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Yolanda I. Chirino
Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No. 1, Tlalnepantla de Baz, CP, 54090, Estado de México, Mexico

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Journal article
Published: 28 July 2021 in Chemico-Biological Interactions
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Inhaled nanoparticles (NPs) challenges mobile and immobile barriers in the respiratory tract, which can be represented by type II pneumocytes (immobile) and monocytes (mobile) but what is more important for biological effects, the cell linage, or the type of nanoparticle? Here, we addressed these questions and we demonstrated that the type of NPs exerts a higher influence on biological effects, but cell linages also respond differently against similar type of NPs. Design. Type II pneumocytes and monocytes were exposed to tin dioxide (SnO2) NPs and titanium dioxide (TiO2) NPs (1, 10 and 50 μg/cm2) for 24 h and cell viability, ultrastructure, cell granularity, molecular spectra of lipids, proteins and nucleic acids and cytoskeleton architecture were evaluated. Results. SnO2 NPs and TiO2 NPs are metal oxides with similar physicochemical properties. However, in the absence of cytotoxicity, SnO2 NPs uptake was low in monocytes and higher in type II pneumocytes, while TiO2 NPs were highly internalized by both types of cells. Monocytes exposed to both types of NPs displayed higher number of alterations in the molecular patterns of proteins and nuclei acids analyzed by Fourier-transform infrared spectroscopy (FTIR) than type II pneumocytes. In addition, cells exposed to TiO2 NPs showed more displacements in FTIR spectra of biomolecules than cells exposed to SnO2 NPs. Regarding cell architecture, microtubules were stable in type II pneumocytes exposed to both types of NPs but actin filaments displayed a higher number of alterations in type II pneumocytes and monocytes exposed to SnO2 NPs and TiO2 NPs. NPs exposure induced the formation of large vacuoles only in monocytes, which were not seen in type II pneumocytes. Conclusions. Most of the cellular effects are influenced by the NPs exposure rather than by the cell type. However, mobile, and immobile barriers in the respiratory tract displayed differential response against SnO2 NPs and TiO2 NPs in absence of cytotoxicity, in which monocytes were more susceptible than type II pneumocytes to NPs exposure.

ACS Style

Octavio Ispanixtlahuatl-Meráz; Norma L. Delgado-Buenrostro; Alejandro Déciga-Alcaraz; María Del Pilar Ramos-Godinez; Diego Oliva-Rico; Edgar O. López-Villegas; Gustavo J. Vázquez-Zapién; Mónica M. Mata-Miranda; Damaris Ilhuicatzi-Alvarado; Leticia Moreno-Fierros; Claudia M. García Cuellar; Yesennia Sánchez-Pérez; Yolanda I. Chirino. Differential response of immobile (pneumocytes) and mobile (monocytes) barriers against 2 types of metal oxide nanoparticles. Chemico-Biological Interactions 2021, 347, 109596 .

AMA Style

Octavio Ispanixtlahuatl-Meráz, Norma L. Delgado-Buenrostro, Alejandro Déciga-Alcaraz, María Del Pilar Ramos-Godinez, Diego Oliva-Rico, Edgar O. López-Villegas, Gustavo J. Vázquez-Zapién, Mónica M. Mata-Miranda, Damaris Ilhuicatzi-Alvarado, Leticia Moreno-Fierros, Claudia M. García Cuellar, Yesennia Sánchez-Pérez, Yolanda I. Chirino. Differential response of immobile (pneumocytes) and mobile (monocytes) barriers against 2 types of metal oxide nanoparticles. Chemico-Biological Interactions. 2021; 347 ():109596.

Chicago/Turabian Style

Octavio Ispanixtlahuatl-Meráz; Norma L. Delgado-Buenrostro; Alejandro Déciga-Alcaraz; María Del Pilar Ramos-Godinez; Diego Oliva-Rico; Edgar O. López-Villegas; Gustavo J. Vázquez-Zapién; Mónica M. Mata-Miranda; Damaris Ilhuicatzi-Alvarado; Leticia Moreno-Fierros; Claudia M. García Cuellar; Yesennia Sánchez-Pérez; Yolanda I. Chirino. 2021. "Differential response of immobile (pneumocytes) and mobile (monocytes) barriers against 2 types of metal oxide nanoparticles." Chemico-Biological Interactions 347, no. : 109596.

Journal article
Published: 19 July 2021 in International Journal of Molecular Sciences
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Signal transducer and activator of transcription 1 (STAT1) acts as a tumor suppressor molecule in colitis-associated colorectal cancer (CAC), particularly during the very early stages, modulating immune responses and controlling mechanisms such as apoptosis and cell proliferation. Previously, using an experimental model of CAC, we reported increased intestinal cell proliferation and faster tumor development, which were consistent with more signs of disease and damage, and reduced survival in STAT1-/- mice, compared with WT counterparts. However, the mechanisms through which STAT1 might prevent colorectal cancer progression preceded by chronic inflammation are still unclear. Here, we demonstrate that increased tumorigenicity related to STAT1 deficiency could be suppressed by IL-17 neutralization. The blockade of IL-17 in STAT1-/- mice reduced the accumulation of CD11b+Ly6ClowLy6G+ cells resembling granulocytic myeloid-derived suppressor cells (MDSCs) in both spleen and circulation. Additionally, IL-17 blockade reduced the recruitment of neutrophils into intestinal tissue, the expression and production of inflammatory cytokines, and the expression of intestinal STAT3. In addition, the anti-IL-17 treatment also reduced the expression of Arginase-1 and inducible nitric oxide synthase (iNOS) in the colon, both associated with the main suppressive activity of MDSCs. Thus, a lack of STAT1 signaling induces a significant change in the colonic microenvironment that supports inflammation and tumor formation. Anti-IL-17 treatment throughout the initial stages of CAC related to STAT1 deficiency abrogates the tumor formation possibly caused by myeloid cells.

ACS Style

Yael Delgado-Ramirez; Itzel Baltazar-Perez; Yamileth Martinez; Blanca Callejas; Itzel Medina-Andrade; Jonadab Olguín; Norma Delgado-Buenrostro; Yolanda Chirino; Luis Terrazas; Sonia Leon-Cabrera. STAT1 Is Required for Decreasing Accumulation of Granulocytic Cells via IL-17 during Initial Steps of Colitis-Associated Cancer. International Journal of Molecular Sciences 2021, 22, 7695 .

AMA Style

Yael Delgado-Ramirez, Itzel Baltazar-Perez, Yamileth Martinez, Blanca Callejas, Itzel Medina-Andrade, Jonadab Olguín, Norma Delgado-Buenrostro, Yolanda Chirino, Luis Terrazas, Sonia Leon-Cabrera. STAT1 Is Required for Decreasing Accumulation of Granulocytic Cells via IL-17 during Initial Steps of Colitis-Associated Cancer. International Journal of Molecular Sciences. 2021; 22 (14):7695.

Chicago/Turabian Style

Yael Delgado-Ramirez; Itzel Baltazar-Perez; Yamileth Martinez; Blanca Callejas; Itzel Medina-Andrade; Jonadab Olguín; Norma Delgado-Buenrostro; Yolanda Chirino; Luis Terrazas; Sonia Leon-Cabrera. 2021. "STAT1 Is Required for Decreasing Accumulation of Granulocytic Cells via IL-17 during Initial Steps of Colitis-Associated Cancer." International Journal of Molecular Sciences 22, no. 14: 7695.

