alkali-insoluble β(1,3)/(1,6)-glucan fraction, decreasing the content of β(1,6)-glucan. The yield of β(1,3)/(1,6)-glucan synthesis was 0.063 (g/g glycerol), while the productivity of that polysaccharide reached 0.094 (g/L/h). Longer batch fermentation (72 h) resulted in reduction of production efficiency of β-glucan preparation under studied conditions. The results of the study provide a new efficient biotechnological solution to produce high-value β-glucan preparations of C. utilis origin based on valorization of agro-waste potato juice water with glycerol.

ACS Style

Anna Bzducha-Wróbel; Katarzyna Pobiega; Stanisław Błażejak; Marek Kieliszek. The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield. Applied Microbiology and Biotechnology 2018, 102, 9131 -9145.

AMA Style

Anna Bzducha-Wróbel, Katarzyna Pobiega, Stanisław Błażejak, Marek Kieliszek. The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield. Applied Microbiology and Biotechnology. 2018; 102 (21):9131-9145.

Chicago/Turabian Style

Anna Bzducha-Wróbel; Katarzyna Pobiega; Stanisław Błażejak; Marek Kieliszek. 2018. "The scale-up cultivation of Candida utilis in waste potato juice water with glycerol affects biomass and β(1,3)/(1,6)-glucan characteristic and yield." Applied Microbiology and Biotechnology 102, no. 21: 9131-9145.

Journal article
Published: 01 September 2018 in Journal of Biotechnology
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Changes in cell wall structure of four strains of Sacccharomyces cerevisiae species (brewer’s, baker’s and probiotic yeast) after culturing on deproteinated potato juice water (DPJW) with diverse addition of glycerol and different pH were investigated. It allowed to select conditions intensifying biosynthesis of β(1,3)/(1,6)-glucan and mannoproteins of cell walls of tested strains. Yeast cell wall structural polysaccharides show biological activity and technological usability in food industry but also decide about therapeutic properties of yeast biomass. The highest increase in the thickness of walls (by about 100%) and β-glucan layer (by about 120%) was stated after cultivation of S. cerevisiae R9 brewer’s yeast in DPJW supplemented with 5 and 10% (w/v) of glycerol and pH 7.0 while S. cerevisiae var. boulardi PAN yeast synthesized by ab. 70% thicker β-glucan layer when the pH of growth medium was equal to 5.0. The cells of brewer’s yeast (S. cerevisiae R9), probiotic (S. cerevisiae CNCM 1-745) and baker’s (S. cerevisiae 102) intensified the ratio of mannoproteins in the structure of cell walls cultivated in mediums supplemented with above 15% of glycerol what point out the protective action of glycoprotein’s under osmotic stress conditions. The study confirms at the first time the possibility of using agro-industrial waste in biosynthesis of functional polysaccharides of S. cerevisiae cell wall. It could be an new advantage in production of yeast biomass with therapeutic properties or β-glucan preparation as a novel food ingredient.

ACS Style

Anna Bzducha-Wróbel; Stanisław Błażejak; Marek Kieliszek; Katarzyna Pobiega; Katarzyna Falana; Monika Janowicz. Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides. Journal of Biotechnology 2018, 281, 1 -10.

AMA Style

Anna Bzducha-Wróbel, Stanisław Błażejak, Marek Kieliszek, Katarzyna Pobiega, Katarzyna Falana, Monika Janowicz. Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides. Journal of Biotechnology. 2018; 281 ():1-10.

Chicago/Turabian Style

Anna Bzducha-Wróbel; Stanisław Błażejak; Marek Kieliszek; Katarzyna Pobiega; Katarzyna Falana; Monika Janowicz. 2018. "Modification of the cell wall structure of Saccharomyces cerevisiae strains during cultivation on waste potato juice water and glycerol towards biosynthesis of functional polysaccharides." Journal of Biotechnology 281, no. : 1-10.

Journal article
Published: 01 September 2018 in Zeszyty Problemowe Postępów Nauk Rolniczych
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ACS Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Anna Bzducha-Wróbel. Effect of the additive of pyrroloquinoline quinone on waste glycerol bioconversion to dihydroxyacetone. Zeszyty Problemowe Postępów Nauk Rolniczych 2018, 59 -67.

