This study aimed to investigate the impact of cold stress and priming on photosynthesis in the early development of maize and soybean, crops with diverse photosynthetic pathways. The main objectives were to determine the effect of cold stress on chlorophyll a fluorescence parameters and spectral reflectance indices, to determine the effect of cold stress priming and possible stress memory and to determine the relationship between different parameters used in determining the stress response. Fourteen maize inbred lines and twelve soybean cultivars were subjected to control, cold stress, and priming followed by cold stress in a walk-in growth chamber. Measurements were conducted using a portable fluorometer and a handheld reflectance instrument. Cold stress induced an overall downregulation of PSII-related specific energy fluxes and efficiencies, the inactivation of RCs resulting in higher energy dissipation, and electron transport chain impairment in both crops. Spectral reflectance indices suggested cold stress resulted in pigment differences between crops. The effect of priming was more pronounced in maize than in soybean with mostly a cumulatively negative effect. However, priming stabilized the electron trapping efficiency and upregulated the electron transfer system in maize, indicating an adaptive response. Overall, this comprehensive analysis provides insights into the complex physiological responses of maize and soybean to cold stress, emphasizing the need for further genotype-specific cold stress response and priming effect research.

Measuring the soil water content (SWC) is a fundamental component of the sustainable management of water resources, soil preservation, and high irrigation efficiency. Non-destructive SWC measurements using soil moisture sensors (SMSs) enables timely irrigation and reduces overirrigation and water stress. Within this context, the performance of four commercial single-point soil moisture sensors (Watermark and tensiometer (Irrometer Company, Inc., Riverside, CA, USA), SM150 (Delta-T Devices, Cambridge, UK)), FieldScout TDR300 (Spectrum Technologies, Aurora, IL, USA) and one soil profile PR2 probe (Delta-T Devices, Cambridge, UK) were tested under anthropogenic eutric cambisol with a silty clay loamy texture (20, 30, and 40 cm) to evaluate accuracy and sensitivity to changes in the SWC in an irrigated apple orchard. The Watermark and tensiometer were additionally tested in the laboratory to convert soil water tension (kPa) to the volumetric soil water content (%vol.). In general, all tested SMSs responded to changes in the SWC, with sensor-to-sensor differences. The Watermark and tensiometer underestimated the SWC, while the TDR overestimated the SWC. The SM150 and PR2 showed high accuracy, i.e., SM150—RMSE-2.24 (20 cm), 2.18 (30 cm) and 2.34 (40 cm), MSE—5.02 (20 cm), 2.93 (30 cm) and 1.89 (40 cm), and PR2—RMSE-1.8 (20 cm), 1.3 (30 cm) and 1.55 (40 cm), MSE-3.23 (20 cm), 1.7 (30 cm) and 2.39 (40 cm) at all observed soil depths.

This study examined the photosynthetic responses of two sunflower hybrids to elevated temperatures and excess light intensity in the flowering stage by measuring the chlorophyll a fluorescence (ChlF) under morning and afternoon field conditions to determine the photosynthetic pigment contents and the relative accumulation of photosynthetic proteins. The morning environmental conditions were considered optimal, while the afternoon was characterised by elevated temperatures and excess light intensity. The minimum fluorescence intensity (F₀), the electron-flux-reducing end electron acceptors at the photosystem I acceptor side per reaction centre (RE₀/RC), and the D1 protein had significant, high, and positive correlations with the environmental conditions, which indicates that they were the most useful in the sunflower-stress-response research. In hybrid 7, the elevated temperatures and the excess light intensity resulted in the inactivation of the oxygen-evolving complex, which was indicated by the positive L, K, and J steps, the increase in the maximum quantum yield of PSII (TR₀/ABS), the decrease in the electron transport further than the primary acceptor Q_A (ET₀/(TR₀-ET₀)), the reduction in the performance index (PI_ABS), and the higher relative accumulation of the light-harvesting complex of the photosystem (LHCII). Hybrid 4 had smaller changes in the fluorescence curves in phases O–J and J–I, and especially in steps L, K, J, and I, and a higher PI_ABS, which indicates a more efficient excitation energy under the unfavourable conditions. As the tested parameters were sensitive enough to determine the significant differences between the sunflower hybrids in their photosynthetic responses to the elevated temperatures and excess light intensity in the flowering stage, they can be considered useful selection criteria. The development of more adaptable sunflower hybrids encourages sustainable sunflower production under stressful growing conditions.