Review
Published: 28 December 2020 in International Journal of Molecular Sciences
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Titanium dioxide (TiO2) is used as a food additive (E171) and can be found in sauces, icings, and chewing gums, as well as in personal care products such as toothpaste and pharmaceutical tablets. Along with the ubiquitous presence of TiO2 and recent insights into its potentially hazardous properties, there are concerns about its application in commercially available products. Especially the nano-sized particle fraction (

ACS Style

Nicolaj S. Bischoff; Theo M. De Kok; Dick T.H.M. Sijm; Simone G. Van Breda; Jacco J. Briedé; Jacqueline J.M. Castenmiller; Antoon Opperhuizen; Yolanda I. Chirino; Hubert Dirven; David Gott; Eric Houdeau; Agnes G. Oomen; Morten Poulsen; Gerhard Rogler; Henk Van Loveren. Possible Adverse Effects of Food Additive E171 (Titanium Dioxide) Related to Particle Specific Human Toxicity, Including the Immune System. International Journal of Molecular Sciences 2020, 22, 207 .

AMA Style

Nicolaj S. Bischoff, Theo M. De Kok, Dick T.H.M. Sijm, Simone G. Van Breda, Jacco J. Briedé, Jacqueline J.M. Castenmiller, Antoon Opperhuizen, Yolanda I. Chirino, Hubert Dirven, David Gott, Eric Houdeau, Agnes G. Oomen, Morten Poulsen, Gerhard Rogler, Henk Van Loveren. Possible Adverse Effects of Food Additive E171 (Titanium Dioxide) Related to Particle Specific Human Toxicity, Including the Immune System. International Journal of Molecular Sciences. 2020; 22 (1):207.

Chicago/Turabian Style

Nicolaj S. Bischoff; Theo M. De Kok; Dick T.H.M. Sijm; Simone G. Van Breda; Jacco J. Briedé; Jacqueline J.M. Castenmiller; Antoon Opperhuizen; Yolanda I. Chirino; Hubert Dirven; David Gott; Eric Houdeau; Agnes G. Oomen; Morten Poulsen; Gerhard Rogler; Henk Van Loveren. 2020. "Possible Adverse Effects of Food Additive E171 (Titanium Dioxide) Related to Particle Specific Human Toxicity, Including the Immune System." International Journal of Molecular Sciences 22, no. 1: 207.

Journal article
Published: 19 November 2020 in Genes
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Triple-negative breast cancer (TNBC) presents a marked diversity at the molecular level, which promotes a clinical heterogeneity that further complicates treatment. We performed a detailed whole exome sequencing profile of 29 Mexican patients with long follow-up TNBC to identify genomic alterations associated with overall survival (OS), disease-free survival (DFS), and pathologic complete response (PCR), with the aim to define their role as molecular predictive factors of treatment response and prognosis. We detected 31 driver genes with pathogenic mutations in TP53 (53%), BRCA1/2 (27%), CDKN1B (9%), PIK3CA (9%), and PTEN (9%), and 16 operative mutational signatures. Moreover, tumors with mutations in BRCA1/2 showed a trend of sensitivity to platinum salts. We found an association between deficiency in DNA repair and surveillance genes and DFS. Across all analyzed tumors we consistently found a heterogeneous molecular complexity in terms of allelic composition and operative mutational processes, which hampered the definition of molecular traits with clinical utility. This work contributes to the elucidation of the global molecular alterations of TNBC by providing accurate genomic data that may help forthcoming studies to improve treatment and survival. This is the first study that integrates genomic alterations with a long follow-up of clinical variables in a Latin American population that is an underrepresented ethnicity in most of the genomic studies.

ACS Style

Ernesto Rojas-Jiménez; Javier César Mejía-Gómez; Clara Díaz-Velásquez; Rosalía Quezada-Urban; Héctor Martínez Gregorio; Fernando Vallejo-Lecuona; Aldo De La Cruz-Montoya; Fany Iris Porras Reyes; Víctor Manuel Pérez-Sánchez; Héctor Aquiles Maldonado-Martínez; Maybelline Robles-Estrada; Enrique Bargalló-Rocha; Paula Cabrera-Galeana; Maritza Ramos-Ramírez; Yolanda Irasema Chirino; Luis Alonso Herrera; Luis Ignacio Terrazas; Javier Oliver; Cecilia Frecha; Sandra Perdomo; Felipe Vaca-Paniagua. Comprehensive Genomic Profile of Heterogeneous Long Follow-Up Triple-Negative Breast Cancer and Its Clinical Characteristics Shows DNA Repair Deficiency Has Better Prognostic. Genes 2020, 11, 1367 .

AMA Style

Ernesto Rojas-Jiménez, Javier César Mejía-Gómez, Clara Díaz-Velásquez, Rosalía Quezada-Urban, Héctor Martínez Gregorio, Fernando Vallejo-Lecuona, Aldo De La Cruz-Montoya, Fany Iris Porras Reyes, Víctor Manuel Pérez-Sánchez, Héctor Aquiles Maldonado-Martínez, Maybelline Robles-Estrada, Enrique Bargalló-Rocha, Paula Cabrera-Galeana, Maritza Ramos-Ramírez, Yolanda Irasema Chirino, Luis Alonso Herrera, Luis Ignacio Terrazas, Javier Oliver, Cecilia Frecha, Sandra Perdomo, Felipe Vaca-Paniagua. Comprehensive Genomic Profile of Heterogeneous Long Follow-Up Triple-Negative Breast Cancer and Its Clinical Characteristics Shows DNA Repair Deficiency Has Better Prognostic. Genes. 2020; 11 (11):1367.

Chicago/Turabian Style

Ernesto Rojas-Jiménez; Javier César Mejía-Gómez; Clara Díaz-Velásquez; Rosalía Quezada-Urban; Héctor Martínez Gregorio; Fernando Vallejo-Lecuona; Aldo De La Cruz-Montoya; Fany Iris Porras Reyes; Víctor Manuel Pérez-Sánchez; Héctor Aquiles Maldonado-Martínez; Maybelline Robles-Estrada; Enrique Bargalló-Rocha; Paula Cabrera-Galeana; Maritza Ramos-Ramírez; Yolanda Irasema Chirino; Luis Alonso Herrera; Luis Ignacio Terrazas; Javier Oliver; Cecilia Frecha; Sandra Perdomo; Felipe Vaca-Paniagua. 2020. "Comprehensive Genomic Profile of Heterogeneous Long Follow-Up Triple-Negative Breast Cancer and Its Clinical Characteristics Shows DNA Repair Deficiency Has Better Prognostic." Genes 11, no. 11: 1367.

Journal article
Published: 19 November 2020 in Chemosphere
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Air pollution is a worldwide problem that affects human health predominantly in the largest cities. Particulate matter of 10 μm or less in diameter (PM10) is considered a risk factor for multiple diseases, including lung cancer. The long non-coding RNA NORAD and the components of the spindle assembly checkpoint (SAC) ensure proper chromosomal segregation. Alterations in the SAC cause aneuploidy, a feature associated with carcinogenesis. In this study, we demonstrated that PM10 treatment increased the expression levels of NORAD as well as those of SAC components mitotic arrest deficient 1 (MAD1L1), mitotic arrest deficient 2 (MAD2L1), BubR1 (BUB1B), aurora B (AURKB), and survivin (BIRC5) in the lung A549 cell line. We also demonstrated that MAD1L1, MAD2L1, and BUB1B expression levels were reduced when cells were transfected with small interfering RNAs (siRNAs) against NORAD. Interestingly, the expression levels of AURKB and BIRC5 (survivin) were not affected by transfection with NORAD siRNAs. Cells treated with PM10 exhibited a decrease in mitotic arrest and an increase in micronuclei frequency in synchronized A549 cells. PM10 exposure induced aneuploidy events as a result of SAC deregulation. We also observed a reduction in the protein levels of Pumilio 1 after PM10 treatment. Our results provide novel clues regarding the effect of PM10 in the generation of chromosomal instability, a phenotype observed in lung cancer cells.