AMA Style

Lidia Stasiak-Różańska, Anna Berthold-Pluta, Anna Bzducha-Wróbel. Effect of the additive of pyrroloquinoline quinone on waste glycerol bioconversion to dihydroxyacetone. Zeszyty Problemowe Postępów Nauk Rolniczych. 2018; (594):59-67.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Anna Berthold-Pluta; Anna Bzducha-Wróbel. 2018. "Effect of the additive of pyrroloquinoline quinone on waste glycerol bioconversion to dihydroxyacetone." Zeszyty Problemowe Postępów Nauk Rolniczych , no. 594: 59-67.

Correction
Published: 01 June 2018 in Biological Trace Element Research
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The authors forgot to include the following information in Materials and Methods.

ACS Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel; Anna M. Kot. Correction to: Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells. Biological Trace Element Research 2018, 187, 328 -328.

AMA Style

Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Anna M. Kot. Correction to: Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells. Biological Trace Element Research. 2018; 187 (1):328-328.

Chicago/Turabian Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel; Anna M. Kot. 2018. "Correction to: Effect of Selenium on Lipid and Amino Acid Metabolism in Yeast Cells." Biological Trace Element Research 187, no. 1: 328-328.

Original paper
Published: 25 November 2017 in Antonie van Leeuwenhoek
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The purpose of this study was to determine the potential for biosynthesis of propionic acid and vitamin B12 by Propionibacterium freudenreichii T82 in a medium containing various sources of carbon (glucose, fructose, and saccharose). These sugars are present in apple pomaces, which are the waste from the production of apple juice. Using statistical analysis design of experiments (DoE), the results allowed us to determine which sugars (carbon sources) exert the most beneficial influence on the biosynthesis of propionic acid and cobalamin. The highest production of propionic acid by the tested bacterial strain was obtained in a medium in which glucose accounted for at least 50% of the available carbon sources. Depending on the culture medium, the concentration of this metabolite ranged from 23 to 40 g/L. P. freudenreichii T82 produced the smallest amount of acid in medium in which the dominant nutrient source was saccharose. The results obtained indicated an inverse relationship between the amount of acid produced by the bacteria and vitamin B12 biosynthesis. Because of the high efficiency of propionic acid biosynthesis by P. freudenreichii T82, the prospect of using this strain to obtain propionate with the simultaneous disposal of waste materials (such as apple pomaces) which contain glucose and/or fructose is very promising.

ACS Style

Kamil Piwowarek; Edyta Lipińska; Elżbieta Hać-Szymańczuk; Anna Bzducha-Wróbel; Alicja Synowiec. Research on the ability of propionic acid and vitamin B12 biosynthesis by Propionibacterium freudenreichii strain T82. Antonie van Leeuwenhoek 2017, 111, 921 -932.

AMA Style

Kamil Piwowarek, Edyta Lipińska, Elżbieta Hać-Szymańczuk, Anna Bzducha-Wróbel, Alicja Synowiec. Research on the ability of propionic acid and vitamin B12 biosynthesis by Propionibacterium freudenreichii strain T82. Antonie van Leeuwenhoek. 2017; 111 (6):921-932.

Chicago/Turabian Style

Kamil Piwowarek; Edyta Lipińska; Elżbieta Hać-Szymańczuk; Anna Bzducha-Wróbel; Alicja Synowiec. 2017. "Research on the ability of propionic acid and vitamin B12 biosynthesis by Propionibacterium freudenreichii strain T82." Antonie van Leeuwenhoek 111, no. 6: 921-932.

Review
Published: 01 September 2017 in Fungal Biology Reviews
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ACS Style

Marek Kieliszek; Anna Kot; Anna Bzducha-Wróbel; Stanisław Błażejak; Iwona Gientka; Agnieszka Kurcz. Biotechnological use of Candida yeasts in the food industry: A review. Fungal Biology Reviews 2017, 31, 185 -198.

AMA Style

Marek Kieliszek, Anna Kot, Anna Bzducha-Wróbel, Stanisław Błażejak, Iwona Gientka, Agnieszka Kurcz. Biotechnological use of Candida yeasts in the food industry: A review. Fungal Biology Reviews. 2017; 31 (4):185-198.

