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Bacterial extracellular polymeric substances (EPSs) present diverse properties of biotechnological interest, such as surface modification, metal adsorption and hydrophobic substances solubilization through surface tension reduction. Thus, there is a growing demand for new producing strains and structurally variable biomolecules with different properties. One approach for scanning this biodiversity consists of exploring environments under selective pressures. The aim of this study was to evaluate the composition of culturable heterotrophic bacterial communities from five different sites from a copper mine in the Amazon biome by an enrichment technique to obtain metal resistant bacteria (lead, arsenic, cadmium, copper and zinc) capable of producing EPSs. The bacterial densities at the sites varied from 2.42 × 103 to 1.34 × 108 NMP mL−1 and the 77 bacterial isolates obtained were classified in four divisions, β-Proteobacteria (16.88%), γ-Proteobacteria (7.29%), Firmicutes (61%) and Actinobacteria (12.98%). Bacillus, Alcaligenes, and Lysinibacillus were the most dominant among the 16 observed genera, but the relative frequency of each varied according to the sample and the metal used in the enrichment culture. 58% of the bacterial strains (45) could produce EPSs. From these, 33 strains showed emulsifying activity (E24), and 9 of them reached values higher than 49%. Only Actinomyces viscosus E3.Pb5 and Bacillus subtilis group E3.As2 reduced the medium surface tension to values lower than 35 mN m−1. It was possible to confirm the high presence of bacteria capable of producing EPSs with tensoactive properties in Amazon copper mines and the evolutionary pressure exerted by the heavy metals during enrichment. These molecules can be tested as an alternative for use in processes that involve the removal of metals, such as the bioremediation of contaminated environments.
Vitor Sousa Domingues; Andrea De Souza Monteiro; Aline Daniela Lopes Júlio; Ana Luiza Lemos Queiroz; Vera Lúcia Dos Santos. Diversity of Metal-Resistant and Tensoactive-Producing Culturable Heterotrophic Bacteria Isolated from a Copper Mine in Brazilian Amazonia. Scientific Reports 2020, 10, 1 -12.
AMA StyleVitor Sousa Domingues, Andrea De Souza Monteiro, Aline Daniela Lopes Júlio, Ana Luiza Lemos Queiroz, Vera Lúcia Dos Santos. Diversity of Metal-Resistant and Tensoactive-Producing Culturable Heterotrophic Bacteria Isolated from a Copper Mine in Brazilian Amazonia. Scientific Reports. 2020; 10 (1):1-12.
Chicago/Turabian StyleVitor Sousa Domingues; Andrea De Souza Monteiro; Aline Daniela Lopes Júlio; Ana Luiza Lemos Queiroz; Vera Lúcia Dos Santos. 2020. "Diversity of Metal-Resistant and Tensoactive-Producing Culturable Heterotrophic Bacteria Isolated from a Copper Mine in Brazilian Amazonia." Scientific Reports 10, no. 1: 1-12.
Candida yeasts are generally found in the vaginal microbiota; however, disruption of the balance maintained by host factors and microorganisms results in vulvovaginal candidiasis (VVC). This study evaluated the antagonistic activity of vaginal Lactobacillus spp. on Candida albicans to verify whether active compounds of Lactobacillus spp. had antifungal and antivirulence activity. The antagonism assay showed that 15 out of 20 Lactobacillus strains had an inhibitory effect on C. albicans. Biosurfactants displayed surface-tension-reducing activity, with the best value obtained for Lactobacillus gasseri 1. Lactobacillus rhamnosus ATCC 9595, Lactobacillus acidophilus ATCC 4356, and Lactobacillus paracasei 11 produced biosurfactants that decreased C. albicans adhesion and disrupted biofilm formation. The best results were obtained in the pre-incubation assay for L. gasseri 1 and L. paracasei 11. Overall, Lactobacillus strains showed significant anti-Candida activity, and their biosurfactants exhibited considerable anti-adhesion and antibiofilm activity against C. albicans. To be considered safe for use in vivo, the safety of biosurfactant (BS) should be investigated using cytotoxicity assays.
Camilla Itapary Dos Santos; Yasmine Ramos França; Carmem Duarte Lima Campos; Maria Rosa Quaresma Bomfim; Bruna Oliveira Melo; Rodrigo Assunção Holanda; Vera Lucia Santos; Sílvio Gomes Monteiro; Eduardo Moffa; Andrea Souza Monteiro; Cristina Andrade Monteiro; Valério Monteiro-Neto. Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates. Pathogens 2019, 8, 150 .