ACS Style

Miguel Santibáñez-Andrade; Yesennia Sánchez-Pérez; Yolanda I. Chirino; Rocío Morales-Bárcenas; Claudia M. García-Cuellar. Long non-coding RNA NORAD upregulation induced by airborne particulate matter (PM10) exposure leads to aneuploidy in A549 lung cells. Chemosphere 2020, 266, 128994 .

AMA Style

Miguel Santibáñez-Andrade, Yesennia Sánchez-Pérez, Yolanda I. Chirino, Rocío Morales-Bárcenas, Claudia M. García-Cuellar. Long non-coding RNA NORAD upregulation induced by airborne particulate matter (PM10) exposure leads to aneuploidy in A549 lung cells. Chemosphere. 2020; 266 ():128994.

Chicago/Turabian Style

Miguel Santibáñez-Andrade; Yesennia Sánchez-Pérez; Yolanda I. Chirino; Rocío Morales-Bárcenas; Claudia M. García-Cuellar. 2020. "Long non-coding RNA NORAD upregulation induced by airborne particulate matter (PM10) exposure leads to aneuploidy in A549 lung cells." Chemosphere 266, no. : 128994.

Journal article
Published: 02 August 2020 in Toxicology
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The Organisation for Economic Co-operation and Development has listed thirteen engineered nanomaterials (ENM) in order to investigate their toxicity on human health. Silicon dioxide (SiO2) and titanium dioxide (TiO2) are included on that list and we added indium tin oxide (ITO) nanoparticles (NPs) to our study, which is not listed on OECD suggested ENM to be investigated, however ITO NPs has a high potential of industrial production. We evaluate the physicochemical properties of SiO2 NPs (10−20 nm), TiO2 nanofibers (NFs; 3 μm length) and ITO NPs (<50 nm) and the impact of protein-corona formation on cell internalization. Then, we evaluated the toxicity of uncoated ENM on human lung epithelial cells exposed to 10 and 50 μg/cm2 for 24 h. TiO2 NFs showed the highest capability to adsorb proteins onto the particle surface followed by SiO2 NPs and ITO NPs after acellular incubation with fetal bovine serum. The protein adsorption had no impact on Alizarin Red S conjugation, intrinsic properties for reactive oxygen (ROS) formation or cell uptake for all types of ENM. Moreover, TiO2 NFs induced highest cell alterations in human lung epithelial cells exposed to 10 and 50 μg/cm2 while ITO NPs induced moderated cytotoxicity and SiO2 NPs caused even lower cytotoxicity under the same conditions. DNA, proteins and lipids were mainly affected by TiO2 NFs followed by SiO2 NPs with toxic effects in protein and lipids while limited variations were detected after exposure to ITO NPs on spectra analyzed by Fourier Transform Infrared Spectroscopy.

ACS Style

Alejandro Déciga-Alcaraz; Estefany I. Medina-Reyes; Norma L. Delgado-Buenrostro; Carolina Rodriguez Ibarra; Adriana Ganem-Rondero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda; Jorge H. Limón-Pacheco; Claudia M. García-Cuéllar; Yesennia Sánchez-Pérez; Yolanda I. Chirino. Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production. Toxicology 2020, 442, 152545 .

AMA Style

Alejandro Déciga-Alcaraz, Estefany I. Medina-Reyes, Norma L. Delgado-Buenrostro, Carolina Rodriguez Ibarra, Adriana Ganem-Rondero, Gustavo Jesus Vazquez-Zapien, Monica Maribel Mata-Miranda, Jorge H. Limón-Pacheco, Claudia M. García-Cuéllar, Yesennia Sánchez-Pérez, Yolanda I. Chirino. Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production. Toxicology. 2020; 442 ():152545.

Chicago/Turabian Style

Alejandro Déciga-Alcaraz; Estefany I. Medina-Reyes; Norma L. Delgado-Buenrostro; Carolina Rodriguez Ibarra; Adriana Ganem-Rondero; Gustavo Jesus Vazquez-Zapien; Monica Maribel Mata-Miranda; Jorge H. Limón-Pacheco; Claudia M. García-Cuéllar; Yesennia Sánchez-Pérez; Yolanda I. Chirino. 2020. "Toxicity of engineered nanomaterials with different physicochemical properties and the role of protein corona on cellular uptake and intrinsic ROS production." Toxicology 442, no. : 152545.

Journal article
Published: 29 July 2020 in Toxics
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Some studies have shown that silicon dioxide nanoparticles (SiO2-NPs) can reach different regions of the brain and cause toxicity; however, the consequences of SiO2-NPs exposure on the diverse brain cell lineages is limited. We aimed to investigate the neurotoxic effects of SiO2-NP (0–100 µg/mL) on rat astrocyte-rich cultures or neuron-rich cultures using scanning electron microscopy, Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR), FTIR microspectroscopy mapping (IQ mapping), and cell viability tests. SiO2-NPs were amorphous particles and aggregated in saline and culture media. Both astrocytes and neurons treated with SiO2-NPs showed alterations in cell morphology and changes in the IR spectral regions corresponding to nucleic acids, proteins, and lipids. The analysis by the second derivative revealed a significant decrease in the signal of the amide I (α-helix, parallel β-strand, and random coil) at the concentration of 10 µg/mL in astrocytes but not in neurons. IQ mapping confirmed changes in nucleic acids, proteins, and lipids in astrocytes; cell death was higher in astrocytes than in neurons (10–100 µg/mL). We conclude that astrocytes were more vulnerable than neurons to SiO2-NPs toxicity. Therefore, the evaluation of human exposure to SiO2-NPs and possible neurotoxic effects must be followed up.

ACS Style

Jorge Humberto Limón-Pacheco; Natalie Jiménez-Barrios; Alejandro Déciga-Alcaraz; Adriana Martínez-Cuazitl; Mónica Maribel Mata-Miranda; Gustavo Jesús Vázquez-Zapién; Jose Pedraza-Chaverri; Yolanda Irasema Chirino; Marisol Orozco-Ibarra. Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro. Toxics 2020, 8, 51 .

AMA Style

Jorge Humberto Limón-Pacheco, Natalie Jiménez-Barrios, Alejandro Déciga-Alcaraz, Adriana Martínez-Cuazitl, Mónica Maribel Mata-Miranda, Gustavo Jesús Vázquez-Zapién, Jose Pedraza-Chaverri, Yolanda Irasema Chirino, Marisol Orozco-Ibarra. Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro. Toxics. 2020; 8 (3):51.

Chicago/Turabian Style

Jorge Humberto Limón-Pacheco; Natalie Jiménez-Barrios; Alejandro Déciga-Alcaraz; Adriana Martínez-Cuazitl; Mónica Maribel Mata-Miranda; Gustavo Jesús Vázquez-Zapién; Jose Pedraza-Chaverri; Yolanda Irasema Chirino; Marisol Orozco-Ibarra. 2020. "Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro." Toxics 8, no. 3: 51.