Chicago/Turabian Style

Marek Kieliszek; Anna Kot; Anna Bzducha-Wróbel; Stanisław Błażejak; Iwona Gientka; Agnieszka Kurcz. 2017. "Biotechnological use of Candida yeasts in the food industry: A review." Fungal Biology Reviews 31, no. 4: 185-198.

Journal article
Published: 01 May 2017 in Electronic Journal of Biotechnology
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Background: Depletion of petroleum resources has enforced the search for alternative sources of renewable energy. Introduction of biofuels into the market was expected to become a solution to this disadvantageous situation. Attempts to cover fuel demand have, however, caused another severe problem—the waste glycerol generated during biodiesel production at a concentration of approximately 10% w/w. This, in turn, prompted a global search for effective methods of valorization of the waste fraction of glycerol. Results: Utilization of the waste fraction at 48 h with an initial glycerol concentration of 30 g·L-1 and proceeding with 62% efficiency enabled the production of 9 g·L-1 dihydroxyacetone at 50% substrate consumption. The re-use of the immobilized biocatalyst resulted in a similar concentration of dihydroxyacetone (8.7 g·L-1) in two-fold shorter time, with an efficiency of 85% and lower substrate consumption (35%). Conclusions: The method proposed in this work is based on the conversion of waste glycerol to dihydroxyacetone in a reaction catalyzed by immobilized Gluconobacter oxydans cell extract with glycerol dehydrogenase activity, and it could be an effective way to convert waste glycerol into a valuable product

ACS Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel; Edyta Lipińska. Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract. Electronic Journal of Biotechnology 2017, 27, 44 -48.

AMA Style

Lidia Stasiak-Różańska, Stanisław Błażejak, Iwona Gientka, Anna Bzducha-Wróbel, Edyta Lipińska. Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract. Electronic Journal of Biotechnology. 2017; 27 ():44-48.

Chicago/Turabian Style

Lidia Stasiak-Różańska; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel; Edyta Lipińska. 2017. "Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract." Electronic Journal of Biotechnology 27, no. : 44-48.

Journal article
Published: 01 May 2017 in Electronic Journal of Biotechnology
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Background: Rhodotorula glutinis is capable of synthesizing numerous valuable metabolites with extensive potential industrial usage. This paper reports the effect of initial culture medium pH on growth and protein, lipid, and carotenoid biosynthesis by R. glutinis. Results: The highest biomass yield was obtained in media with pH 4.0–7.0, and the value after 72 h was 17.2–19.4 gd.w./L. An initial pH of the medium in the range of 4.0–7.0 has no significant effect on the protein (38.5–41.3 g/100 gd.w.), lipid (10.2–12.7 g/100 gd.w.), or carotenoid (191.7–202.9 μg/gd.w.) content in the biomass or on the profile of synthesized fatty acids and carotenoids. The whole pool of fatty acids was dominated by oleic (48.1–53.4%), linoleic (21.4–25.1%), and palmitic acids (13.0–15.8%). In these conditions, the yeast mainly synthesized torulene (43.5–47.7%) and β-carotene (34.7–38.6%), whereas the contribution of torularhodin was only 12.1–16.8%. Cultivation in medium with initial pH 3.0 resulted in a reduction in growth (13.0 gd.w./L) and total carotenoid (115.8 μg/gd.w.), linoleic acid (11.5%), and torularhodin (4.5%) biosynthesis. Conclusion: The different values of initial pH of the culture medium with glycerol and deproteinized potato wastewater had a significant effect on the growth and protein, lipid, and carotenoid biosynthesis by R. glutinis

ACS Style

Anna Kot; Stanisław Błażejak; Agnieszka Kurcz; Joanna Bryś; Iwona Gientka; Anna Bzducha-Wróbel; Magdalena Maliszewska; Lidia Reczek. Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis. Electronic Journal of Biotechnology 2017, 27, 25 -31.

AMA Style

Anna Kot, Stanisław Błażejak, Agnieszka Kurcz, Joanna Bryś, Iwona Gientka, Anna Bzducha-Wróbel, Magdalena Maliszewska, Lidia Reczek. Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis. Electronic Journal of Biotechnology. 2017; 27 ():25-31.

