One important paradigm in host-parasite evolutionary biology is the ability of parasites to manipulate the phenotype of their hosts to facilitate transmission. In this paper, I examine whether the protozoan parasite Trypanosoma cruzi modifies the developmental time, body size, and survival of its vector, the bloodsucking insect Mepraia spinolai (Hemiptera; Reduviidae). M. spinolai nymphs were experimentally infected when fed on T. cruzi–infected mice (infected group) or kept uninfected when fed on healthy mice (control group). T. cruzi–infected insects showed a retarded developmental time and reduced survival compared with uninfected individuals. The impact of the parasite on the vector was age-dependent as the last three insect molts were the most affected stages. The presence of T. cruzi decreased significantly the weight of male and female insects in the three last stages. When insect sex was taken into account, infected female bugs took longer than infected males to develop into the adult stage, which implies that the impact of T. cruzi is sex-dependent. Results from this study indicate that T. cruzi has a strong impact on life history traits of M. spinolai and provide strong evidence of age- and sex-dependent parasite-induced phenotype modification for insect vectors. The implications of this study along with previously reported feeding behavioral alterations in this insect vector-parasite system suggest that T. cruzi–induced modifications could translate into an enhanced transmission to definitive mammal hosts.

Molecular evidence showed 46.2% of Trypanosoma cruzi infection in Mepraia spinolai insects from North-Central Chile, which is significantly higher than previous reports of up to 26% by microscopic observation. Our results show similar infection levels among nymphal stages, ranging from 38.3 to 54.1%, indicating that younger nymphs could be as important as older ones in parasite transmission. A cautionary note must be stressed to indicate the potential role of M. spinolai in transmitting T. cruzi in country areas due to the high infection level detected by molecular analysis.

Background: Mepraia gajardoiandMepraia spinolaiare endemic triatomine vector species ofTrypanosoma cruzi, a parasite that causes Chagas disease. These vectors inhabit arid, semiarid and Mediterranean areas of Chile.Mepraia gajardoioccurs from 18° to 25°S, andM. spinolaifrom 26° to 34°S. Even though both species are involved inT. cruzitransmission in the Pacific side of the Southern Cone of South America, no study has modelled their distributions at a regional scale. Therefore, the aim of this study is to estimate the potential geographical distribution ofM. spinolaiandM. gajardoiunder current and future climate scenarios.Methods: We used the Maxent algorithm to model the ecological niche ofM. spinolaiandM. gajardoi, estimating their potential distributions from current climate information and projecting their distributions to future climatic conditions under representative concentration pathways (RCP) 2.6, 4.5, 6.0 and 8.5 scenarios. Future predictions of suitability were constructed considering both higher and lower public health risk situations.Results: The current potential distributions of both species were broader than their known ranges. For both species, climate change projections for 2070 in RCP 2.6, 4.5, 6.0 and 8.5 scenarios showed different results depending on the methodology used. The higher risk situation showed new suitable areas, but the lower risk situation modelled a net reduction in the future potential distribution areas ofM. spinolaiandM. gajardoi.Conclusions: The suitable areas for both species may be greater than currently known, generating new challenges in terms of vector control and prevention. Under future climate conditions, these species could modify their potential geographical range. Preventive measures to avoid accidental human vectorial transmission by wild vectors ofT. cruzibecome critical considering the uncertainty of future suitable areas projected in this study.