Corrigendum
Published: 17 June 2020 in Mediators of Inflammation
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In the article titled “Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer” [1], the dot plot in Figure 6(c) for the healthy MIF-/- mice inadvertently duplicated the dot plot for the MIF-/- CRC mice. This was identified by the authors and is corrected by the revised figure shown in Figure 6. Copyright © 2020 Thalia Pacheco-Fernández et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

ACS Style

Thalia Pacheco-Fernández; Imelda Juárez-Avelar; Oscar Illescas; Luis I. Terrazas; Rogelio Hernández-Pando; Carlos Pérez-Plasencia; Emma B. Gutiérrez-Cirlos; Federico Ávila-Moreno; Yolanda I. Chirino; José Luis Reyes; Vilma Maldonado; Miriam Rodriguez-Sosa. Corrigendum to “Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer”. Mediators of Inflammation 2020, 2020, 1 -2.

AMA Style

Thalia Pacheco-Fernández, Imelda Juárez-Avelar, Oscar Illescas, Luis I. Terrazas, Rogelio Hernández-Pando, Carlos Pérez-Plasencia, Emma B. Gutiérrez-Cirlos, Federico Ávila-Moreno, Yolanda I. Chirino, José Luis Reyes, Vilma Maldonado, Miriam Rodriguez-Sosa. Corrigendum to “Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer”. Mediators of Inflammation. 2020; 2020 ():1-2.

Chicago/Turabian Style

Thalia Pacheco-Fernández; Imelda Juárez-Avelar; Oscar Illescas; Luis I. Terrazas; Rogelio Hernández-Pando; Carlos Pérez-Plasencia; Emma B. Gutiérrez-Cirlos; Federico Ávila-Moreno; Yolanda I. Chirino; José Luis Reyes; Vilma Maldonado; Miriam Rodriguez-Sosa. 2020. "Corrigendum to “Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer”." Mediators of Inflammation 2020, no. : 1-2.

Journal article
Published: 26 March 2020 in Chemico-Biological Interactions
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Exposure to TiO2 NPs induces several cellular alterations after NPs uptake including disruption of cytoskeleton that is crucial for lung physiology but is not considered as a footprint of cell damage. We aimed to investigate cytoskeleton disturbances and the impact on cell migration induced by an acute TiO2 NPs exposure (24 h) and the recovery capability after 6 days of NPs-free treatment, which allowed investigating if cytoskeleton damage was reversible. Exposure to TiO2 NPs (10 μg/cm2) for 24 h induced a decrease 20.2% and 25.1% in tubulin and actin polymerization. Exposure to TiO2 NPs (10 μg/cm2) for 24 h followed by 6 days of NPs-free had a decrease of 26.6% and 21.3% in tubulin and actin polymerization, respectively. The sustained exposure for 7 days to 1 μg/cm2 and 10 μg/cm2 induced a decrease of 22.4% and 30.7% of tubulin polymerization respectively, and 28.7% and 46.2% in actin polymerization. In addition, 24 h followed 6 days of NPs-free exposure of TiO2 NPs (1 μg/cm2 and 10 μg/cm2) decreased cell migration 40.7% and 59.2%, respectively. Cells exposed (10 μg/cm2) for 7 days had a decrease of 65.5% in cell migration. Ki67, protein surfactant B (SFTPB) and matrix metalloprotease 2 (MMP2) were analyzed as genes related to lung epithelial function. The results showed a 20% of Ki67 upregulation in cells exposed for 24 h to 10 μg/cm2 TiO2 NPs while a downregulation of 20% and 25.8% in cells exposed to 1 μg/cm2 and 10 μg/cm2 for 24 h followed by 6 days of NPs-free exposure. Exposure to 1 μg/cm2 and 10 μg/cm2 for 24 h and 7 days upregulates SFTPB expression in 53% and 59% respectively, MMP2 expression remain unchanged. In conclusion, exposure of TiO2 NPs affected cytoskeleton of lung epithelial cells irreversibly but this damage was not cumulative.

ACS Style

Alejandro Déciga Alcaraz; Norma L. Delgado-Buenrostro; Octavio Ispanixtlahuatl-Meráz; Verónica Freyre-Fonseca; José O. Flores-Flores; Adriana Ganem-Rondero; Felipe Vaca-Paniagua; María Del Pilar Ramos-Godinez; Rocío Morales-Barcenas; Yesennia Sánchez-Pérez; Claudia M. García-Cuéllar; Yolanda I. Chirino. Irreversible disruption of the cytoskeleton as induced by non-cytotoxic exposure to titanium dioxide nanoparticles in lung epithelial cells. Chemico-Biological Interactions 2020, 323, 109063 .

AMA Style

Alejandro Déciga Alcaraz, Norma L. Delgado-Buenrostro, Octavio Ispanixtlahuatl-Meráz, Verónica Freyre-Fonseca, José O. Flores-Flores, Adriana Ganem-Rondero, Felipe Vaca-Paniagua, María Del Pilar Ramos-Godinez, Rocío Morales-Barcenas, Yesennia Sánchez-Pérez, Claudia M. García-Cuéllar, Yolanda I. Chirino. Irreversible disruption of the cytoskeleton as induced by non-cytotoxic exposure to titanium dioxide nanoparticles in lung epithelial cells. Chemico-Biological Interactions. 2020; 323 ():109063.

Chicago/Turabian Style

Alejandro Déciga Alcaraz; Norma L. Delgado-Buenrostro; Octavio Ispanixtlahuatl-Meráz; Verónica Freyre-Fonseca; José O. Flores-Flores; Adriana Ganem-Rondero; Felipe Vaca-Paniagua; María Del Pilar Ramos-Godinez; Rocío Morales-Barcenas; Yesennia Sánchez-Pérez; Claudia M. García-Cuéllar; Yolanda I. Chirino. 2020. "Irreversible disruption of the cytoskeleton as induced by non-cytotoxic exposure to titanium dioxide nanoparticles in lung epithelial cells." Chemico-Biological Interactions 323, no. : 109063.

Journal article
Published: 20 March 2020 in International Journal of Molecular Sciences
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Colorectal cancer (CRC) is one of the most widespread and deadly types of neoplasia around the world, where the inflammatory microenvironment has critical importance in the process of tumor growth, metastasis, and drug resistance. Despite its limited effectiveness, 5-fluorouracil (5-FU) is the main drug utilized for CRC treatment. The combination of 5-FU with other agents modestly increases its effectiveness in patients. Here, we evaluated the anti-inflammatory Trimethylglycine and the Signal transducer and activator of transcription (STAT6) inhibitor AS1517499, as possible adjuvants to 5-FU in already established cancers, using a model of colitis-associated colon cancer (CAC). We found that these adjuvant therapies induced a remarkable reduction of tumor growth when administrated together with 5-FU, correlating with a reduction in STAT6-phosphorylation. This reduction upgraded the effect of 5-FU by increasing both levels of apoptosis and markers of cell adhesion such as E-cadherin, whereas decreased epithelial–mesenchymal transition markers were associated with aggressive phenotypes and drug resistance, such as β-catenin nuclear translocation and Zinc finger protein SNAI1 (SNAI1). Additionally, Il-10, Tgf-β, and Il-17a, critical pro-tumorigenic cytokines, were downmodulated in the colon by these adjuvant therapies. In vitro assays on human colon cancer cells showed that Trimethylglycine also reduced STAT6-phosphorylation. Our study is relatively unique in focusing on the effects of the combined administration of AS1517499 and Trimethylglycine together with 5-FU on already established CAC which synergizes to markedly reduce the colon tumor load. Together, these data point to STAT6 as a valuable target for adjuvant therapy in colon cancer.