Chicago/Turabian Style

Anna Kot; Stanisław Błażejak; Agnieszka Kurcz; Joanna Bryś; Iwona Gientka; Anna Bzducha-Wróbel; Magdalena Maliszewska; Lidia Reczek. 2017. "Effect of initial pH of medium with potato wastewater and glycerol on protein, lipid and carotenoid biosynthesis by Rhodotorula glutinis." Electronic Journal of Biotechnology 27, no. : 25-31.

Journal article
Published: 21 December 2016 in Journal of Food Protection
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The antimold activity of lactic acid bacteria (LAB) is used in food biopreservation. The aim of this study was to evaluate the effect of magnesium acetate added to de Man Rogosa Sharpe (MRS) medium on the antimold activity of three LAB strains (Lactobacillus plantarum, Lactobacillus brevis, and Lactobacillus fermentum) against molds contaminating food (Aspergillus oryzae, Aspergillus niger, Penicillium chrysogenum, Fusarium avenaceum, and Rhizopus arrhizus) and their ability to produce organic acids (acetic acid, lactic acid, and phenyllactic acid). The antimold activity of LAB strains was evaluated using the overlay method, and the concentration of the organic acids was determined with the gas chromatography technique. Changes in viable cell counts and the pH of LAB culture also were monitored over a 48-h period. The results show that the growth inhibition of all the molds (except R. arrhizus) was higher in LAB strain cultures on MRS with magnesium acetate agar than on MRS agar, and inhibition increased over the 48 h. Magnesium acetate added to MRS broth stimulated the production of acetic acid by all LAB strains in the first 8 h and slightly stimulated the production of lactic acid by L. plantarum during the first 24 h. No adverse effect of magnesium acetate on growth of LAB strains was noted. The results confirm that magnesium acetate enhances the antimold activity of LAB strains.

ACS Style

Katarzyna Kycia; Anna Bzducha-Wróbel; Karolina Kraśniewska; Anna Chlebowska-Śmigiel; Małgorzata Gniewosz. Effect of Magnesium Acetate on the Antimold Activity of Lactobacillus. Journal of Food Protection 2016, 80, 96 -103.

AMA Style

Katarzyna Kycia, Anna Bzducha-Wróbel, Karolina Kraśniewska, Anna Chlebowska-Śmigiel, Małgorzata Gniewosz. Effect of Magnesium Acetate on the Antimold Activity of Lactobacillus. Journal of Food Protection. 2016; 80 (1):96-103.

Chicago/Turabian Style

Katarzyna Kycia; Anna Bzducha-Wróbel; Karolina Kraśniewska; Anna Chlebowska-Śmigiel; Małgorzata Gniewosz. 2016. "Effect of Magnesium Acetate on the Antimold Activity of Lactobacillus." Journal of Food Protection 80, no. 1: 96-103.

Original paper
Published: 21 November 2016 in Waste and Biomass Valorization
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The aim of this study was to determine the use of glycerol as a source of carbon and the use of deproteinized potato wastewater as a source of nitrogen and mineral components for the production of biomass by fodder yeast Candida utilis. The yeast strain Candida utilis ATCC 9950 was used in this study. Experimental media contained potato wastewater and glycerol at concentrations of 5, 10, 15, 20, and 25% (w/v). Control medium was YPD and potato wastewater. During the 72 h of cultivation (200 rpm, 28 °C), the optical density (spectrophotometric method), biomass yield (weight method), glycerol (chemical method), and protein (Kjeldahl’s method) concentration in the experimental media, protein content in the biomass (Kjeldahl’s method) and chemical-oxygen demand index (dichromate method) in selected culture media were determined. Candida utilis was able to grow in the medium composed of potato wastewater and glycerol. Addition of 5% of glycerol gave a biomass yield above 30 gd.w./L and the efficiency of protein biosynthesis was 12.2 g/L. The results showed that the cultivation C. utilis in this medium caused a significant reduction of glycerol (83%), nitrogen present in potato wastewater (51%), and a high degree of COD index reduction (91%). The study demonstrated that potato wastewater supplemented with glycerol may be successfully used as a carbon source in the production of fodder yeast C. utilis ATCC 9950 biomass. In addition, the results show that these two troublesome industrial wastes could be efficiently used in such processes.

