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The coronavirus disease 2019 (COVID-19) is the latest biological hazard for the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Numerous diagnostic tests for SARS-CoV-2 have been used, which are expensive and require specialized personal. So, new diagnosis strategies are being developed, looking for less expensive methods which could be used as screening for better spread control. Many researchers have described the use of saliva as a potential indicator of COVID-19, and even the same patient could carry out its collection. In this sense, this study aimed to establish specific salivary vibrational modes analyzed by attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy to detect COVID-19 biological fingerprints that allow the discrimination between COVID-19 and healthy patients. Previous written informed consent, clinical dates, laboratories, and saliva samples of COVID-19 patients (n = 255) and healthy persons (n = 1209) were obtained and analyzed through ATR-FTIR spectroscopy. Then, a multivariate linear regression model (MLRM) was developed. The COVID-19 patients showed low SaO2, cough, dyspnea, headache, and fever principally. Obesity was the main comorbidity. Various laboratory blood tests were altered. In the FTIR spectra analysis, changes in amide I and immunoglobulin regions were evidenced, and the MLRM showed clear discrimination between both groups. Specific salivary vibrational modes employing ATR-FTIR spectroscopy were established; moreover, the COVID-19 biological fingerprint in saliva was characterized, allowing the detection for COVID-19 using an MLRM, once it helps to reduce the number of variables, which could be helpful in the future development of diagnostic devices in a faster and cheaper way.
Adriana Martinez-Cuazitl; Gustavo Vazquez-Zapien; Miguel Sanchez-Brito; Jorge Limon-Pacheco; Melissa Guerrero-Ruiz; Francisco Garibay-Gonzalez; Raul Delgado-Macuil; Maria Garcia de Jesus; Miguel Corona-Perezgrovas; Armando Pereyra-Talamantes; Monica Maribel Mata-Miranda. ATR-FTIR Spectrum Analysis of Saliva Samples from COVID-19 Positive Patients. 2021, 1 .
AMA StyleAdriana Martinez-Cuazitl, Gustavo Vazquez-Zapien, Miguel Sanchez-Brito, Jorge Limon-Pacheco, Melissa Guerrero-Ruiz, Francisco Garibay-Gonzalez, Raul Delgado-Macuil, Maria Garcia de Jesus, Miguel Corona-Perezgrovas, Armando Pereyra-Talamantes, Monica Maribel Mata-Miranda. ATR-FTIR Spectrum Analysis of Saliva Samples from COVID-19 Positive Patients. . 2021; ():1.
Chicago/Turabian StyleAdriana Martinez-Cuazitl; Gustavo Vazquez-Zapien; Miguel Sanchez-Brito; Jorge Limon-Pacheco; Melissa Guerrero-Ruiz; Francisco Garibay-Gonzalez; Raul Delgado-Macuil; Maria Garcia de Jesus; Miguel Corona-Perezgrovas; Armando Pereyra-Talamantes; Monica Maribel Mata-Miranda. 2021. "ATR-FTIR Spectrum Analysis of Saliva Samples from COVID-19 Positive Patients." , no. : 1.
Diabetes is one of the four main non-communicable diseases worldwide. Despite not being a fatal disease, many complications derive from this illness that causes a drastic deterioration in the patient's health. Diabetes is a silent disease that, on many occasions, causes symptoms until the disease is already advanced, and due to the lack of education in health prevention, only a small part of the population undergoes routine laboratory studies. If this disease is detected on time, the quality of life could be improved. However, the simple facts of taking a blood sample, control studies are omitted. Besides, there is a need to sample the patient many times according to its severity and control. In the present work, we provide a novel technique based on the FTIR spectra of saliva samples to diagnose this disease. After analyzing the samples of 1,000 people, we found that it is possible to identify patients with this pathology through artificial neural networks and SVMr reliably. As it is not invasive and does not require reagents or complex processes, the proposed technique could be more agile and cheaper than traditional ones.
Miguel Sanchez-Brito; Francisco J. Luna-Rosas; Ricardo Mendoza-Gonzalez; Gustavo J. Vazquez-Zapien; Julio C. Martinez-Romo; Monica M. Mata-Miranda. Type 2 diabetes diagnosis assisted by machine learning techniques through the analysis of FTIR spectra of saliva. Biomedical Signal Processing and Control 2021, 69, 102855 .
AMA StyleMiguel Sanchez-Brito, Francisco J. Luna-Rosas, Ricardo Mendoza-Gonzalez, Gustavo J. Vazquez-Zapien, Julio C. Martinez-Romo, Monica M. Mata-Miranda. Type 2 diabetes diagnosis assisted by machine learning techniques through the analysis of FTIR spectra of saliva. Biomedical Signal Processing and Control. 2021; 69 ():102855.
Chicago/Turabian StyleMiguel Sanchez-Brito; Francisco J. Luna-Rosas; Ricardo Mendoza-Gonzalez; Gustavo J. Vazquez-Zapien; Julio C. Martinez-Romo; Monica M. Mata-Miranda. 2021. "Type 2 diabetes diagnosis assisted by machine learning techniques through the analysis of FTIR spectra of saliva." Biomedical Signal Processing and Control 69, no. : 102855.
Miguel Sánchez-Brito; Nstituto Tecnológico De Aguascalientes; Monica Maribel Mata-Miranda; Adriana Martínez-Cuazitl; Dante J. López-Mezquita; Melissa Guerrero-Ruiz; Gustavo Jesus Vazquez-Zapien. Saliva analysis using FTIR spectroscopy to detect possible SARS-CoV-2 (COVID-19) virus carriers. Mexican Journal of Biomedical Engineering 2020, 42, 6 -9.