AMA StyleCamilla Itapary Dos Santos, Yasmine Ramos França, Carmem Duarte Lima Campos, Maria Rosa Quaresma Bomfim, Bruna Oliveira Melo, Rodrigo Assunção Holanda, Vera Lucia Santos, Sílvio Gomes Monteiro, Eduardo Moffa, Andrea Souza Monteiro, Cristina Andrade Monteiro, Valério Monteiro-Neto. Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates. Pathogens. 2019; 8 (3):150.
Chicago/Turabian StyleCamilla Itapary Dos Santos; Yasmine Ramos França; Carmem Duarte Lima Campos; Maria Rosa Quaresma Bomfim; Bruna Oliveira Melo; Rodrigo Assunção Holanda; Vera Lucia Santos; Sílvio Gomes Monteiro; Eduardo Moffa; Andrea Souza Monteiro; Cristina Andrade Monteiro; Valério Monteiro-Neto. 2019. "Antifungal and Antivirulence Activity of Vaginal Lactobacillus Spp. Products against Candida Vaginal Isolates." Pathogens 8, no. 3: 150.
Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower richness and diversity than others, using the Shannon and Simpson indices. By the principal coordinate analysis (PCoA) it was possible to observe the clustering of the bacterial community of most wells in response of the presence of PAH contamination. The significance analysis using edgeR package of the R program showed variation in the abundance of some Operational Taxonomic Units (OTUs) of contaminated wells compared to uncontaminated ones. Taxons enriched in the contaminated wells were correlated positively (p < 0.05) with the hydrocarbons, according to redundancy analysis (RDA). All these enriched taxa have been characterized as PAH degrading agents, such as the genus Comamonas, Geobacter, Hydrocarboniphaga, Anaerolinea and Desulfomonile. Additionally, it was possible to predict, with the PICRUSt program, a greater proportion of pathways and genes related to the degradation of PAHs in the wells with higher contamination levels. We conclude that the contaminants promoted the enrichment of several groups of degrading bacteria in the area, which strengthens the feasibility of applying biostimulation as an aquifer remediation strategy.
Aline Daniela Lopes Júlio; Ubiana De Cássia Mourão Silva; Julliane Dutra Medeiros; Daniel Kumazawa Morais; Vera Lúcia Dos Santos. Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation. Scientific Reports 2019, 9, 1 -14.
AMA StyleAline Daniela Lopes Júlio, Ubiana De Cássia Mourão Silva, Julliane Dutra Medeiros, Daniel Kumazawa Morais, Vera Lúcia Dos Santos. Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation. Scientific Reports. 2019; 9 (1):1-14.
Chicago/Turabian StyleAline Daniela Lopes Júlio; Ubiana De Cássia Mourão Silva; Julliane Dutra Medeiros; Daniel Kumazawa Morais; Vera Lúcia Dos Santos. 2019. "Metataxonomic analyses reveal differences in aquifer bacterial community as a function of creosote contamination and its potential for contaminant remediation." Scientific Reports 9, no. 1: 1-14.
Although the most widely accepted mechanism of action for polymyxins is related to bacterial lysis via disruption, we hypothesized that this antimicrobial drug class could have other effects on Pseudomonas aeruginosa planktonic and sessile cells. Little is known regarding oxidative burst and zeta potential (ZP) data associated with the interaction between polymyxin B and P. aeruginosa cells. The present study evaluated endogenous reactive oxygen species (ROS) production and changes in the net charges of biofilm and planktonic cells in response to polymyxin B. Polymyxin B induced concentration-dependent killing at all concentrations tested in planktonic and sessile cells from P. aeruginosa strains. Sublethal concentrations of polymyxin B induced oxidative burst. ROS production was higher in resistant planktonic cells than in biofilm cells but this was not observed for susceptible cells. Moreover, no net surface charge alterations were observed in planktonic cells from a susceptible strain treated with polymyxin B, but a significant increase of ZP was noted in planktonic cells from a resistant strain. Oxidative burst generated by planktonic and sessile cells from P. aeruginosa strains against polymyxin B indicates that ROS may have an important role in the mechanism of action of this drug. ZP data revealed that electrostatic interactions of the cationic peptide with the anionic surface of the cells are strain-dependent. Therefore, we suggested that the intracellular effects of polymyxin B should be further investigated to understand polymyxin B-induced stress in P. aeruginosa.