ACS Style

Monica Mendoza; C. Ángel Sánchez-Barrera; Blanca E. Callejas; Verónica García-Castillo; Diana L. Beristain-Terrazas; Norma L. Delgado-Buenrostro; Yolanda I. Chirino; Sonia A. León-Cabrera; Miriam Rodríguez-Sosa; Emma Bertha Gutierrez-Cirlos; Carlos Pérez-Plasencia; Felipe Vaca-Paniagua; Marco Antonio Meraz-Ríos; Luis I. Terrazas. Use of STAT6 Phosphorylation Inhibitor and Trimethylglycine as New Adjuvant Therapies for 5-Fluorouracil in Colitis-Associated Tumorigenesis. International Journal of Molecular Sciences 2020, 21, 2130 .

AMA Style

Monica Mendoza, C. Ángel Sánchez-Barrera, Blanca E. Callejas, Verónica García-Castillo, Diana L. Beristain-Terrazas, Norma L. Delgado-Buenrostro, Yolanda I. Chirino, Sonia A. León-Cabrera, Miriam Rodríguez-Sosa, Emma Bertha Gutierrez-Cirlos, Carlos Pérez-Plasencia, Felipe Vaca-Paniagua, Marco Antonio Meraz-Ríos, Luis I. Terrazas. Use of STAT6 Phosphorylation Inhibitor and Trimethylglycine as New Adjuvant Therapies for 5-Fluorouracil in Colitis-Associated Tumorigenesis. International Journal of Molecular Sciences. 2020; 21 (6):2130.

Chicago/Turabian Style

Monica Mendoza; C. Ángel Sánchez-Barrera; Blanca E. Callejas; Verónica García-Castillo; Diana L. Beristain-Terrazas; Norma L. Delgado-Buenrostro; Yolanda I. Chirino; Sonia A. León-Cabrera; Miriam Rodríguez-Sosa; Emma Bertha Gutierrez-Cirlos; Carlos Pérez-Plasencia; Felipe Vaca-Paniagua; Marco Antonio Meraz-Ríos; Luis I. Terrazas. 2020. "Use of STAT6 Phosphorylation Inhibitor and Trimethylglycine as New Adjuvant Therapies for 5-Fluorouracil in Colitis-Associated Tumorigenesis." International Journal of Molecular Sciences 21, no. 6: 2130.

Review article
Published: 22 January 2020 in Toxicology Letters
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The increasing concern of possible adverse effects on human health derived from occupational engineered nanomaterials (ENMs) exposure is an issue addressed by entities related to provide guidelines and/or protocols for ENMs regulation. Here we analysed 17 entities from America, Europe and Asia, and some of these entities provide limits of exposure extrapolated from the non-nanosized counterparts of ENMs. The international landscape shows that recommendations are mostly made for metal oxide based ENMs and tonnage is one of the main criteria for ENMs registration, however, sub-nanometric ENMs are emerging and perhaps a novel category of ENMs will appear soon. We identify that besides the lack of epidemiological evidence of ENMs toxicity in humans and difficulties in analysing the toxicological data derived from experimental models, the lack of information on airborne concentrations of ENMs in occupational settings is an important limitation to improve the experimental designs. The development of regulations related to ENMs exposure would lead to provide safer work places for ENMs production without delaying the nanotechnology progress but will also help to protect the environment by taking opportune and correct measures for nanowaste, considering that this could be a great environmental problem in the coming future.

ACS Style

Carolina Rodriguez Ibarra; Alejandro Déciga Alcaraz; Octavio Ispanixtlahuatl-Meráz; Estefany-Ingrid Medina-Reyes; Norma L. Delgado-Buenrostro; Yolanda I. Chirino. International landscape of limits and recommendations for occupational exposure to engineered nanomaterials. Toxicology Letters 2020, 322, 111 -119.

AMA Style

Carolina Rodriguez Ibarra, Alejandro Déciga Alcaraz, Octavio Ispanixtlahuatl-Meráz, Estefany-Ingrid Medina-Reyes, Norma L. Delgado-Buenrostro, Yolanda I. Chirino. International landscape of limits and recommendations for occupational exposure to engineered nanomaterials. Toxicology Letters. 2020; 322 ():111-119.

Chicago/Turabian Style

Carolina Rodriguez Ibarra; Alejandro Déciga Alcaraz; Octavio Ispanixtlahuatl-Meráz; Estefany-Ingrid Medina-Reyes; Norma L. Delgado-Buenrostro; Yolanda I. Chirino. 2020. "International landscape of limits and recommendations for occupational exposure to engineered nanomaterials." Toxicology Letters 322, no. : 111-119.

Journal article
Published: 11 January 2020 in International Journal of Molecular Sciences
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Outdoor particulate matter (PM10) exposure is carcinogenic to humans. The cellular mechanism by which PM10 is associated specifically with lung cancer includes oxidative stress and damage to proteins, lipids, and DNA in the absence of apoptosis, suggesting that PM10 induces cellular survival. We aimed to evaluate the PI3K/AKT/FoxO3a pathway as a mechanism of cell survival in lung epithelial A549 cells exposed to PM10 that were subsequently challenged with hydrogen peroxide (H2O2). Our results showed that pre-exposure to PM10 followed by H2O2, as a second oxidant stimulus increased the phosphorylation rate of pAKTSer473, pAKTThr308, and pFoxO3aSer253 2.5-fold, 1.8-fold, and 1.2-fold, respectively. Levels of catalase and p27kip1, which are targets of the PIK3/AKT/FoxO3a pathway, decreased 38.1% and 62.7%, respectively. None of these changes had an influence on apoptosis; however, the inhibition of PI3K using the LY294002 compound revealed that the PI3K/AKT/FoxO3a pathway was involved in apoptosis evasion. We conclude that nontoxic PM10 exposure predisposes lung epithelial cell cultures to evade apoptosis through the PI3K/AKT/FoxO3a pathway when cells are treated with a second oxidant stimulus.

ACS Style

Claudia M. García-Cuellar; Yolanda I. Chirino; Rocío Morales-Bárcenas; Ernesto Soto-Reyes; Raúl Quintana-Belmares; Miguel Santibáñez-Andrade; Yesennia Sánchez-Pérez. Airborne Particulate Matter (PM10) Inhibits Apoptosis through PI3K/AKT/FoxO3a Pathway in Lung Epithelial Cells: The Role of a Second Oxidant Stimulus. International Journal of Molecular Sciences 2020, 21, 473 .

AMA Style

Claudia M. García-Cuellar, Yolanda I. Chirino, Rocío Morales-Bárcenas, Ernesto Soto-Reyes, Raúl Quintana-Belmares, Miguel Santibáñez-Andrade, Yesennia Sánchez-Pérez. Airborne Particulate Matter (PM10) Inhibits Apoptosis through PI3K/AKT/FoxO3a Pathway in Lung Epithelial Cells: The Role of a Second Oxidant Stimulus. International Journal of Molecular Sciences. 2020; 21 (2):473.

Chicago/Turabian Style

Claudia M. García-Cuellar; Yolanda I. Chirino; Rocío Morales-Bárcenas; Ernesto Soto-Reyes; Raúl Quintana-Belmares; Miguel Santibáñez-Andrade; Yesennia Sánchez-Pérez. 2020. "Airborne Particulate Matter (PM10) Inhibits Apoptosis through PI3K/AKT/FoxO3a Pathway in Lung Epithelial Cells: The Role of a Second Oxidant Stimulus." International Journal of Molecular Sciences 21, no. 2: 473.

Review
Published: 24 December 2019 in International Journal of Molecular Sciences
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Air pollution has been recognized as a global health problem, causing around 7 million deaths worldwide and representing one of the highest environmental crises that we are now facing. Close to 30% of new lung cancer cases are associated with air pollution, and the impact is more evident in major cities. In this review, we summarize and discuss the evidence regarding the effect of particulate matter (PM) and its impact in carcinogenesis, considering the “hallmarks of cancer” described by Hanahan and Weinberg in 2000 and 2011 as a guide to describing the findings that support the impact of particulate matter during the cancer continuum.