AMA StyleMiguel Sánchez-Brito, Nstituto Tecnológico De Aguascalientes, Monica Maribel Mata-Miranda, Adriana Martínez-Cuazitl, Dante J. López-Mezquita, Melissa Guerrero-Ruiz, Gustavo Jesus Vazquez-Zapien. Saliva analysis using FTIR spectroscopy to detect possible SARS-CoV-2 (COVID-19) virus carriers. Mexican Journal of Biomedical Engineering. 2020; 42 (1):6-9.
Chicago/Turabian StyleMiguel Sánchez-Brito; Nstituto Tecnológico De Aguascalientes; Monica Maribel Mata-Miranda; Adriana Martínez-Cuazitl; Dante J. López-Mezquita; Melissa Guerrero-Ruiz; Gustavo Jesus Vazquez-Zapien. 2020. "Saliva analysis using FTIR spectroscopy to detect possible SARS-CoV-2 (COVID-19) virus carriers." Mexican Journal of Biomedical Engineering 42, no. 1: 6-9.
The World Health Organization has declared that diabetes is one of the four leading causes of death attributable to non-communicable diseases. Currently, many devices allow monitoring blood glucose levels for diabetes control based mainly on blood tests. In this paper, we propose a novel methodology based on the analysis of the Fourier Transform Infrared (FTIR) spectra of saliva using machine learning techniques to characterize controlled and uncontrolled diabetic patients, clustering patients in groups of a low, medium, and high glucose levels, and finally performing the point estimation of a glucose value. After analyzing the obtained results with Support Vector Machines (SVM), Artificial Neural Networks (ANN), and Linear Regression (LR), we found that using ANN, it is possible to carry out the characterizations mentioned above efficiently since it allowed us to identify correctly the 540 spectra that make up our database studying the region 4000-2000 cm−1.
Miguel Sánchez-Brito; Francisco J. Luna-Rosas; Ricardo Mendoza-González; Mónica M. Mata-Miranda; Julio C. Martínez-Romo; Gustavo J. Vázquez-Zapién. A machine-learning strategy to evaluate the use of FTIR spectra of saliva for a good control of type 2 diabetes. Talanta 2020, 221, 121650 .
AMA StyleMiguel Sánchez-Brito, Francisco J. Luna-Rosas, Ricardo Mendoza-González, Mónica M. Mata-Miranda, Julio C. Martínez-Romo, Gustavo J. Vázquez-Zapién. A machine-learning strategy to evaluate the use of FTIR spectra of saliva for a good control of type 2 diabetes. Talanta. 2020; 221 ():121650.
Chicago/Turabian StyleMiguel Sánchez-Brito; Francisco J. Luna-Rosas; Ricardo Mendoza-González; Mónica M. Mata-Miranda; Julio C. Martínez-Romo; Gustavo J. Vázquez-Zapién. 2020. "A machine-learning strategy to evaluate the use of FTIR spectra of saliva for a good control of type 2 diabetes." Talanta 221, no. : 121650.
Over time, the understanding of the health-disease process has evolved along with the various environments and conditions in which society has been immersed, with different forms of care, treatment and prevention. Similarly, the social and economic structure has led to inequalities in medical care, generating social subclasses regarding access to health care and physical and functional capacities. This writing briefly introduces the social subclasses in health-disease that have emerged from the socio-economic structure of capitalism.
Monica Maribel Mata-Miranda; Gustavo Jesus Vazquez-Zapien. Evolution of health, disease and disability during capitalism. International Journal of Medical and Surgical Sciences 2020, 7, 1 -3.
AMA StyleMonica Maribel Mata-Miranda, Gustavo Jesus Vazquez-Zapien. Evolution of health, disease and disability during capitalism. International Journal of Medical and Surgical Sciences. 2020; 7 (1):1-3.
Chicago/Turabian StyleMonica Maribel Mata-Miranda; Gustavo Jesus Vazquez-Zapien. 2020. "Evolution of health, disease and disability during capitalism." International Journal of Medical and Surgical Sciences 7, no. 1: 1-3.
The Organisation for Economic Co-operation and Development has listed thirteen engineered nanomaterials (ENM) in order to investigate their toxicity on human health. Silicon dioxide (SiO2) and titanium dioxide (TiO2) are included on that list and we added indium tin oxide (ITO) nanoparticles (NPs) to our study, which is not listed on OECD suggested ENM to be investigated, however ITO NPs has a high potential of industrial production. We evaluate the physicochemical properties of SiO2 NPs (10−20 nm), TiO2 nanofibers (NFs; 3 μm length) and ITO NPs (<50 nm) and the impact of protein-corona formation on cell internalization. Then, we evaluated the toxicity of uncoated ENM on human lung epithelial cells exposed to 10 and 50 μg/cm2 for 24 h. TiO2 NFs showed the highest capability to adsorb proteins onto the particle surface followed by SiO2 NPs and ITO NPs after acellular incubation with fetal bovine serum. The protein adsorption had no impact on Alizarin Red S conjugation, intrinsic properties for reactive oxygen (ROS) formation or cell uptake for all types of ENM. Moreover, TiO2 NFs induced highest cell alterations in human lung epithelial cells exposed to 10 and 50 μg/cm2 while ITO NPs induced moderated cytotoxicity and SiO2 NPs caused even lower cytotoxicity under the same conditions. DNA, proteins and lipids were mainly affected by TiO2 NFs followed by SiO2 NPs with toxic effects in protein and lipids while limited variations were detected after exposure to ITO NPs on spectra analyzed by Fourier Transform Infrared Spectroscopy.
Alejandro Déciga-Alcaraz; Estefany I. Medina-Reyes; Norma L. Delgado-Buenrostro; Carolina Rodriguez Ibarra; Adriana Ganem-Rondero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda; Jorge H. Limón-Pacheco; Claudia M. García-Cuéllar; Yesennia Sánchez-Pérez; Yolanda I. Chirino. Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production. Toxicology 2020, 442, 152545 .