Global warming and the associated climate change are imposing abiotic stress on plants. Abiotic factors are crucial for plant productivity, survival, and reproduction. Eight sunflower hybrids were tested in conditions of different water availability and with combinations of different temperatures and irradiation. The changes in the photosynthetic efficiency were measured in the morning (control conditions: 2013, 25.8 °C and 349.1 W m⁻²; 2014, 21.8 °C and 296.4 W m⁻²) and afternoon (the combination of increased temperatures and high irradiation: 2013, 34 °C and 837.9 W m⁻²; 2014, 29.4 °C and 888.9 W m⁻²) at a flowering stage in rainfed or irrigated conditions. The measurement time (morning and afternoon conditions) had a statistically significant effect on all the tested parameters. The performance index (PI_ABS) in 2013 and the maximum quantum yield of photosystem II (TR₀/ABS) in 2014 are the only parameters significantly affected by the irrigation. As a result of the combined effect of increased temperatures and high irradiation, PI_ABS values decreased by 73–92% in rainfed conditions and by 63–87% in irrigated conditions in 2013, depending on the hybrid, while in 2014, the decrease varied between 70 and 86%. The TR₀/ABS decrease was 7–17% in 2013, depending on the hybrid, and 6–12% in 2014, both in rainfed and irrigated conditions. The principal component analysis confirmed the effect of the combination of increased temperatures and high irradiation on hybrids, sorting them exclusively according to the time of measurement. All investigated parameters highly fluctuated between hybrids but without observable trends for the morning and afternoon conditions, as well as for irrigation. Plants’ reaction to the combination of increased temperatures and high irradiation manifested as a change in their photosynthetic efficiency, i.e., the photosynthetic apparatus’ functioning was impaired.

The reason for this examination is today’s wide usage of chlorophyll a fluorescence (ChlF) among researchers worldwide to measure photosynthetic efficiency. Although the instructions of the ChlF measuring device clearly emphasize the need for methodology adjustments, depending on the specific plant species, many researchers use the usual 30 min of dark adaptation before measurement. Namely, before any ChlF measurement, it is necessary to determine the specific duration of the leaf adaptation to the conditions of darkness of each plant tissue. Because of the numerous uses of the ChlF measurements, we decided to conduct this research to determine whether the appearance of the curves and parameter values depend on the time of sunflower leaf tissue adaptation to dark conditions. Therefore, this research aimed to examine the optimal adaptation time of sunflower tissue to dark conditions to obtain timely precise measurements and credible appearance of ChlF transient curves as well as accurate parameter values. The research was carried out on the sunflower hybrid Luka with 0, 15, 30, 45, 60, 75 and 90 min of dark adaptation in the vegetative, budding and flowering stages in the field conditions. According to the analyzed transient curves and parameters, it was determined that sunflower leaves should be kept in dark conditions for at least 15 min before the measurement of ChlF, which leads to the complete oxidation of PSII and the electron transport chain prior to a saturating pulse of light.

The potential of soybean for food, feed, and pharmaceutical industry arises from the composition of its seed. Since European countries import 95% of the annual demand for soybean grains, meal, and oil, causing an enormous trade deficit, the governments in Europe had started to introduce additional incentives to stimulate soybean cropping. To rebalance the sources of soybean supply in the future, production must be followed by continuous research to create varieties that would make European soybean more appealing to the processing industry and profitable enough to satisfy European farmers. This chapter is giving an overview of the European soybean seed quality research and an insight into soybean seed quality progress made at the Agricultural Institute Osijek, Croatia. The studies presented are mainly considering maturity groups suitable for growing in almost all European regions. The most important traits of soybean seed quality discussed are protein content and amino acid composition, oil content and fatty acid composition, soluble sugars, and isoflavones. Defining quality traits facilitates the parental selection in breeding programs aiming to improve the added value properties of final soybean products and enables the exchange of materials between different breeding and research institutions to introduce diversity, which is a prerequisite for genetic advance.