ACS Style

Miguel Santibáñez-Andrade; Yolanda I. Chirino; Imelda González-Ramírez; Yesennia Sánchez-Pérez; Claudia M. García-Cuellar. Deciphering the Code between Air Pollution and Disease: The Effect of Particulate Matter on Cancer Hallmarks. International Journal of Molecular Sciences 2019, 21, 136 .

AMA Style

Miguel Santibáñez-Andrade, Yolanda I. Chirino, Imelda González-Ramírez, Yesennia Sánchez-Pérez, Claudia M. García-Cuellar. Deciphering the Code between Air Pollution and Disease: The Effect of Particulate Matter on Cancer Hallmarks. International Journal of Molecular Sciences. 2019; 21 (1):136.

Chicago/Turabian Style

Miguel Santibáñez-Andrade; Yolanda I. Chirino; Imelda González-Ramírez; Yesennia Sánchez-Pérez; Claudia M. García-Cuellar. 2019. "Deciphering the Code between Air Pollution and Disease: The Effect of Particulate Matter on Cancer Hallmarks." International Journal of Molecular Sciences 21, no. 1: 136.

Journal article
Published: 15 August 2019 in Journal of Applied Toxicology
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Food-grade titanium dioxide labeled as E171 has been approved for human consumption by the Food and Drug Administration (USA) and by the European Union for five decades. However, titanium dioxide has been classified as a possible carcinogen for humans by the International Agency of Research in Cancer raising concerns of its oral intake and the translocation to bloodstream, which could disturb barriers such as the blood-testis barrier. There is evidence that titanium dioxide by intragastric/intraperitoneal/intravenous administration induced alterations on testosterone levels, testicular function and architecture, but studies of the E171 effects on the testicle structure and blood-testis barrier are limited. E171 is contained not only in foods in liquid matrix but also in solid ones, which can exert different biological effects. We aimed to compare the effects of E171 consumption in a solid matrix (0.1%, 0.5% and 1% in pellets) and liquid suspension (5 mg/kg body weight) on testis structure, inflammation infiltrate and blood-testis barrier disruption of male BALB/c mice. Results showed that none of the administration routes had influence on body weight but an increase in germ cell sloughing and the infiltrate of inflammatory cells in seminiferous tubules, together with disruption of the blood-testis barrier were similar in testis of both groups even if the dose received in mice in liquid matrix was 136 or 260 times lower than the dose reached by oral intake in solid E171 pellets in 0.5% E171 and 1% E171, respectively. This study highlights the attention on matrix food containing E171 and possible adverse effects on testis when E171 is consumed in a liquid matrix.

ACS Style

Juan Carlos Rodríguez-Escamilla; Estefany I. Medina-Reyes; Carolina Rodríguez-Ibarra; Alejandro Déciga-Alcaraz; José O. Flores-Flores; Adriana Ganem-Rondero; Miriam Rodríguez-Sosa; Luis I. Terrazas; Norma L. Delgado-Buenrostro; Yolanda I. Chirino. Food-grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood-testis barrier disruption in mice. Journal of Applied Toxicology 2019, 39, 1586 -1605.

AMA Style

Juan Carlos Rodríguez-Escamilla, Estefany I. Medina-Reyes, Carolina Rodríguez-Ibarra, Alejandro Déciga-Alcaraz, José O. Flores-Flores, Adriana Ganem-Rondero, Miriam Rodríguez-Sosa, Luis I. Terrazas, Norma L. Delgado-Buenrostro, Yolanda I. Chirino. Food-grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood-testis barrier disruption in mice. Journal of Applied Toxicology. 2019; 39 (11):1586-1605.

Chicago/Turabian Style

Juan Carlos Rodríguez-Escamilla; Estefany I. Medina-Reyes; Carolina Rodríguez-Ibarra; Alejandro Déciga-Alcaraz; José O. Flores-Flores; Adriana Ganem-Rondero; Miriam Rodríguez-Sosa; Luis I. Terrazas; Norma L. Delgado-Buenrostro; Yolanda I. Chirino. 2019. "Food-grade titanium dioxide (E171) by solid or liquid matrix administration induces inflammation, germ cells sloughing in seminiferous tubules and blood-testis barrier disruption in mice." Journal of Applied Toxicology 39, no. 11: 1586-1605.

Research article
Published: 10 July 2019 in Mediators of Inflammation
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Colitis-associated colorectal cancer (CRC) development has been shown to be related to chronically enhanced inflammation. Macrophage migration inhibitory factor (MIF) is an inflammatory mediator that favors inflammatory cytokine production and has chemotactic properties for the recruitment of macrophages (Møs) and T cells. Here, we investigated the role of MIF in the inflammatory response and recruitment of immune cells in a murine model of chemical carcinogenesis to establish the impact of MIF on CRC genesis and malignancy. We used BALB/c MIF-knockout (MIF-/-) and wild-type (WT) mice to develop CRC by administering intraperitoneal (i.p.) azoxymethane and dextran sodium sulfate in drinking water. Greater tumor burdens were observed in MIF-/- mice than in WT mice. Tumors from MIF-/- mice were histologically identified to be more aggressive than tumors from WT mice. The localization of MIF suggests that it is also involved in cell differentiation. The relative gene expression of il-17, measured by real-time PCR, was higher in MIF-/- CRC mice, compared to the WT CRC and healthy MIF-/- mice. Importantly, compared to the WT intestinal epithelium, lower percentages of tumor-associated Møs were found in the MIF-/- intestinal epithelium. These results suggest that MIF plays a role in controlling the initial development of CRC by attracting Møs to the tumor, which is a condition that favors the initial antitumor responses.

ACS Style

Thalia Pacheco-Fernández; Imelda Juárez-Avelar; Oscar Illescas; Luis I. Terrazas; Rogelio Hernández-Pando; Carlos Pérez-Plasencia; Emma B. Gutiérrez-Cirlos; Federico Ávila-Moreno; Yolanda I. Chirino; José Luis Reyes; Vilma Maldonado; Miriam Rodriguez-Sosa. Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer. Mediators of Inflammation 2019, 2019, 1 -16.

AMA Style

Thalia Pacheco-Fernández, Imelda Juárez-Avelar, Oscar Illescas, Luis I. Terrazas, Rogelio Hernández-Pando, Carlos Pérez-Plasencia, Emma B. Gutiérrez-Cirlos, Federico Ávila-Moreno, Yolanda I. Chirino, José Luis Reyes, Vilma Maldonado, Miriam Rodriguez-Sosa. Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer. Mediators of Inflammation. 2019; 2019 ():1-16.

Chicago/Turabian Style

Thalia Pacheco-Fernández; Imelda Juárez-Avelar; Oscar Illescas; Luis I. Terrazas; Rogelio Hernández-Pando; Carlos Pérez-Plasencia; Emma B. Gutiérrez-Cirlos; Federico Ávila-Moreno; Yolanda I. Chirino; José Luis Reyes; Vilma Maldonado; Miriam Rodriguez-Sosa. 2019. "Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer." Mediators of Inflammation 2019, no. : 1-16.