AMA StyleAlejandro Déciga-Alcaraz, Estefany I. Medina-Reyes, Norma L. Delgado-Buenrostro, Carolina Rodriguez Ibarra, Adriana Ganem-Rondero, Gustavo Jesus Vazquez-Zapien, Monica Maribel Mata-Miranda, Jorge H. Limón-Pacheco, Claudia M. García-Cuéllar, Yesennia Sánchez-Pérez, Yolanda I. Chirino. Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production. Toxicology. 2020; 442 ():152545.
Chicago/Turabian StyleAlejandro Déciga-Alcaraz; Estefany I. Medina-Reyes; Norma L. Delgado-Buenrostro; Carolina Rodriguez Ibarra; Adriana Ganem-Rondero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda; Jorge H. Limón-Pacheco; Claudia M. García-Cuéllar; Yesennia Sánchez-Pérez; Yolanda I. Chirino. 2020. "Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production." Toxicology 442, no. : 152545.
Some studies have shown that silicon dioxide nanoparticles (SiO2-NPs) can reach different regions of the brain and cause toxicity; however, the consequences of SiO2-NPs exposure on the diverse brain cell lineages is limited. We aimed to investigate the neurotoxic effects of SiO2-NP (0–100 µg/mL) on rat astrocyte-rich cultures or neuron-rich cultures using scanning electron microscopy, Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR), FTIR microspectroscopy mapping (IQ mapping), and cell viability tests. SiO2-NPs were amorphous particles and aggregated in saline and culture media. Both astrocytes and neurons treated with SiO2-NPs showed alterations in cell morphology and changes in the IR spectral regions corresponding to nucleic acids, proteins, and lipids. The analysis by the second derivative revealed a significant decrease in the signal of the amide I (α-helix, parallel β-strand, and random coil) at the concentration of 10 µg/mL in astrocytes but not in neurons. IQ mapping confirmed changes in nucleic acids, proteins, and lipids in astrocytes; cell death was higher in astrocytes than in neurons (10–100 µg/mL). We conclude that astrocytes were more vulnerable than neurons to SiO2-NPs toxicity. Therefore, the evaluation of human exposure to SiO2-NPs and possible neurotoxic effects must be followed up.
Jorge Humberto Limón-Pacheco; Natalie Jiménez-Barrios; Alejandro Déciga-Alcaraz; Adriana Martínez-Cuazitl; Mónica Maribel Mata-Miranda; Gustavo Jesús Vázquez-Zapién; Jose Pedraza-Chaverri; Yolanda Irasema Chirino; Marisol Orozco-Ibarra. Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro. Toxics 2020, 8, 51 .
AMA StyleJorge Humberto Limón-Pacheco, Natalie Jiménez-Barrios, Alejandro Déciga-Alcaraz, Adriana Martínez-Cuazitl, Mónica Maribel Mata-Miranda, Gustavo Jesús Vázquez-Zapién, Jose Pedraza-Chaverri, Yolanda Irasema Chirino, Marisol Orozco-Ibarra. Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro. Toxics. 2020; 8 (3):51.
Chicago/Turabian StyleJorge Humberto Limón-Pacheco; Natalie Jiménez-Barrios; Alejandro Déciga-Alcaraz; Adriana Martínez-Cuazitl; Mónica Maribel Mata-Miranda; Gustavo Jesús Vázquez-Zapién; Jose Pedraza-Chaverri; Yolanda Irasema Chirino; Marisol Orozco-Ibarra. 2020. "Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro." Toxics 8, no. 3: 51.
La cicatrización de la piel es un proceso complejo y organizado que involucra tres fases: inflamatoria, proliferativa y de remodelación. Es indispensable el análisis de este proceso biomolecularmente para investigar y proponer nuevas estrategias terapéuticas que mejoren la cicatrización o promuevan la regeneración. El objetivo de este proyecto fue analizar histológica y biomolecularmente mediante microespectroscopía infrarroja por transformada de Fourier (MFTIR) y su función de mapeo bioquímico, muestras de lesiones excisionales de piel, comparando los cambios morfológicos y espectroscópicos entre piel sana y piel cicatrizada. Se estandarizó un modelo de lesión excisional de piel en ratones hembra de la cepa NIH de 8 semanas de edad (n=16), provocando una herida excisional de 1 cm2. Se analizó piel sana (día 0) y cicatrizada (día 15 post-lesión) morfométrica, histológica y biomolecularmente mediante análisis fotográfico, técnica histológica y MFTIR con su función de mapeo. El análisis morfométrico demostró una reducción del área de la herida en un 87,6 % al día 15 post-lesión. Histológicamente, en la piel cicatrizada se evidenció un adelgazamiento de la epidermis y menor celularidad en la dermis, observándose la formación de tejido de granulación y fibras de colágena desorganizadas. Espectroscópicamente, se apreciaron cambios entre los dos grupos de estudio, principalmente en las bandas de lípidos y en la región de proteínas. El cálculo de las áreas bajo la curva y el mapeo bioquímico mostraron menor concentración de queratina y colágena en la piel cicatrizada, así como desorganización de las fibras de colágena. Se demostró la capacidad de la MFTIR para caracterizar de forma precisa los cambios biomoleculares en la cicatrización, entre ellos la cantidad de queratina, colágena, y el depósito y ordenamiento de las fibras de colágena asociadas a su maduración.
Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda; Fernando Josué García-Sánchez; Sandy Selene Campos-Soto; Miguel Sánchez-Brito; Adriana Martínez-Cuazitl. Caracterización Biomolecular Mediante Microespectroscopía FTIR en la Fase de Remodelación de la Cicatrización en un Modelo Murino de Lesión Excisional. International Journal of Morphology 2019, 37, 1234 -1244.