Marlucy Rodrigues Lima; Gabriella Freitas Ferreira; Wallace Ribeiro Nunes Neto; Joveliane De Melo Monteiro; Áquila Rodrigues Costa Santos; Priscila Batista Tavares; Ângelo Márcio Leite Denadai; Maria Rosa Quaresma Bomfim; Vera Lúcia Dos Santos; Sirlei Garcia Marques; Andrea De Souza Monteiro. Evaluation of the interaction between polymyxin B and Pseudomonas aeruginosa biofilm and planktonic cells: reactive oxygen species induction and zeta potential. BMC Microbiology 2019, 19, 115 .
AMA StyleMarlucy Rodrigues Lima, Gabriella Freitas Ferreira, Wallace Ribeiro Nunes Neto, Joveliane De Melo Monteiro, Áquila Rodrigues Costa Santos, Priscila Batista Tavares, Ângelo Márcio Leite Denadai, Maria Rosa Quaresma Bomfim, Vera Lúcia Dos Santos, Sirlei Garcia Marques, Andrea De Souza Monteiro. Evaluation of the interaction between polymyxin B and Pseudomonas aeruginosa biofilm and planktonic cells: reactive oxygen species induction and zeta potential. BMC Microbiology. 2019; 19 (1):115.
Chicago/Turabian StyleMarlucy Rodrigues Lima; Gabriella Freitas Ferreira; Wallace Ribeiro Nunes Neto; Joveliane De Melo Monteiro; Áquila Rodrigues Costa Santos; Priscila Batista Tavares; Ângelo Márcio Leite Denadai; Maria Rosa Quaresma Bomfim; Vera Lúcia Dos Santos; Sirlei Garcia Marques; Andrea De Souza Monteiro. 2019. "Evaluation of the interaction between polymyxin B and Pseudomonas aeruginosa biofilm and planktonic cells: reactive oxygen species induction and zeta potential." BMC Microbiology 19, no. 1: 115.
Bacterial keratitis is an ocular infection that can lead to severe visual disability. Staphylococcus aureus is a major pathogen of the eye. We recently demonstrated the strong antimicrobial activity of LyeTxI-b, a synthetic peptide derived from a Lycosa erithrognatha toxin. Herein, we evaluated a topical formulation (eye drops) containing LyeTxI-b to treat resistant bacterial keratitis. Keratitis was induced with intrastromal injection of 4 × 105 cells (4 µL) in New Zealand female white rabbits. Minimum inhibitory concentration (MIC) and biofilm viability were determined. LyeTxI-b ocular toxicity was evaluated through chorioallantoic membrane and Draize tests. One drop of the formulation (LyeTxI-b 28.9 µmol/L +0.5% CMC in 0.9% NaCl) was instilled into each eye four times a day, for a week. Slit-lamp biomicroscopy analysis, corneal histopathological studies and cellular infiltrate quantification through myeloperoxidase (MPO) and N-acetylglucosaminidase (NAG) detection were performed. LyeTxI-b was very effective in the treatment of keratitis, with no signs of ocular toxicity. Planktonic bacteria MIC was 3.6 µmol/L and LyeTxI-b treatment reduced biofilm viability in 90%. LyeTxI-b eliminated bacteria and reduced inflammatory cellular activity in the eyes. Healthy and treated animals showed similar NAG and MPO levels. LyeTxI-b is a potent new drug to treat resistant bacterial keratitis, showing effective antimicrobial and anti-inflammatory activity.
Carolina Nunes Da Silva; Flavia Rodrigues Da Silva; Lays Fernanda Nunes Dourado; Pablo Victor Mendes Dos Reis; Rummenigge Oliveira Silva; Bruna Lopes Da Costa; Paula Santos Nunes; Flávio Almeida Amaral; Vera Lúcia Dos Santos; Maria Elena De Lima; Armando Da Silva Cunha Júnior. A New Topical Eye Drop Containing LyeTxI-b, A Synthetic Peptide Designed from A Lycosa erithrognata Venom Toxin, Was Effective to Treat Resistant Bacterial Keratitis. Toxins 2019, 11, 203 .