Journal article
Published: 06 July 2019 in Journal of Drug Delivery Science and Technology
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Tetraphenylporphyrin (H2TPP), was synthesized and characterized by UV spectroscopy. H2TPP was included in a solution (Labrasol™:Transcutol™ 1:2 v/v) and in a microemulsion (whose composition was chosen from the construction of pseudo-ternary diagrams). Permeation experiments were performed with these two systems through intact skin and skin pretreated with sonophoresis. Confocal laser scanning microscopy was useful to evidence that in all cases, H2TPP penetrated the interior of the skin, increasing the amount that penetrates when the skin was treated with sonophoresis, especially for the combination sonophoresis/microemulsion, with which the highest permeated amount was achieved. In no case, H2TPP was found in the receptor medium, which may be desirable if the aim is to reduce a systemic effect. The results obtained suggest that the proposed systems have potential application in photodynamic therapy, whose effectiveness depends on the penetration and accumulation of photosensitizer in the affected tissue.

ACS Style

R.I.Y. Quiroz-Segoviano; M.A. García-Sánchez; N.L. Delgado-Buenrostro; Y.I. Chirino; S. Bernal-Chávez; M.G. Nava-Arzaluz; A. Ganem-Rondero. Tetraphenylporphyrin intended for use in photodynamic therapy: Influence of sonophoresis and the formulation (solution or microemulsion) on percutaneous penetration. Journal of Drug Delivery Science and Technology 2019, 53, 101145 .

AMA Style

R.I.Y. Quiroz-Segoviano, M.A. García-Sánchez, N.L. Delgado-Buenrostro, Y.I. Chirino, S. Bernal-Chávez, M.G. Nava-Arzaluz, A. Ganem-Rondero. Tetraphenylporphyrin intended for use in photodynamic therapy: Influence of sonophoresis and the formulation (solution or microemulsion) on percutaneous penetration. Journal of Drug Delivery Science and Technology. 2019; 53 ():101145.

Chicago/Turabian Style

R.I.Y. Quiroz-Segoviano; M.A. García-Sánchez; N.L. Delgado-Buenrostro; Y.I. Chirino; S. Bernal-Chávez; M.G. Nava-Arzaluz; A. Ganem-Rondero. 2019. "Tetraphenylporphyrin intended for use in photodynamic therapy: Influence of sonophoresis and the formulation (solution or microemulsion) on percutaneous penetration." Journal of Drug Delivery Science and Technology 53, no. : 101145.

Journal article
Published: 26 November 2018 in Environmental Science: Nano
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Lung adenocarcinoma cells exposed to TiO2nanofibers enhanced tumor characteristics including angiogenic markers and genomic instability, and these cells can even acquire a more aggressive phenotype when grown in a xenograft nude mouse model.

ACS Style

Estefany Ingrid Medina-Reyes; Norma Laura Delgado-Buenrostro; Alejandro Déciga-Alcaraz; Verónica Freyre-Fonseca; José Ocotlán Flores-Flores; Rogelio Hernández-Pando; Jorge Barrios-Payán; Julio Cesar Carrero; Yesennia Sánchez-Pérez; Claudia María García-Cuéllar; Felipe Vaca-Paniagua; Yolanda Irasema Chirino. Titanium dioxide nanofibers induce angiogenic markers and genomic instability in lung cells leading to a highly dedifferentiated and fibrotic tumor formation in a xenograft model. Environmental Science: Nano 2018, 6, 286 -304.

AMA Style

Estefany Ingrid Medina-Reyes, Norma Laura Delgado-Buenrostro, Alejandro Déciga-Alcaraz, Verónica Freyre-Fonseca, José Ocotlán Flores-Flores, Rogelio Hernández-Pando, Jorge Barrios-Payán, Julio Cesar Carrero, Yesennia Sánchez-Pérez, Claudia María García-Cuéllar, Felipe Vaca-Paniagua, Yolanda Irasema Chirino. Titanium dioxide nanofibers induce angiogenic markers and genomic instability in lung cells leading to a highly dedifferentiated and fibrotic tumor formation in a xenograft model. Environmental Science: Nano. 2018; 6 (1):286-304.

Chicago/Turabian Style

Estefany Ingrid Medina-Reyes; Norma Laura Delgado-Buenrostro; Alejandro Déciga-Alcaraz; Verónica Freyre-Fonseca; José Ocotlán Flores-Flores; Rogelio Hernández-Pando; Jorge Barrios-Payán; Julio Cesar Carrero; Yesennia Sánchez-Pérez; Claudia María García-Cuéllar; Felipe Vaca-Paniagua; Yolanda Irasema Chirino. 2018. "Titanium dioxide nanofibers induce angiogenic markers and genomic instability in lung cells leading to a highly dedifferentiated and fibrotic tumor formation in a xenograft model." Environmental Science: Nano 6, no. 1: 286-304.

Journal article
Published: 27 September 2018 in Cancers
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Hereditary breast and ovarian cancer syndrome (HBOC) represents 5–10% of all patients with breast cancer and is associated with high-risk pathogenic alleles in BRCA1/2 genes, but only for 25% of cases. We aimed to find new pathogenic alleles in a panel of 143 cancer-predisposing genes in 300 Mexican cancer patients with suspicion of HBOC and 27 high-risk patients with a severe family history of cancer, using massive parallel sequencing. We found pathogenic variants in 23 genes, including BRCA1/2. In the group of cancer patients 15% (46/300) had a pathogenic variant; 11% (33/300) harbored variants with unknown clinical significance (VUS) and 74% (221/300) were negative. The high-risk group had 22% (6/27) of patients with pathogenic variants, 4% (1/27) had VUS and 74% (20/27) were negative. The most recurrent mutations were the Mexican founder deletion of exons 9-12 and the variant p.G228fs in BRCA1, each found in 5 of 17 patients with alterations in this gene. Rare VUS with potential impact at the protein level were found in 21 genes. Our results show for the first time in the Mexican population a higher contribution of pathogenic alleles in other susceptibility cancer genes (54%) than in BRCA1/2 (46%), highlighting the high locus heterogeneity of HBOC and the necessity of expanding genetic tests for this disease to include broader gene panels.

ACS Style

Rosalía Quezada Urban; Clara Estela Díaz Velásquez; Rina Gitler; María Patricia Rojo Castillo; Max Sirota Toporek; Andrea Figueroa Morales; Oscar Moreno García; Lizbeth García Esquivel; Gabriela Torres Mejía; Michael Dean; Iván Delgado Enciso; Héctor Ochoa Díaz López; Fernando Rodríguez León; Virginia Jan; Víctor Hugo Garzón Barrientos; Pablo Ruiz Flores; Perla Karina Espino Silva; Jorge Haro Santa Cruz; Héctor Martínez Gregorio; Ernesto Arturo Rojas Jiménez; Luis Enrique Romero Cruz; Claudia Fabiola Méndez-Catalá; Rosa María Álvarez Gómez; Verónica Fragoso Ontiveros; Luis Alonso Herrera; Isabelle Romieu; Luis Ignacio Terrazas; Yolanda Irasema Chirino; Cecilia Frecha; Javier Oliver; Sandra Perdomo; Felipe Vaca Paniagua. Comprehensive Analysis of Germline Variants in Mexican Patients with Hereditary Breast and Ovarian Cancer Susceptibility. Cancers 2018, 10, 361 .

AMA Style

Rosalía Quezada Urban, Clara Estela Díaz Velásquez, Rina Gitler, María Patricia Rojo Castillo, Max Sirota Toporek, Andrea Figueroa Morales, Oscar Moreno García, Lizbeth García Esquivel, Gabriela Torres Mejía, Michael Dean, Iván Delgado Enciso, Héctor Ochoa Díaz López, Fernando Rodríguez León, Virginia Jan, Víctor Hugo Garzón Barrientos, Pablo Ruiz Flores, Perla Karina Espino Silva, Jorge Haro Santa Cruz, Héctor Martínez Gregorio, Ernesto Arturo Rojas Jiménez, Luis Enrique Romero Cruz, Claudia Fabiola Méndez-Catalá, Rosa María Álvarez Gómez, Verónica Fragoso Ontiveros, Luis Alonso Herrera, Isabelle Romieu, Luis Ignacio Terrazas, Yolanda Irasema Chirino, Cecilia Frecha, Javier Oliver, Sandra Perdomo, Felipe Vaca Paniagua. Comprehensive Analysis of Germline Variants in Mexican Patients with Hereditary Breast and Ovarian Cancer Susceptibility. Cancers. 2018; 10 (10):361.