AMA StyleGustavo Jesus Vazquez-Zapien, Monica Maribel Mata-Miranda, Fernando Josué García-Sánchez, Sandy Selene Campos-Soto, Miguel Sánchez-Brito, Adriana Martínez-Cuazitl. Caracterización Biomolecular Mediante Microespectroscopía FTIR en la Fase de Remodelación de la Cicatrización en un Modelo Murino de Lesión Excisional. International Journal of Morphology. 2019; 37 (4):1234-1244.
Chicago/Turabian StyleGustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda; Fernando Josué García-Sánchez; Sandy Selene Campos-Soto; Miguel Sánchez-Brito; Adriana Martínez-Cuazitl. 2019. "Caracterización Biomolecular Mediante Microespectroscopía FTIR en la Fase de Remodelación de la Cicatrización en un Modelo Murino de Lesión Excisional." International Journal of Morphology 37, no. 4: 1234-1244.
Fourier Transform Infrared Micro-spectroscopy (FTIRM) is an emerging tool that obtains images with biochemical information of samples that are too small to be chemically analyzed by conventional Fourier transform infrared (FTIR) spectroscopy techniques. So, the central objective of this project was to study the biochemical similarity between articular and cultured chondrocytes by chemometric analysis from FTIRM. Nine samples of knee articular cartilage were obtained; each sample was divided into two fragments, one portion was used for FTIRM characterization in situ, and from another part, chondrocytes were obtained to be cultured (in vitro), which were subjected to an FTIRM to characterize their biomolecular components. The FTIRM spectra were normalized, and the second derivative was calculated. From these data, principal component analysis (PCA) and a chemometric comparison between in situ and cultured chondrocytes were carried out. Finally, the biochemical mapping was conducted obtaining micro-FTIR imaging. FTIRM spectra of in situ and in vitro chondrocytes were obtained, and different biomolecules were detected, highlighting lipids, proteins, glycosaminoglycans, collagen, and aggrecan. Despite slight differences in the FTIR spectra, the PCA proved the organic similarity between in situ chondrocytes and cultured chondrocytes, which was also observed in the analysis of the ratios related to the degradation of the articular cartilage and collagen. In the same way, the ability of the FTIRM to characterize the molecular biodistribution was demonstrated. The biochemical composition and biodistribution analysis using FTIRM have been useful for comparing cultured chondrocytes and in situ chondrocytes.
Monica Maribel Mata-Miranda; Adriana Martinez-Cuazitl; Carla Ivonne Guerrero Robles; Jesus Emmanuel Noriega-Gonzalez; Juan Salvador Garcia-Hernandez; Gustavo Jesus Vazquez-Zapien. Biochemical similarity between cultured chondrocytes and in situ chondrocytes by chemometric analysis from FTIR microspectroscopy. Biotechnology Reports 2019, 24, e00391 .
AMA StyleMonica Maribel Mata-Miranda, Adriana Martinez-Cuazitl, Carla Ivonne Guerrero Robles, Jesus Emmanuel Noriega-Gonzalez, Juan Salvador Garcia-Hernandez, Gustavo Jesus Vazquez-Zapien. Biochemical similarity between cultured chondrocytes and in situ chondrocytes by chemometric analysis from FTIR microspectroscopy. Biotechnology Reports. 2019; 24 ():e00391.
Chicago/Turabian StyleMonica Maribel Mata-Miranda; Adriana Martinez-Cuazitl; Carla Ivonne Guerrero Robles; Jesus Emmanuel Noriega-Gonzalez; Juan Salvador Garcia-Hernandez; Gustavo Jesus Vazquez-Zapien. 2019. "Biochemical similarity between cultured chondrocytes and in situ chondrocytes by chemometric analysis from FTIR microspectroscopy." Biotechnology Reports 24, no. : e00391.
Introduction. The stress fractures (SFs) are a common condition in athletes and military recruits, characterized by partial fracture caused by repetitive applications of stresses that are lower than the stress required to fracture the bone in a single loading. Fourier transform infrared (FTIR) spectroscopy gives information about the bone composition and also can determine the amount of a molecule. For this reason, the FTIR spectroscopy may be used as a tool for diagnosis of certain bone diseases related to the bone strength. In this research, we established the contributions of mineral and collagen properties to SF risk through FTIR spectroscopy, analyzing the biochemical profile differences between the healthy bone and the bone with an SF. Materials and Methods. Previous written informed consent was obtained, and samples of the hip with an SF (n = 11) and healthy bone from the femur with traumatic fracture (n = 5) were obtained and analyzed employing FTIR spectroscopy and its biochemical mapping function. Then, using FTIR spectra and the map, the collagen content and ratios corresponding to matrix maturity, mineralization, carbonate substitution, acid phosphate substitution, and crystallinity were calculated. Moreover, a histopathological analysis through Masson’s staining was conducted. Results. The biochemical analysis showed that the bone with an SF presented a bone immaturity characterized by a higher content of collagen, lower matrix maturity, mineralization, carbonate and acid phosphate substitutions, and greater crystallinity compared to the healthy bone, being checked by the ratio analysis and biochemical mapping. Besides, Masson’s stain showed a higher collagen content in the bone with an SF. Conclusions. The bone with an SF presented alterations in its biochemical composition, showing bone immaturity, which broadens the panorama of the condition to investigate future treatments or prophylactic techniques.
Monica Maribel Mata-Miranda; Melissa Guerrero-Ruiz; Juan Ramon Gonzalez-Fuentes; Carlos Martin Hernandez-Toscano; Jesus Rafael Garcia-Andino; Miguel Sanchez-Brito; Gustavo Jesus Vazquez-Zapien. Characterization of the Biological Fingerprint and Identification of Associated Parameters in Stress Fractures by FTIR Spectroscopy. BioMed Research International 2019, 2019, 1 -10.