AMA StyleCarolina Nunes Da Silva, Flavia Rodrigues Da Silva, Lays Fernanda Nunes Dourado, Pablo Victor Mendes Dos Reis, Rummenigge Oliveira Silva, Bruna Lopes Da Costa, Paula Santos Nunes, Flávio Almeida Amaral, Vera Lúcia Dos Santos, Maria Elena De Lima, Armando Da Silva Cunha Júnior. A New Topical Eye Drop Containing LyeTxI-b, A Synthetic Peptide Designed from A Lycosa erithrognata Venom Toxin, Was Effective to Treat Resistant Bacterial Keratitis. Toxins. 2019; 11 (4):203.
Chicago/Turabian StyleCarolina Nunes Da Silva; Flavia Rodrigues Da Silva; Lays Fernanda Nunes Dourado; Pablo Victor Mendes Dos Reis; Rummenigge Oliveira Silva; Bruna Lopes Da Costa; Paula Santos Nunes; Flávio Almeida Amaral; Vera Lúcia Dos Santos; Maria Elena De Lima; Armando Da Silva Cunha Júnior. 2019. "A New Topical Eye Drop Containing LyeTxI-b, A Synthetic Peptide Designed from A Lycosa erithrognata Venom Toxin, Was Effective to Treat Resistant Bacterial Keratitis." Toxins 11, no. 4: 203.
Lactobacillus species produce biosurfactants that can contribute to the bacteria’s ability to prevent microbial infections associated with urogenital and gastrointestinal tracts and the skin. Here, we described the biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. The biosurfactants produced by L. jensenii P6A and L. gasseri P65 reduced the water surface tension from 72 to 43.2 mN m−1 and 42.5 mN m−1 as their concentration increased up to the critical micelle concentration (CMC) values of 7.1 and 8.58 mg mL−1, respectively. Maximum emulsifying activity was obtained at concentrations of 1 and 5 mg mL−1 for the P6A and P65 strains, respectively. The Fourier transform infrared spectroscopy data revealed that the biomolecules consist of a mixture of carbohydrates, lipids and proteins. The gas chromatography-mass spectrum analysis of L. jensenii P6A biosurfactant showed a major peak for 14-methypentadecanoic acid, which was the main fatty acid present in the biomolecule; conversely, eicosanoic acid dominated the biosurfactant produced by L. gasseri P65. Although both biosurfactants contain different percentages of the sugars galactose, glucose and ribose; rhamnose was only detected in the biomolecule produced by L. jensenii P6A. Emulsifying activities were stable after a 60-min incubation at 100 °C, at pH 2–10, and after the addition of potassium chloride and sodium bicarbonate, but not in the presence of sodium chloride. The biomolecules showed antimicrobial activity against clinical isolates of Escherichia coli and Candida albicans, with MIC values of 16 µg mL−1, and against Staphylococcus saprophyticus, Enterobacter aerogenes and Klebsiella pneumoniae at 128 µg mL−1. The biosurfactants also disrupted preformed biofilms of microorganisms at varying concentrations, being more efficient against E. aerogenes (64%) (P6A biosurfactant), and E. coli (46.4%) and S. saprophyticus (39%) (P65 biosurfactant). Both strains of lactobacilli could also co-aggregate pathogens. This report presents the first characterization of biosurfactants produced by L. jensenii P6A and L. gasseri P65. The antimicrobial properties and stability of these biomolecules indicate their potential use as alternative antimicrobial agents in the medical field for applications against pathogens that are responsible for infections in the gastrointestinal and urogenital tracts and the skin.
I. M. C. Morais; A. L. Cordeiro; G. S. Teixeira; V. S. Domingues; R. M. D. Nardi; A. S. Monteiro; R. J. Alves; E. P. Siqueira; V. L. Santos. Biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. Microbial Cell Factories 2017, 16, 1 -15.
AMA StyleI. M. C. Morais, A. L. Cordeiro, G. S. Teixeira, V. S. Domingues, R. M. D. Nardi, A. S. Monteiro, R. J. Alves, E. P. Siqueira, V. L. Santos. Biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65. Microbial Cell Factories. 2017; 16 (1):1-15.
Chicago/Turabian StyleI. M. C. Morais; A. L. Cordeiro; G. S. Teixeira; V. S. Domingues; R. M. D. Nardi; A. S. Monteiro; R. J. Alves; E. P. Siqueira; V. L. Santos. 2017. "Biological and physicochemical properties of biosurfactants produced by Lactobacillus jensenii P6A and Lactobacillus gasseri P65." Microbial Cell Factories 16, no. 1: 1-15.
