The Mekong River is one of the world’s largest rivers, unparalleled in terms of its biodiversity and ecosystem services. As in other regions, sufficient water quality is required to support diverse organisms, habitats, and ecosystems, but in the Mekong region, water quality has not been well studied. Based on biological and physical-chemical data collected over the last two decades, we evaluated spatial-temporal water quality of the Lower Mekong Basin (LMB) using biotic and abiotic assessment metrics. We found that during the 2000s, water quality in the LMB was unpolluted, with “very good” metrics for tributary rivers and “good” status for mainstem rivers. However, during the last decade, water quality has been degraded in the LMB, particularly near Vientiane City; the Sekong, Sesan, and Srepok (3S) Rivers; the Tonle Sap Lake system; and the Mekong Delta. Water quality degradation likely corresponds to flow alteration, erosion, sediment trapping, and point and non-point wastewater, which have occurred from rapid hydropower development, deforestation, intensive agriculture, plastic pollution, and urbanization. Regular biomonitoring, physical-chemical water quality assessment, transparent data sharing, and basin-wide water quality standards or management are needed to sustain water quality to support biodiversity and ecosystem function in the LMB.

The Lower Mekong Basin (LMB) is a key biodiversity hotspot. To facilitate conservation and management, we examine mollusc biodiversity patterns and distribution along LMB’s longitudinal gradients, identify environmental drivers, and discuss the importance of these drivers to management. Cluster analysis, redundancy analysis (RDA), and variation partitioning were conducted using mollusc data collected from 63 sampling sites. Results indicated that species diversity is dominated by gastropods (61%) and bivalves (39%) and feeding trait diversity by scrapers (52%) and filter-collectors (37%). Only 48 species (49%) out of 98 taxa have been assessed by the International Union for Conservation of Nature (IUCN) including a growing number of invasive species. The lack of complete, up-to-date information highlights the need for more research on both native and alien species. Cluster analysis revealed a clear mollusc biodiversity structure along the LMB’s longitudinal segments. Diversity was lowest in upstream tributaries, increased in upstream main channels, and was highest in downstream channels and the Mekong delta, the exception being the observed high gastropod abundance in Chi-Mun river mouth and Luang Prabang areas. The RDA and variation partitioning demonstrated that combined physical–chemical and climatic conditions are the key drivers of biodiversity patterns. Given the potential spread of invasive alien species and increasing anthropogenic impacts, further ecological research, regular monitoring, and adaptive management are needed to sustain mollusc biodiversity and associated ecosystem services, which contribute to food security, nutrition, and livelihoods in the LMB.

Tonle Sap Lake (TSL) is a highly productive system and hosts a high fish diversity and is of paramount importance for sustaining protein supply for over 15 million Cambodians. Nevertheless, the ecology and factors influencing the spatial distribution of many fishes within the lake remain poorly understood. Using commercial fishing lot catch data from 1994/1995 to 1999/2000, fishing seasons and environmental data (land cover and bioclimatic variables), we describe spatial distribution of the eight most commercially important fish species, and investigate the effects of environmental factors on their distributions in the TSL. We found a strong variability in fish biomass across areas and between species. Specifically, Channa micropeltes was most abundant in the southern and northern sections of the TSL. Channa striata and Trichopodus microlepis were more common in the northern part of the TSL. Cyclocheilos enoplos, Barbonymus gonionotus, Pangasianodon hypophthalmus, and Gymnostomus spp. were abundant in the southern areas of the TSL while Phalacronotus spp. were abundant in few areas in both the north and the south. Flooded forest positively explained the variation in the biomass of P. hypophthalmus, C. striata, C. enopolos, and Phalacronotus spp. Likewise, the lake’s open water positively affects the biomass of P. hypophthalmus, C. enopolos, and Phalacronotus spp., while the agricultural field negatively impacts Gymnostomus spp. biomass distribution. We also found that some areas consistently hosted high fish biomass (e.g., lot 2, Kampong Thom; lot 6, Pursat; lot 2, Battambang, etc.). We, therefore, suggest that fisheries management and conservation planning focus on those areas, considering those areas significance as core fish habitat and important for catching fish.

Inland fisheries management in Cambodia has undergone two major policy reforms over the last two decades. These reforms led to the abolishment of a century-old commercial fishing lot system in 2012 and the establishment of new fish sanctuary and community fishing areas. However, the status of fisheries and fish assemblages following the reforms is not well understood. Here, we investigated the temporal changes in fish catch weight and fish assemblage structure for the period 1995–2000 before fishing lot abolishment (BLA) and for the period 2012–2015 after the removal of all fishing lots (after lot abolishment-ALA) using time-series fish catch data recorded from the Tonle Sap Lake (TSL), one of the world largest inland fisheries. We found (i) mean catch trends vary seasonally, with stable catch trends during the BLA and decreasing catch trends during the ALA and (ii) significant shifts in fish assemblage composition, notably a shift from large-bodied, migratory, and/or predatory species during the BLA toward more short-distance migratory and/or floodplain, small-bodied species during the ALA. Fishing lot abolishment coincided with substantial changes to floodplain habitats and increases in fishing pressure, threatening TSL fish stocks. Flow alterations caused by dams and climate change may exacerbate the problem. Therefore, to realize the fisheries reform objectives, it is imperative to strengthen the fisheries’ governance and management system, including effective law enforcement, institutional strengthening, improved planning, cooperation, and coordination as well as clearly defined roles and responsibilities among concerned stakeholders at all levels.

Tonle Sap lake-river floodplain ecosystem (TSE) is one of the world’s most productive freshwater systems. Changes in hydrology, climate, population density, and land use influence water quality in this system. We investigated long term water quality dynamics (22 years) in space and time and identified potential changes in nutrient limitation based on nutrient ratios of inorganic nitrogen and phosphorus. Water quality was assessed at five sites highlighting the dynamics in wet and dry seasons. Predictors of water quality included watershed land use, climate, population, and water level. Most water quality parameters varied across TSE, except pH and nitrate that remained constant at all sites. In the last decade, there is a change in the chemical nutrient ratio suggesting that nitrogen may be the primary limiting nutrient across the system. Water quality was strongly affected by development in the watershed i.e., flooded forest loss, climatic variation, population growth, and change in water level. Seasonal variations of water quality constituents were driven by precipitation and hydrology, notably the Mekong’s distinct seasonal flood pulse.