AMA StyleMonica Maribel Mata-Miranda, Melissa Guerrero-Ruiz, Juan Ramon Gonzalez-Fuentes, Carlos Martin Hernandez-Toscano, Jesus Rafael Garcia-Andino, Miguel Sanchez-Brito, Gustavo Jesus Vazquez-Zapien. Characterization of the Biological Fingerprint and Identification of Associated Parameters in Stress Fractures by FTIR Spectroscopy. BioMed Research International. 2019; 2019 ():1-10.
Chicago/Turabian StyleMonica Maribel Mata-Miranda; Melissa Guerrero-Ruiz; Juan Ramon Gonzalez-Fuentes; Carlos Martin Hernandez-Toscano; Jesus Rafael Garcia-Andino; Miguel Sanchez-Brito; Gustavo Jesus Vazquez-Zapien. 2019. "Characterization of the Biological Fingerprint and Identification of Associated Parameters in Stress Fractures by FTIR Spectroscopy." BioMed Research International 2019, no. : 1-10.
Cisplatin (cis-diaminodichloroplatin (II), CDDP) is an antineoplastic drug used in the treatment of many types of cancer of solid organs. Although CDDP side effects include ototoxicity, gastric toxicity, myelosuppression and allergic reactions, the main limitation of this antineoplastic agent is nephrotoxicity, provoking acute kidney failure (AKI). The pathophysiology of AKI induced by CDDP is complex, there are several mechanisms involved, including nuclear and mitochondrial damage, activation of apoptosis signaling pathways, generation of reactive oxygen species, and inflammation. In this review, we describe the molecular events of CDDP, which cause nephrotoxicity, affecting the expression of several genes involved in metabolism and activating different apoptotic signaling pathways, highlighting the main cellular targets of this antineoplastic and the approach of possible treatments for AKI caused by CDDP. El cisplatino (cis-diaminodicloroplatino(II), CDDP) es un fármaco antineoplásico usado en el tratamiento de muchos tipos de cáncer en órganos sólidos. Aunque los daños por CDDP incluyen ototoxicidad, gastrotoxicidad, mielosupresión y reacciones alérgicas, la nefrotoxicidad es el principal efecto limitante de este antineoplásico, provocando comúnmente insuficiencia renal aguda (IRA). La fisiopatología de la IRA inducida por CDDP es compleja, ya que están involucrados diversos mecanismos como el daño nuclear y mitocondrial, la activación de vías de señalización de apoptosis, generación de especies reactivas de oxígeno y la estimulación de la inflamación. En esta revisión, se describen los eventos moleculares por los cuales el CDDP tiene su acción nefrotóxica al alterar la expresión de diversos genes que participan en el metabolismo y al activar diferentes vías de señalización apoptóticas, resaltando las principales dianas celulares de este antineoplásico y su uso para el planteamiento de posibles tratamientos para la IRA causada por CDDP.
Carlos Eduardo Bernal-Barquero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda. Review of alterations in gene expression and apoptotic pathways caused in nephrotoxicity induced by cisplatin. Nefrología (English Edition) 2019, 39, 362 -371.
AMA StyleCarlos Eduardo Bernal-Barquero, Gustavo Jesus Vazquez-Zapien, Monica Maribel Mata-Miranda. Review of alterations in gene expression and apoptotic pathways caused in nephrotoxicity induced by cisplatin. Nefrología (English Edition). 2019; 39 (4):362-371.
Chicago/Turabian StyleCarlos Eduardo Bernal-Barquero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda. 2019. "Review of alterations in gene expression and apoptotic pathways caused in nephrotoxicity induced by cisplatin." Nefrología (English Edition) 39, no. 4: 362-371.
Breast cancer (BC) is a malignant disease with a high prevalence worldwide. The main cause of death is not the primary tumor, but instead the spread of tumor cells to distant sites. The aim of the present study was to examine a new method for the detection of cancer cells in aqueous medium using bioimpedance spectroscopy assisted with magnetic nanoparticles (MNP’s) exposure to a constant magnetic field. The spectroscopic patterns were identified for three breast cancer cell lines. Each BC cell line represents a different pathologic stage: the early stage (MCF-7), invasive phase (MDA-MB-231) and metastasis (SK-BR-3). For this purpose, bioimpedance measurements were carried out at a certain frequency range with the aid of nanoprobes, consisting of magnetic nanoparticles (MNPs) coupled to a monoclonal antibody. The antibody was specific for the predominant cell surface protein for each cell line, which was identified by using RT-qPCR and flow cytometry. Accordingly, EpCAM corresponds to MCF-7, MUC-1 to MDA-MB-231, and HER-2 to SK-BR-3. Despite their low concentrations, BC cells could be detected by impedance spectroscopy. Hence, this methodology should permit the monitoring of circulating tumor cells (CTC) and therefore help to prevent recurrences and metastatic processes during BC treatment.
L. F. E. Huerta-Nuñez; G. Gutierrez-Iglesias; Adriana Martínez-Cuazitl; Monica Maribel Mata-Miranda; V. D. Alvarez-Jiménez; V. Sánchez-Monroy; Alexander Golberg; C. A. González-Díaz. A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy. Scientific Reports 2019, 9, 1 -12.
AMA StyleL. F. E. Huerta-Nuñez, G. Gutierrez-Iglesias, Adriana Martínez-Cuazitl, Monica Maribel Mata-Miranda, V. D. Alvarez-Jiménez, V. Sánchez-Monroy, Alexander Golberg, C. A. González-Díaz. A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy. Scientific Reports. 2019; 9 (1):1-12.
Chicago/Turabian StyleL. F. E. Huerta-Nuñez; G. Gutierrez-Iglesias; Adriana Martínez-Cuazitl; Monica Maribel Mata-Miranda; V. D. Alvarez-Jiménez; V. Sánchez-Monroy; Alexander Golberg; C. A. González-Díaz. 2019. "A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy." Scientific Reports 9, no. 1: 1-12.
