Poplar, as the most widely cultivated fast-growing tree species in the middle latitude plain, provides important wood resources and plays an important role in mitigating climate change. In order to understand the response of growth, biomass production, carbon storage to poplar clones, planting spacings, and their interaction, a field trial was established in 2007. In 2018, we destructively harvested 24 sample trees for biomass measurements and stem analyses. Biomass production and carbon storage for the single tree of three clones enhanced as planting spacing increasing at the age of 13, but both the biomass production and carbon storage of clones NL-895 and NL-95 were higher than the clone NL-797 at the spacings of 6 × 6 m and 5 × 5 m. The average carbon concentration of the tested clones was in the order of stem > branches > leaves, and showed significant variation between different components (p < 0.05). Large spacing stimulated more biomass to be partitioned to the canopy. Based on the prediction values of tree volume growth by established Chapman–Richards models, the quantitative maturity ages of stand volume varied among the investigating plantations, ranging from 14 to 17 years old. Our results suggest that the selecting clones NL-895 and NL-95 with 6 × 6 m spacing would be recommended at similar sites for future poplar silviculture of larger diameter timber production, as well as for carbon sequestration.