ACS Style

Agnieszka Kurcz; Stanisław Błażejak; Anna M. Kot; Anna Bzducha-Wróbel; Marek Kieliszek. Application of Industrial Wastes for the Production of Microbial Single-Cell Protein by Fodder Yeast Candida utilis. Waste and Biomass Valorization 2016, 9, 57 -64.

AMA Style

Agnieszka Kurcz, Stanisław Błażejak, Anna M. Kot, Anna Bzducha-Wróbel, Marek Kieliszek. Application of Industrial Wastes for the Production of Microbial Single-Cell Protein by Fodder Yeast Candida utilis. Waste and Biomass Valorization. 2016; 9 (1):57-64.

Chicago/Turabian Style

Agnieszka Kurcz; Stanisław Błażejak; Anna M. Kot; Anna Bzducha-Wróbel; Marek Kieliszek. 2016. "Application of Industrial Wastes for the Production of Microbial Single-Cell Protein by Fodder Yeast Candida utilis." Waste and Biomass Valorization 9, no. 1: 57-64.

Journal article
Published: 14 July 2016 in European Food Research and Technology
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Single cell oils (SCO) can be a source of nutritionally valuable fatty acids and raw material in biodiesel production. Among the oleaginous yeasts, those which additionally generate carotenoid pigments are beneficially distinguished. Rhodotorula and Sporobolomyces strains were screened for the ability to grow and produce intracellular lipids in medium containing pure glycerol. In this paper, we verified that glycerol is unfavorable carbon source for a strain of S. salmonicolor and a good source of carbon for the growth and increases lipids content in the biomass of examined Rhodotorula yeast compared to glucose. The best volumetric efficiency of lipids biosynthesis (4.73 g L−1) in the medium with glycerol showed R. glutinis var. rubescens LOCKR13. In all investigated strains, oleic acid had the highest proportion of the total fatty acids. Nutritionally beneficial features were demonstrated by R. mucilaginosa, strain ATCC 66034 containing docosapentaenoic acid and reaching a high lipids biosynthesis efficiency (3.24 g L−1). Compared to glucose after incubation of R. aurantiaca, R. glutinis, R. gracilis and R. mucilaginosa strains with glycerol, the higher PUFA content per unit volume of medium was observed.

ACS Style

Iwona Gientka; Marta Gadaszewska; Stanisław Błażejak; Marek Kieliszek; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Anna Kot. Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol. European Food Research and Technology 2016, 243, 275 -286.

AMA Style

Iwona Gientka, Marta Gadaszewska, Stanisław Błażejak, Marek Kieliszek, Anna Bzducha-Wróbel, Lidia Stasiak-Różańska, Anna Kot. Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol. European Food Research and Technology. 2016; 243 (2):275-286.

Chicago/Turabian Style

Iwona Gientka; Marta Gadaszewska; Stanisław Błażejak; Marek Kieliszek; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Anna Kot. 2016. "Evaluation of lipid biosynthesis ability by Rhodotorula and Sporobolomyces strains in medium with glycerol." European Food Research and Technology 243, no. 2: 275-286.

Journal article
Published: 01 July 2016 in Electronic Journal of Biotechnology
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The exopolysaccharides (EPS) produced by yeast exhibit physico-chemical and rheological properties, which are useful in the production of food and in the cosmetic and pharmaceutical industries as well. The effect was investigated of selected carbon sources on the biosynthesis of EPS by Candida famata and Candida guilliermondii strains originally isolated from kefirs. The biomass yields were dependent on carbon source (sucrose, maltose, lactose, glycerol, sorbitol) and ranged from 4.13 to 7.15 g/L. The highest biomass yield was reported for C. guilliermondii after cultivation on maltose. The maximum specific productivity of EPS during cultivation on maltose was 0.505 and 0.321 for C. guilliermondii and C. famata, respectively. The highest EPS yield was found for C. guilliermondii strain. The EPS produced under these conditions contained 65.4% and 61.5% carbohydrates, respectively. The specific growth rate (μ) of C. famata in medium containing EPS as a sole carbon source was 0.0068 h− 1 and 0.0138 h− 1 for C. guilliermondii strain. The most preferred carbon source in the synthesis of EPS for both Candida strains was maltose, wherein C. guilliermondii strain showed the higher yield of EPS biosynthesis. The carbon source affected the chemical composition of the resulting EPS and the contribution of carbohydrate in the precipitated preparation of polymers was higher during supplementation of maltose as compared to sucrose. It was also found that the EPS can be a source of carbon for the producing strains.