Carlos Eduardo Bernal-Barquero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda. Revisión de las alteraciones en la expresión génica y vías apoptóticas provocadas en la nefrotoxicidad inducida por cisplatino. Nefrología 2019, 39, 362 -371.
AMA StyleCarlos Eduardo Bernal-Barquero, Gustavo Jesus Vazquez-Zapien, Monica Maribel Mata-Miranda. Revisión de las alteraciones en la expresión génica y vías apoptóticas provocadas en la nefrotoxicidad inducida por cisplatino. Nefrología. 2019; 39 (4):362-371.
Chicago/Turabian StyleCarlos Eduardo Bernal-Barquero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda. 2019. "Revisión de las alteraciones en la expresión génica y vías apoptóticas provocadas en la nefrotoxicidad inducida por cisplatino." Nefrología 39, no. 4: 362-371.
Introduction. The acute kidney injury (AKI) is characterized by a sudden glomerular filtration reduction. Renal or intrinsic causes of AKI include nephrotoxicity induced by exogenous agents like cisplatin, which causes oxidative stress altering the biochemical process and leading to apoptosis. Therefore, this research is aimed at analyzing the embryonic stem cells (ESC) nephroprotective effect in AKI induced by cisplatin, employing genetic, phenotypic, and microspectroscopic techniques. Methods. Thirty mice were randomly divided into three groups ( n = 10 ): the healthy, isotonic salt solution (ISS), and mouse embryonic stem cells (mESC) groups. The ISS and mESC groups were subjected to AKI using cisplatin; 24 h post-AKI received an intraperitoneal injection of ISS or 1 × 10 6 mESC, respectively. At days 4 and 8 post-AKI, five mice of each group were sacrificed to analyze the histopathological, genetic (PDK4 and HO-1), protein (p53), and vibrational microspectroscopic changes. Results. Histopathologically, interstitial nephritis and acute tubular necrosis were observed; however, the mESC group showed a more preserved microarchitecture with high cellularity. Additionally, the PDK4 and HO-1 gene expression only increased in the ISS group on day 4 post-AKI. Likewise, p53 was more immunoexpressed at day 8 post-AKI in the ISS group. About biomolecular analysis by microspectroscopy, bands associated with lipids, proteins, and nucleic acids were evidenced. Besides, ratios related to membrane function (protein/lipid), unsaturated lipid content (olefinic/total lipid, olefinic/total CH2, and CH2/CH3), and lipid peroxidation demonstrated oxidative stress induction and lipid peroxidation increase mainly in the ISS group. Finally, the principal component analysis discriminated against each group; nonetheless, some data of the healthy and mESC groups at day 8 were correlated. Conclusions. The mESC implant diminishes cisplatin nephrotoxicity, once the protective effect in the reduction of lipid peroxidation was demonstrated, reflecting a functional and histological restoration.
Monica Maribel Mata-Miranda; Carlos Eduardo Bernal-Barquero; Adriana Martínez-Cuazitl; Carla Ivonne Guerrero-Robles; Virginia Sanchez-Monroy; Marlon Rojas-Lopez; Gustavo Jesus Vazquez-Zapien. Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin. Oxidative Medicine and Cellular Longevity 2019, 2019, 1 -14.
AMA StyleMonica Maribel Mata-Miranda, Carlos Eduardo Bernal-Barquero, Adriana Martínez-Cuazitl, Carla Ivonne Guerrero-Robles, Virginia Sanchez-Monroy, Marlon Rojas-Lopez, Gustavo Jesus Vazquez-Zapien. Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin. Oxidative Medicine and Cellular Longevity. 2019; 2019 ():1-14.
Chicago/Turabian StyleMonica Maribel Mata-Miranda; Carlos Eduardo Bernal-Barquero; Adriana Martínez-Cuazitl; Carla Ivonne Guerrero-Robles; Virginia Sanchez-Monroy; Marlon Rojas-Lopez; Gustavo Jesus Vazquez-Zapien. 2019. "Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin." Oxidative Medicine and Cellular Longevity 2019, no. : 1-14.
We evaluate structural damage effects of heat on DNA obtained from the dental pulp of restored premolars. We studied three groups (A, B and C) each group comprised twenty premolars extracted from five patients. Three of the four premolars of each donator were restored with different materials: amalgam, glass ionomer and resin, and one unrestored premolar was used as control. The group A was not exposed to heat, while B and C groups were exposed to 100°C and 300°C, respectively. The DNA damage was evaluated as percentage of genotyping of 15 Short Tandem Repeats (STRs) and amelogenin and by Fourier-transform infrared spectroscopy (FTIR). The results showed 100% genotyping in all unheated premolars; however, the increase in heat decreased genotyping percentage due to DNA degradation. The amplifications from the premolars restored with glass ionomer and those unrestored were less affected, amplifying by approximately 80% at 300°C. FTIR revealed that DNA structural damage occurred in the phosphate region, and changes in ribose were also shown; in addition, we detected presence of β- three-calcium-phosphate (β - TCP) due to heat treatment. Moreover, the phosphate region of DNA was a good indicator of DNA integrity related to the ratio of 1230/1085 cm-1 in the second derivative (asymmetric/symmetric PO2), which was major in premolars restored with glass ionomer heated at 100°C, and this ratio is related to less DNA alterations and better genotyping; however this changes only were detected at 100°C, suggesting that dental restoration with this material only protects dental pulp at temperatures below 300°C.
Lucía Angeles Estrada; Adriana Martínez-Cuazitl; Mónica Maribel Mata-Miranda; César Antonio González-Díaz; Gustavo Jesús Vázquez-Zapién; Elsa Saldaña Rivera; Virginia Sánchez-Monroy; Adriana Martínez-Cuatzitl. Evaluation of the effect of heat damage on DNA extracted from the dental pulp of restored teeth. Legal Medicine 2019, 37, 54 -59.