Chicago/Turabian Style

Rosalía Quezada Urban; Clara Estela Díaz Velásquez; Rina Gitler; María Patricia Rojo Castillo; Max Sirota Toporek; Andrea Figueroa Morales; Oscar Moreno García; Lizbeth García Esquivel; Gabriela Torres Mejía; Michael Dean; Iván Delgado Enciso; Héctor Ochoa Díaz López; Fernando Rodríguez León; Virginia Jan; Víctor Hugo Garzón Barrientos; Pablo Ruiz Flores; Perla Karina Espino Silva; Jorge Haro Santa Cruz; Héctor Martínez Gregorio; Ernesto Arturo Rojas Jiménez; Luis Enrique Romero Cruz; Claudia Fabiola Méndez-Catalá; Rosa María Álvarez Gómez; Verónica Fragoso Ontiveros; Luis Alonso Herrera; Isabelle Romieu; Luis Ignacio Terrazas; Yolanda Irasema Chirino; Cecilia Frecha; Javier Oliver; Sandra Perdomo; Felipe Vaca Paniagua. 2018. "Comprehensive Analysis of Germline Variants in Mexican Patients with Hereditary Breast and Ovarian Cancer Susceptibility." Cancers 10, no. 10: 361.

Journal article
Published: 19 September 2018 in Cancers
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Signal transducer and activator of transcription 1 (STAT1) is part of the Janus kinase (JAK/STAT) signaling pathway that controls critical events in intestinal immune function related to innate and adaptive immunity. Recent studies have implicated STAT1 in tumor–stroma interactions, and its expression and activity are perturbed during colon cancer. However, the role of STAT1 during the initiation of inflammation-associated cancer is not clearly understood. To determine the role of STAT1 in colitis-associated colorectal cancer (CAC), we analyzed the tumor development and kinetics of cell recruitment in wild-type WT or STAT1−/− mice treated with azoxymethane (AOM) and dextran sodium sulfate (DSS). Following CAC induction, STAT1−/− mice displayed an accelerated appearance of inflammation and tumor formation, and increased damage and scores on the disease activity index (DAI) as early as 20 days after AOM-DSS exposure compared to their WT counterparts. STAT1−/− mice showed elevated colonic epithelial cell proliferation in early stages of injury-induced tumor formation and decreased apoptosis in advanced tumors with over-expression of the anti-apoptotic protein Bcl2 at the colon. STAT1−/− mice showed increased accumulation of Ly6G+Ly6C−CD11b+ cells in the spleen at 20 days of CAC development with concomitant increases in the production of IL-17A, IL-17F, and IL-22 cytokines compared to WT mice. Our findings suggest that STAT1 plays a role as a tumor suppressor molecule in inflammation-associated carcinogenesis, particularly during the very early stages of CAC initiation, modulating immune responses as well as controlling mechanisms such as apoptosis and cell proliferation.

ACS Style

Sonia Leon-Cabrera; Armando Vázquez-Sandoval; Emmanuel Molina-Guzman; Yael Delgado-Ramirez; Norma L. Delgado-Buenrostro; Blanca E. Callejas; Yolanda I. Chirino; Carlos Pérez-Plasencia; Miriam Rodríguez-Sosa; Jonadab E. Olguín; Citlaltepetl Salinas; Abhay R. Satoskar; Luis I. Terrazas. Deficiency in STAT1 Signaling Predisposes Gut Inflammation and Prompts Colorectal Cancer Development. Cancers 2018, 10, 341 .

AMA Style

Sonia Leon-Cabrera, Armando Vázquez-Sandoval, Emmanuel Molina-Guzman, Yael Delgado-Ramirez, Norma L. Delgado-Buenrostro, Blanca E. Callejas, Yolanda I. Chirino, Carlos Pérez-Plasencia, Miriam Rodríguez-Sosa, Jonadab E. Olguín, Citlaltepetl Salinas, Abhay R. Satoskar, Luis I. Terrazas. Deficiency in STAT1 Signaling Predisposes Gut Inflammation and Prompts Colorectal Cancer Development. Cancers. 2018; 10 (9):341.

Chicago/Turabian Style

Sonia Leon-Cabrera; Armando Vázquez-Sandoval; Emmanuel Molina-Guzman; Yael Delgado-Ramirez; Norma L. Delgado-Buenrostro; Blanca E. Callejas; Yolanda I. Chirino; Carlos Pérez-Plasencia; Miriam Rodríguez-Sosa; Jonadab E. Olguín; Citlaltepetl Salinas; Abhay R. Satoskar; Luis I. Terrazas. 2018. "Deficiency in STAT1 Signaling Predisposes Gut Inflammation and Prompts Colorectal Cancer Development." Cancers 10, no. 9: 341.

Journal article
Published: 01 January 2018 in Food and Chemical Toxicology
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Dietary factors that may influence the risks of colorectal cancer, including specific supplements, are under investigation. Previous studies showed the capacity of food additive titanium dioxide (E171) to induce DNA damage in vitro and facilitate growth of colorectal tumours in vivo. This study aimed to investigate the molecular mechanisms behind these effects after E171 exposure. BALB/c mice were exposed by gavage to 5 mg/kg/day of E171 for 2, 7, 14, and 21 days. Transcriptome changes were studied by whole genome mRNA microarray analysis on the mice's distal colons. In addition, histopathological changes as well as a proliferation marker were analysed. The results showed significant gene expression changes in the olfactory/GPCR receptor family, oxidative stress, the immune system and of cancer related genes. Transcriptome analysis also identified genes that thus far have not been included in known biological pathways and can induce functional changes by interacting with other genes involved in different biological pathways. Histopathological analysis showed alteration and disruption in the normal structure of crypts inducing a hyperplastic epithelium. At cell proliferation level, no consistent increase over time was observed. These results may offer a mechanistic framework for the enhanced tumour growth after ingestion of E171 in BALB/c mice.

ACS Style

Héloïse Proquin; Marlon J. Jetten; Marloes Jonkhout; Luis G. Garduño-Balderas; Jacco Briedé; Theo M. de Kok; Yolanda I. Chirino; Henk van Loveren. Gene expression profiling in colon of mice exposed to food additive titanium dioxide (E171). Food and Chemical Toxicology 2018, 111, 153 -165.

AMA Style

Héloïse Proquin, Marlon J. Jetten, Marloes Jonkhout, Luis G. Garduño-Balderas, Jacco Briedé, Theo M. de Kok, Yolanda I. Chirino, Henk van Loveren. Gene expression profiling in colon of mice exposed to food additive titanium dioxide (E171). Food and Chemical Toxicology. 2018; 111 ():153-165.

Chicago/Turabian Style

Héloïse Proquin; Marlon J. Jetten; Marloes Jonkhout; Luis G. Garduño-Balderas; Jacco Briedé; Theo M. de Kok; Yolanda I. Chirino; Henk van Loveren. 2018. "Gene expression profiling in colon of mice exposed to food additive titanium dioxide (E171)." Food and Chemical Toxicology 111, no. : 153-165.