Phosphate fertilization is a common practice in agriculture worldwide, and several commercial products are widely used. Triple superphosphate (TSP) is an excellent soluble P source. However, its high cost of production makes the long-term use of crude rock phosphate (RP) a more attractive alternative in developing countries, albeit its influence on plant-associated microbiota remains unclear. Here, we compared long-term effects of TSP and RP fertilization on the structure of maize rhizosphere microbial community using next generation sequencing. Proteobacteria were dominant in all conditions, whereas Oxalobacteraceae (mainly Massilia and Herbaspirillum) was enriched in the RP-amended soil. Klebsiella was the second most abundant taxon in the RP-treated soil. Burkholderia sp and Bacillus sp were enriched in the RP-amended soil when compared to the TSP-treated soil. Regarding fungi, Glomeromycota showed highest abundance in RP-amended soils, and the main genera were Scutellospora and Racocetra. These taxa are already described as important for P solubilization/acquisition in RP-fertilized soil. Maize grown on TSP and RP-treated soil presented similar productivity, and a positive correlation was detected for P content and the microbial community of the soils. The results suggest changes of the microbial community composition associated to the type of phosphate fertilization. Whilst it is not possible to establish causality relations, our data highlights a few candidate taxa that could be involved in rock phosphate solubilization and plant growth promotion. Moreover, this can represent a shorter path for further studies aiming the isolation and validation of the taxa described here concerning P release on the soil plant system and their use as bioinoculants.
Ubiana C. Silva; Julliane D. Medeiros; Laura R. Leite; Daniel Morais; Sara Cuadros-Orellana; Christiane A. Oliveira; Ubiraci G. De Paula Lana; Eliane A. Gomes; Vera L. Dos Santos. Long-Term Rock Phosphate Fertilization Impacts the Microbial Communities of Maize Rhizosphere. Frontiers in Microbiology 2017, 8, 1266 .
AMA StyleUbiana C. Silva, Julliane D. Medeiros, Laura R. Leite, Daniel Morais, Sara Cuadros-Orellana, Christiane A. Oliveira, Ubiraci G. De Paula Lana, Eliane A. Gomes, Vera L. Dos Santos. Long-Term Rock Phosphate Fertilization Impacts the Microbial Communities of Maize Rhizosphere. Frontiers in Microbiology. 2017; 8 ():1266.
Chicago/Turabian StyleUbiana C. Silva; Julliane D. Medeiros; Laura R. Leite; Daniel Morais; Sara Cuadros-Orellana; Christiane A. Oliveira; Ubiraci G. De Paula Lana; Eliane A. Gomes; Vera L. Dos Santos. 2017. "Long-Term Rock Phosphate Fertilization Impacts the Microbial Communities of Maize Rhizosphere." Frontiers in Microbiology 8, no. : 1266.
The availability of antimicrobial peptides from several different natural sources has opened an avenue for the discovery of new biologically active molecules. To the best of our knowledge, only two peptides isolated from the frog Leptodactylus labyrinthicus, namely pentadactylin and ocellatin-F1, have shown antimicrobial activities. Therefore, in order to explore the antimicrobial potential of this species, we have investigated the biological activities and membrane interactions of three peptides isolated from the anuran skin secretion. Three peptide primary structures were determined by automated Edman degradation. These sequences were prepared by solid-phase synthesis and submitted to activity assays against gram-positive and gram-negative bacteria and against two fungal strains. The hemolytic properties of the peptides were also investigated in assays with rabbit blood erythrocytes. The conformational preferences of the peptides and their membrane interactions have been investigated by circular dichroism spectroscopy and liposome dye release assays. The amino acid compositions of three ocellatins were determined and the sequences exhibit 100% homology for the first 22 residues (ocellatin-LB1 sequence). Ocellatin-LB2 carries an extra Asn residue and ocellatin-F1 extra Asn-Lys-Leu residues at C-terminus. Ocellatin-F1 presents a stronger antibiotic potential and a broader spectrum of activities compared to the other peptides. The membrane interactions and pore formation capacities of the peptides correlate directly with their antimicrobial activities, i.e., ocellatin-F1 > ocellatin-LB1 > ocellatin-LB2. All peptides acquire high helical contents in membrane environments. However, ocellatin-F1 shows in average stronger helical propensities. The obtained results indicate that the three extra amino acid residues at the ocellatin-F1 C-terminus play an important role in promoting stronger peptide-membrane interactions and antimicrobial properties. The extra Asn-23 residue present in ocellatin-LB2 sequence seems to decrease its antimicrobial potential and the strength of the peptide-membrane interactions.