AMA StyleLucía Angeles Estrada, Adriana Martínez-Cuazitl, Mónica Maribel Mata-Miranda, César Antonio González-Díaz, Gustavo Jesús Vázquez-Zapién, Elsa Saldaña Rivera, Virginia Sánchez-Monroy, Adriana Martínez-Cuatzitl. Evaluation of the effect of heat damage on DNA extracted from the dental pulp of restored teeth. Legal Medicine. 2019; 37 ():54-59.
Chicago/Turabian StyleLucía Angeles Estrada; Adriana Martínez-Cuazitl; Mónica Maribel Mata-Miranda; César Antonio González-Díaz; Gustavo Jesús Vázquez-Zapién; Elsa Saldaña Rivera; Virginia Sánchez-Monroy; Adriana Martínez-Cuatzitl. 2019. "Evaluation of the effect of heat damage on DNA extracted from the dental pulp of restored teeth." Legal Medicine 37, no. : 54-59.
Gustavo Vazquez-Zapien; Adriana Martínez-Cuazitl; Laura Susana Rangel-Cova; Alejandro Camacho-Ibarra; Monica Mata-Miranda. Biochemical and histological effects of embryonic stem cells in a mouse model of renal failure. 2019, 60, 189 -194.
AMA StyleGustavo Vazquez-Zapien, Adriana Martínez-Cuazitl, Laura Susana Rangel-Cova, Alejandro Camacho-Ibarra, Monica Mata-Miranda. Biochemical and histological effects of embryonic stem cells in a mouse model of renal failure. . 2019; 60 (1):189-194.
Chicago/Turabian StyleGustavo Vazquez-Zapien; Adriana Martínez-Cuazitl; Laura Susana Rangel-Cova; Alejandro Camacho-Ibarra; Monica Mata-Miranda. 2019. "Biochemical and histological effects of embryonic stem cells in a mouse model of renal failure." 60, no. 1: 189-194.
Fecal incontinence is a disabling condition with devastating consequences for the patients. Medical and surgical options are not very satisfactory, reason by which regenerative medicine has been considered in this field. In the present research, we analyzed functional and histologic effects after implanting pluripotent stem cells (PSCs) in a murine model with sphincterotomy. Female Wistar rats were subjected to sphincterotomy and divided into three groups. Group 1 (control group) was treated with 300 μL of balanced saline solution and group 2 (late treatment) and group 3 (early treatment) received 50,000 PSCs resuspended in 300 μL of balanced saline solution. All animals were evaluated through high-resolution anorectal manometry 24 hours before and after sphincterotomy and every month for three months. Finally, the rats were euthanized and histopathologic sections from the anal canal were obtained. All groups showed a decrease in resting anal pressure and squeeze anal pressure 24 hours after sphincterotomy. At the third month, higher anal pressures in the groups treated with PSCs were detected. Regarding the histologic effects, the microscopic architecture was restored and there was a significant decrease in the inflammatory response in the groups treated with PSCs. PSCs implantation improves anal tone, as well as histologic structure, presenting better regenerative results when implanted as early treatment. La incontinencia fecal es una condición incapacitante con consecuencias devastadoras para los pacientes. Las opciones médicas y quirúrgicas no son muy satisfactorias, razón por la cual la medicina regenerativa ha sido considerada en este campo. En esta investigación se analizaron los efectos funcionales e histológicos posteriores al implante de células madre pluripotentes (CMP) en un modelo murino con esfinterotomía. Ratas hembra Wistar fueron sometidas a esfinterotomía y divididas en tres grupos. El grupo 1 (grupo control) fue tratado con 300 μl de solución salina isotónica, el grupo 2 (tratamiento tardío) y el grupo 3 (tratamiento temprano) recibieron 50,000 CMP resuspendidas en 300 μl de solución salina isotónica. Todos los animales fueron evaluados por medio de manometría anorrectal de alta resolución 24 h previo y posterior a la esfinterotomía y cada mes durante tres meses. Finalmente, las ratas fueron sacrificadas y se obtuvieron cortes histopatológicos del canal anal. Todos los grupos mostraron una disminución en las presiones anales de reposo y esfuerzo 24 h después de la esfinterotomía. Al tercer mes, se obtuvieron presiones anales más elevadas en los grupos tratados con CMP, observando una diferencia estadísticamente significativa en la presión anal de esfuerzo. En cuanto a los efectos histológicos, se observó restauración de la arquitectura microscópica, así como una disminución significativa de la respuesta inflamatoria en los grupos tratados con CMP. El implante de CMP mejora el tono anal y...
G.J. Vázquez-Zapién; M.E. Ordoñez-Gutiérrez; J.I. Minero-Alfaro; V.H. Guerrero-Guerrero; I. Mora-Mendoza; M.M. Mata-Miranda. Functional and histologic effects after implanting pluripotent stem cells in a murine model with sphincterotomy. Revista de Gastroenterología de México (English Edition) 2018, 84, 165 -173.
AMA StyleG.J. Vázquez-Zapién, M.E. Ordoñez-Gutiérrez, J.I. Minero-Alfaro, V.H. Guerrero-Guerrero, I. Mora-Mendoza, M.M. Mata-Miranda. Functional and histologic effects after implanting pluripotent stem cells in a murine model with sphincterotomy. Revista de Gastroenterología de México (English Edition). 2018; 84 (2):165-173.
Chicago/Turabian StyleG.J. Vázquez-Zapién; M.E. Ordoñez-Gutiérrez; J.I. Minero-Alfaro; V.H. Guerrero-Guerrero; I. Mora-Mendoza; M.M. Mata-Miranda. 2018. "Functional and histologic effects after implanting pluripotent stem cells in a murine model with sphincterotomy." Revista de Gastroenterología de México (English Edition) 84, no. 2: 165-173.