ACS Style

Iwona Gientka; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Aleksandra Agnieszka Bednarska; Stanisław Błażejak. The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources. Electronic Journal of Biotechnology 2016, 22, 31 -37.

AMA Style

Iwona Gientka, Anna Bzducha-Wróbel, Lidia Stasiak-Różańska, Aleksandra Agnieszka Bednarska, Stanisław Błażejak. The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources. Electronic Journal of Biotechnology. 2016; 22 ():31-37.

Chicago/Turabian Style

Iwona Gientka; Anna Bzducha-Wróbel; Lidia Stasiak-Różańska; Aleksandra Agnieszka Bednarska; Stanisław Błażejak. 2016. "The exopolysaccharides biosynthesis by Candida yeast depends on carbon sources." Electronic Journal of Biotechnology 22, no. : 31-37.

Article
Published: 14 July 2015 in Biological Trace Element Research
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This paper presents the results of microscopic examinations of the yeast cells cultured in yeast extract–peptone–dextrose (YPD) media supplemented with sodium selenite(IV). The analysis of the morphological changes in yeast cells aimed to determine whether the selected selenium doses and culturing time may affect this element accumulation in yeast cell structures in a form of inorganic or organic compounds, as a result of detoxification processes. The range of characteristic morphological changes in yeasts cultivated in experimental media with sodium selenite(IV) was observed, including cell shrinkage and cytoplasm thickening of the changes within vacuole structure. The processes of vacuole disintegration were observed in aging yeast cells in culturing medium, which may indicate the presence of so-called ghost cells lacking intracellular organelles The changes occurring in the morphology of yeasts cultured in media supplemented with sodium selenite were typical for stationary phase of yeast growth. From detailed microscopic observations, larger surface area of the cell (6.03 μm2) and yeast vacuole (2.17 μm2) were noticed after 24-h culturing in the medium with selenium of 20 mg Se4+/L. The coefficient of shape of the yeast cells cultured in media enriched with sodium selenite as well as in the control YPD medium ranged from 1.02 to 1.22. Elongation of cultivation time (up to 48 and 72 h) in the media supplemented with sodium selenite caused a reduction in the surface area of the yeast cell and vacuole due to detoxification processes.

ACS Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel; Agnieszka Kurcz. Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells. Biological Trace Element Research 2015, 169, 387 -393.

AMA Style

Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel, Agnieszka Kurcz. Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells. Biological Trace Element Research. 2015; 169 (2):387-393.

Chicago/Turabian Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel; Agnieszka Kurcz. 2015. "Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells." Biological Trace Element Research 169, no. 2: 387-393.

Research article
Published: 21 June 2015 in Oxidative Medicine and Cellular Longevity
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Selenium is an essential trace element for human health and it has been recognized as a component of several selenoproteins with crucial biological functions. It has been identified as a component of active centers of many enzymes, as well as integral part of biologically active complexes. The aim of the study was to evaluate the protein content and amino acid profile of the protein of fodder yeastCandida utilisATCC 9950 cultured in media control and experimental enriched selenium. Protein analysis was performed using SDS-PAGE method consisting of polyacrylamide gel electrophoresis in the presence of SDS. The highest contents of soluble protein (49,5 mg/g) were found in yeast cells after 24-hour culture conducted in control (YPD) medium. In the presence of selenium there were determined small amounts of protein content. With increasing time of yeast culture (to 72 hours) the control and experimental media were reported to reduce soluble protein content. In electropherogram proteins from control cultures was observed the presence of 10 protein fractions, but in all the experimental cultures (containing 20, 30, and 40 mg/L selenium) of 14 protein fractions. On the basis of the molecular weights of proteins, it can be concluded that they were among others: selenoprotein 15 kDa and selenoprotein 18 kDa.