Karla A. G. Gusmão; Daniel M. Dos Santos; Virgílio M. Santos; María Esperanza Cortés; Pablo V. M. Reis; Vera L. Santos; Dorila Piló-Veloso; Rodrigo M. Verly; Maria Elena De Lima; Jarbas M. Resende. Ocellatin peptides from the skin secretion of the South American frog Leptodactylus labyrinthicus (Leptodactylidae): characterization, antimicrobial activities and membrane interactions. Journal of Venomous Animals and Toxins including Tropical Diseases 2017, 23, 1 -14.
AMA StyleKarla A. G. Gusmão, Daniel M. Dos Santos, Virgílio M. Santos, María Esperanza Cortés, Pablo V. M. Reis, Vera L. Santos, Dorila Piló-Veloso, Rodrigo M. Verly, Maria Elena De Lima, Jarbas M. Resende. Ocellatin peptides from the skin secretion of the South American frog Leptodactylus labyrinthicus (Leptodactylidae): characterization, antimicrobial activities and membrane interactions. Journal of Venomous Animals and Toxins including Tropical Diseases. 2017; 23 (1):1-14.
Chicago/Turabian StyleKarla A. G. Gusmão; Daniel M. Dos Santos; Virgílio M. Santos; María Esperanza Cortés; Pablo V. M. Reis; Vera L. Santos; Dorila Piló-Veloso; Rodrigo M. Verly; Maria Elena De Lima; Jarbas M. Resende. 2017. "Ocellatin peptides from the skin secretion of the South American frog Leptodactylus labyrinthicus (Leptodactylidae): characterization, antimicrobial activities and membrane interactions." Journal of Venomous Animals and Toxins including Tropical Diseases 23, no. 1: 1-14.
Because of the inherent immunosuppression of cancer patients opportunistic infections by Candida spp, occur frequently. This study aimed to identify Candida species in the oral mucosa of 59 patients with orogastric cancer (OGC) and to analyze the immunological phenotype of these patients. The yeasts were identified by MALDI-TOF mass spectrometry (MS). For all isolates, we performed phospholipases and proteinases assays, in vitro adherence to buccal epithelial cells (BEC), minimum inhibitory concentration of antifungal drugs and determined the cytokine profile by Cytometric Bead Array flow citometry assay. C. albicans was the most prevalent species in OGC patients (51.6 %) and control group (66.7 %). Candida spp. strains isolated from OGC patients exhibited better adherence to BEC (p = 0.05) than did the control group. Phospholipases production by Candida strains from OGC patients was lower (51.6 %) than in the control group (61.9 %). Proteinases were detected in 41.9 % and 4.8 % of the yeasts from OGC patients and control group, respectively. Significant differences were found in the serum of OGC patients compared to the control group for IL-2, IL-10, TNF-α, IFN-γ and IL-17. The results of this work suggest increased virulence of yeasts isolated from OGC patients and, that this may interfere with the immune phenotype.
Lourimar Viana Nascimento F. De Sousa; Vera Lúcia Santos; Andrea De Souza Monteiro; Marcus Vinicíus Dias-Souza; Sirlei Garcia Marques; Elaine Speziali De Faria; Elaine Alves De Oliveira Assunção; Simone Gonçalves Dos Santos; Juan Moises Zonis; Daniel Gomes De Alvarenga; Rodrigo Assunção De Holanda; Jaqueline Gontijo De Sousa; Kênia Valéria Dos Santos; Maria Aparecida De Resende Stoianoff. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype. BMC Infectious Diseases 2016, 16, 1 -12.
AMA StyleLourimar Viana Nascimento F. De Sousa, Vera Lúcia Santos, Andrea De Souza Monteiro, Marcus Vinicíus Dias-Souza, Sirlei Garcia Marques, Elaine Speziali De Faria, Elaine Alves De Oliveira Assunção, Simone Gonçalves Dos Santos, Juan Moises Zonis, Daniel Gomes De Alvarenga, Rodrigo Assunção De Holanda, Jaqueline Gontijo De Sousa, Kênia Valéria Dos Santos, Maria Aparecida De Resende Stoianoff. Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype. BMC Infectious Diseases. 2016; 16 (1):1-12.