Gustavo Vázquez-Zapién; Mónica Mata-Miranda; Raúl Delgado-Macuil; Marlon Rojas-López; Rosbelia Ramos-Roldan; Olga Aguilar; Virginia Sanchez-Monroy. Genetic, Protein and FTIR Spectroscopic Comparison of Anterior and Posterior Deciduous Dental Pulp for Subsequent Obtention of SHED. Annual Research & Review in Biology 2018, 23, 1 -11.
AMA StyleGustavo Vázquez-Zapién, Mónica Mata-Miranda, Raúl Delgado-Macuil, Marlon Rojas-López, Rosbelia Ramos-Roldan, Olga Aguilar, Virginia Sanchez-Monroy. Genetic, Protein and FTIR Spectroscopic Comparison of Anterior and Posterior Deciduous Dental Pulp for Subsequent Obtention of SHED. Annual Research & Review in Biology. 2018; 23 (4):1-11.
Chicago/Turabian StyleGustavo Vázquez-Zapién; Mónica Mata-Miranda; Raúl Delgado-Macuil; Marlon Rojas-López; Rosbelia Ramos-Roldan; Olga Aguilar; Virginia Sanchez-Monroy. 2018. "Genetic, Protein and FTIR Spectroscopic Comparison of Anterior and Posterior Deciduous Dental Pulp for Subsequent Obtention of SHED." Annual Research & Review in Biology 23, no. 4: 1-11.
The identification and characterization of diverse cells types and cell differentiation process requires complex techniques as flow cytometry, immunocytochemistry and the exploration of molecular markers; such techniques require infrastructure and qualified personnel. The objective of this study was to analyze the use of Electrical Bioimpedance Spectroscopy (EBIS) measurements as a non-complex alternative technique to identify populations of undifferentiated mouse Pluripotent Stem Cells (mPSCs), Mouse Embryonic Fibroblasts (MEFs) and the differentiation process from preadipocytes (3T3-L1) to mature adipocytes. EBIS measurements were compared in populations of cells which were characterized previously using microscopy. The results indicate that EBIS technique has a potential sensitivity at certain frequency range to discriminate between both evaluated cell populations and some differentiation process. Additional studies with different concentrations to evaluate quantitatively the sensitivity and specificity of the proposed technique are recommended.
Carla Ivonne Guerrero Robles; G.J. Vazquez-Zapien; M.M. Mata-Miranda; J.E. Noriega-Gonzalez; C.A. Gonzalez-Diaz. Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) 2017, 3568 -3571.
AMA StyleCarla Ivonne Guerrero Robles, G.J. Vazquez-Zapien, M.M. Mata-Miranda, J.E. Noriega-Gonzalez, C.A. Gonzalez-Diaz. Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process. 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). 2017; ():3568-3571.
Chicago/Turabian StyleCarla Ivonne Guerrero Robles; G.J. Vazquez-Zapien; M.M. Mata-Miranda; J.E. Noriega-Gonzalez; C.A. Gonzalez-Diaz. 2017. "Electrical bioimpedance spectroscopy as biosensor technique to identify cells lineages and cell differentiation process." 2017 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) , no. : 3568-3571.
Kidney diseases are a public health problem worldwide. Available therapies include function replacement by dialysis or transplant, which are associated with a high morbidity and mortality. Likewise, none of these treatments compensate all kidney functions. There is a great concern in developing more effective therapies with the ability to replace the wide range of renal functions, so that, new studies on developing therapeutic strategies have focused on regenerative medicine. The aim of this paper is to review the new advances in regenerative medicine for renal failure treatment. Regenerative medicine comprises two therapeutic strategies: cell therapy and tissue engineering. Cell therapy techniques depend on cell and tissue cultures, with the aim to replace morphological structures, tissues, and functions. The main strategic strength of cell therapy in renal failure is the incorporation of additional cells in a damaged kidney, for which purpose different kind of Stem Cells (SCs) have been used such as Embryonic SCs, induced Pluripotent SCs, Multipotent SCs, Renal SCs, or drugs that increase survival and mobilization of SCs. Tissue engineering complements cell therapy combining techniques of biological sciences and engineering to create structures and devices as scaffolds, matrices or 3D biocompatible materials. Even though there is a significant advance in regenerative medicine strategies, we are far from using any of its techniques on health institutions, due to it is necessary to evaluate side effects, biodistribution, dosage, type of administration, vehicle of cell therapy, as well as the evaluation of response time and long-term studies, among other studies.
Monica Maribel Mata-Miranda; Raul Jacobo Delgado-Macuil; Marlon Rojas-Lopez; Ricardo Martinez-Flores; Gustavo Jesus Vazquez-Zapien. Potential Therapeutic Strategies of Regenerative Medicine for Renal Failure. Current Stem Cell Research & Therapy 2017, 12, 423 -431.
AMA StyleMonica Maribel Mata-Miranda, Raul Jacobo Delgado-Macuil, Marlon Rojas-Lopez, Ricardo Martinez-Flores, Gustavo Jesus Vazquez-Zapien. Potential Therapeutic Strategies of Regenerative Medicine for Renal Failure. Current Stem Cell Research & Therapy. 2017; 12 (5):423-431.
Chicago/Turabian StyleMonica Maribel Mata-Miranda; Raul Jacobo Delgado-Macuil; Marlon Rojas-Lopez; Ricardo Martinez-Flores; Gustavo Jesus Vazquez-Zapien. 2017. "Potential Therapeutic Strategies of Regenerative Medicine for Renal Failure." Current Stem Cell Research & Therapy 12, no. 5: 423-431.