ACS Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel. Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast CellsCandida utilisATCC 9950. Oxidative Medicine and Cellular Longevity 2015, 2015, 1 -6.

AMA Style

Marek Kieliszek, Stanisław Błażejak, Anna Bzducha-Wróbel. Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast CellsCandida utilisATCC 9950. Oxidative Medicine and Cellular Longevity. 2015; 2015 ():1-6.

Chicago/Turabian Style

Marek Kieliszek; Stanisław Błażejak; Anna Bzducha-Wróbel. 2015. "Influence of Selenium Content in the Culture Medium on Protein Profile of Yeast CellsCandida utilisATCC 9950." Oxidative Medicine and Cellular Longevity 2015, no. : 1-6.

Mini review
Published: 24 May 2015 in Applied Microbiology and Biotechnology
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This paper examines the process of selenium bioaccumulation and selenium metabolism in yeast cells. Yeast cells can bind elements in ionic from the environment and permanently integrate them into their cellular structure. Up to now, Saccharomyces cerevisiae, Candida utilis, and Yarrowia lipolytica yeasts have been used primarily in biotechnological studies to evaluate binding of minerals. Yeast cells are able to bind selenium in the form of both organic and inorganic compounds. The process of bioaccumulation of selenium by microorganisms occurs through two mechanisms: extracellular binding by ligands of membrane assembly and intracellular accumulation associated with the transport of ions across the cytoplasmic membrane into the cell interior. During intracellular metabolism of selenium, oxidation, reduction, methylation, and selenoprotein synthesis processes are involved, as exemplified by detoxification processes that allow yeasts to survive under culture conditions involving the elevated selenium concentrations which were observed. Selenium yeasts represent probably the best absorbed form of this element. In turn, in terms of wide application, the inclusion of yeast with accumulated selenium may aid in lessening selenium deficiency in a diet.

ACS Style

Marek Kieliszek; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel. Accumulation and metabolism of selenium by yeast cells. Applied Microbiology and Biotechnology 2015, 99, 5373 -5382.

AMA Style

Marek Kieliszek, Stanisław Błażejak, Iwona Gientka, Anna Bzducha-Wróbel. Accumulation and metabolism of selenium by yeast cells. Applied Microbiology and Biotechnology. 2015; 99 (13):5373-5382.

Chicago/Turabian Style

Marek Kieliszek; Stanisław Błażejak; Iwona Gientka; Anna Bzducha-Wróbel. 2015. "Accumulation and metabolism of selenium by yeast cells." Applied Microbiology and Biotechnology 99, no. 13: 5373-5382.

Journal article
Published: 04 April 2015 in European Food Research and Technology
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In the original publication, the description of part figures in figure caption 2 contains some errors. The corrected caption and the Fig. 2 are given on the next page.Fig. 2

ACS Style

Anna Bzducha-Wróbel; Stanisław Błażejak; Magdalena Molenda; Lidia Reczek. Erratum to: Biosynthesis of β(1,3)/(1,6)-glucans of cell wall of the yeast Candida utilis ATCC 9950 strains in the culture media supplemented with deproteinated potato juice water and glycerol. European Food Research and Technology 2015, 240, 1281 -1282.

AMA Style

Anna Bzducha-Wróbel, Stanisław Błażejak, Magdalena Molenda, Lidia Reczek. Erratum to: Biosynthesis of β(1,3)/(1,6)-glucans of cell wall of the yeast Candida utilis ATCC 9950 strains in the culture media supplemented with deproteinated potato juice water and glycerol. European Food Research and Technology. 2015; 240 (6):1281-1282.

Chicago/Turabian Style

Anna Bzducha-Wróbel; Stanisław Błażejak; Magdalena Molenda; Lidia Reczek. 2015. "Erratum to: Biosynthesis of β(1,3)/(1,6)-glucans of cell wall of the yeast Candida utilis ATCC 9950 strains in the culture media supplemented with deproteinated potato juice water and glycerol." European Food Research and Technology 240, no. 6: 1281-1282.