Chicago/Turabian StyleLourimar Viana Nascimento F. De Sousa; Vera Lúcia Santos; Andrea De Souza Monteiro; Marcus Vinicíus Dias-Souza; Sirlei Garcia Marques; Elaine Speziali De Faria; Elaine Alves De Oliveira Assunção; Simone Gonçalves Dos Santos; Juan Moises Zonis; Daniel Gomes De Alvarenga; Rodrigo Assunção De Holanda; Jaqueline Gontijo De Sousa; Kênia Valéria Dos Santos; Maria Aparecida De Resende Stoianoff. 2016. "Isolation and identification of Candida species in patients with orogastric cancer: susceptibility to antifungal drugs, attributes of virulence in vitro and immune response phenotype." BMC Infectious Diseases 16, no. 1: 1-12.
Biodiesel industry wastes were evaluated as supplements for lipase production by Moniliella spathulata R25L270, which is newly identified yeast with great lipolytic potential. Macaúba cake (MC), used for the first time in this work as inducer to produce lipases, and residual oil (RO) were mixed to maximise enzyme production. The lipase secreted was biochemically characterised. The best ratio for the mixture (MC:RO) was 0.66:0.34 and the fitted values for lipase activity and total protein concentration were 0.98 U mL−1 and 0.356 mg mL−1, respectively. Maximum activity obtained (2.47 U mL−1) was achieved at 31.5°C and pH 6.7, and the enzyme was stable in this condition. A novel enzyme was purified and identified for the first time by mass spectrometry. The lipase efficiently hydrolysed different natural oils and exhibited selectivity in the production of eicosapentaenoic acid from fish oil. The use of MC and RO as a supplement to produce the new lipase from M. spathulata R25L270 may be one alternative for reducing lipase production costs and simultaneously adding value to biodiesel industry residues. The potential application of the lipase in the oleochemical industry was demonstrated by its pH and temperature stabilities and selective hydrolysis.
Lívia T. A. Souza; Jamil S. Oliveira; Marina Q. R. B. Rodrigues; Vera L. Dos Santos; Benevides C. Pessela; Rodrigo R. Resende. Macaúba (Acrocomia aculeata) cake from biodiesel processing: a low-cost substrate to produce lipases from Moniliella spathulata R25L270 with potential application in the oleochemical industry. Microbial Cell Factories 2015, 14, 1 -13.
AMA StyleLívia T. A. Souza, Jamil S. Oliveira, Marina Q. R. B. Rodrigues, Vera L. Dos Santos, Benevides C. Pessela, Rodrigo R. Resende. Macaúba (Acrocomia aculeata) cake from biodiesel processing: a low-cost substrate to produce lipases from Moniliella spathulata R25L270 with potential application in the oleochemical industry. Microbial Cell Factories. 2015; 14 (1):1-13.
Chicago/Turabian StyleLívia T. A. Souza; Jamil S. Oliveira; Marina Q. R. B. Rodrigues; Vera L. Dos Santos; Benevides C. Pessela; Rodrigo R. Resende. 2015. "Macaúba (Acrocomia aculeata) cake from biodiesel processing: a low-cost substrate to produce lipases from Moniliella spathulata R25L270 with potential application in the oleochemical industry." Microbial Cell Factories 14, no. 1: 1-13.
The study was planned to quantify the distribution of bacteria between bulk water and biofilm formed on different materials in an industrial scale cooling tower system of an oil refinery operating with clarified and chlorinated freshwater (CCW) or chlorinated tertiary effluent (TRW) as makeup water. The sessile and planktonic heterotrophic bacteria and Pseudomonas aeruginosa densities were significantly higher in the cooling tower supplied with clarified and chlorinated freshwater (CTCW) (p 3 colony-forming unit (CFU) cm−1) and planktonic (0–2.36 × 103 CFU mL−1) microbiota remained below of the maximum values of reference used by water treatment companies as indicative of efficient microbial control. These data indicate that the strategies of the water treatment station (WTS) (free chlorine) and industrial wastewater treatment station (IWTS) followed by reverse electrodialysis system (RES) (free chlorine plus chloramine) were effective for the microbiological control of the two makeup water sources.
Vera Lúcia Dos Santos; Andréa Azevedo Veiga; Rafael Silva Mendonça; Andrea Lima Alves; Sérgio Pagnin; Vânia M. J. Santiago. Reuse of refinery’s tertiary-treated wastewater in cooling towers: microbiological monitoring. Environmental Science and Pollution Research 2014, 22, 2945 -2955.
AMA StyleVera Lúcia Dos Santos, Andréa Azevedo Veiga, Rafael Silva Mendonça, Andrea Lima Alves, Sérgio Pagnin, Vânia M. J. Santiago. Reuse of refinery’s tertiary-treated wastewater in cooling towers: microbiological monitoring. Environmental Science and Pollution Research. 2014; 22 (4):2945-2955.
Chicago/Turabian StyleVera Lúcia Dos Santos; Andréa Azevedo Veiga; Rafael Silva Mendonça; Andrea Lima Alves; Sérgio Pagnin; Vânia M. J. Santiago. 2014. "Reuse of refinery’s tertiary-treated wastewater in cooling towers: microbiological monitoring." Environmental Science and Pollution Research 22, no. 4: 2945-2955.
Yeasts capable of growth on xylose were isolated from macaúba (Acrocomia aculeata) fruit, a Brazilian palm tree with great potential for use as biodiesel feedstock production. Candida boidinii UFMG14 strain achieved the highest ethanol production (5g/L) and was chosen to ferment macaúba presscake hemicellulosic hydrolysate (MPHH). The MPHH was produced by the first time in this work and the resultant fivefold concentrate showed considerable sugar content (52.3 and 34.2g/L xylose and glucose, respectively) and low furfural (0.01g/L) and hydroxymethylfurfural (0.15g/L) concentrations. C. boidinii UFMG14 fermentation was evaluated in supplemented and non-supplemented MPHH containing either 10 or 25g/L of xylose. The maximum ethanol production (12g/L) was observed after 48h of fermentation. The ethanol yield was significantly affected by supplementation and concentration of MPHH while ethanol productivity was affected only by MPHH concentration. This is the first study demonstrating theC. boidinii potential for ethanol production from hemicellulose byproducts
D.B. Gonçalves; A.F. Batista; M.Q.R.B. Rodrigues; K.M.V. Nogueira; V.L. Santos. Ethanol production from macaúba ( Acrocomia aculeata ) presscake hemicellulosic hydrolysate by Candida boidinii UFMG14. Bioresource Technology 2013, 146, 261 -266.
AMA StyleD.B. Gonçalves, A.F. Batista, M.Q.R.B. Rodrigues, K.M.V. Nogueira, V.L. Santos. Ethanol production from macaúba ( Acrocomia aculeata ) presscake hemicellulosic hydrolysate by Candida boidinii UFMG14. Bioresource Technology. 2013; 146 ():261-266.
Chicago/Turabian StyleD.B. Gonçalves; A.F. Batista; M.Q.R.B. Rodrigues; K.M.V. Nogueira; V.L. Santos. 2013. "Ethanol production from macaúba ( Acrocomia aculeata ) presscake hemicellulosic hydrolysate by Candida boidinii UFMG14." Bioresource Technology 146, no. : 261-266.
The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial wastes.
Andrea De Souza Monteiro; Vitor Domingues; Marcus Vd Souza; Ivana Lula; Daniel Bonoto Gonçalves; Ezequias Pessoa De Siqueira; Vera Lúcia Dos Santos. Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2. Biotechnology for Biofuels 2012, 5, 29 -29.
AMA StyleAndrea De Souza Monteiro, Vitor Domingues, Marcus Vd Souza, Ivana Lula, Daniel Bonoto Gonçalves, Ezequias Pessoa De Siqueira, Vera Lúcia Dos Santos. Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2. Biotechnology for Biofuels. 2012; 5 (1):29-29.
Chicago/Turabian StyleAndrea De Souza Monteiro; Vitor Domingues; Marcus Vd Souza; Ivana Lula; Daniel Bonoto Gonçalves; Ezequias Pessoa De Siqueira; Vera Lúcia Dos Santos. 2012. "Bioconversion of biodiesel refinery waste in the bioemulsifier by Trichosporon mycotoxinivorans CLA2." Biotechnology for Biofuels 5, no. 1: 29-29.